Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,283,232 A→C 5.7% intergenic (‑157/+21) yjcF ← / ← actP pentapeptide repeats protein/acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,283,2320AC 5.7% 303.1 / 6.4 122intergenic (‑157/+21)yjcF/actPpentapeptide repeats protein/acetate transporter
Reads supporting (aligned to +/- strand):  ref base A (57/57);  new base C (2/6);  total (59/63)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

Only 100 of 126 total aligned reads displayed.

CTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGA  >  NC_000913/4283084‑4283380
                                                                                                                                                    |                                                                                                                                                    
cTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAgac                                                                                                                                                     <  2:1147565/150‑1 (MQ=255)
        tAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCGTCTGACCgggg                                                                                                                                             <  2:134305/150‑1 (MQ=255)
         aaCAAGAAAGAAAAGGCCTATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCGTCTGACCGGGGa                                                                                                                                            <  1:1057977/150‑1 (MQ=255)
           cAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATGGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCGTCTGACCGGGGaat                                                                                                                                          <  2:223911/150‑2 (MQ=255)
           cAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCATACGACCGGGGAAg                                                                                                                                          >  1:1786915/1‑150 (MQ=255)
           cAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAg                                                                                                                                          >  2:1037066/1‑150 (MQ=255)
           cAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAg                                                                                                                                          >  2:1583702/1‑150 (MQ=255)
            aagaaagaAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGGGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAgg                                                                                                                                         <  1:1718644/150‑1 (MQ=255)
             agaaagaaAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAggg                                                                                                                                        >  1:1351563/1‑150 (MQ=255)
             agaaagaaAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAggg                                                                                                                                        >  2:1318478/1‑150 (MQ=255)
             agaaagaaAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAggg                                                                                                                                        <  2:1920272/150‑1 (MQ=255)
              taaagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTAATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAgggg                                                                                                                                       <  2:1134189/149‑1 (MQ=255)
              gaaagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAgggg                                                                                                                                       <  2:1426340/150‑1 (MQ=255)
              gaaagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAgggg                                                                                                                                       <  2:133868/150‑1 (MQ=255)
              gaaagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCAGTTTTTAATGAGTTTATTTGTTTAATATTATGGGTAAAGGTGATTAATTTGGGAGATGAAGAGTATTCCCCCGTAAGACGACCGGGGAAgggg                                                                                                                                       >  1:1631807/1‑150 (MQ=255)
               aaagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGga                                                                                                                                      >  2:1593201/1‑150 (MQ=255)
                aagaaaAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGgag                                                                                                                                     <  2:1552996/150‑1 (MQ=255)
                aagaaaAGGAATAATCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGCGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGgag                                                                                                                                     <  2:1561115/150‑1 (MQ=255)
                  caaaaGCCAAATTCTCTGCAATATCATTTTCTACGACTCTCAAAAATCGCCCATTTTTTAATGAGATTATTTGATAAATATTATGGGAAAAGGGGAGGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGgagag                                                                                                                                   <  1:378044/149‑1 (MQ=255)
                       ggCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTaa                                                                                                                              >  2:1158912/1‑150 (MQ=255)
                       ggCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCCGTAAGAAGACCGGGGAAGGGGATAGATTaa                                                                                                                              >  1:850529/1‑150 (MQ=255)
                            atTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATgcgc                                                                                                                         >  1:1332640/1‑150 (MQ=255)
                             ttCTCTGCTTTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATgcgcg                                                                                                                        <  2:918002/150‑1 (MQ=255)
                                tctGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATTCGCGCgg                                                                                                                     >  1:873196/1‑150 (MQ=255)
                                 ctGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGc                                                                                                                    <  1:511491/150‑1 (MQ=255)
                                      ttATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGc                                                                                                               <  1:90305/150‑1 (MQ=255)
                                       tATCATTTTCTACGACTGTCAAAAATCGCTCAATTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCt                                                                                                              <  2:365766/150‑1 (MQ=255)
                                          cATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCCGTAAGACCACCGGGGCAGGGGTGAGATTTATACGCGCGGCATTGCTcca                                                                                                           >  2:1825712/1‑148 (MQ=255)
                                            ttttCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATTTTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACg                                                                                                         <  1:377387/150‑1 (MQ=255)
                                               tCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGTAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCa                                                                                                      <  1:1399545/150‑1 (MQ=255)
                                                cTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTAAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCaa                                                                                                     <  2:1239194/150‑1 (MQ=255)
                                                  aCGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAg                                                                                                   >  2:1546094/1‑150 (MQ=255)
                                                     aCTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATTTTTTGGGTAATGGTGAGGCATTTGGGAGAGGAAGAGTATTCCCCGGGCAGCCGCCCGGGGATGGGGAGAGTTTAATGGGCGCGGCCTTTCTCAACGCCAAAGCCg                                                                                                >  2:771459/1‑150 (MQ=255)
                                                      cTGTCAAACATCTATCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCgg                                                                                               <  2:1114879/150‑1 (MQ=255)
                                                        gTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCTGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGTTc                                                                                             >  1:1816269/1‑150 (MQ=255)
                                                        gTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTACACGGTAATACGAACGGGGAAGGGGAGAGATTAATGAGCGCGGCCTTGCTCAACGCCAAAGCCGGtg                                                                                             >  1:269989/1‑149 (MQ=255)
                                                          cAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTg                                                                                           <  1:1583702/150‑1 (MQ=255)
                                                               aTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGc                                                                                      <  2:803655/150‑1 (MQ=255)
                                                                 cGCTCATTTTTTAATGAGTTTATTTTTTTAATATTTTGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGGCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCgg                                                                                    <  2:1824721/150‑1 (MQ=255)
                                                                 cGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGTAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCgg                                                                                    >  2:1529753/1‑150 (MQ=255)
                                                                 cGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCgg                                                                                    <  2:36318/150‑1 (MQ=255)
                                                                 cGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCgg                                                                                    >  2:513097/1‑150 (MQ=255)
                                                                 cGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAAGACCGGGGAAGGGGAGAGAATAATGCGCGCCGCCTTGCTCAAAGCCAAAGCCGGTCTGGGAGCgg                                                                                    >  2:570186/1‑150 (MQ=255)
                                                                  gCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCCGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCCGCCTTTCTCAACCCCAAAGCCGGTATGGGAGCGGa                                                                                   >  2:1777827/1‑150 (MQ=255)
                                                                     cATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATTCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATaa                                                                                >  1:1463653/1‑150 (MQ=255)
                                                                     cATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCCGTCAGACCCCCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCACAACCCCAAAGCCGGTCTGGGAGCGGATaa                                                                                >  2:400669/1‑150 (MQ=255)
                                                                     cATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCACACGTCAGACAACCGGGGAAGGGGAGAGATTAATGCGCGCGTCCTTGCTCAACCCCAAAGCCGGTCTGGGAGCGGATaa                                                                                >  1:1967768/1‑150 (MQ=255)
                                                                     aaTTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATaa                                                                                <  2:488231/149‑1 (MQ=255)
                                                                      cttttttAATTAGTTTCTTTGTGTAATATTAGGGGCAACGGTGATGCCTTTGGGAGAGGACGCGTATTCCCCGGGCAGACGTCCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCCACCCCAAAGCCGGTCTGGGAGCGGATaaa                                                                               <  1:354913/149‑1 (MQ=255)
                                                                      aTTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGCCGACCGGGGAAGGGGAGAGATTAATGAGCGCGGCCTTGCTAAAAGCCAAAGCCGGTATGGGAGCGGATaaa                                                                               >  2:1010329/1‑150 (MQ=255)
                                                                         ttttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTg                                                                            <  2:2008546/150‑1 (MQ=255)
                                                                         ttttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATACCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTg                                                                            <  1:922433/150‑1 (MQ=255)
                                                                          tttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTgc                                                                           <  2:1568578/150‑1 (MQ=255)
                                                                              aTGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTgcgcgc                                                                       >  1:74474/1‑150 (MQ=255)
                                                                               tGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGcgcgcg                                                                      <  2:671582/150‑1 (MQ=255)
                                                                                 aGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGa                                                                    >  2:509913/1‑150 (MQ=255)
                                                                                 aGTTTATTTGATAAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGa                                                                    <  2:678317/150‑1 (MQ=255)
                                                                                  gTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGATAGATTAATGCGCGCGGCCTTGTTCAACCCCAAAGCCGGTCTGGGAGCGGATAAAATGCGCGCGGaa                                                                   >  2:1780716/1‑150 (MQ=255)
                                                                                   tttATTTTTTTAATATTATGGTAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAAc                                                                  <  1:2018247/150‑1 (MQ=255)
                                                                                    ttATTTGTTTAATATTATGGGAAAAGGTGATGCCTTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACa                                                                 <  2:321782/150‑1 (MQ=255)
                                                                                     tATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACat                                                                >  2:651594/1‑149 (MQ=255)
                                                                                         tGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtca                                                            <  2:502706/150‑1 (MQ=255)
                                                                                         tGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATCAGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGttaa                                                            >  2:286823/1‑148 (MQ=255)
                                                                                          gTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcac                                                           <  2:880911/150‑1 (MQ=255)
                                                                                          gTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCCGTCAGACCACCGGGGAAGGGGAGAGATTAATTCGCGCGGCCAAGCTCAACGCCAAAGCCCGTCTTGGAGCGGAATAAAAGCGCGCCGAACAGTtaac                                                           >  2:1273303/1‑150 (MQ=255)
                                                                                            ttAATATTATGGGAAAAGGTGATGCATTTGGGAGAAGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgc                                                         >  1:1890553/1‑150 (MQ=255)
                                                                                              aaTATTATGGGAACAGGGGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGGCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctc                                                       <  1:1056830/150‑1 (MQ=255)
                                                                                                tattatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctcac                                                     >  1:106996/1‑150 (MQ=255)
                                                                                                 attatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctcacg                                                    <  2:1285729/150‑1 (MQ=255)
                                                                                                      gggAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTTCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACgcgcgc                                               >  1:789841/1‑150 (MQ=255)
                                                                                                          aaaGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTc                                           >  2:75694/1‑150 (MQ=255)
                                                                                                           aaGGTGATGCATTTGGGAGAGGAAGAGAATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTcc                                          <  2:91068/150‑1 (MQ=255)
                                                                                                             ggTGATGCATTTGGGAGAGGAAGAGTATTCCCCCGTCAGACGACCCGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGc                                        >  1:577422/1‑150 (MQ=255)
                                                                                                              gTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCt                                       <  1:1158912/150‑1 (MQ=255)
                                                                                                                 aTGCATTTGGGAGAGGAAGTGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAg                                    <  1:88373/150‑1 (MQ=255)
                                                                                                                 aTGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAg                                    <  2:1972996/150‑1 (MQ=255)
                                                                                                                    cATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGAGCGGCCTTGCTCAACGCCAAAGCAGGTCTGGGAGAGGATATACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTGCGCTGAGTTa                                 >  1:1029608/1‑150 (MQ=255)
                                                                                                                        tGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTTATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATTAACTGCGCGCGGAACAGTTCCCGCTCACGCGCGCCTTCCGCTGAGTTATCgg                             >  1:1628170/1‑150 (MQ=255)
                                                                                                                              agGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCg                       <  2:1332640/150‑1 (MQ=255)
                                                                                                                                gAAGCGTATTCCCCGGTCAGCCGACCGGGGAAGGGGAGAGATTAATGCGGGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCgag                     <  1:364954/150‑1 (MQ=255)
                                                                                                                                 aaGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCgaga                    <  1:1115573/150‑1 (MQ=255)
                                                                                                                                 aaGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTACTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCgaga                    >  2:1008643/1‑150 (MQ=255)
                                                                                                                                  agagTATTCCCCGGTCAGACGGCCGGGGAAGGGGAGCGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCCCGCGCGCCTTCCGCTGAGGTATCGGTTGCCGagaa                   >  1:1173928/1‑150 (MQ=255)
                                                                                                                                     ttATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAgaagaa                <  1:1777827/149‑1 (MQ=255)
                                                                                                                                     gTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAgaagaa                >  1:1687701/1‑150 (MQ=255)
                                                                                                                                       aTTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAcc              >  2:1978010/1‑150 (MQ=255)
                                                                                                                                       aTTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAcc              >  1:775595/1‑150 (MQ=255)
                                                                                                                                        ttCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCCCGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAccc             >  1:1618580/1‑149 (MQ=255)
                                                                                                                                        ttCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGACCCa             >  1:230218/1‑150 (MQ=255)
                                                                                                                                        ttCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCa             <  1:237801/150‑1 (MQ=255)
                                                                                                                                        ttCCCCGGCCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTGGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCa             <  1:1180304/150‑1 (MQ=255)
                                                                                                                                         tCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAg            >  1:884567/1‑150 (MQ=255)
                                                                                                                                         tCCCCGGGCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGGCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAg            <  1:1318478/150‑1 (MQ=255)
                                                                                                                                           cccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGAt          >  1:1281596/1‑150 (MQ=255)
                                                                                                                                               gTCAGACGACCGGGGAAGGGGAGAGATTAAAGCGAGAGGCCTTGCTCAACGCCAACTCCGGGCTGGGAGCTGATAAACTGCGCGCGGACGAGTTCACGCTCACGCGCGCCTGCGGATGAGTTATCGGTTGCCGAGAAGCACCAGATGCCg      >  2:1259174/1‑150 (MQ=255)
                                                                                                                                                gcAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGa     <  1:1546094/149‑1 (MQ=255)
                                                                                                                                                 cAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAg    <  1:503125/150‑1 (MQ=255)
                                                                                                                                                  aGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAgg   <  2:269989/150‑1 (MQ=255)
                                                                                                                                                   gacgacCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGa  >  1:1493410/1‑150 (MQ=255)
                                                                                                                                                   gacgacCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGa  >  2:839593/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGGA  >  NC_000913/4283084‑4283380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: