Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,390,406 T→G 5.2% intergenic (‑46/+51) psd ← / ← rsgA phosphatidylserine decarboxylase/ribosome small subunit‑dependent GTPase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,390,4060TG 5.2% 431.2 / 9.4 145intergenic (‑46/+51)psd/rsgAphosphatidylserine decarboxylase/ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base T (72/65);  new base G (4/4);  total (76/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

Only 100 of 146 total aligned reads displayed.

TTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTCCC  >  NC_000913/4390258‑4390555
                                                                                                                                                    |                                                                                                                                                     
ttGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATg                                                                                                                                                      >  1:1643399/1‑150 (MQ=255)
   tCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCg                                                                                                                                                   >  1:1200382/1‑150 (MQ=255)
    cAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGCTCCGGCCTACATGCCgg                                                                                                                                                  >  2:1711878/1‑150 (MQ=255)
      gCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGAt                                                                                                                                                <  2:65838/150‑1 (MQ=255)
       ccATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATc                                                                                                                                               >  1:1383761/1‑150 (MQ=255)
       ccATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATc                                                                                                                                               <  1:1565533/150‑1 (MQ=255)
        cATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATcc                                                                                                                                              <  2:1125771/150‑1 (MQ=255)
        cATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATcc                                                                                                                                              <  1:721749/150‑1 (MQ=255)
         aTCCGGCCCGCTTGCTTGCGCCCCACCCCGCCCGGCGAGTAAGCCCTAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCATGTAGGCCGGATcag                                                                                                                                             <  1:1800999/150‑3 (MQ=255)
          tCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTg                                                                                                                                            <  2:1634903/150‑1 (MQ=255)
           ccTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGa                                                                                                                                           >  1:590717/1‑150 (MQ=255)
           ccTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGa                                                                                                                                           <  1:351672/150‑1 (MQ=255)
            cTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaa                                                                                                                                          >  2:1539739/1‑150 (MQ=255)
            cTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACAGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGACTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaa                                                                                                                                          >  2:1801484/1‑150 (MQ=255)
             tGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCGGaaa                                                                                                                                         >  1:318982/1‑150 (MQ=255)
              gCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCTCAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCCGCATGTAGGCCGGATcaggaacg                                                                                                                                        <  2:1007263/150‑8 (MQ=255)
               cccGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaaaaa                                                                                                                                       >  1:1087951/1‑150 (MQ=255)
                ccGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGATTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTCTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaaaaaa                                                                                                                                      >  1:1298211/1‑150 (MQ=255)
                ccGCTTGCTTGCGCCCCAACCCGACAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTATAGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTACTGATCCGGCCTAAATGCCGGATCCTGaaaaaa                                                                                                                                      >  1:933174/1‑150 (MQ=255)
                  gcttgcttGCGCCCCAACCCGCCAGGCGAGTAAGTCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAg                                                                                                                                    <  2:1417262/150‑1 (MQ=255)
                   cttgcttgCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACCTGCCGGATCCTGAAAAAAAgg                                                                                                                                   >  2:648860/1‑150 (MQ=255)
                   cttgcttgCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAgg                                                                                                                                   <  2:585167/150‑1 (MQ=255)
                    ttgcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAggg                                                                                                                                  <  1:677256/150‑1 (MQ=255)
                    ttgcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAggg                                                                                                                                  <  1:827867/150‑1 (MQ=255)
                    ttgcgtgcGCCCCAACCCGCCAGGCGAGTAACCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAggg                                                                                                                                  <  2:1089370/150‑1 (MQ=255)
                      gcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTATAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTTTCGTTCCTGATCAGGCCTACATGCCGGATCCTGAAAAAAAggggc                                                                                                                                >  2:1226336/1‑149 (MQ=255)
                       cttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAATAAGGGGAc                                                                                                                               >  1:392360/1‑150 (MQ=255)
                       cttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGAc                                                                                                                               <  1:11181/150‑1 (MQ=255)
                        ttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACg                                                                                                                              >  1:1727408/1‑150 (MQ=255)
                        ttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGCCCTACATGCCGGATCCTGAAAAAAAGGGGACg                                                                                                                              >  2:168886/1‑150 (MQ=255)
                         tgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGa                                                                                                                             >  1:1489236/1‑150 (MQ=255)
                         tgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGGTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGa                                                                                                                             <  1:327334/150‑1 (MQ=255)
                           cgcCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGAtt                                                                                                                           >  1:1594896/1‑150 (MQ=255)
                            gcCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTc                                                                                                                          <  1:1554520/150‑1 (MQ=255)
                              cccAACCGGCCAGGCGAGTAAGCCTTAGTTTCGGCTGAATGTAGTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTTTCGTTCCTGATCCGTCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTa                                                                                                                        <  1:1809840/150‑1 (MQ=255)
                              cccAACCAGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCAGTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGAGCCGGAAAAAAAGGGGACGATTCTa                                                                                                                        >  1:1918513/1‑150 (MQ=255)
                                cAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAac                                                                                                                      <  2:1488533/150‑1 (MQ=255)
                                   cccGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAacgac                                                                                                                   >  1:1143937/1‑150 (MQ=255)
                                      gCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGt                                                                                                                <  2:527004/150‑1 (MQ=255)
                                        cAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTa                                                                                                              >  1:1432329/1‑150 (MQ=255)
                                                 aaGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTg                                                                                                     >  1:1543182/1‑150 (MQ=255)
                                                  aGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgt                                                                                                    >  2:1335151/1‑150 (MQ=255)
                                                    ccATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtca                                                                                                  >  1:20953/1‑150 (MQ=255)
                                                     cATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcag                                                                                                 <  1:1233605/150‑1 (MQ=255)
                                                     cATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcag                                                                                                 <  1:1393817/150‑1 (MQ=255)
                                                        aGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcagtca                                                                                              >  1:1218369/1‑150 (MQ=255)
                                                         gTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcagtgcc                                                                                             <  1:1373057/150‑4 (MQ=255)
                                                           ttCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATcc                                                                                           <  2:507283/150‑1 (MQ=255)
                                                            tCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCg                                                                                          <  2:1568360/150‑1 (MQ=255)
                                                            tCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCg                                                                                          >  2:465700/1‑150 (MQ=255)
                                                            tCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCg                                                                                          <  1:2006215/150‑1 (MQ=255)
                                                                 aGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCa                                                                                     <  2:800715/150‑1 (MQ=255)
                                                                 aGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCa                                                                                     <  1:1630636/150‑1 (MQ=255)
                                                                  gAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCTTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAg                                                                                    >  2:896235/1‑150 (MQ=255)
                                                                    aTGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGaa                                                                                  >  1:351119/1‑150 (MQ=255)
                                                                       tACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTTATCCGGCCTAAATGCCGTATCCTGAAAAAAAGGGGACGATTCTAACTACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAg                                                                               <  1:945074/150‑1 (MQ=255)
                                                                       tACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAg                                                                               <  1:1757302/150‑1 (MQ=255)
                                                                         cTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGtt                                                                             >  2:1771017/1‑150 (MQ=255)
                                                                         cTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGtt                                                                             >  1:2013115/1‑150 (MQ=255)
                                                                              gCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTaa                                                                        >  1:1492373/1‑149 (MQ=255)
                                                                              gCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTAc                                                                        >  1:828855/1‑150 (MQ=255)
                                                                               cGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTAcg                                                                       >  2:1136308/1‑150 (MQ=255)
                                                                                gAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTAcgc                                                                      >  1:1737170/1‑150 (MQ=255)
                                                                                   aGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGtt                                                                   <  2:841072/150‑1 (MQ=255)
                                                                                    gTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGttt                                                                  >  2:1862126/1‑150 (MQ=255)
                                                                                     tttAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGtttt                                                                 >  1:1304283/1‑150 (MQ=255)
                                                                                     tttAAATGAAATTTACAAGGTAGCCTGGAGTCCTTTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTTAAAATAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGtttt                                                                 <  1:412788/150‑1 (MQ=255)
                                                                                      ttAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGttttt                                                                <  1:815073/150‑1 (MQ=255)
                                                                                       tAAATGAATTTAAGAATGTAGCCTACATGCCATTGTTTTGTCGTTCCTGATCCGGACTACATGCCGGATCCTTAAATAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTa                                                               <  2:1281361/150‑1 (MQ=255)
                                                                                       tAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTa                                                               <  1:997914/150‑1 (MQ=255)
                                                                                         aaTGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTAcc                                                             >  1:480060/1‑150 (MQ=255)
                                                                                         aaTGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTATTACGCGTTTTTAcc                                                             >  2:425264/1‑150 (MQ=255)
                                                                                              aTTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTgcgc                                                        <  2:208431/150‑1 (MQ=255)
                                                                                              aTTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGGTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTgcgc                                                        >  2:733814/1‑150 (MQ=255)
                                                                                                 tAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCAt                                                     >  1:977250/1‑150 (MQ=255)
                                                                                                  aaCAATGTAGCCTCAAGGCAATTGTTTTGACGTTCATGATCCGGCCTACATGCCGGATCCTGAAAAAAACGGCACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATg                                                    <  1:285291/150‑1 (MQ=255)
                                                                                                      aGGTAGCCTCCAGGACATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAATTTTTACGCGTTTTTACCTGCGCCATGCttt                                                >  2:1487407/1‑150 (MQ=255)
                                                                                                        gTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTcc                                              <  2:1989753/150‑1 (MQ=255)
                                                                                                          aGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAg                                            <  1:1259231/150‑1 (MQ=255)
                                                                                                          aGCCTCCAGGCCATTGTTTTGTCGGTCCTGATCCGGCCTACATTCCGGATCCTGAAAAAAAGGGGCCGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAg                                            <  2:323937/150‑1 (MQ=255)
                                                                                                          aGCCTCCAGGCCATTGTTTTGGCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAg                                            <  1:1259342/150‑1 (MQ=255)
                                                                                                                 aGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACgg                                     <  2:697855/150‑1 (MQ=255)
                                                                                                                  ggCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGTGACGAATCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGATTTTACCTGCGCCATGCTTTCCAGAATACGGt                                    <  2:1808952/150‑1 (MQ=255)
                                                                                                                   gCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCATGATCCTGAAAAAATGGGGACGATTCTAACGACTGTTAGCTTAATTGTCAGTCATCAGTATCAGAAAAGTTTTTACTCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTg                                   <  2:933174/150‑1 (MQ=255)
                                                                                                                    ccATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGa                                  >  1:1024775/1‑150 (MQ=255)
                                                                                                                     cATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGAt                                 <  2:638165/150‑1 (MQ=255)
                                                                                                                             tGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCg                         <  1:465700/150‑1 (MQ=255)
                                                                                                                                cGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCCTGCTTTCCAGAAGACGGTGATTGTTTTCGaaa                      >  1:317702/1‑150 (MQ=255)
                                                                                                                                   tCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACgg                   >  1:1936536/1‑150 (MQ=255)
                                                                                                                                    ccTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACggg                  <  2:1919367/150‑1 (MQ=255)
                                                                                                                                     cTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGt                 >  1:1471940/1‑150 (MQ=255)
                                                                                                                                     aggATCCGGCATGTAGGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGt                 >  2:352749/3‑150 (MQ=255)
                                                                                                                                      tGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAAGACGGTGATAGTTTTCGAAACGGGtt                >  1:91766/1‑150 (MQ=255)
                                                                                                                                       gATCCGGCATGTAGGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGtta               >  2:1415134/1‑149 (MQ=255)
                                                                                                                                            ggCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCcgcg          <  2:1087951/150‑1 (MQ=255)
                                                                                                                                              ccTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGAt        <  2:417256/150‑1 (MQ=255)
                                                                                                                                                tACATGCCGGATCCTGAAAAAAAGGGGACGCTTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGCttt      >  2:295324/1‑150 (MQ=255)
                                                                                                                                                   aTGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTcc   <  2:318982/150‑1 (MQ=255)
                                                                                                                                                   aTGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTcc   <  2:1613837/150‑1 (MQ=255)
                                                                                                                                                    tGCCGGATCCTGAAAAAAAGGGGACGATTCAAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTccc  <  1:919962/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTCCC  >  NC_000913/4390258‑4390555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: