Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,274,740 G→A 5.1% intergenic (+79/+20) ssb → / ← yjcB single‑stranded DNA‑binding protein/putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,274,7400GA 5.1% 541.3 / 10.6 176intergenic (+79/+20)ssb/yjcBsingle‑stranded DNA‑binding protein/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (69/94);  new base A (6/3);  total (76/100)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

Only 100 of 176 total aligned reads displayed.

GCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACA  >  NC_000913/4274592‑4274888
                                                                                                                                                    |                                                                                                                                                    
gcagcaGTCCGCTCCGGCAGCGCCGTGAAACGAGCCGCCGATGGAATTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGt                                                                                                                                                     <  1:793178/150‑1 (MQ=255)
       tCCTCTCCTTCATCGCCGTCTAACGAGACGCAGATGGAATTTGATTATGACATTCAGTTATGATTTGTAATTAAAACTATAGTTTATATTGTTTTAAGGTGGATGATTAAAGAATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAg                                                                                                                                              <  2:1731831/150‑1 (MQ=255)
       tCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAg                                                                                                                                              >  2:1312613/1‑150 (MQ=255)
         cGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGc                                                                                                                                            <  1:544439/150‑1 (MQ=255)
             ccGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCATAACGGAGGCTTCt                                                                                                                                        <  2:186812/150‑1 (MQ=255)
             ccGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGACTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCt                                                                                                                                        <  1:1326312/150‑1 (MQ=255)
              cGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATTATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTa                                                                                                                                       <  1:148208/150‑1 (MQ=255)
              cGGCAGCGCCGTCTAACGAGCCGCCGAAGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTATTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTa                                                                                                                                       <  1:158378/150‑1 (MQ=255)
                 cAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCATAACGGAGGCTTCttttt                                                                                                                                    <  1:1621331/150‑5 (MQ=255)
                    cgcCGTCTAACGACCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATc                                                                                                                                 <  1:1679191/150‑1 (MQ=255)
                     gcCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCa                                                                                                                                >  1:653264/1‑150 (MQ=255)
                      ccGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAg                                                                                                                               <  2:622564/150‑1 (MQ=255)
                       cGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAgg                                                                                                                              <  1:728715/150‑1 (MQ=255)
                       cGTCTAACGAGCCGCCGATGGACTCTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAgg                                                                                                                              <  2:691280/150‑1 (MQ=255)
                       cGTCAAACGCGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAgg                                                                                                                              <  2:462624/150‑1 (MQ=255)
                           tAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCaa                                                                                                                          <  2:1586858/150‑1 (MQ=255)
                               gAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATc                                                                                                                      <  1:1405674/150‑1 (MQ=255)
                                   cgccgATGGACTTTGATGATGACATTCCGTTCTGATTTGTGATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTg                                                                                                                  <  1:281675/150‑1 (MQ=255)
                                   cgccgATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTg                                                                                                                  <  2:1668165/150‑1 (MQ=255)
                                   cgccgATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTg                                                                                                                  >  1:1783399/1‑150 (MQ=255)
                                    gccgATGGTCTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATACGTTATATTGTTTTAATGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGTGGCTTCTACGTATCATGTCAAAATCATTgg                                                                                                                 <  2:213037/150‑1 (MQ=255)
                                     ccgATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGc                                                                                                                <  1:564881/150‑1 (MQ=255)
                                      cgATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGcc                                                                                                               <  2:605597/150‑1 (MQ=255)
                                          ggACTTTGATGATGACATTCCTTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATtg                                                                                                           <  2:1688467/150‑1 (MQ=255)
                                          ggAATTTGATGATGACATTCCGTTCTGATTTTTCATTTAAACAATAGGTTATATTGTTTTAAGGGGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATtg                                                                                                           <  1:91175/150‑1 (MQ=255)
                                           gACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATtgt                                                                                                          >  2:908370/1‑150 (MQ=255)
                                             cTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATtgtag                                                                                                        >  1:421979/1‑148 (MQ=255)
                                             cTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTgg                                                                                                        >  2:1346093/1‑150 (MQ=255)
                                             cTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTgg                                                                                                        >  2:689981/1‑150 (MQ=255)
                                              tttGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGTATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGc                                                                                                       <  2:1549618/150‑1 (MQ=255)
                                              tttGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGc                                                                                                       <  2:1804556/150‑1 (MQ=255)
                                              tttGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGc                                                                                                       <  2:862493/150‑1 (MQ=255)
                                              tttGACGATGACAATCCGTTCTGATTTGTCATTAAATCAATAGGGTATATTGTTTTAAGGTGGATTATTAAAGCAGCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAACTCATTGGCCATTGTGGc                                                                                                       <  1:978169/150‑1 (MQ=255)
                                                tgatgatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGGCAAAATCATTGGCCATTGTGGCgg                                                                                                     <  1:369020/150‑1 (MQ=255)
                                                  atgatgaCATTCCGTTCTGATTTGTCATTAAAACAATGGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGgtg                                                                                                   >  1:1655455/1‑150 (MQ=255)
                                                  atgatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGgtg                                                                                                   <  2:1770066/150‑1 (MQ=255)
                                                   tgatgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGgtgt                                                                                                  >  1:1490737/1‑150 (MQ=255)
                                                    tatgaCATTGCGTTCTGATTTGTCATTAATACATTAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGACAGCCATAAAAAAGAAGCCTCCTTTATGGAGGCTTCTACGTTTCAGGTCAAAATCATTGTCCATTGTGGCGGTGTc                                                                                                 <  1:891420/149‑1 (MQ=255)
                                                     atgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCt                                                                                                <  2:783186/150‑1 (MQ=255)
                                                      tgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTg                                                                                               <  1:623061/150‑1 (MQ=255)
                                                      tgaCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTg                                                                                               >  2:459786/1‑150 (MQ=255)
                                                        aCATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCATCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGTCCATTGTGGCGGTGTCTGAt                                                                                             >  2:1562860/1‑150 (MQ=255)
                                                           ttCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCa                                                                                          <  1:612177/150‑1 (MQ=255)
                                                              cGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCAtct                                                                                       >  2:1254878/1‑150 (MQ=255)
                                                              cGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCAtct                                                                                       >  1:233914/1‑150 (MQ=255)
                                                                  cTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCtcttc                                                                                   >  2:412877/1‑150 (MQ=255)
                                                                    gATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTa                                                                                 >  1:1997582/1‑150 (MQ=255)
                                                                       ttGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTatca                                                                              <  2:82582/150‑1 (MQ=255)
                                                                       ttGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTatca                                                                              >  1:263005/1‑150 (MQ=255)
                                                                         gTCATTAAAACTATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGGCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTatcatc                                                                            <  1:1473244/150‑1 (MQ=255)
                                                                          tCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTAtcatca                                                                           <  1:31135/150‑1 (MQ=255)
                                                                          accttAAAACAATATAACCTATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTGCGTTATGGAGGCTTCGACGTTTCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTAtcatca                                                                           >  2:1040462/4‑150 (MQ=255)
                                                                           cATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCAc                                                                          >  2:722322/1‑150 (MQ=255)
                                                                           cATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCAc                                                                          >  2:1138467/1‑150 (MQ=255)
                                                                            aTTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCAcc                                                                         <  2:1226645/150‑1 (MQ=255)
                                                                             ttAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCg                                                                        >  1:1609990/1‑150 (MQ=255)
                                                                             ttAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGACATTGTGGCGGTGTCTGATTCATATCTTCTATCATCAcag                                                                        >  1:100908/1‑148 (MQ=255)
                                                                              tAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGa                                                                       >  2:231544/1‑150 (MQ=255)
                                                                              tAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTAAGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGa                                                                       >  1:269364/1‑150 (MQ=255)
                                                                               aaaaCAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGAc                                                                      <  2:433172/150‑1 (MQ=255)
                                                                                    aaTAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGAt                                                                 >  1:126399/1‑150 (MQ=255)
                                                                                      tAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGAtgt                                                               <  1:1085162/150‑1 (MQ=255)
                                                                                      tAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGAtgt                                                               >  1:176534/1‑150 (MQ=255)
                                                                                      aaGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGAtgt                                                               <  1:1210400/149‑1 (MQ=255)
                                                                                              atTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACAGACTCGATGTTGTGCCaa                                                       >  2:833221/1‑149 (MQ=255)
                                                                                                   tttAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGc                                                  >  2:378510/1‑150 (MQ=255)
                                                                                                     tAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCgg                                                <  2:502182/150‑1 (MQ=255)
                                                                                                     tAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCgg                                                >  2:649043/1‑150 (MQ=255)
                                                                                                      aaGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGt                                               <  2:1609217/150‑1 (MQ=255)
                                                                                                       aGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTg                                              <  2:263005/150‑1 (MQ=255)
                                                                                                          tGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATg                                           <  1:412152/150‑1 (MQ=255)
                                                                                                           ggATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGt                                          >  2:571968/1‑150 (MQ=255)
                                                                                                                 ttAATGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATAATTGGCCATTGTGGCTGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCa                                    <  1:738067/150‑1 (MQ=255)
                                                                                                                   aaaGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCCCCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCAtt                                  >  1:1033508/1‑150 (MQ=255)
                                                                                                                   aaaGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCAtt                                  >  1:1914306/1‑150 (MQ=255)
                                                                                                                   aaaGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACAGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCAtt                                  >  2:1074481/1‑150 (MQ=255)
                                                                                                                    aaGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATtg                                 <  2:1655455/150‑1 (MQ=255)
                                                                                                                     aGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATtgt                                <  1:606369/150‑1 (MQ=255)
                                                                                                                          cTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGc                           <  2:1490737/150‑1 (MQ=255)
                                                                                                                           tGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTGGATGTTGTGCCAGTTAGCGGTGATGTCCGCCATTGTGATGcc                          >  2:347768/1‑150 (MQ=255)
                                                                                                                            gccagccaTAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCa                         <  1:459786/150‑1 (MQ=255)
                                                                                                                             ccagccaTAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCAGCCATTGTGATGCCaa                        >  2:914544/1‑150 (MQ=255)
                                                                                                                              cagccaTAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAg                       <  1:583327/150‑1 (MQ=255)
                                                                                                                               agccaTAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGc                      <  1:972657/150‑1 (MQ=255)
                                                                                                                                   aTAAAAAAGAAGCCTCCATTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAAcc                  >  2:1097470/1‑150 (MQ=255)
                                                                                                                                     aaaaaaGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAAcccc                >  1:1964225/1‑150 (MQ=255)
                                                                                                                                       cgtagaagCCTCCATAACGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATATCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATGGTGATGACAAGCAACCCCAt              >  1:1996862/4‑150 (MQ=255)
                                                                                                                                        aaaGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCAtt             >  2:1314952/1‑150 (MQ=255)
                                                                                                                                         aagaagCCTCCGTTATGGATGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTg            <  1:1346093/150‑1 (MQ=255)
                                                                                                                                         aagaagCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTg            <  1:1108404/150‑1 (MQ=255)
                                                                                                                                         aagaagCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTg            <  1:689981/150‑1 (MQ=255)
                                                                                                                                         aagaagCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTg            <  1:1312613/150‑1 (MQ=255)
                                                                                                                                         aagaagCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGAAGTCCGCCATTGTGATGCCAAGCAACCCCATTg            >  1:1835612/1‑150 (MQ=255)
                                                                                                                                          agtagCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGc           <  1:1254878/147‑1 (MQ=255)
                                                                                                                                           gaagCCTCCATAACGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCACATTGCg          >  2:1534922/1‑150 (MQ=255)
                                                                                                                                              gCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCTTTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAAc       <  2:194972/150‑1 (MQ=255)
                                                                                                                                              gCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAAc       >  2:603879/1‑150 (MQ=255)
                                                                                                                                               ccTCCGTGATGGAGGCTTCTACGTATCCGGTCAAACTCATTGGCCATTGTGTCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAAcc      <  1:124514/150‑1 (MQ=255)
                                                                                                                                                cTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCa     >  2:2003787/1‑150 (MQ=255)
                                                                                                                                                   cGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACa  <  1:412877/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAGCAGTCCGCTCCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTGATTTGTCATTAAAACAATAGGTTATATTGTTTTAAGGTGGATGATTAAAGCATCTGCCAGCCATAAAAAAGAAGCCTCCGTTATGGAGGCTTCTACGTATCAGGTCAAAATCATTGGCCATTGTGGCGGTGTCTGATTCATCTCTTCTATCATCACCGACTCGATGTTGTGCCAGATAGCGGTGATGTCCGCCATTGTGATGCCAAGCAACCCCATTGCGAACCAACA  >  NC_000913/4274592‑4274888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: