Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,392,506	C→G	16.0%	noncoding (35/76 nt)	glyX →	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,506	0	C	G	16.0%	59.8 / 10.4	37	noncoding (35/76 nt)	glyX	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (13/18);  new base G (4/2);  total (17/20)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCG  >  NC_000913/4392357‑4392653                                                                                                                                                      |                                                                                                                                                    taCGCCGGAATAGATCAGTTGTTAGTGCTCGTGCTTGCGTAGTGCGGGGTCGTGATTTGGAGTTTCGTTTCCCTCGCCTAAATTTGAAAATTCCTGCAAACCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGc                                                                                                                                                     <  2:1020882/150‑1 (MQ=9)           tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtg                                                                                                                                           <  1:1966414/150‑3 (MQ=255)            atcTCAGTTTGTAGAGCACTACCTTGCCAAGTTCGGGGTCGCGATTTCGAGTCTCGTTTCCCTCTCCAAAATTTGAAAAGTGCTTCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgggg                                                                                                                                          <  1:1838683/148‑1 (MQ=14)            aGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgc                                                                                                                                          <  1:1523977/150‑4 (MQ=255)                 cggtgttAGCGCACGACCTTGGCCAGGTCGGGGGCGCGAGTTCGAGTCTCGGTTCCCGCTCCAAAATTTGAACAGTGCGGCACAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg                                                                                                                                     <  1:531514/144‑1 (MQ=11)                   ttGGTAGAGCACGACCTTGCCAAGGGCGGGGTCGCGAGTGCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAg                                                                                                                                   <  1:1824745/150‑1 (MQ=255)                    ttgTAGAGCACGACATGGCGAATGTCGGGGTCGCTAGTTCGACTCGCGTTTCCGGCGCATAAATTTGAAAAGTCCTTCAACGCACATACCACGCAAGCGGGAATAGATCAGTTGGTAGAGCACGACCTTGCCTAGGTCGGGGTCGCGAGt                                                                                                                                  <  2:716570/148‑1 (MQ=11)                     gctAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                                                                                 <  1:623931/148‑1 (MQ=39)                      gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAAGACCTGAAAAGCACAGACCACCCCAGCGGGAATAGCTCAGTTGGTAGCGCACGACCAAGCCCAGGTCGGCGGCGCGAGGTc                                                                                                                                >  1:732608/1‑150 (MQ=11)                       tAGAGCACGACCTTGCCAAGGGCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCg                                                                                                                               <  1:1722663/150‑1 (MQ=255)                          ggctcGAGCTTGCGAATTTCGTGGTCGAGTGTTCGCGTCTCGTTTCCCGCTCATAAATTTGAAAAGTGCTTCAACTCAAATAACCCCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGt                                                                                                                            <  2:1878922/146‑1 (MQ=11)                           ggtcGACCTTGCGTAGGTCGGGGTCGCTATTTCGTGTCTCGTTTACCGCTCCAAAATTTGAAAAGTGCTGCACAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtc                                                                                                                           <  1:799571/147‑1 (MQ=14)                           gCACGACCTTCCCAAGGGCGGGGTCGCTAGATCGAGCCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCGCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtc                                                                                                                           <  1:1095035/150‑1 (MQ=14)                                    tGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                                                                  <  1:1811073/150‑1 (MQ=39)                                    tGCCAAGGGCGGGGTCGATATTTCGAGTCTCGTTACCCGCTCCAAAATTTGAAAAGTGCTTCAAAGCACATAACAACCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc                                                                                                                  <  1:821367/150‑1 (MQ=14)                                       cAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGACATTAAAAGCCCCAACCACCCAAGCGGGAATAGCTCAGTTTGTTTGTCACGAACTAGCCAATGTCGTCGTCACAAGTTCGAGTAGCGTTACCCggt                                                                                                               >  2:1453465/1‑148 (MQ=2)                                                    gctatttCGAGTCTCGTTTCCCGCTCAAAAATTTGAAAAGTTCTTCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaaa                                                                                                  <  1:579934/145‑1 (MQ=14)                                                        gTTCGAGGCTCGGTTCCCGCTCCAAAATTTGAAAAGTGCTCCATAGCACAGACCACCCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGc                                                                                              <  1:1693727/150‑1 (MQ=17)                                                           ggtgcctcGTTTCCCTCTCCTAAATTTGAAAAGTCCGCCAAATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt                                                                                           <  2:1098558/145‑1 (MQ=17)                                                               tctcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTAACAGCTCCAAAATTTGAAAGACCTGTaatg                                                                                       >  1:1479475/1‑148 (MQ=17)                                                                ctcGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGc                                                                                      >  1:1494601/1‑150 (MQ=255)                                                                 tcGGTTCCCGCTCAAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGca                                                                                     <  1:1462171/150‑1 (MQ=255)                                                                        ccGGTCCAAAATTTGAAAATTCCTGCACATCACATACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGacca                                                                              <  1:730737/150‑1 (MQ=18)                                                                        ccGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAACGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGGTCGAGTCTCGTTTCCCGCGCCAAATTGTGCAAGTGGTGTAAGGgaccgaccc                                                                              >  1:1927265/1‑149 (MQ=14)                                                                              cAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAg                                                                        >  2:673728/1‑150 (MQ=255)                                                                                 aaTTTGAAAAGTGCGTCACATCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCgg                                                                     <  1:517393/150‑1 (MQ=255)                                                                                         aaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATGTGAAAGAGATGAAATGAACACACCACCCCAGAGGGAATAGca                                                             >  1:1941645/1‑149 (MQ=17)                                                                                                gcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCACGCGGGAATAGCTAAGTTgg                                                      >  1:1536759/1‑150 (MQ=255)                                                                                                        aataccacgCAAGCGGGAATAGCTCCGTTGGTAGAGCACGACATTGCCAAGGTGGGGGTCGCTAGTTCGAGTCTCGTTACCCGCTCCAAAATTTTAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCAc                                              <  2:1117646/147‑1 (MQ=14)                                                                                                                 cAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTACCGCTCCAAAATTTGAAAGTGATGTAATGAACATACCACCCAAGCGGGATTATCTAAGTTGTTAGAGAAAGAccaatac                                     >  2:858790/1‑145 (MQ=11)                                                                                                                                    tGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTTAAAGTGATTTAATGAACACACCACCCCAGCGGGAAAATCAGAGTTTGTATAGCACGAACATGCCAAGTTGAGGGTAGAGAGtt                  >  2:1655896/1‑150 (MQ=2)                                                                                                                                        agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGATGTAAGGACCAGACCCCCCAAGAGGGAATAGCTCGGTTTGTAGAGAACGACAATTCCAAGTGCGGGGTCGCGTATTCgcg              >  1:1915260/1‑148 (MQ=11)                                                                                                                                          accccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCCCAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCAAGACCTTGCCAAGGTAGGGGTCGCGAGGTCGAGtg            >  1:1448074/5‑149 (MQ=11)                                                                                                                                          accccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTACCGCTCCAAAATTTTAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATATCTAAGTTGTTAGAGCAAGACCTTGCCAAGGTCGGGGTCGCTAGTTCGAGtc            >  2:432747/5‑150 (MQ=11)                                                                                                                                           ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGCAAGTGATTTAATGAACAGACCACCCCAGCGGGAATAGCGCAGTTGGTAGAGCCAGACCCTGCCAAGGTCAGGGTCGCGAGTTCGACtcg           >  1:1208080/4‑149 (MQ=11)                                                                                                                                           ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATATGAAAGATATTTAAGGCACACACCACCCCAACGGGAATATCTAAGTTGGTTTAGACCGACAATGACAAGGTAGGGGTCGCGTGTTCGAGtca           >  2:1592037/4‑149 (MQ=2)                                                                                                                                                   ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAATTTAAAGTTCTGAAAGGAACACACCACCCAAGAGCGAATAGCTGAGTTGTTATAGAACGACCATGCCAAGGTAGGGGACGCGAGTACCAGTCGCGTTTcgc   >  2:934243/1‑148 (MQ=11)                                                                                                                                                    tGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAACCGGGAATAGCTCAGTTGGTAGAGCACGACAGTGCCAAGGTCGGGGTCGCTAGTTCGAGTCTCGTTTCCCg  >  2:1473262/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                    TACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCG  >  NC_000913/4392357‑4392653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑