Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,365,132 T→G 6.0% A75A (GCA→GCC) ‡ cutA ← divalent‑cation tolerance protein, copper sensitivity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,365,1320TG 6.0% 443.7 / 15.4 151A75A (GCA→GCC) ‡cutAdivalent‑cation tolerance protein, copper sensitivity
Reads supporting (aligned to +/- strand):  ref base T (74/68);  new base G (5/4);  total (79/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-01

Only 100 of 151 total aligned reads displayed.

CCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAAT  >  NC_000913/4364984‑4365280
                                                                                                                                                    |                                                                                                                                                    
ccGGCAAACCCGGCAGTGCGGCAAAGTAGCAGGACCAGCGTAAAGATGCGTTGAGCCATGAGCGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTg                                                                                                                                                     <  2:283142/150‑1 (MQ=255)
ccGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTg                                                                                                                                                     >  2:198663/1‑150 (MQ=255)
 cGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTTCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGc                                                                                                                                                    >  1:1979539/1‑150 (MQ=255)
 cGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGc                                                                                                                                                    <  1:705231/150‑1 (MQ=255)
    cAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGAGATGGATGATGAGCCTTCAGGCATTCCAGCAGTGCctg                                                                                                                                                 >  2:1542590/1‑150 (MQ=255)
     aaaaaCGTAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCACCGTGTGTAACAGGGAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCctgc                                                                                                                                                <  1:1001277/150‑1 (MQ=255)
        aaCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAACCATGCGTTGAGCCATGAGAGGTAATCTTTGTCTCCGTGTGTAACAGGTATATCGAGAAGTTGCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTgg                                                                                                                                             <  2:836538/150‑1 (MQ=255)
         aCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTgga                                                                                                                                            <  1:1349273/150‑2 (MQ=255)
           ggAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTggaat                                                                                                                                          <  2:573298/150‑4 (MQ=255)
           ggAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGAGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTga                                                                                                                                          >  1:1067565/1‑150 (MQ=255)
               gTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGGGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTggaatgcct                                                                                                                                      <  1:460316/150‑8 (MQ=255)
                  ctgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACgg                                                                                                                                   >  2:249286/1‑150 (MQ=255)
                  ctgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGAAATCTGTGTCTCCGTGTGTAACAGCTAAAACCAGAAGATCCGCGGTATGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTCCTGCTGAGATACgg                                                                                                                                   >  1:1679734/1‑150 (MQ=255)
                    gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTa                                                                                                                                 <  1:1272382/150‑1 (MQ=255)
                    gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTa                                                                                                                                 <  2:1535975/150‑1 (MQ=255)
                     cAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAg                                                                                                                                <  1:16144/150‑1 (MQ=255)
                        aGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGttt                                                                                                                             <  2:77876/150‑1 (MQ=255)
                        aGTAGCAGGAGCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGGCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGCATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGttt                                                                                                                             <  2:1026684/150‑1 (MQ=255)
                             cAGTATCAGAGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCTTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATTATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTaaa                                                                                                                        <  1:1500020/150‑1 (MQ=255)
                              aGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTaaaa                                                                                                                       <  1:630057/150‑1 (MQ=255)
                                   cAGCGTAAAGATGCGTTGATCCATGAGAGGTAATCTGTGTCTCCGGGTGAAACAGGTAAAACCAGAAGTTCCGTGGTTTGATATGGATGATTAGACTTCAGGCATACCACCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAatcatc                                                                                                                  <  1:187230/150‑1 (MQ=255)
                                     gCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTg                                                                                                                >  1:1191561/1‑150 (MQ=255)
                                      cGTACAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGc                                                                                                               <  2:1770436/150‑1 (MQ=255)
                                      cGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGc                                                                                                               >  1:947384/1‑150 (MQ=255)
                                      cGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGc                                                                                                               >  1:1356568/1‑150 (MQ=255)
                                      cGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGc                                                                                                               <  2:1738976/150‑1 (MQ=255)
                                            gATGCGTTTAGCCATGAGAGTTAATCTGTGTCTCCGTGTGTACAATGTAAAACCAGAAGATCCGTTGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCg                                                                                                         <  1:376242/150‑1 (MQ=255)
                                            gATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGCCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTct                                                                                                         >  1:49529/1‑149 (MQ=255)
                                            gATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCg                                                                                                         >  2:1280239/1‑150 (MQ=255)
                                                  ttGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCt                                                                                                   <  2:507/150‑1 (MQ=255)
                                                    gAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTg                                                                                                 >  1:1994484/1‑150 (MQ=255)
                                                     aGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATTATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGc                                                                                                <  1:937834/150‑1 (MQ=255)
                                                        cATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTcc                                                                                             >  1:1890630/1‑150 (MQ=255)
                                                        cATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTcc                                                                                             >  1:1738778/1‑150 (MQ=255)
                                                            agagGTAATCTGAGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATTTGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCt                                                                                         <  2:946847/150‑1 (MQ=255)
                                                              agGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTTAAATCATCTGCACGTCGTATTCTTGGTCCAGCTTa                                                                                       >  2:285915/1‑150 (MQ=255)
                                                               ggTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTAc                                                                                      >  1:656668/1‑150 (MQ=255)
                                                                   aTCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTc                                                                                  >  2:206549/1‑150 (MQ=255)
                                                                    tCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTcc                                                                                 >  2:714658/1‑150 (MQ=255)
                                                                    tCTGTGTCTCCGTGGGTAAACGGTATAACCAGAAGTTCCGGGGTTTGATATGGATGTTGAGACTTCAGGCATTCCAGCTGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTcc                                                                                 <  1:366061/150‑1 (MQ=255)
                                                                       gtgtCTCCGTGTGTAACAGGTAACACCAGAAGTTCCGGGGGTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCGTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGATCCAGCTTACCTTccccg                                                                              >  2:379511/1‑148 (MQ=255)
                                                                       gtgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAg                                                                              <  2:565770/150‑1 (MQ=255)
                                                                       gtgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTGTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAg                                                                              <  2:1958061/150‑1 (MQ=255)
                                                                         gtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTa                                                                            >  2:1478744/1‑150 (MQ=255)
                                                                            tcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATa                                                                         <  2:1908862/150‑1 (MQ=255)
                                                                                 gtgtAACAGGTAAACCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagag                                                                    <  2:862162/150‑1 (MQ=255)
                                                                                 gtgtAACAGGTAAAACCCTAAGTTCCGGGGGTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTCCCTTCCCAGTAATagagag                                                                    <  2:1656035/150‑1 (MQ=255)
                                                                                 gtgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCAAGTAATagagag                                                                    >  2:608434/1‑150 (MQ=255)
                                                                                   gtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagagag                                                                  >  2:1853940/1‑150 (MQ=255)
                                                                                    tAACAGGTAAAACCAGAAGTTCAGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCAGCTGGAGAGATACGGTTGTTTTTAAAAACATCTGCACTTCGTATTCTTGCTCCAGCTAACATTCCCAGTAATAGAGAGAgg                                                                 >  2:93519/1‑150 (MQ=255)
                                                                                     aaCAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGt                                                                >  1:1953423/1‑150 (MQ=255)
                                                                                      aCAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTa                                                               >  2:343536/1‑150 (MQ=255)
                                                                                       cAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAg                                                              >  1:831663/1‑150 (MQ=255)
                                                                                       cAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAg                                                              >  1:226110/1‑150 (MQ=255)
                                                                                       cAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAg                                                              <  2:1583018/150‑1 (MQ=255)
                                                                                         ggTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGATCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAgcg                                                            >  1:1111358/1‑150 (MQ=255)
                                                                                           tAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGcc                                                          <  1:233602/150‑1 (MQ=255)
                                                                                            aaaaCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTTATAGAGAGAGGTAGCGCCg                                                         >  2:121500/1‑150 (MQ=255)
                                                                                            aaaaCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCg                                                         >  2:41704/1‑150 (MQ=255)
                                                                                            aaaaCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCg                                                         >  2:1467062/1‑150 (MQ=255)
                                                                                              aaCCAGAACTTCCTGGGTTTGATATGGATGATGCGACTTCAGTCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAATTCATCTCCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGGAGCGCCggg                                                       <  1:1814867/150‑1 (MQ=255)
                                                                                               aCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                      >  1:1823337/1‑150 (MQ=255)
                                                                                               aCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                      >  1:1169580/1‑150 (MQ=255)
                                                                                               aCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                      >  1:57353/1‑150 (MQ=255)
                                                                                                ccAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGa                                                     >  2:1311465/1‑150 (MQ=255)
                                                                                                ccAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGAAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGAAATAGAGAGAGGTAGCGCAGGGGa                                                     >  2:1370421/1‑150 (MQ=255)
                                                                                                   gAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCa                                                  <  1:934848/150‑1 (MQ=255)
                                                                                                   gAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCa                                                  <  2:632998/150‑1 (MQ=255)
                                                                                                   gAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCa                                                  >  1:1448077/1‑150 (MQ=255)
                                                                                                      gTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAgg                                               >  2:1140487/1‑150 (MQ=255)
                                                                                                      gTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAgg                                               <  1:397230/150‑1 (MQ=255)
                                                                                                      gTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGGGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAgg                                               >  2:1978822/1‑150 (MQ=255)
                                                                                                           ggggTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTCGCGCCGGGGATCAAGGTcgcg                                          >  2:986243/1‑150 (MQ=255)
                                                                                                           ggggTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGTGATCAAGGTcgcg                                          <  1:1408391/150‑1 (MQ=255)
                                                                                                             ggTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCa                                        >  1:1000188/1‑150 (MQ=255)
                                                                                                              gTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAg                                       <  2:1067565/150‑1 (MQ=255)
                                                                                                                ttGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgc                                     <  1:198663/150‑1 (MQ=255)
                                                                                                                  gATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgccg                                   >  1:276367/1‑150 (MQ=255)
                                                                                                                    tatGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgccgcc                                 >  1:1629033/1‑150 (MQ=255)
                                                                                                                        gatgatGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGtt                             <  1:515112/150‑1 (MQ=255)
                                                                                                                          tgatgaGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGtttt                           <  2:1661459/150‑1 (MQ=255)
                                                                                                                              gagaCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCg                       <  2:1994484/150‑1 (MQ=255)
                                                                                                                                taCTTCAGTCATTCCAGCAGTGCTTGCTGGTGAGATTCGGTAGTTTTTAAAATCATCTGCACTTCGTTTTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTtcctcc                     <  2:1800656/149‑1 (MQ=255)
                                                                                                                                gaCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGcc                     >  1:1770677/1‑150 (MQ=255)
                                                                                                                                 aCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCa                    >  2:1385937/1‑150 (MQ=255)
                                                                                                                                  cTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAg                   >  2:509048/1‑150 (MQ=255)
                                                                                                                                  cTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGAAGCGCCGGAGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAg                   >  1:795930/1‑150 (MQ=255)
                                                                                                                                      tatctcaccAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTACACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACt               >  1:272976/8‑150 (MQ=255)
                                                                                                                                       ggCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCACAGTAATAGAGAGAGGTAGCGACGGGGATCAAGGTCGCTCAGGCCGCCAGTTTTTCCGCCAGCACtt              >  1:1739242/1‑150 (MQ=255)
                                                                                                                                         ctcaccAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTg            >  1:1779514/5‑150 (MQ=255)
                                                                                                                                         cATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTg            <  1:655268/150‑1 (MQ=255)
                                                                                                                                          tcaccAGCAGGCACTGCTGGTGAGATTCGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTgg           >  1:1599797/4‑150 (MQ=255)
                                                                                                                                          tcaccAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTgg           >  1:1382193/4‑150 (MQ=255)
                                                                                                                                           ttCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggg          >  2:1559397/1‑149 (MQ=255)
                                                                                                                                           ttCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggc          <  1:68523/150‑1 (MQ=255)
                                                                                                                                             ccAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcgg        >  1:1571659/1‑150 (MQ=255)
                                                                                                                                             ccAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcgg        >  1:1663087/1‑150 (MQ=255)
                                                                                                                                              cagcagTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcggc       >  2:183255/1‑150 (MQ=255)
                                                                                                                                                gcagTGCCTGCTGGTGAGATACGGTAGTTGTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCGAGTATTACAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCAAGTATTTCCGCCAGCACTTTGGCGGCTa     >  2:340562/1‑150 (MQ=255)
                                                                                                                                                  agTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCTCCAGCACTTTGGCGGCTaaa   >  1:1155870/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAAT  >  NC_000913/4364984‑4365280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: