Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,519,311 A→G 5.4% intergenic (+87/+27) insB1 → / ← yjhU IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,519,3110AG 5.4% 453.4 / 11.1 148intergenic (+87/+27)insB1/yjhUIS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element
Reads supporting (aligned to +/- strand):  ref base A (66/73);  new base G (6/2);  total (72/76)
Fisher's exact test for biased strand distribution p-value = 1.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-01

Only 100 of 152 total aligned reads displayed.

AAATCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCAAG  >  NC_000913/4519162‑4519460
                                                                                                                                                     |                                                                                                                                                     
 aaTCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCatat                                                                                                                                                      >  1:1550518/1‑150 (MQ=255)
  aTCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATc                                                                                                                                                     <  2:633804/150‑1 (MQ=255)
   tCGGTTGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCt                                                                                                                                                    <  1:140222/150‑1 (MQ=255)
    cGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTa                                                                                                                                                   <  1:934935/150‑1 (MQ=255)
        ggAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATg                                                                                                                                               <  1:1625750/150‑1 (MQ=255)
          aGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATggg                                                                                                                                             >  2:1225538/1‑150 (MQ=255)
             tGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCa                                                                                                                                          >  2:1067786/1‑150 (MQ=255)
              gCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATGTAGATATGGGGCAg                                                                                                                                         <  2:477990/150‑1 (MQ=255)
              gCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAg                                                                                                                                         >  2:1295242/1‑150 (MQ=255)
               cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgt                                                                                                                                        >  1:1865974/1‑150 (MQ=255)
                 tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttg                                                                                                                                      >  1:1171261/1‑150 (MQ=255)
                 tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttg                                                                                                                                      >  2:1491405/1‑150 (MQ=255)
                    cAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgtgc                                                                                                                                   >  1:476638/1‑148 (MQ=255)
                     aaGGTCATCGTGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCa                                                                                                                                  <  1:1371622/150‑1 (MQ=255)
                     aaGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTACATACATTGTGGCTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCa                                                                                                                                  <  1:79761/150‑1 (MQ=255)
                      aGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCAt                                                                                                                                 <  1:854567/150‑1 (MQ=255)
                      aGGTCATCGGGAATTATCTGAACATAAAACACTATGAGTAAGTTGGCGTCATTACCGACCATGTTTATTTCATACATTTTGGGTATTGTTCTTTTTATCGCCGCTAAACAATAAAATCCTGACCCAAATCTACATGGGGCATTTGTTCAt                                                                                                                                 >  1:1231691/1‑150 (MQ=37)
                       ggTCATCGGGCATTATCTGAACATAAAACACTATCACTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCAtt                                                                                                                                >  2:1811548/1‑150 (MQ=255)
                         tCATCGTGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCt                                                                                                                              <  1:164268/150‑1 (MQ=255)
                          cATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtt                                                                                                                             >  2:1687562/1‑150 (MQ=255)
                            tCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                           <  1:1684497/150‑1 (MQ=255)
                            tCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                           <  2:887209/150‑1 (MQ=255)
                                 cATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtgt                                                                                                                      >  2:30721/1‑150 (MQ=255)
                                  aTTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGtgtg                                                                                                                     <  2:1571153/150‑1 (MQ=255)
                                  aTTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGtgtg                                                                                                                     <  1:1046933/150‑1 (MQ=255)
                                   ttATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTgg                                                                                                                    >  2:923760/1‑150 (MQ=255)
                                   ttATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTgg                                                                                                                    >  1:986570/1‑150 (MQ=255)
                                    tATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGt                                                                                                                   <  1:1949934/150‑1 (MQ=255)
                                        tGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAtt                                                                                                               <  2:1869717/150‑1 (MQ=255)
                                           aCATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTcac                                                                                                            <  2:1369356/150‑1 (MQ=255)
                                            cATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTcaca                                                                                                           <  2:908012/150‑1 (MQ=255)
                                            cATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTcaca                                                                                                           >  2:906771/1‑150 (MQ=255)
                                             aTAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCacac                                                                                                          >  1:1972489/1‑150 (MQ=255)
                                              tAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACg                                                                                                         >  2:1357070/1‑150 (MQ=255)
                                                aaaCACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGcc                                                                                                       <  1:115016/150‑1 (MQ=255)
                                                  acacTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAg                                                                                                     <  2:1553946/150‑1 (MQ=255)
                                                   cacTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGc                                                                                                    >  1:728974/1‑150 (MQ=255)
                                                   cacTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGc                                                                                                    <  1:634447/150‑1 (MQ=255)
                                                     cTATAAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCaa                                                                                                  >  2:1185745/1‑150 (MQ=255)
                                                      tATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCaaa                                                                                                 <  1:1067786/150‑1 (MQ=255)
                                                             aaGTTGGAGTCATTACCGACCATGTTTATTTCCTACATTGTTGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTg                                                                                          <  1:1789943/150‑1 (MQ=255)
                                                             aaGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTg                                                                                          <  2:887000/150‑1 (MQ=255)
                                                             aaGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTAATTCTTTTAGTGTGTTAATTCACACGCCAGCAAAAACTCTg                                                                                          >  2:1738353/1‑150 (MQ=255)
                                                                   gAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTc                                                                                    <  2:1764512/150‑1 (MQ=255)
                                                                     gTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACTCCAGCAAAAACTCTGCAGTTCCt                                                                                  >  1:1267583/1‑150 (MQ=255)
                                                                       cATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtc                                                                                <  2:1935364/150‑1 (MQ=255)
                                                                         ttACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcat                                                                              >  1:1033823/1‑150 (MQ=255)
                                                                            ccGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaa                                                                           <  1:676974/150‑1 (MQ=255)
                                                                                  aTGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCa                                                                     <  1:531855/150‑1 (MQ=255)
                                                                                    ttttATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCACCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAgg                                                                   <  1:36834/149‑1 (MQ=255)
                                                                                    gttaaattCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAACTCCTGCCCCAAATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCggg                                                                   <  2:271046/144‑1 (MQ=255)
                                                                                    gTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAgg                                                                   >  2:262839/1‑150 (MQ=255)
                                                                                    gTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAgg                                                                   <  1:570120/150‑1 (MQ=255)
                                                                                        aTTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTACg                                                               >  2:1786766/1‑150 (MQ=255)
                                                                                          ttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCATTAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTg                                                             <  1:60955/150‑1 (MQ=255)
                                                                                            cATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCACCATATCTACATGGGGAAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGAc                                                           >  1:298687/1‑150 (MQ=255)
                                                                                               aCATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACata                                                        <  1:704012/150‑1 (MQ=255)
                                                                                                  ttGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCATCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATAtcc                                                     <  1:991391/150‑1 (MQ=255)
                                                                                                    gtgGGTTTTGTTCTTATTATCGCCGCTAATAAATAAAATCCTGCCCCATATCTAGATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCAAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATAtcctc                                                   <  2:1078008/150‑1 (MQ=255)
                                                                                                     tgGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATAtcctcc                                                  <  1:407302/150‑1 (MQ=255)
                                                                                                       ggTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCca                                                <  2:1033823/150‑1 (MQ=255)
                                                                                                       ggTATTGTTCATATTATCGCCGCTAATCAATATAATCCTGCCCCATATCTACATTGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCca                                                <  1:438425/150‑1 (MQ=255)
                                                                                                            tGTTCTTATTATCGCCGCTAATCAATAAAATACTGACCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAgg                                           <  2:109988/150‑1 (MQ=255)
                                                                                                                cTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCGGGGCAc                                       >  2:1283637/1‑150 (MQ=255)
                                                                                                                cTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCAc                                       >  2:1408845/1‑150 (MQ=255)
                                                                                                                      atCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAATCTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCCCCCAACg                                 >  2:767701/1‑150 (MQ=255)
                                                                                                                       tCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGt                                >  1:1674548/1‑150 (MQ=255)
                                                                                                                        cgccgcTTATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAGCAATCAGGTCCGAGACATCTCCTCCCAGCAGGGCCCCCCACGtt                               >  2:1230080/1‑150 (MQ=255)
                                                                                                                        cgccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGtt                               >  1:1408958/1‑150 (MQ=255)
                                                                                                                        cgccgcTAATCAATAAAATCCTGCCCCATATCTACAAGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGtt                               >  2:899519/1‑150 (MQ=255)
                                                                                                                         gccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTg                              <  1:1990426/150‑1 (MQ=255)
                                                                                                                           cacTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCg                            <  1:595634/148‑1 (MQ=255)
                                                                                                                             cTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTc                          <  2:476638/150‑1 (MQ=255)
                                                                                                                               aaTCAATAAAATCCTGCCCCATATCAACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATAAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCAt                        >  1:544366/1‑150 (MQ=255)
                                                                                                                                aTCAATAAAATCCAGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATa                       <  1:828046/150‑1 (MQ=255)
                                                                                                                                  cAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGc                     <  1:761116/150‑1 (MQ=255)
                                                                                                                                  cAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTAATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTACTTCATCATCAATCAGGTCCGTGAAATATACTCCCAGCAGGGCACCCAACGATGCGTCATAGc                     >  2:66413/1‑150 (MQ=255)
                                                                                                                                       aacaacTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCAGTTCCTTAAGCAAAAATCAGGTACGTGAAATAACCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCctcat                >  2:385240/6‑148 (MQ=255)
                                                                                                                                       aaaTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGTCATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCtt                >  2:987742/1‑150 (MQ=255)
                                                                                                                                        acaacTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCttt               >  1:1076875/5‑150 (MQ=255)
                                                                                                                                          tgcTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc             <  1:247192/148‑1 (MQ=255)
                                                                                                                                          tCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc             <  2:1171261/150‑1 (MQ=255)
                                                                                                                                          tCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc             <  1:1615111/150‑1 (MQ=255)
                                                                                                                                          tCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc             <  1:467266/150‑1 (MQ=255)
                                                                                                                                          tCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTCGTAAATCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTGCCAGCAGGGCACCGAACGTTGCGTCATAGCCTCTTTcc             <  1:282658/150‑1 (MQ=255)
                                                                                                                                           acTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTccc            >  1:237217/2‑150 (MQ=255)
                                                                                                                                           acTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTccc            >  2:1659593/2‑150 (MQ=255)
                                                                                                                                            cTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcccc           >  2:823354/1‑150 (MQ=255)
                                                                                                                                             tGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTccccc          >  1:1520735/1‑149 (MQ=255)
                                                                                                                                             tGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCcca          >  2:766980/1‑150 (MQ=255)
                                                                                                                                             tGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCcca          <  2:728974/150‑1 (MQ=255)
                                                                                                                                              gCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccac         >  1:868122/1‑150 (MQ=255)
                                                                                                                                              gCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccac         <  2:740843/150‑1 (MQ=255)
                                                                                                                                                cccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccaccc       >  2:1985920/1‑149 (MQ=255)
                                                                                                                                                cccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacca       >  2:852964/1‑150 (MQ=255)
                                                                                                                                                cccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTACGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccaccc       >  1:1348237/1‑149 (MQ=255)
                                                                                                                                                 ccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCAAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccaccac      >  2:783083/1‑149 (MQ=255)
                                                                                                                                                  cATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGc     >  1:1862159/1‑150 (MQ=255)
                                                                                                                                                    tatCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCaa   >  2:851870/1‑150 (MQ=255)
                                                                                                                                                     atCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCAAg  <  1:478518/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAATCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCAAG  >  NC_000913/4519162‑4519460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: