Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,470,952 T→C 6.6% intergenic (‑39/+34) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,470,9520TC 6.6% 359.2 / 10.2 120intergenic (‑39/+34)ridA/pyrIenamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base T (50/62);  new base C (3/5);  total (53/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 125 total aligned reads displayed.

CGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGC  >  NC_000913/4470803‑4471101
                                                                                                                                                     |                                                                                                                                                     
gggATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATGAACGCCCTGTACGTAAGGAACGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCAt                                                                                                                                                       <  2:1067670/149‑1 (MQ=255)
cGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCAt                                                                                                                                                       >  2:1972033/1‑150 (MQ=255)
cGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCAt                                                                                                                                                       <  2:1118313/150‑1 (MQ=255)
  gATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATta                                                                                                                                                     <  1:993570/150‑1 (MQ=255)
    tttACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATtata                                                                                                                                                   >  2:230800/1‑150 (MQ=255)
    tttACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATtata                                                                                                                                                   <  2:730552/150‑1 (MQ=255)
     ttACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAg                                                                                                                                                  >  1:1372775/1‑150 (MQ=255)
      taccttgATCTGACCGGAGGTTATGATTATATTGCCCTTATCTACGATTTGTACTTAAGGACCGATTGCTGCCTGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAATTTCTGGCTCATTATAgg                                                                                                                                                 <  1:1055852/144‑1 (MQ=255)
      tACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAgg                                                                                                                                                 <  1:1962005/150‑1 (MQ=255)
         cGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAg                                                                                                                                              <  2:1525042/150‑1 (MQ=255)
           ggATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGcc                                                                                                                                            <  1:1607459/150‑1 (MQ=255)
               cTGCCCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGAc                                                                                                                                        <  1:1583230/150‑1 (MQ=255)
               cTGACCGGAGGTGATGATCAAATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGAc                                                                                                                                        <  1:1666476/150‑1 (MQ=255)
                tGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACt                                                                                                                                       <  1:25085/150‑1 (MQ=255)
                  aCCGGAGGTGATGAGCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACttt                                                                                                                                     <  1:1100243/150‑1 (MQ=255)
                    ctgAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATTGGGAGCCAGACTTtta                                                                                                                                   <  1:219545/148‑1 (MQ=255)
                    cGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTtta                                                                                                                                   <  2:1400199/150‑1 (MQ=255)
                       atgTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACtttttacc                                                                                                                                <  1:934851/148‑5 (MQ=39)
                       aGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTttatta                                                                                                                                <  1:648644/150‑1 (MQ=255)
                        ggTGATGATGATATTGCCCAGATCAACGCCCTGTACGTAACGACCGAAAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCTTATAGGGAGCCAGACTTTTATTAc                                                                                                                               <  1:791028/150‑1 (MQ=255)
                        ggTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGTTAGCTGGCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCCCTATAATGAGCCAGACtttttaccg                                                                                                                               <  2:1487835/150‑6 (MQ=38)
                             tgatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTTGCTCATTATAGGGAGCCAGACTTTTATTACCAAcc                                                                                                                          >  1:143430/1‑150 (MQ=255)
                             tgatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAAcc                                                                                                                          >  2:889135/1‑150 (MQ=255)
                              gatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCg                                                                                                                         >  1:887727/1‑150 (MQ=255)
                               atCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGc                                                                                                                        >  1:771307/1‑150 (MQ=255)
                                  atatTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGGCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTAATTATAGGGAGCCTGACTTTAATTACCAACCGCaat                                                                                                                     >  2:769650/1‑150 (MQ=255)
                                  atatTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaat                                                                                                                     <  2:650796/150‑1 (MQ=255)
                                   tatTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaatt                                                                                                                    <  1:932337/150‑1 (MQ=255)
                                   tatTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaatt                                                                                                                    >  1:318221/1‑150 (MQ=255)
                                         ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTgg                                                                                                              >  1:1331046/1‑150 (MQ=255)
                                         ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTgg                                                                                                              >  1:1324489/1‑150 (MQ=255)
                                          cAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGc                                                                                                             <  2:5851/150‑1 (MQ=255)
                                           aGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGcc                                                                                                            <  2:380319/150‑1 (MQ=255)
                                            gATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCa                                                                                                           >  2:453641/1‑150 (MQ=255)
                                               cAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTATCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGca                                                                                                        >  2:1643992/1‑150 (MQ=255)
                                                   gCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcaccac                                                                                                    <  1:1201164/150‑1 (MQ=255)
                                                      cTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACAtt                                                                                                 >  2:1730710/1‑150 (MQ=255)
                                                         tACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATg                                                                                              >  1:566027/1‑150 (MQ=255)
                                                         tACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATg                                                                                              <  1:607076/150‑1 (MQ=255)
                                                          aCGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATgg                                                                                             <  1:732414/150‑1 (MQ=255)
                                                             tAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaa                                                                                          >  1:380389/1‑150 (MQ=255)
                                                               aGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaaaa                                                                                        >  1:672136/1‑150 (MQ=255)
                                                                 gACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAAct                                                                                      >  1:484713/1‑150 (MQ=255)
                                                                   ccGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAActct                                                                                    <  1:731214/150‑1 (MQ=255)
                                                                   ccGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTAGTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAActct                                                                                    >  2:1479126/1‑150 (MQ=255)
                                                                    cGATAGCTGCCGGTGCATTTTCCGTAGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCtt                                                                                   <  1:306654/150‑1 (MQ=255)
                                                                     gATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTATTATTACCAACCGCAATTAATTGGCCAGCACCACATTATTGGAAAACTCttt                                                                                  >  2:1087396/1‑150 (MQ=255)
                                                                         gCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTca                                                                              >  2:1003734/1‑150 (MQ=255)
                                                                         gCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTca                                                                              <  2:437683/150‑1 (MQ=255)
                                                                          cTGCCGGTGGATTTTCCGTAGCGATAGTTTTGCTAATGAATTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCGAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                                                             <  2:1348440/150‑1 (MQ=255)
                                                                          cTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                                                             <  2:1890142/150‑1 (MQ=255)
                                                                          cTGACGGTTCATTTTCCTTCGAGATAGTTTTGCTAATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTAATTATAGGTAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                                                             <  1:870945/150‑1 (MQ=255)
                                                                            gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTTCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcacat                                                                           >  2:216330/1‑149 (MQ=255)
                                                                            gCCGGAGCATTTTCCGTCTCGTAAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAATTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAg                                                                           <  2:1909805/150‑1 (MQ=255)
                                                                                  gCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTg                                                                     <  2:318221/150‑1 (MQ=255)
                                                                                  gCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTg                                                                     <  2:1939501/150‑1 (MQ=255)
                                                                                  gCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTg                                                                     >  1:5790/1‑150 (MQ=255)
                                                                                  gCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTg                                                                     <  2:2014104/150‑1 (MQ=255)
                                                                                    aTTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCa                                                                   <  2:732067/150‑1 (MQ=255)
                                                                                     ttttCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAt                                                                  <  1:1916398/150‑1 (MQ=255)
                                                                                             cgcgATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTAACAGTATTTGCATTTGAgcgc                                                          >  1:1918216/1‑150 (MQ=255)
                                                                                              gcgATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGcgcg                                                         <  1:368946/150‑1 (MQ=255)
                                                                                              gcgATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGcgcg                                                         >  2:596872/1‑150 (MQ=255)
                                                                                                gATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGat                                                       <  2:160687/150‑1 (MQ=255)
                                                                                                      tttGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCAtt                                                 <  2:887727/150‑1 (MQ=255)
                                                                                                       ttGCACATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTg                                                <  1:727710/150‑1 (MQ=255)
                                                                                                        tGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAAACGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTgg                                               >  1:1809707/1‑150 (MQ=255)
                                                                                                          cTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACAGCAATTAATTGGCCAGCACCACATTATGTGAAAACTCTTTTTAACAGTATTTGCATTTGAGAGCGATATCAGTGgcg                                             >  1:130052/1‑150 (MQ=255)
                                                                                                            cATTATATCTCCTTTATTACAGCGTTAAAAAGTATTGCTAATTATAGTGATCAAGACTTTTATTACCATCCTCAATTAAATGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTTCATTTTAGCGCGATATCATTGGcgcg                                           <  2:734247/150‑1 (MQ=255)
                                                                                                             aTGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGt                                          <  1:1998598/150‑1 (MQ=255)
                                                                                                                aTTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGtttt                                       <  1:1987778/150‑1 (MQ=255)
                                                                                                                  ttCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCg                                     <  1:1191878/150‑1 (MQ=255)
                                                                                                                   tctcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGc                                    <  1:1972033/150‑1 (MQ=255)
                                                                                                                   tctcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGc                                    >  1:497919/1‑150 (MQ=255)
                                                                                                                    ctcCTTTATTACAGCGGTAAAAATTCTTGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa                                   <  1:965010/150‑1 (MQ=255)
                                                                                                                    ctcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa                                   <  2:452554/150‑1 (MQ=255)
                                                                                                                    ctcCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAACAACCGCAATTAATTGGCCACAACAACATTATGGGAAAACTCTTTTTCACACTATTTGCATTTGAGCGCGATATCATTGGCGCTTTTTCGCa                                   >  2:899440/1‑150 (MQ=255)
                                                                                                                       cTTTATTACAGCGGTAAAAAGTCTGGCTCTTTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGAACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg                                >  1:1206439/1‑150 (MQ=255)
                                                                                                                       cTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg                                >  2:902111/1‑150 (MQ=255)
                                                                                                                        tttATTACAGCGGTAAAACGTCGGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGAAATTAATTGGCCAGCACCACATTATGGGAAACCTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGc                               <  1:1443071/150‑1 (MQ=255)
                                                                                                                          tattaCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaa                             >  2:1697714/1‑150 (MQ=255)
                                                                                                                          tattaCAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaa                             >  1:1940700/1‑150 (MQ=255)
                                                                                                                                  cGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGAt                     <  1:225110/150‑1 (MQ=255)
                                                                                                                                     tAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaa                  >  2:754624/1‑150 (MQ=255)
                                                                                                                                      aaaaaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTATTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGaca                 >  2:82188/1‑148 (MQ=255)
                                                                                                                                      aaaaaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCAAAGTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGAGCGATATAATTGGCGCGTTTTCGCACCGAAAAGCAGGATGaaa                 >  2:1490836/1‑150 (MQ=255)
                                                                                                                                        aaaGTCTGGCTCCTTCTAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAACCTCTTTTTCCCAGAATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAACGCTGGATGAAAcc               <  1:275327/150‑1 (MQ=255)
                                                                                                                                        aaaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCCACCGCAATTAATTGGCCCGCACCAAATTATGGGAAAACTCTTGTTCACACTATTTGCATTTGAGCGCGATATCAATGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAcc               >  1:1161133/1‑150 (MQ=255)
                                                                                                                                        aaaGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAcc               >  1:1525022/1‑150 (MQ=255)
                                                                                                                                          aGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCgg             <  2:566027/150‑1 (MQ=255)
                                                                                                                                           gTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGt            >  1:10128/1‑150 (MQ=255)
                                                                                                                                            tCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGtt           >  2:814229/1‑150 (MQ=255)
                                                                                                                                             cTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTc          >  1:1568362/1‑150 (MQ=255)
                                                                                                                                             cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTc          >  2:1315108/1‑150 (MQ=255)
                                                                                                                                             cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTc          >  1:829891/1‑150 (MQ=255)
                                                                                                                                                gCTCATTATAGGGAGCCAGACTTTTATTACCAAGCGCAATTAATTGGCCAGCACCACATTATGTGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGTCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGc       <  1:1087396/150‑1 (MQ=255)
                                                                                                                                                gCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGc       >  1:1427859/1‑150 (MQ=255)
                                                                                                                                                   cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATg    <  2:1074506/150‑1 (MQ=255)
                                                                                                                                                     ttATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGc  <  2:497919/150‑1 (MQ=255)
                                                                                                                                                     ttATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGc  <  1:620494/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAAAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGGC  >  NC_000913/4470803‑4471101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: