Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,323,257 A→G 5.2% intergenic (‑27/+79) phnE ← / ← phnD defective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily/phosphonate ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,323,2570AG 5.2% 420.3 / 8.9 135intergenic (‑27/+79)phnE/phnDdefective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily/phosphonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (67/61);  new base G (5/2);  total (72/63)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 138 total aligned reads displayed.

GATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGC  >  NC_000913/4323108‑4323405
                                                                                                                                                     |                                                                                                                                                    
gATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATa                                                                                                                                                      >  1:862855/1‑150 (MQ=255)
 aTCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATaa                                                                                                                                                     >  1:1849655/1‑150 (MQ=255)
   cAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAgg                                                                                                                                                   <  1:1097162/150‑1 (MQ=255)
   cAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAgg                                                                                                                                                   <  2:1469115/150‑1 (MQ=255)
   cAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAgg                                                                                                                                                   <  2:1609579/150‑1 (MQ=255)
     gCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTg                                                                                                                                                 >  1:1107805/1‑150 (MQ=255)
     gCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTg                                                                                                                                                 <  1:1299522/150‑1 (MQ=255)
     gCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTg                                                                                                                                                 <  1:1488451/150‑1 (MQ=255)
      cGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCATAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGc                                                                                                                                                <  2:90723/150‑1 (MQ=255)
      cGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGc                                                                                                                                                >  2:489509/1‑150 (MQ=255)
            cGGGGCCATTTCCGCGCCCTGCCACGAGACGACCCCCACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGACCCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCAc                                                                                                                                          <  1:1895340/150‑1 (MQ=255)
            cGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCAc                                                                                                                                          >  2:34227/1‑150 (MQ=255)
             ggggCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTGCAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCAcc                                                                                                                                         >  2:1931748/1‑150 (MQ=255)
             ggggCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGGGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCAcc                                                                                                                                         <  1:1002049/150‑1 (MQ=255)
              gggCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCg                                                                                                                                        <  1:1841477/150‑1 (MQ=255)
                  cATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATc                                                                                                                                    >  2:1529712/1‑150 (MQ=255)
                   aTTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATGCGGTGCAAGCACCTTATcc                                                                                                                                   <  2:605143/150‑1 (MQ=255)
                    tttCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCg                                                                                                                                  >  2:749194/1‑150 (MQ=255)
                       ccGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCCGCAGCGAGAACCAGCTGCGCTTGGGTGGGGCGCTGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCa                                                                                                                               >  1:287699/1‑150 (MQ=255)
                       ccGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTCGGCCGGATAAGGTGCTTGCACCGCATCCGGCa                                                                                                                               >  1:481144/1‑150 (MQ=255)
                       ccGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGCTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATGCGGTGCAAGCACCTTATCCGGcc                                                                                                                               <  1:104743/150‑2 (MQ=255)
                         gcgcCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGGGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATc                                                                                                                             <  1:1339620/150‑1 (MQ=255)
                            cccTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAAc                                                                                                                          >  2:1106641/1‑150 (MQ=255)
                               tGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGcc                                                                                                                       <  1:1339074/150‑1 (MQ=255)
                                gCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCt                                                                                                                      >  2:1003097/1‑150 (MQ=255)
                                   aCGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGca                                                                                                                   >  1:766024/1‑150 (MQ=255)
                                   aCGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGca                                                                                                                   >  2:1163004/1‑150 (MQ=255)
                                     gagaCGACCAACACGGCGAGTACAACGGCCCAGCGCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCGCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGcaca                                                                                                                 <  1:129129/150‑1 (MQ=255)
                                           acCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCg                                                                                                           >  2:1340082/1‑150 (MQ=255)
                                                   ggCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCGTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGAc                                                                                                   <  2:1208891/150‑1 (MQ=255)
                                                     cGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGc                                                                                                 >  2:608348/1‑150 (MQ=255)
                                                       aGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCg                                                                                               >  1:876803/1‑150 (MQ=255)
                                                        gTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCgg                                                                                              <  2:1843222/150‑1 (MQ=255)
                                                        gTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCgg                                                                                              <  2:622028/150‑1 (MQ=255)
                                                         tACAACGGCCCAGCTCAGAAGCGAGAACCCGCTGCGCGTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCggg                                                                                             <  1:1643836/150‑1 (MQ=255)
                                                          aCAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCgggg                                                                                            <  1:989866/150‑1 (MQ=255)
                                                           cAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCCCCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGc                                                                                           >  2:1447136/1‑150 (MQ=255)
                                                           cAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGc                                                                                           >  1:1452142/1‑150 (MQ=255)
                                                           cAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGc                                                                                           >  2:442853/1‑150 (MQ=255)
                                                           cAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCAGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGc                                                                                           >  1:313172/1‑150 (MQ=255)
                                                           cAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGAGGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCGGCACATTGTCGGCTGCGACGCCGGGGc                                                                                           >  1:699310/1‑150 (MQ=255)
                                                              cGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGGGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCTTCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTc                                                                                        <  2:1908623/150‑1 (MQ=255)
                                                               ggCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGGGGGGCGATGGTGATGGTTTGCATGGTTGGCTCCGGTCTGGAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCt                                                                                       <  1:962781/150‑1 (MQ=255)
                                                                gCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGGGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCtt                                                                                      <  2:1199587/150‑1 (MQ=255)
                                                                 cccAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTa                                                                                     >  1:183292/1‑150 (MQ=255)
                                                                 cccAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTa                                                                                     <  2:1707961/150‑1 (MQ=255)
                                                                 cccAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCAGGGGCGTCTTa                                                                                     >  1:603068/1‑150 (MQ=255)
                                                                 cccAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAAGTGCTTGCACCGCATCCGGCATCAACGCATGCACATTGTCGGATGCGACGCCGGGGCGTCTTa                                                                                     >  1:1557258/1‑150 (MQ=255)
                                                                  ccAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTAt                                                                                    >  1:1601077/1‑150 (MQ=255)
                                                                    aGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGGCGGATGCGACGCCGGGGCGTCTTATcc                                                                                  <  2:274608/150‑1 (MQ=255)
                                                                     gCTCAGAAGCGAGAACCAGCGGCGCGTGGGGGGGGCGATGGTGATGGGTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATAAGACGCCCAGGCGTCGCATCCg                                                                                 <  2:1264674/150‑1 (MQ=255)
                                                                       tCAGAAGCGCGACCCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGAc                                                                               <  1:1326631/150‑1 (MQ=255)
                                                                        cAGAAGCTAGAACCAGCTGCGCTTGGGTGGTGCGATGGTGATCGAGTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACTTTGTCGGATGCGACGCCGGGGCGTCTTATCCGAcc                                                                              <  1:585349/150‑1 (MQ=255)
                                                                        cAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACTCCTGCACATTGTCGGATGCGCCGCCGGGGCGTCTTATCCGAcc                                                                              >  2:325287/1‑150 (MQ=255)
                                                                         aGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCt                                                                             <  2:287699/150‑1 (MQ=255)
                                                                          gAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCTCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTa                                                                            >  1:415914/1‑150 (MQ=255)
                                                                          gAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTa                                                                            >  2:431643/1‑150 (MQ=255)
                                                                            aGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACg                                                                          >  2:936991/1‑150 (MQ=255)
                                                                                agaACCAGCTGCGCGTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGAttt                                                                      <  2:982501/150‑1 (MQ=255)
                                                                                  aaCCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTAc                                                                    <  1:34227/150‑1 (MQ=255)
                                                                                   aCCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACt                                                                   <  2:201685/150‑1 (MQ=255)
                                                                                      aGCTGCGCTTGGGTGGGGCGATTGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCTGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCa                                                                <  2:1621504/150‑1 (MQ=255)
                                                                                               ggggtgggGCGATGGTGATGGGTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTAc                                                       <  1:720064/149‑1 (MQ=255)
                                                                                                 ggtgggGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGCCCTACGATTTACTGCACCGCTTTACTc                                                     >  2:850140/1‑150 (MQ=255)
                                                                                                 ggtgggGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTAcac                                                     >  2:795538/1‑148 (MQ=255)
                                                                                                  gtgggGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCa                                                    >  2:670376/1‑150 (MQ=255)
                                                                                                  gtgggGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCa                                                    >  1:908341/1‑150 (MQ=255)
                                                                                                   tgggGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCAc                                                   >  2:2018526/1‑150 (MQ=255)
                                                                                                    ggggCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCAcc                                                  >  2:1660982/1‑150 (MQ=255)
                                                                                                    ggggCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCAcc                                                  <  2:1633741/150‑1 (MQ=255)
                                                                                                      ggCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGa                                                >  2:868448/1‑150 (MQ=255)
                                                                                                      ggCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTAGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGa                                                >  2:240872/1‑150 (MQ=255)
                                                                                                               gatggtTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATcgc                                       >  2:1979954/1‑150 (MQ=255)
                                                                                                                atggtTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATcgcg                                      >  1:1185620/1‑150 (MQ=255)
                                                                                                                atggtTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATcgcg                                      >  1:1178249/1‑150 (MQ=255)
                                                                                                                   gtTTGCATGGTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTGGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCtt                                   <  1:358838/150‑1 (MQ=255)
                                                                                                                    tttGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGCCCTACGATTTACTGCACCGCTATACTCACCGGACTCATCGAGCttc                                  >  1:522800/1‑149 (MQ=255)
                                                                                                                          tGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCgt                            <  1:210228/150‑1 (MQ=255)
                                                                                                                           gTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCgtt                           >  2:1822759/1‑150 (MQ=255)
                                                                                                                            tttGGCTCCTGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGAGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCTTCGCGCTTAGCGCgttg                          <  2:1403847/150‑1 (MQ=255)
                                                                                                                            tttGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCgttg                          <  1:621523/150‑1 (MQ=255)
                                                                                                                                 cTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAg                     >  2:1709076/1‑150 (MQ=255)
                                                                                                                                 cTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAg                     >  1:1999083/1‑150 (MQ=255)
                                                                                                                                    cGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCTAACTCATCGCGCTTAGCGCGTTGTTCAGGCg                  <  1:299595/150‑1 (MQ=255)
                                                                                                                                    cGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCg                  <  1:771642/150‑1 (MQ=255)
                                                                                                                                     ggTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCgg                 >  2:442947/1‑150 (MQ=255)
                                                                                                                                     ggTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCgg                 >  1:1077205/1‑150 (MQ=255)
                                                                                                                                     ggTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCgg                 >  1:446651/1‑150 (MQ=255)
                                                                                                                                         ttgatgCCGGATGCGGTGCAAGCACCTTATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCa             >  1:736227/6‑150 (MQ=255)
                                                                                                                                         tGTAGGCCGGATGAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCCCATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCCCCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTccc             >  1:247792/1‑149 (MQ=255)
                                                                                                                                          gTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAg            <  1:1340082/150‑1 (MQ=255)
                                                                                                                                          gTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAg            >  2:1447213/1‑150 (MQ=255)
                                                                                                                                            atgCCGGATGCGGTGCAAGCACCTTATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGt          >  2:1430325/3‑150 (MQ=255)
                                                                                                                                            atgCCGGATGCGGTGCAAGCACCTTATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGt          >  2:710142/3‑150 (MQ=255)
                                                                                                                                            aGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGt          <  1:2018526/150‑1 (MQ=255)
                                                                                                                                             ggCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGAATTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTc         >  1:90651/1‑150 (MQ=255)
                                                                                                                                                cGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATc      >  1:876192/1‑150 (MQ=255)
                                                                                                                                                  gATAAGGTGCTTGCACCGCATCCGGCATCACCGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCa    <  1:1058773/150‑1 (MQ=255)
                                                                                                                                                    tAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGc  >  1:44030/1‑150 (MQ=255)
                                                                                                                                                    tAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGc  <  2:1357247/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATCAGCGTAAGCGGGGCCATTTCCGCGCCCTGCCACGAGACGACCAACACGGCGAGTACAACGGCCCAGCTCAGAAGCGAGAACCAGCTGCGCTTGGGTGGGGCGATGGTGATGGTTTGCATGTTTGGCTCCGGTCTGTAGGCCGGATAAGGTGCTTGCACCGCATCCGGCATCAACGCCTGCACATTGTCGGATGCGACGCCGGGGCGTCTTATCCGACCTACGATTTACTGCACCGCTTTACTCACCGAACTCATCGCGCTTAGCGCGTTGTTCAGGCGGTCCAGGTCATCCAGC  >  NC_000913/4323108‑4323405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑