BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,502,107	G→T	5.3%	A442D (GCT→GAT)	insG ←	IS4 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,502,107	0	G	T	5.3%	394.4 / 5.5	134	A442D (GCT→GAT)	insG	IS4 transposase
Reads supporting (aligned to +/- strand): ref base G (65/60); new base T (4/4); total (69/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

CATGACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGT  >  NC_000913/4501959‑4502256

cATGACAATGTCAAACAGTCAAAGATTGTGGCGTTGCCAGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGc                                                                                                                                                      <  2:820874/150‑1 (MQ=255)

  tGACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCaa                                                                                                                                                    >  1:2196/1‑150 (MQ=255)

  tGACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCaa                                                                                                                                                    >  2:211849/1‑150 (MQ=255)

   gACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAac                                                                                                                                                   >  1:928604/1‑150 (MQ=255)

   gACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCGCAGTATTCTCGTTGGCAACCAGATTATTTAACAGTTGTTGCTGAAACGATATAGGCATCTTGGTTCGGTACTGCCAGTCAGTTACGCAac                                                                                                                                                   >  2:347803/1‑150 (MQ=255)

   gACAATGTCAAACAGGCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAac                                                                                                                                                   <  2:991560/150‑1 (MQ=255)

    aCAATGTCAAACAGTGAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCATCCAGATAATTTAACAGTTGATGCTGATACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAact                                                                                                                                                  <  1:40302/150‑1 (MQ=255)

     cAATGTCACACAGGCAAAGATTGTGGCGGTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCCGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAactg                                                                                                                                                 <  2:1607779/150‑1 (MQ=255)

        tGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAactgact                                                                                                                                              <  1:1197624/150‑1 (MQ=255)

         gtaaaaCAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACTAAAAATCTCAGAATTCTCTTTGGCAACGAGATAATTTAACAGTTGATGATGAAACGAAATAGTCATCTTGGTTCTTTAATGCCAGGCAGTTGCTTAactgactg                                                                                                                                             <  1:1018469/147‑1 (MQ=255)

         gTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAactgactg                                                                                                                                             <  1:1023498/150‑1 (MQ=255)

         gTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAactgactg                                                                                                                                             >  2:546238/1‑150 (MQ=255)

           cAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGc                                                                                                                                           <  2:871051/150‑1 (MQ=255)

            aaaCAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTGCTTAACTGACTGGca                                                                                                                                          <  2:1431242/150‑2 (MQ=255)

            aaaCAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGct                                                                                                                                          >  1:653349/1‑150 (MQ=255)

               cAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCtt                                                                                                                                       <  2:1439956/150‑1 (MQ=255)

                 gTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTGCTTAACTGACTGGcattaca                                                                                                                                     <  1:892270/150‑7 (MQ=255)

                     aaGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCGAGTCAGTTAAGCAACTGACTGGCTCTTTTTCgg                                                                                                                                 <  1:851898/150‑1 (MQ=255)

                          tgtgGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCtg                                                                                                                            <  2:358052/150‑1 (MQ=255)

                              gCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTggg                                                                                                                        <  1:1580237/150‑1 (MQ=255)

                                      ggTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTcc                                                                                                                >  2:636222/1‑150 (MQ=255)

                                           gTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGc                                                                                                           <  1:1631970/150‑1 (MQ=255)

                                            gcTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGcc                                                                                                          <  2:1945031/149‑1 (MQ=255)

                                             cTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCct                                                                                                         >  1:1867440/1‑150 (MQ=255)

                                              ttGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCctc                                                                                                        <  2:1286610/150‑1 (MQ=255)

                                                 gggACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTcc                                                                                                     >  2:826231/1‑150 (MQ=255)

                                                  ggACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCATGGATCTGTAATGCCAGTCAGTTAAGCAACTTACTGGCTCTATTTCGGGGCTGTGGGGTATTTCAAGGGCCTCTCCt                                                                                                    >  1:1704040/1‑150 (MQ=255)

                                                     cAAAAAAGCTCAGTATTCTCGTTGGCAACCAGCTAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGATCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTa                                                                                                 >  1:84534/1‑150 (MQ=255)

                                                      aaaaaaGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTAc                                                                                                <  1:1985410/150‑1 (MQ=255)

                                                       aaaaaGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGAATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGCCTGGCTCTTTTTCGGGGCTGTGGGGTATTTACAGGTCCTCTCCTTTAcc                                                                                               >  1:1253242/1‑150 (MQ=255)

                                                               cAGTATTCTCGTTTGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAATCAACTGACTGGCTCTTTTTCGTGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACAACTCTCgg                                                                                       >  2:741729/1‑150 (MQ=255)

                                                               cAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCgg                                                                                       >  1:126193/1‑150 (MQ=255)

                                                                aGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGTCCCTCTCCTTTACCACTCTCggg                                                                                      >  2:1614179/1‑150 (MQ=255)

                                                                 gTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGa                                                                                     >  1:1901011/1‑150 (MQ=255)

                                                                    ttCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAgg                                                                                  >  2:1411077/1‑150 (MQ=255)

                                                                          ttGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCttt                                                                            >  2:627379/1‑150 (MQ=255)

                                                                           tGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTc                                                                           <  2:680129/150‑1 (MQ=255)

                                                                           tGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTc                                                                           <  2:928604/150‑1 (MQ=255)

                                                                           tGGCAAACAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTc                                                                           <  2:149160/150‑1 (MQ=255)

                                                                            ggCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTcc                                                                          <  2:1899120/150‑1 (MQ=255)

                                                                            ggCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTcc                                                                          >  2:993052/1‑150 (MQ=255)

                                                                             gCAACCAGATAATTTAACAGTTGATGATGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTATCATTTACCACTCTCGGGAATGCACTTTcca                                                                         >  1:814524/1‑149 (MQ=255)

                                                                                aacAGATAATTTAACAGTTTATGCTGAAACTAAATTGGCATCGTGGTTCTGTAATGCCAGTCAGATAACGAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGTCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCcttc                                                                      <  1:268042/148‑1 (MQ=255)

                                                                                aCCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCTGGAAGGCCCTTTCCctta                                                                      >  1:914262/1‑149 (MQ=255)

                                                                                aCCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCcttc                                                                      <  1:1662105/150‑1 (MQ=255)

                                                                                aCCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCGCCTTTACCACTCTCGGGAAGGCCCTTTCCcttg                                                                      >  1:780536/1‑149 (MQ=255)

                                                                                aCCAGATAATATAACAGTTGATGCTGAAACGACATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCCACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCcttc                                                                      <  1:256936/150‑1 (MQ=255)

                                                                                  aaGATAAATTACCAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTACCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCcttctt                                                                    <  2:1322235/149‑1 (MQ=255)

                                                                                   aGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTg                                                                   >  2:1797124/1‑150 (MQ=255)

                                                                                     aTAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTc                                                                 >  1:1141335/1‑150 (MQ=255)

                                                                                       aaTTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCgg                                                               <  2:1537663/150‑1 (MQ=255)

                                                                                             aCAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAAttt                                                         <  2:234812/150‑1 (MQ=255)

                                                                                              cAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCTACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTc                                                        <  1:1820258/150‑1 (MQ=255)

                                                                                              cAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTTGGTAATTTc                                                        <  1:1632361/150‑1 (MQ=255)

                                                                                              cAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAAttta                                                        >  1:1439644/1‑149 (MQ=255)

                                                                                              cAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTc                                                        >  1:1094532/1‑150 (MQ=255)

                                                                                              cAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTAGGTAAttta                                                        >  1:1814709/1‑149 (MQ=255)

                                                                                               aGTTGATGCTGAAACGAAATATGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAAATGACTGGCTCTTTTTCCGGGATGTGGGGTATTTCCAGGGCCTCTCCTTTACCAATCTCGTGAAGGCCCTTTCACTTCTTGTCGGTAATTTca                                                       >  2:799917/1‑150 (MQ=255)

                                                                                               aGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTca                                                       >  2:821699/1‑150 (MQ=255)

                                                                                               aGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTca                                                       >  2:1763792/1‑150 (MQ=255)

                                                                                                   gATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAg                                                   <  2:1597521/150‑1 (MQ=255)

                                                                                                    aTGCTGAAACGAAATAGGCATCTAGGTTCTGTAATCCCAGTCAGTTAATCAACTGACTGGCTCTATTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTATCGCGAACGCCCTTTCCATACTTGTCGGTAATTTCACAAGt                                                  >  1:166531/1‑150 (MQ=255)

                                                                                                      gCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGttt                                                >  2:875879/1‑149 (MQ=255)

                                                                                                       cTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACAACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGt                                               >  1:496630/1‑150 (MQ=255)

                                                                                                        tGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTc                                              >  1:493302/1‑150 (MQ=255)

                                                                                                         taaaCGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTACTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTcc                                             <  1:920910/149‑1 (MQ=255)

                                                                                                         gAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTcc                                             >  1:878642/1‑150 (MQ=255)

                                                                                                          aaaCGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAATCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTccc                                            <  2:2196/150‑1 (MQ=255)

                                                                                                          aaaCGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTccc                                            <  1:440879/150‑1 (MQ=255)

                                                                                                            aCGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCAt                                          <  1:77846/150‑1 (MQ=255)

                                                                                                              gAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATAc                                        >  1:1796083/1‑150 (MQ=255)

                                                                                                                 aTAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTg                                     <  1:1614489/150‑1 (MQ=255)

                                                                                                                  tAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGc                                    <  2:936593/150‑1 (MQ=255)

                                                                                                                   aGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCa                                   <  1:205875/150‑1 (MQ=255)

                                                                                                                    ggCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCaa                                  >  2:930476/1‑150 (MQ=255)

                                                                                                                    ggCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCaa                                  >  2:1021026/1‑150 (MQ=255)

                                                                                                                     gCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGTAAg                                 <  1:1387045/150‑1 (MQ=255)

                                                                                                                     gCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCGCGTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACACTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAg                                 <  1:1387048/150‑1 (MQ=255)

                                                                                                                       aTCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGAt                               >  1:1798563/1‑150 (MQ=255)

                                                                                                                       aTCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCATTTTCCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAAATTCACAAGTTGTAACATACATGCAAGAt                               >  1:495975/1‑150 (MQ=255)

                                                                                                                           tGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATcgcg                           <  2:653349/150‑1 (MQ=255)

                                                                                                                            ggTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCgcgc                          <  1:1251754/150‑1 (MQ=255)

                                                                                                                             gTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCa                         <  1:1883624/150‑1 (MQ=255)

                                                                                                                              ttCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATAGCGCAt                        >  1:1471524/1‑150 (MQ=255)

                                                                                                                                cTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACATGTTGTCCCATACTTGCAAGATCGCGCATCa                      >  2:1517559/1‑150 (MQ=255)

                                                                                                                                     gagCCAGTCAGTTGCTTAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCGCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATCCTTGCCAGATCGCGCATCCGCTcc                 >  1:1792672/3‑150 (MQ=255)

                                                                                                                                     aTGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTcc                 <  1:211849/150‑1 (MQ=255)

                                                                                                                                         cagtcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGta             >  1:1397402/1‑150 (MQ=255)

                                                                                                                                           gtcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGtata           <  1:658468/150‑1 (MQ=255)

                                                                                                                                           gtcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGtata           <  1:1151625/150‑1 (MQ=255)

                                                                                                                                            tcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATAc          <  2:1973697/150‑1 (MQ=255)

                                                                                                                                            tcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATAc          <  1:546238/150‑1 (MQ=255)

                                                                                                                                             cagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACg         >  2:1163715/1‑150 (MQ=255)

                                                                                                                                              agtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTATTTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCATCTCCGGTATACGt        >  2:1832992/1‑150 (MQ=255)

                                                                                                                                                ttAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTcc      >  2:1675033/1‑150 (MQ=255)

                                                                                                                                                ttAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTAACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATAAGTcc      >  1:727887/1‑150 (MQ=255)

                                                                                                                                                gtAAGCAACTGACTGGCTCATTTTCGGGGCTGTGGGGTATTTCAAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCGTTCCCTTCTTGTCGGTAATATCACAAGTTCACCCATACGTCCAAGATCGCGCATCAGCTCCGGTATACCTcc      >  1:820240/2‑150 (MQ=255)

                                                                                                                                                 tAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTccc     <  1:231288/150‑1 (MQ=255)

                                                                                                                                                   aGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCgg   <  1:1705374/150‑1 (MQ=255)

                                                                                                                                                    gCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGt  <  1:1740006/150‑1 (MQ=255)

CATGACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGT  >  NC_000913/4501959‑4502256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`