Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,312,083 G→C 6.0% intergenic (‑41/+18) alsB ← / ← alsR D‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,0830GC 6.0% 413.9 / 13.5 133intergenic (‑41/+18)alsB/alsRD‑allose ABC transporter periplasmic binding protein/d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
Reads supporting (aligned to +/- strand):  ref base G (49/76);  new base C (4/4);  total (53/80)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 135 total aligned reads displayed.

GGTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTC  >  NC_000913/4311934‑4312231
                                                                                                                                                     |                                                                                                                                                    
ggTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATg                                                                                                                                                      <  1:584367/150‑1 (MQ=255)
ggTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATg                                                                                                                                                      >  1:1588394/1‑150 (MQ=255)
  ttGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGc                                                                                                                                                    <  2:147563/150‑1 (MQ=255)
    ggAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCgg                                                                                                                                                  <  2:506521/150‑1 (MQ=255)
    ggAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCGGGTTGACAACATTAAGCCCCCGAGTGTGCCGCTGAACTATTTCAGATATTTATTCATAATAATGTTCTCCCGGTAAGGGGACAGGTATCTCCGCGCTGGCgg                                                                                                                                                  >  2:1432021/1‑150 (MQ=255)
     gagaGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGa                                                                                                                                                 <  1:17990/150‑1 (MQ=255)
      agagGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACCACATTAAGCCCACGAGTGTGCCGCGGCAATCTTTCAGATATTTATTCATAATAATGTACTCACTGTAAGGGGACAGGTAACTCCGCGATCGCGGaa                                                                                                                                                >  1:1434253/1‑150 (MQ=255)
      agagGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGaa                                                                                                                                                >  1:194040/1‑150 (MQ=255)
       gagGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAAt                                                                                                                                               <  1:415284/150‑1 (MQ=255)
         gggTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTa                                                                                                                                             >  1:1119317/1‑150 (MQ=255)
         gggTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTa                                                                                                                                             >  1:1989043/1‑150 (MQ=255)
         gggTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTa                                                                                                                                             <  1:1605003/150‑1 (MQ=255)
           gTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                                                                           <  1:1867811/150‑1 (MQ=255)
           gTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTAcc                                                                                                                                           <  2:496815/150‑1 (MQ=255)
             tttCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCtt                                                                                                                                         >  1:1847876/1‑150 (MQ=255)
               tCACTACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAATTCCGCCATCGCGGAGTTACCtgtc                                                                                                                                       <  2:1289298/150‑4 (MQ=255)
               tCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCtttt                                                                                                                                       <  1:1857544/150‑1 (MQ=255)
                 aaTACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTCTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTc                                                                                                                                     <  2:1166380/150‑1 (MQ=255)
                  aTACGACAGCATATTCGGCGTCATCAAAAGCGCTTGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGAAATTTATTCATAATAATGATCTCACGGACAGGGGACAGGTAACTCCGCGAGGGCGGAATTACCTTTTTCt                                                                                                                                    <  1:1343832/150‑1 (MQ=255)
                  aTACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCt                                                                                                                                    <  1:375846/150‑1 (MQ=255)
                   tACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCtt                                                                                                                                   >  1:1496539/1‑150 (MQ=255)
                      caCAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGGTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAtt                                                                                                                                <  1:1783585/149‑1 (MQ=255)
                        cAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTc                                                                                                                              <  2:460249/150‑1 (MQ=255)
                          gCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAg                                                                                                                            <  2:1847876/150‑1 (MQ=255)
                           cATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgc                                                                                                                           >  2:265605/1‑150 (MQ=255)
                            atatTCGGCGGCAGCAAACGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcg                                                                                                                          >  1:51794/1‑150 (MQ=255)
                            atatTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcg                                                                                                                          <  1:1151387/150‑1 (MQ=255)
                               ttCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcgc                                                                                                                       <  1:488449/150‑1 (MQ=255)
                               ttCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAgcgcgc                                                                                                                       >  1:544492/1‑150 (MQ=255)
                                tcgtcgGCAGCAAATGCGCTGGTTGACAACATAAAGCCCTCGAGTGTGACGCTGAAATAATTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGcc                                                                                                                      <  2:1587287/150‑1 (MQ=255)
                                tCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGcc                                                                                                                      <  1:1517008/150‑1 (MQ=255)
                                   gctgcatcaATAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTgg                                                                                                                   <  1:1090373/147‑1 (MQ=255)
                                      gcagcaTAAGCGCTGGTTGACTACATTAAGCCCTCGAGTGTGCCGGTGATATATTTCAGATATTTAATCATATTATTGTTCTGACGGTAAGGGGTCAGGTTAGTCCGCTATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTga                                                                                                                <  2:1435092/150‑1 (MQ=255)
                                      gcagcaAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTga                                                                                                                <  2:357406/150‑1 (MQ=255)
                                       cagcaAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATATCAGATATTTATTCAAAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGATTTACCTTTTTCTTATTTCAGCTCGCCTGGTgag                                                                                                               >  1:1996337/1‑150 (MQ=255)
                                         gcaAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTTACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGagaa                                                                                                             <  1:415365/150‑1 (MQ=255)
                                           aaaaGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTATTTCTTATTTCAGCGCGCCTGGTGagaaga                                                                                                           >  1:724183/1‑150 (MQ=255)
                                            aaaGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAgaagaa                                                                                                          <  2:193563/150‑1 (MQ=255)
                                            aaaGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATATATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCAGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAgaagaa                                                                                                          >  2:354362/1‑150 (MQ=255)
                                               gcgcTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATc                                                                                                       >  2:293279/1‑150 (MQ=255)
                                                cgcTGGTTTACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCTGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATca                                                                                                      <  1:537341/150‑1 (MQ=255)
                                                cgcTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATca                                                                                                      >  2:706735/1‑150 (MQ=255)
                                                   tGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATcaaca                                                                                                   <  2:779751/150‑1 (MQ=255)
                                                      ttGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTATCTCGGTAAGGGGACAGGTAACTCCTCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAAtt                                                                                                >  1:1265591/1‑150 (MQ=255)
                                                       tGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTg                                                                                               <  2:825203/150‑1 (MQ=255)
                                                       tGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTAGTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTg                                                                                               >  2:1808865/1‑150 (MQ=255)
                                                       tGACAACATTAAGCCCACGAGTGTGCCACTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTg                                                                                               >  2:1966797/1‑150 (MQ=255)
                                                         acaacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTgcg                                                                                             <  2:557596/150‑1 (MQ=255)
                                                          caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCCGCGCGCCTGGTGAGAAGAAATCAACAATTgcgc                                                                                            >  2:960211/1‑150 (MQ=255)
                                                          caacaTTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAAACAACAATTgcgc                                                                                            >  1:518546/1‑150 (MQ=255)
                                                              aTTAAGCCCACGAGTGTGCAGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGt                                                                                        <  1:1435254/150‑1 (MQ=255)
                                                               ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtt                                                                                       <  2:1840748/150‑1 (MQ=255)
                                                               ttAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtt                                                                                       <  1:744925/150‑1 (MQ=255)
                                                                   tcccACGAGTGTGTCGCTGAAATATTTCATTTATTTATTCATAATAATGTTCTCACGGTAAGGTGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtttttt                                                                                   <  1:391354/149‑1 (MQ=255)
                                                                   gCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGtttttt                                                                                   >  2:587116/1‑150 (MQ=255)
                                                                    cccACGATTGTGCCGCTGAAATATTTCAGATATTTATTCATAAAAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                                                                  <  2:1006759/150‑1 (MQ=255)
                                                                    cccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                                                                  <  2:1119317/150‑1 (MQ=255)
                                                                    cccACGAGGGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTg                                                                                  <  2:1435644/150‑1 (MQ=255)
                                                                     ccACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGc                                                                                 <  2:1226485/150‑1 (MQ=255)
                                                                          aGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatatt                                                                            <  1:1546381/150‑1 (MQ=255)
                                                                          aGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGGAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatatt                                                                            <  1:395280/150‑1 (MQ=255)
                                                                                ccGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatatt                                                                      >  2:671713/1‑150 (MQ=255)
                                                                                ccGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCatattaatatt                                                                      <  1:863435/150‑1 (MQ=255)
                                                                                    tGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTCGCATATTAATATTAGCt                                                                  <  2:1358218/150‑1 (MQ=255)
                                                                                       aaTATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGt                                                               >  2:388304/1‑150 (MQ=255)
                                                                                         tatTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTc                                                             <  2:2016419/150‑1 (MQ=255)
                                                                                           tttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCaa                                                           >  2:1343278/1‑150 (MQ=255)
                                                                                           tttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCaa                                                           >  1:1778611/1‑150 (MQ=255)
                                                                                            ttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGCCAGGTATCTCCGCGATGGAGGAATTTCCTTTTTCTTTTTTCATCTCGTCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAAt                                                          <  1:324470/150‑1 (MQ=255)
                                                                                            ttCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAAt                                                          <  2:692753/150‑1 (MQ=255)
                                                                                                gATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTg                                                      <  1:1276527/150‑1 (MQ=255)
                                                                                                 atatTTATTCATAATAATGTTCTCACTGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCTCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGa                                                     <  1:230034/150‑1 (MQ=255)
                                                                                                   atttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGc                                                   >  2:548662/1‑150 (MQ=255)
                                                                                                     ttattCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTg                                                 >  1:1959621/1‑150 (MQ=255)
                                                                                                        ttCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGTCAGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACATTTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTGAATGTTGAGCTGGGc                                              <  1:354362/150‑1 (MQ=255)
                                                                                                        ttCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGc                                              >  1:108766/1‑150 (MQ=255)
                                                                                                          cagaataatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGCTGGCGGATTTACCTTTTTCTTATTTCATCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGa                                            >  1:1421283/4‑150 (MQ=255)
                                                                                                            taataaTGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACa                                          >  1:1727924/1‑150 (MQ=255)
                                                                                                            taataaTGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACa                                          <  1:932508/150‑1 (MQ=255)
                                                                                                              ataatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTTTTCAATGTTGATCTGGTCTACaga                                        >  1:962212/1‑150 (MQ=255)
                                                                                                              ataatGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACaga                                        <  2:665403/150‑1 (MQ=255)
                                                                                                                     tctcACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACaaaa                                 >  1:16832/1‑150 (MQ=255)
                                                                                                                         aCGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAg                             <  1:1966797/150‑1 (MQ=255)
                                                                                                                           ggTACGGTGACCGGGTACTCCGCGATGGCGGAATTACCTTTTTCTAATTACAGCGCTCCTTGGGAGAAGAAATCAACAATTGCGCCTGTTGTTTGCATATAAAAATTAGCATCTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCg                           <  2:96434/150‑1 (MQ=255)
                                                                                                                           ggTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCg                           <  2:2000476/150‑1 (MQ=255)
                                                                                                                              aaGGGGACAGGTAACTCCGCTATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTcc                        <  2:777867/150‑1 (MQ=255)
                                                                                                                                ggggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAg                      >  2:1957937/1‑150 (MQ=255)
                                                                                                                                  ggACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGCCAGAGACAAAAAACGCGTCAAGCa                    >  2:1014454/1‑150 (MQ=255)
                                                                                                                                     cAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAag                 <  1:293279/150‑1 (MQ=255)
                                                                                                                                     cAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAag                 >  1:1082994/1‑150 (MQ=255)
                                                                                                                                     aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCACCAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCCTCCAGCAAag                 >  2:926477/2‑150 (MQ=255)
                                                                                                                                     aaGGTAATTCCGCCATCGCGGAGTTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAag                 >  2:1225076/2‑150 (MQ=255)
                                                                                                                                       ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAagtt               <  1:926449/150‑1 (MQ=255)
                                                                                                                                       ggTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGATTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAagtt               >  1:1622647/1‑150 (MQ=255)
                                                                                                                                         tAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAagttag             <  2:1989043/150‑1 (MQ=255)
                                                                                                                                          aaCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAagttagt            <  1:765435/150‑1 (MQ=255)
                                                                                                                                           aCTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAagttagtt           >  1:1149305/1‑150 (MQ=255)
                                                                                                                                            cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTg          <  2:1177637/150‑1 (MQ=255)
                                                                                                                                            cTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAGCGTCCAGCAAAGTTAGTTGt         >  2:1582961/1‑150 (MQ=255)
                                                                                                                                                    tGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTc  >  2:858727/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTC  >  NC_000913/4311934‑4312231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: