Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,365,134 C→A 5.9% A75S (GCA→TCA) ‡ cutA ← divalent‑cation tolerance protein, copper sensitivity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,365,1340CA 5.9% 437.4 / 10.1 147A75S (GCA→TCA) ‡cutAdivalent‑cation tolerance protein, copper sensitivity
Reads supporting (aligned to +/- strand):  ref base C (71/67);  new base A (5/4);  total (76/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

CGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAATCC  >  NC_000913/4364985‑4365282
                                                                                                                                                     |                                                                                                                                                    
cGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTTCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGc                                                                                                                                                      >  1:1979539/1‑150 (MQ=255)
cGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGc                                                                                                                                                      <  1:705231/150‑1 (MQ=255)
   cAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGAGATGGATGATGAGCCTTCAGGCATTCCAGCAGTGCctg                                                                                                                                                   >  2:1542590/1‑150 (MQ=255)
    aaaaaCGTAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCACCGTGTGTAACAGGGAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCctgc                                                                                                                                                  <  1:1001277/150‑1 (MQ=255)
       aaCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAACCATGCGTTGAGCCATGAGAGGTAATCTTTGTCTCCGTGTGTAACAGGTATATCGAGAAGTTGCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTgg                                                                                                                                               <  2:836538/150‑1 (MQ=255)
        aCGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTgga                                                                                                                                              <  1:1349273/150‑2 (MQ=255)
         cGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTggaa                                                                                                                                             <  2:949641/150‑3 (MQ=255)
          ggAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGAGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTga                                                                                                                                            >  1:1067565/1‑150 (MQ=255)
              gTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGGGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGGCACTGCTggaatgcct                                                                                                                                        <  1:460316/150‑8 (MQ=255)
               tgctgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGAAAACTGTGTCTCCGTCTGTAACAGGTAAAAACAGAAGTTCCGGGGATTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATAc                                                                                                                                       >  2:922185/1‑150 (MQ=255)
                 ctgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACgg                                                                                                                                     >  2:249286/1‑150 (MQ=255)
                 ctgcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGAAATCTGTGTCTCCGTGTGTAACAGCTAAAACCAGAAGATCCGCGGTATGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTCCTGCTGAGATACgg                                                                                                                                     >  1:1679734/1‑150 (MQ=255)
                   gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTa                                                                                                                                   <  2:1535975/150‑1 (MQ=255)
                   gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTa                                                                                                                                   <  1:1272382/150‑1 (MQ=255)
                   gcAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTa                                                                                                                                   >  2:802150/1‑150 (MQ=255)
                    cAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAg                                                                                                                                  <  1:16144/150‑1 (MQ=255)
                            cAGTATCAGAGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCTTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATTATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTaaa                                                                                                                          <  1:1500020/150‑1 (MQ=255)
                             aGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTaaaa                                                                                                                         <  1:630057/150‑1 (MQ=255)
                                  cAGCGTAAAGATGCGTTGATCCATGAGAGGTAATCTGTGTCTCCGGGTGAAACAGGTAAAACCAGAAGTTCCGTGGTTTGATATGGATGATTAGACTTCAGGCATACCACCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAatcatc                                                                                                                    <  1:187230/150‑1 (MQ=255)
                                    gCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTg                                                                                                                  >  1:1191561/1‑150 (MQ=255)
                                     cGTACAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGc                                                                                                                 <  2:1770436/150‑1 (MQ=255)
                                     cGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGc                                                                                                                 >  1:947384/1‑150 (MQ=255)
                                     cGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGc                                                                                                                 >  1:1356568/1‑150 (MQ=255)
                                           gATGCGTTTAGCCATGAGAGTTAATCTGTGTCTCCGTGTGTACAATGTAAAACCAGAAGATCCGTTGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCg                                                                                                           <  1:376242/150‑1 (MQ=255)
                                           gATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGCCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTct                                                                                                           >  1:49529/1‑149 (MQ=255)
                                           gATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGGTCAGGGGTTTGATATGGATGATGAGACTTCAGTCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTAAAAATCATCTGCACTTCg                                                                                                           <  2:1217306/150‑1 (MQ=255)
                                               cGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTAtt                                                                                                       <  1:937213/150‑1 (MQ=255)
                                                 ttGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCt                                                                                                     <  2:507/150‑1 (MQ=255)
                                                   gAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTg                                                                                                   >  1:1994484/1‑150 (MQ=255)
                                                       cATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTcc                                                                                               >  1:1890630/1‑150 (MQ=255)
                                                       cATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTcc                                                                                               >  1:1738778/1‑150 (MQ=255)
                                                             agGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTTAAATCATCTGCACGTCGTATTCTTGGTCCAGCTTa                                                                                         >  2:285915/1‑150 (MQ=255)
                                                              ggTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTAc                                                                                        >  1:656668/1‑150 (MQ=255)
                                                              ggTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTAc                                                                                        <  2:527665/150‑1 (MQ=255)
                                                                   tCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTcc                                                                                   >  2:714658/1‑150 (MQ=255)
                                                                   tCTGTGTCTCCGTGGGTAAACGGTATAACCAGAAGTTCCGGGGTTTGATATGGATGTTGAGACTTCAGGCATTCCAGCTGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTcc                                                                                   <  1:366061/150‑1 (MQ=255)
                                                                    cTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTccc                                                                                  <  2:26019/150‑1 (MQ=255)
                                                                      gtgtCTCCGTGTGTAACAGGTAACACCAGAAGTTCCGGGGGTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCGTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGATCCAGCTTACCTTccccg                                                                                >  2:379511/1‑148 (MQ=255)
                                                                      gtgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAg                                                                                <  2:565770/150‑1 (MQ=255)
                                                                      gtgtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTGTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAg                                                                                <  2:1958061/150‑1 (MQ=255)
                                                                        gtCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTa                                                                              >  2:1478744/1‑150 (MQ=255)
                                                                           tcCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATa                                                                           <  2:1908862/150‑1 (MQ=255)
                                                                                gtgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagag                                                                      >  1:99752/1‑150 (MQ=255)
                                                                                gtgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCAAGTAATagagag                                                                      >  2:608434/1‑150 (MQ=255)
                                                                                 tgtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagaga                                                                     <  2:650859/150‑1 (MQ=255)
                                                                                  gtAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATagagagag                                                                    >  2:1853940/1‑150 (MQ=255)
                                                                                   tAACAGGTAAAACCAGAAGTTCAGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCAGCTGGAGAGATACGGTTGTTTTTAAAAACATCTGCACTTCGTATTCTTGCTCCAGCTAACATTCCCAGTAATAGAGAGAgg                                                                   >  2:93519/1‑150 (MQ=255)
                                                                                    aaCAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGt                                                                  >  1:1953423/1‑150 (MQ=255)
                                                                                      cAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAg                                                                <  2:583195/150‑1 (MQ=255)
                                                                                      cAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAg                                                                >  1:226110/1‑150 (MQ=255)
                                                                                      cAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAg                                                                >  1:831663/1‑150 (MQ=255)
                                                                                      cAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAg                                                                <  2:1583018/150‑1 (MQ=255)
                                                                                        ggTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGATCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAgcg                                                              >  1:1111358/1‑150 (MQ=255)
                                                                                          tAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGcc                                                            <  1:233602/150‑1 (MQ=255)
                                                                                           aaaaCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTTATAGAGAGAGGTAGCGCCg                                                           >  2:121500/1‑150 (MQ=255)
                                                                                           aaaaCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCg                                                           >  2:1467062/1‑150 (MQ=255)
                                                                                             aaCCAGAACTTCCTGGGTTTGATATGGATGATGCGACTTCAGTCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAATTCATCTCCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGGAGCGCCggg                                                         <  1:1814867/150‑1 (MQ=255)
                                                                                              aCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                        >  2:417098/1‑150 (MQ=255)
                                                                                              aCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                        >  1:1169580/1‑150 (MQ=255)
                                                                                              aCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                        >  1:57353/1‑150 (MQ=255)
                                                                                              aCCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCgggg                                                        >  1:1823337/1‑150 (MQ=255)
                                                                                               ccAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGa                                                       >  2:1311465/1‑150 (MQ=255)
                                                                                                  gAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCa                                                    <  1:934848/150‑1 (MQ=255)
                                                                                                  gAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCa                                                    >  1:1448077/1‑150 (MQ=255)
                                                                                                     ggtCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAgg                                                 <  2:1770942/148‑1 (MQ=255)
                                                                                                     gTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAgg                                                 <  1:397230/150‑1 (MQ=255)
                                                                                                     gTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGGGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAgg                                                 >  2:1978822/1‑150 (MQ=255)
                                                                                                          ggggTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTCGCGCCGGGGATCAAGGTcgcg                                            >  2:986243/1‑150 (MQ=255)
                                                                                                          ggggTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGTGATCAAGGTcgcg                                            <  1:1408391/150‑1 (MQ=255)
                                                                                                           gggTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTATCGCCGGGGATCAAGGTCgcgc                                           <  2:1370022/150‑1 (MQ=255)
                                                                                                            ggTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCa                                          >  1:1000188/1‑150 (MQ=255)
                                                                                                             gTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAg                                         <  2:1067565/150‑1 (MQ=255)
                                                                                                               ttGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgc                                       <  1:198663/150‑1 (MQ=255)
                                                                                                                 gATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgccg                                     >  1:276367/1‑150 (MQ=255)
                                                                                                                   tatGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGgccgcc                                   >  1:1629033/1‑150 (MQ=255)
                                                                                                                       gatgatGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGtt                               <  1:515112/150‑1 (MQ=255)
                                                                                                                         tgatgaGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGtttt                             <  2:1661459/150‑1 (MQ=255)
                                                                                                                            tgaGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTcc                          <  2:2014817/150‑1 (MQ=255)
                                                                                                                            tgaGACTTCACGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTGCGTATTCTTGCTCCAGCATACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTcc                          <  2:1102550/150‑1 (MQ=255)
                                                                                                                             gagaCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCg                         <  2:1994484/150‑1 (MQ=255)
                                                                                                                               gaCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGcc                       >  1:1770677/1‑150 (MQ=255)
                                                                                                                                aCTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCa                      >  2:1385937/1‑150 (MQ=255)
                                                                                                                                 cTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGAAGCGCCGGAGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAg                     >  1:795930/1‑150 (MQ=255)
                                                                                                                                  ttCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGc                    <  2:361216/150‑1 (MQ=255)
                                                                                                                                     tatctcaccAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTACACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACt                 >  1:272976/8‑150 (MQ=255)
                                                                                                                                      ggCATTCCAGCAGTGCCTGCTGGTGTGATACGGTAGTTTTTAAAATCATCTGCTGTTCGTATTCTTGCTCCAGGTTACCTTCCCAGTACTAGAGAGATGTAGGGCCGGTGATCAAGGTCGCGCAGGGCGCCAGTTTTTCCGCCAGCACtt                <  2:1679734/150‑1 (MQ=255)
                                                                                                                                      ggCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCACAGTAATAGAGAGAGGTAGCGACGGGGATCAAGGTCGCTCAGGCCGCCAGTTTTTCCGCCAGCACtt                >  1:1739242/1‑150 (MQ=255)
                                                                                                                                        ctcaccAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTg              >  1:1779514/5‑150 (MQ=255)
                                                                                                                                        cATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTg              <  1:655268/150‑1 (MQ=255)
                                                                                                                                         tcaccAGCAGGCACTGCTGGTGAGATTCGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTgg             >  1:1599797/4‑150 (MQ=255)
                                                                                                                                         tcaccAGCAGGCACTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTgg             >  1:1382193/4‑150 (MQ=255)
                                                                                                                                          ttCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggg            >  2:1559397/1‑149 (MQ=255)
                                                                                                                                          ttCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggc            <  1:68523/150‑1 (MQ=255)
                                                                                                                                            ccAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcgg          >  1:1571659/1‑150 (MQ=255)
                                                                                                                                            ccAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcgg          >  1:1663087/1‑150 (MQ=255)
                                                                                                                                             cagcagTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTggcggc         >  2:183255/1‑150 (MQ=255)
                                                                                                                                               gcagTGCCTGCTGGTGAGATACGGTAGTTGTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCGAGTATTACAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCAAGTATTTCCGCCAGCACTTTGGCGGCTa       >  2:340562/1‑150 (MQ=255)
                                                                                                                                                 agTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCTCCAGCACTTTGGCGGCTaaa     >  1:1155870/1‑150 (MQ=255)
                                                                                                                                                    gCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAATcc  >  2:1407631/1‑150 (MQ=255)
                                                                                                                                                    gCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAATcc  <  2:1830141/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGGCAAAAACGGAAGTGCTGCAAAGTAGCAGGATCAGCGTAAAGATGCGTTGAGCCATGAGAGGTAATCTGTGTCTCCGTGTGTAACAGGTAAAACCAGAAGTTCCGGGGTTTGATATGGATGATGAGACTTCAGGCATTCCAGCAGTGCCTGCTGGTGAGATACGGTAGTTTTTAAAATCATCTGCACTTCGTATTCTTGCTCCAGCTTACCTTCCCAGTAATAGAGAGAGGTAGCGCCGGGGATCAAGGTCGCGCAGGCCGCCAGTTTTTCCGCCAGCACTTTGGCGGCTAAATCC  >  NC_000913/4364985‑4365282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: