Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,434,081 T→A 5.3% intergenic (‑57/‑32) qorB ← / → ytfH NAD(P)H:quinone oxidoreductase/DUF24 family HxlR‑type putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,434,0810TA 5.3% 403.4 / 6.3 142intergenic (‑57/‑32)qorB/ytfHNAD(P)H:quinone oxidoreductase/DUF24 family HxlR‑type putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (65/68);  new base A (3/5);  total (68/74)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

ATAGCCACTATTTGGCTGGCAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTG  >  NC_000913/4433933‑4434229
                                                                                                                                                    |                                                                                                                                                    
aTAGCCACTATTTGGCTGGCAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACtttt                                                                                                                                                     <  2:1633332/150‑1 (MQ=255)
aTAGCCACTATTTGGCTGGCAGGAACCGTTTTCATCAAGGATGCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACtttt                                                                                                                                                     <  2:507557/150‑1 (MQ=255)
  agacACTATTTGGCTGGCAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGTTCATGGTAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTag                                                                                                                                                   <  1:1408439/147‑1 (MQ=255)
   gCCACTATTTGGCTGGCAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTagt                                                                                                                                                  >  2:267030/1‑150 (MQ=255)
   gCCACTATTTGGCTGGCAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTagt                                                                                                                                                  >  2:1646342/1‑150 (MQ=255)
    ccACTATTTGGCTGGCAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTagta                                                                                                                                                 <  2:753912/150‑1 (MQ=255)
     cACTATTTGGCTGGCAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTagtaa                                                                                                                                                >  1:558325/1‑150 (MQ=255)
        tATTTGGCTGGCAGGAACCGTTTTCAGCAAGGATTCAATAACATAGTGACCAAGTTGCCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTATTGTCTTGTGTTTAAATACGCTAACACCTAAACATACTTTTagtaagta                                                                                                                                             >  2:1310558/1‑150 (MQ=255)
          tttGGCTGGCAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAgtacg                                                                                                                                           <  2:1017929/150‑1 (MQ=255)
            tggctggcAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTAAaagtaagtttagg                                                                                                                                         <  2:1280843/150‑6 (MQ=255)
            tggctggcAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTAAaagtaagtttagg                                                                                                                                         <  2:1703388/150‑6 (MQ=255)
             ggctggcAGTAACCGTTTTCATCAAGGATTCAATAACATACTGACCAAGTTGGCCAGTGGCACCAGTAATAGAGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACTCTAACACCTAAACTTACTAAaagtaagtttaggt                                                                                                                                        <  1:114888/150‑7 (MQ=255)
             ggctggcAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAgtacgtac                                                                                                                                        <  2:635747/150‑1 (MQ=255)
                 ggcAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACaaaa                                                                                                                                    <  2:750575/150‑1 (MQ=255)
                   cAGGAACCGTTTTAATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAg                                                                                                                                  <  1:903088/150‑1 (MQ=255)
                    aGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAgg                                                                                                                                 <  1:2008036/150‑1 (MQ=255)
                    aGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAgg                                                                                                                                 <  1:744867/150‑1 (MQ=255)
                    aGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAACGTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAgg                                                                                                                                 <  1:202101/150‑1 (MQ=255)
                     ggAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGt                                                                                                                                >  2:617756/1‑150 (MQ=255)
                         ccGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGt                                                                                                                            <  2:692896/150‑1 (MQ=255)
                         ccGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGCCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTAACTTTTAGTAAGTACGTACAAAAAGGTAAGt                                                                                                                            <  2:168299/150‑1 (MQ=255)
                           gTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTAt                                                                                                                          >  2:924908/1‑150 (MQ=255)
                                catcaAGGATTCAATATCATAGTGACTAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTACATCTTTTTGTCTTGTTTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAAt                                                                                                                     <  1:294991/150‑1 (MQ=255)
                                catcaAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTATGTACGTACAAAAAGGTAAGTATGatat                                                                                                                     >  1:296496/1‑150 (MQ=255)
                                 atcaAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATg                                                                                                                    >  1:1831728/1‑150 (MQ=255)
                                 atcaAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATg                                                                                                                    <  2:58122/150‑1 (MQ=255)
                                 aacaAGGATTCAATAACATAGTGACAAAGTTGGCCAGAGGCACCACTAATAGCGATCATGGGAAGTCTCCATCGTTTTGACTTCTGTTAAAATACGCTAACACCAAAACTTACTATAAGTAAGTACCAACAAAAAGCTAAGTTTGAAATg                                                                                                                    >  1:1352057/3‑150 (MQ=255)
                                  tcaAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGa                                                                                                                   >  2:1136605/1‑150 (MQ=255)
                                   caAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAg                                                                                                                  <  1:659270/150‑1 (MQ=255)
                                   caAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAg                                                                                                                  >  2:620656/1‑150 (MQ=255)
                                   caAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAAAACGCTATCACCTAAACTTACTTTTAGTAAGTACGGACAAAAAGGTAAGTATGAAAGGAg                                                                                                                  >  2:288979/1‑150 (MQ=255)
                                      ggATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCa                                                                                                               >  2:330670/1‑150 (MQ=255)
                                       gATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAg                                                                                                              >  1:785002/1‑150 (MQ=255)
                                           cAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTTGTAAGTACGTACTAAAAGGTAAGTATGAAATGAGTCAGGttt                                                                                                          >  2:387545/1‑149 (MQ=255)
                                           aaaTAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTa                                                                                                          <  1:785791/149‑1 (MQ=255)
                                             aTAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTAAGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGc                                                                                                        >  1:256437/1‑150 (MQ=255)
                                              tAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCTATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGcc                                                                                                       <  1:1253763/150‑1 (MQ=255)
                                              tAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGcc                                                                                                       <  1:1596512/150‑1 (MQ=255)
                                               aaCATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCCCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCt                                                                                                      >  2:1879861/1‑150 (MQ=255)
                                                   tAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCg                                                                                                  <  2:902034/150‑1 (MQ=255)
                                                    aGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGc                                                                                                 <  1:1726078/150‑1 (MQ=255)
                                                       gACTAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACATAAACTTACTTTTAGTAAGTACGTACAAAAAGGGAAGTATGAAATGAGTCAGGTTAGCCTGTCGcaac                                                                                              <  2:105534/150‑1 (MQ=255)
                                                       gACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGcaac                                                                                              >  2:1547623/1‑150 (MQ=255)
                                                        aCCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCAACGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGACTGTCGcaaca                                                                                             >  1:1094392/1‑150 (MQ=255)
                                                           aaGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACt                                                                                          >  1:1852651/1‑150 (MQ=255)
                                                            aGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTg                                                                                         <  1:1105832/150‑1 (MQ=255)
                                                               tGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTATCACCTAAACTGACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGaaa                                                                                      >  1:1219464/1‑150 (MQ=255)
                                                                 gCCAGTGACACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAaga                                                                                    >  2:393619/1‑150 (MQ=255)
                                                                    aGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAggg                                                                                 <  1:1359129/150‑1 (MQ=255)
                                                                         cACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAAtc                                                                            >  2:363622/1‑150 (MQ=255)
                                                                         cACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAAtc                                                                            <  1:1155985/150‑1 (MQ=255)
                                                                           ccAGTAATAGCGATCATGGGAACTCTCCATCCTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTAAAAAAAGGTAAGTATGAAATGAGTCAGGTTACCCTGTCTCACCAACTGAAAGAGGGTAAactc                                                                          >  2:371382/1‑150 (MQ=255)
                                                                            cAGTAATAGCGATCATGGGAAGTCTCCATCGTTTAGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGATAATctct                                                                         <  2:1218680/150‑1 (MQ=255)
                                                                               tAATCGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGATTAAATACGCAAACACCTAAACTTACTTTTAGTAAGTACGTACAAACAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACATCTTAAAGAGGGTAATCTCTTTg                                                                      <  1:1232585/150‑1 (MQ=255)
                                                                                aaTAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGc                                                                     >  1:919502/1‑150 (MQ=255)
                                                                                 aTAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAATTATGAAATGAGTCAGGTTAGCCTGTCGCAACATCTGAAAGAGGGTAATCTCTTTgag                                                                    <  1:374307/150‑3 (MQ=255)
                                                                                 aTAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCg                                                                    <  1:584086/150‑1 (MQ=255)
                                                                                  tatcGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCgg                                                                   <  2:972173/147‑1 (MQ=255)
                                                                                    gCGATAATGGGAAGTCTCTATCGTTTTGTCTTGTGTTTAAATACTCTAACACCTATACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTTAAAGAGATTACCCTCtttcagttg                                                                 <  1:1431020/150‑7 (MQ=255)
                                                                                     cGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAAc                                                                >  2:1419504/1‑150 (MQ=255)
                                                                                     cGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAAc                                                                <  2:1241496/150‑1 (MQ=255)
                                                                                           tGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGcc                                                          <  1:834067/150‑1 (MQ=255)
                                                                                                  tctcCATCGTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTcgcgc                                                  <  1:518405/150‑1 (MQ=255)
                                                                                                    tcCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTcgcgcg                                                 >  2:803728/1‑150 (MQ=255)
                                                                                                      cATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAg                                               <  2:558325/150‑1 (MQ=255)
                                                                                                        tCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGgt                                             <  1:1313123/150‑1 (MQ=255)
                                                                                                          gTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTACTCTCTTTGCGGAACAGTGACCGTCGCGCGAGgtgt                                           >  2:1201896/1‑150 (MQ=255)
                                                                                                          gTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGAAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGgtgt                                           >  1:1670396/1‑150 (MQ=255)
                                                                                                                tCTTGTGTTTAAATTCGCTAACACCTAAACTTTCTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACTACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAac                                     <  1:1310558/150‑1 (MQ=255)
                                                                                                                 cTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAaca                                    <  1:101362/150‑1 (MQ=255)
                                                                                                                 cTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAaca                                    >  2:1076648/1‑150 (MQ=255)
                                                                                                                    gtgtTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGt                                 >  2:1186663/1‑150 (MQ=255)
                                                                                                                     tgtTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTc                                <  1:267030/150‑1 (MQ=255)
                                                                                                                     tgtTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTc                                >  2:1184867/1‑150 (MQ=255)
                                                                                                                     tgtTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTc                                <  1:615095/150‑1 (MQ=255)
                                                                                                                           aaTACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGc                          <  1:827473/150‑1 (MQ=255)
                                                                                                                           aaTACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCACGTCGCGCGAGGTGTTGAAACACGTCACCAGc                          >  2:798994/1‑150 (MQ=255)
                                                                                                                             tACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCATGTTAGCCTTTCGAAACAACTTAAAGAGGTTAATCTCTTTGCGGAACATTGACCGTCGAGCCAGGTGTTGAAACAAGTCACAAgact                        >  2:477719/1‑147 (MQ=255)
                                                                                                                              aCGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGt                       >  1:1619832/1‑150 (MQ=255)
                                                                                                                               cGCTAACACCTAAACTTACTTTTAGAAAGTACGTACAAAAAGGTAAGTATGAAAGGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGtt                      <  2:976265/150‑1 (MQ=255)
                                                                                                                                 cTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTgg                    >  2:1980401/1‑150 (MQ=255)
                                                                                                                                 cTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAAAAGTGCCCGTCGCTCGAGGTGTTGAAACACGTCACCAGCCGTTgg                    >  1:161158/1‑150 (MQ=255)
                                                                                                                                     cacCTAAACTTACTTTTAGTAAGTACGTACAAAACGGTAAGTAGGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTgggggg                <  1:1539787/150‑1 (MQ=255)
                                                                                                                                     cacCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTgggggg                <  1:857789/150‑1 (MQ=255)
                                                                                                                                     cacCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGGCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGTCGTTgggggg                <  1:620656/150‑1 (MQ=255)
                                                                                                                                      gtacgtacttactaAAAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGgt               >  2:185952/7‑150 (MQ=255)
                                                                                                                                      gtacgtacttactaAAAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGgt               >  2:1341918/7‑150 (MQ=255)
                                                                                                                                      acCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGgt               >  2:339747/1‑150 (MQ=255)
                                                                                                                                      acCTAAACTTACTTTTAGCAAGTAAGTACAAAAAGGTAAGTAAGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCTTCGCGCGAGGTGTTGAAACACGTCACAAGCCGTTGGGGGgt               >  2:1046113/1‑150 (MQ=255)
                                                                                                                                       ccTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGgtg              >  2:64494/1‑150 (MQ=255)
                                                                                                                                        acgtacttactaAAAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGgtgt             >  1:490566/5‑150 (MQ=255)
                                                                                                                                           aaCTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGGCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGa          <  1:1248786/150‑1 (MQ=255)
                                                                                                                                            acttacttTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACACCTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGAt         <  2:1326574/150‑1 (MQ=255)
                                                                                                                                            acttacttTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGACTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGAt         >  1:1481993/1‑150 (MQ=255)
                                                                                                                                                acttATAGTAATTACGTACAAAAAGGTAAGTATGAAATGAGACAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGTGGGTGTTGATTCtg     <  1:387545/150‑1 (MQ=255)
                                                                                                                                                 cttTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCtgg    <  2:1231336/150‑1 (MQ=255)
                                                                                                                                                 cttTTAGTAAGTACGAACAAAAAGGTCAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCtgg    <  1:1562889/150‑1 (MQ=255)
                                                                                                                                                  ttttAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCtggt   <  2:673973/150‑1 (MQ=255)
                                                                                                                                                  ttttAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGGCGCGCGAGGGGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCtggt   <  2:1094392/150‑1 (MQ=255)
                                                                                                                                                   tttAGTAAGTACGTACAAAAAGGTAAGTATCAAATGAGTCAGGTTAGCCTGTCGCAACACCTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCtggtg  <  2:1918537/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATAGCCACTATTTGGCTGGCAGGAACCGTTTTCATCAAGGATTCAATAACATAGTGACCAAGTTGGCCAGTGGCACCAGTAATAGCGATCATGGGAAGTCTCCATCGTTTTGTCTTGTGTTTAAATACGCTAACACCTAAACTTACTTTTAGTAAGTACGTACAAAAAGGTAAGTATGAAATGAGTCAGGTTAGCCTGTCGCAACAACTGAAAGAGGGTAATCTCTTTGCGGAACAGTGCCCGTCGCGCGAGGTGTTGAAACACGTCACCAGCCGTTGGGGGGTGTTGATTCTGGTG  >  NC_000913/4433933‑4434229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: