Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 429,554 A→T 5.4% Y17F (TAT→TTT)  secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,5540AT 5.4% 404.8 / 9.1 140Y17F (TAT→TTT) secFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base A (78/52);  new base T (3/5);  total (81/59)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.54e-01

Only 100 of 141 total aligned reads displayed.

GCGACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGC  >  NC_000913/429405‑429701
                                                                                                                                                      |                                                                                                                                                   
gCGACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTa                                                                                                                                                     >  1:654085/1‑150 (MQ=255)
    cGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGAc                                                                                                                                                 <  1:1510232/150‑1 (MQ=255)
    cGTAGATGTTTACAGAGATTGTCGGTAAGCGTGCCATCGTAAACCTGCTATATGGAGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGTCACAGGAATATACTGTTGAACAACTAAACCCCGGACGTAAAGTCTATGAc                                                                                                                                                 >  2:439814/1‑150 (MQ=255)
     ggcGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACt                                                                                                                                                <  1:821571/148‑1 (MQ=255)
       cGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACttt                                                                                                                                              <  2:1589588/150‑1 (MQ=255)
       cGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTATATGACttt                                                                                                                                              >  1:1807830/1‑150 (MQ=255)
        gATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTa                                                                                                                                             >  1:1539951/1‑150 (MQ=255)
        gATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTa                                                                                                                                             >  2:49904/1‑150 (MQ=255)
         aTGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTAt                                                                                                                                            >  2:143576/1‑150 (MQ=255)
           gTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCATAGACTTTacgg                                                                                                                                          <  2:1946287/150‑4 (MQ=255)
           gTTTACCGAGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCATAGACTTTacgg                                                                                                                                          <  2:538078/150‑4 (MQ=255)
            tttACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCATAGACTTTacggc                                                                                                                                         <  1:1907648/150‑5 (MQ=255)
             ttACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATgcgc                                                                                                                                        >  2:744351/1‑150 (MQ=255)
              tACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCt                                                                                                                                       >  1:904267/1‑150 (MQ=255)
                ccGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGCAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGAGCTgg                                                                                                                                     >  1:325879/1‑150 (MQ=255)
                ccGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTgg                                                                                                                                     <  2:239494/150‑1 (MQ=255)
                ccGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTgg                                                                                                                                     <  2:214729/150‑1 (MQ=255)
                ccGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTgg                                                                                                                                     >  2:1335933/1‑150 (MQ=255)
                ccGCGAATGTCGGTACGCGTGCCATCGTAAAACTCCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGACGAGTGCGATGTGGCACAGGAAAATACTGTTGAACAACTAAAACACGGCCGTAAAGTCTATGACTTTATGAGCTgg                                                                                                                                     >  1:342042/1‑150 (MQ=255)
                   cgATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGac                                                                                                                                  <  2:480066/150‑1 (MQ=255)
                   cgATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGac                                                                                                                                  <  1:1488597/150‑1 (MQ=255)
                    gATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATTTACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATCACTGGATGCGCTGGGact                                                                                                                                 >  1:1143924/1‑150 (MQ=255)
                        gTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCTAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTg                                                                                                                             >  1:72555/1‑150 (MQ=255)
                        gTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTg                                                                                                                             >  1:1372735/1‑150 (MQ=255)
                        gTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTg                                                                                                                             >  1:1104804/1‑150 (MQ=255)
                           ggTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTCCTGGGc                                                                                                                          >  1:386696/1‑150 (MQ=255)
                            gTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGATCAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCt                                                                                                                         >  2:426774/1‑150 (MQ=255)
                               cgcgTGCCTTCTTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAATCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTc                                                                                                                      <  2:124532/150‑1 (MQ=255)
                                 cgTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCgg                                                                                                                    >  2:743241/1‑150 (MQ=255)
                                        tCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATctct                                                                                                             >  1:1106453/1‑150 (MQ=255)
                                           tAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGt                                                                                                          >  2:578680/1‑150 (MQ=255)
                                            aaaCCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTc                                                                                                         >  2:1670000/1‑150 (MQ=255)
                                              aCCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACACCTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTctg                                                                                                       >  1:1052457/1‑150 (MQ=255)
                                              aCCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTctg                                                                                                       >  1:1012408/1‑150 (MQ=255)
                                                 tGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTctgctg                                                                                                    <  1:1326680/150‑1 (MQ=255)
                                                 tGCTATATGGCGGCAAGCGAGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGGTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATTCGATGGGTCTACTGGGCTTTCGGCATCTCTGGTctgctg                                                                                                    >  1:279390/1‑150 (MQ=255)
                                                  gCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGt                                                                                                   <  2:562464/150‑1 (MQ=255)
                                                  gCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGt                                                                                                   >  2:1852680/1‑150 (MQ=255)
                                                   cTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCATAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGtt                                                                                                  <  1:1397445/150‑1 (MQ=255)
                                                   cTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGGGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGtt                                                                                                  <  1:525262/150‑1 (MQ=255)
                                                       atGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGTAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATc                                                                                              <  1:1598397/150‑1 (MQ=255)
                                                       atGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTTTGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATc                                                                                              <  1:957090/150‑1 (MQ=255)
                                                        tGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCg                                                                                             >  2:265674/1‑150 (MQ=255)
                                                         ggcggcACGCGCGTCAAGAATCTGTCAAGCTGAGGAGTGAGATGTGTAACAGGAATATAATGTTTAACAAATAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTTCTGGGCTTTCGTCATCTCTGGTCTGCTGTTAATCgc                                                                                            <  2:324669/150‑1 (MQ=255)
                                                         ggcggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgc                                                                                            >  2:63324/1‑150 (MQ=255)
                                                         ggcggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgc                                                                                            <  2:1950672/150‑1 (MQ=255)
                                                         ggcggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgc                                                                                            >  2:69334/1‑150 (MQ=255)
                                                          gcggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgct                                                                                           >  1:1643712/1‑150 (MQ=255)
                                                           cggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAAGATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTTATCgcgg                                                                                          >  1:371807/1‑148 (MQ=255)
                                                           cggcAAGCGCGGCAAGAAGCTGGCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgctg                                                                                          <  1:1313287/150‑1 (MQ=255)
                                                            ggcAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCgctgc                                                                                         <  1:869590/150‑1 (MQ=255)
                                                                     gTCAAGAAGCTGTCAATCTGAGGAGTGCGATGAGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGttat                                                                                >  1:1396634/1‑150 (MQ=255)
                                                                        aagaagCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTtattat                                                                             <  1:564397/150‑1 (MQ=255)
                                                                         agaagCTTTCAATCTGAGTAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCTGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATg                                                                            <  1:1743510/150‑1 (MQ=255)
                                                                         agaagCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATg                                                                            <  2:823346/150‑1 (MQ=255)
                                                                         agaagCTGTCAAGCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATg                                                                            <  1:1056001/150‑1 (MQ=255)
                                                                             gCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCg                                                                        >  1:150499/1‑150 (MQ=255)
                                                                              cTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGACATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGt                                                                       >  1:279569/1‑150 (MQ=255)
                                                                                  cAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTgcgc                                                                   >  2:896698/1‑150 (MQ=255)
                                                                                  cAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTgcgc                                                                   >  1:1481539/1‑150 (MQ=255)
                                                                                     tCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAATCCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGc                                                                <  1:1459548/150‑1 (MQ=255)
                                                                                        gaggagTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCttt                                                             >  2:75168/1‑150 (MQ=255)
                                                                                        gaggagTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCttt                                                             >  1:1293545/1‑150 (MQ=255)
                                                                                         aggagTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTa                                                            <  2:686075/150‑1 (MQ=255)
                                                                                            tgTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACt                                                         <  2:763616/149‑1 (MQ=255)
                                                                                            agTGCGATGTGGCACAGGAATATACTGTTGAAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAAc                                                          <  1:714250/150‑1 (MQ=255)
                                                                                            agTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTTAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACt                                                         <  1:143576/150‑1 (MQ=255)
                                                                                              tGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTgg                                                       >  2:1862031/1‑150 (MQ=255)
                                                                                                cGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTgggg                                                     >  1:406140/1‑150 (MQ=255)
                                                                                                  aTGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGc                                                   >  2:137056/1‑150 (MQ=255)
                                                                                                     tgGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGGCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTgg                                                <  2:925424/150‑1 (MQ=255)
                                                                                                       gCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGAt                                              >  2:841008/1‑150 (MQ=255)
                                                                                                        cacaGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGAtt                                             >  2:1803274/1‑150 (MQ=255)
                                                                                                           aGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCa                                          >  1:142018/1‑150 (MQ=255)
                                                                                                            ggAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAc                                         >  2:1477992/1‑150 (MQ=255)
                                                                                                             gAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTGCGGCATCTCTGGGCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCAcc                                        <  2:1774168/150‑1 (MQ=255)
                                                                                                                 ataCTGTTGAACAACTAAACCACGGCCGTACAGGCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGGTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCggtg                                    <  2:891383/150‑1 (MQ=255)
                                                                                                                 ataCTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCggtg                                    <  1:557618/150‑1 (MQ=255)
                                                                                                                  taCTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGCTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCggtgg                                   <  2:933913/150‑1 (MQ=255)
                                                                                                                    cTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTa                                 >  1:1911294/1‑150 (MQ=255)
                                                                                                                       ttGAACAACTAAACCACGGCCGTAAAGTCTCTGACTTTATGCGCGGGGACTACTGGGCTTTCGGCTTCTCTGGTCTGCTGTTAATCGCTGCTATCGTTTTTATGGGCGTGCGCGGCTTTAACTGGGGGCGGGATTTCACCGGTGGTACgg                              <  1:159101/150‑1 (MQ=255)
                                                                                                                       ttGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACgg                              <  2:1372735/150‑1 (MQ=255)
                                                                                                                       ttGAACAACTAAACCACGGCCCTACAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGGATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACgg                              <  2:1490578/150‑1 (MQ=255)
                                                                                                                        tGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGt                             >  2:794314/1‑150 (MQ=255)
                                                                                                                        tGAACAACTAAACCACGGCCGTAAAGGCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTAATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGt                             <  2:169402/150‑1 (MQ=255)
                                                                                                                         gAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGtt                            >  2:1640527/1‑150 (MQ=255)
                                                                                                                           caacaatAACCCACGCCCGTCAAGTCTCTCTCTTAATGCGCGTGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAAGCGCTGCTATCGTTTTTATGGGCGGGCGCGGCTTTAACTGGGGGGTTCATTTCCCCGGTGGTACGGTTa                           <  2:329584/144‑1 (MQ=255)
                                                                                                                           aacaacTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTa                           >  2:1016090/1‑150 (MQ=255)
                                                                                                                            acaacTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTAt                          <  2:1552609/150‑1 (MQ=255)
                                                                                                                             caacTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTAtt                         >  2:1066210/1‑150 (MQ=255)
                                                                                                                             caacTAAACCACGGCCGTAAAGGCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTAtt                         <  2:1440232/150‑1 (MQ=255)
                                                                                                                               acTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGa                       >  1:185935/1‑150 (MQ=255)
                                                                                                                                  aaaCCACGGCCGTAACCTCTATCACTTTATGCGCTGGGACTACTGGGCTTGCGGCAGCTCTGGGCTGCTGTGAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAAt                    <  2:325879/150‑1 (MQ=255)
                                                                                                                                    aCCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTa                  <  1:49904/150‑1 (MQ=255)
                                                                                                                                      cACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACg                >  2:1657967/1‑150 (MQ=255)
                                                                                                                                         gcgcatAAAGTCATAGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTTACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTc             >  1:1281034/6‑150 (MQ=255)
                                                                                                                                          gCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCg            >  1:1669771/1‑150 (MQ=255)
                                                                                                                                          cgcatAAAGTCATAGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTCGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCg            >  2:1271302/5‑150 (MQ=255)
                                                                                                                                           gcatAAAGTCATAGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGa           >  1:533800/4‑150 (MQ=255)
                                                                                                                                                   tCTATGACTTTATGAGCTGGGACTACTGGGCTTTAGGCATCTCTGGTCTGCTGTTAATAGCTTCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGTGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCgg   <  1:827813/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GCGACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGC  >  NC_000913/429405‑429701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: