Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,537,640 T→G 5.2% intergenic (‑46/+19) nanS ← / ← nanM 9‑O‑acetyl N‑acetylneuraminic acid esterase/N‑acetylneuraminic acid mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,537,6400TG 5.2% 405.1 / 9.2 134intergenic (‑46/+19)nanS/nanM9‑O‑acetyl N‑acetylneuraminic acid esterase/N‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base T (56/71);  new base G (2/5);  total (58/76)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

Only 100 of 135 total aligned reads displayed.

TCTTCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAGG  >  NC_000913/4537491‑4537786
                                                                                                                                                     |                                                                                                                                                  
  ttCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTa                                                                                                                                                  >  1:1694118/1‑150 (MQ=255)
    cccTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTAcc                                                                                                                                                >  2:1309810/1‑150 (MQ=255)
      cTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgg                                                                                                                                              <  1:225665/150‑1 (MQ=255)
        gTCCGGTAATGGCAGTCCTTCGCCTTACGCCATGGCATTAGACTGACCAGCAAAGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                            <  2:1438375/150‑1 (MQ=255)
        gTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCgggg                                                                                                                                            <  1:1778166/150‑1 (MQ=255)
         tCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACTGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGc                                                                                                                                           <  2:804835/150‑1 (MQ=255)
          ccGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGcc                                                                                                                                          <  1:1365802/150‑1 (MQ=255)
           cGGTAATGGCAGTCCTTCGCCATACTCCATGGCATGAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCATGGCCCCTTTACCGGGGccc                                                                                                                                         <  1:1465005/150‑1 (MQ=255)
           cGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCGGTAAAGGGGccc                                                                                                                                         <  2:742287/150‑1 (MQ=255)
            ggTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCt                                                                                                                                        >  2:1011288/1‑150 (MQ=255)
            ggTAATGGCAGTCCTTCGCCATACACCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCt                                                                                                                                        <  1:1143622/150‑1 (MQ=255)
             gTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTg                                                                                                                                       <  2:1372631/150‑1 (MQ=255)
              tAATGGCAGTCCTTCGCCATACGCGATGGCATTAGACTGAGCAGCTACGGTAAGAAGATAGTTATAATCGGGGGATATTTTTGCGTTCATATAAATTTCGCGTAATTTAAATGACAAATTGATAGCAGGGCCCCGGTAAAGGGGCCCTgc                                                                                                                                      <  2:893265/150‑2 (MQ=255)
              tAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGa                                                                                                                                      <  1:502947/150‑1 (MQ=255)
              tAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGa                                                                                                                                      <  1:1366268/150‑1 (MQ=255)
              tAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCGGTAAAGGGGCCCTgc                                                                                                                                      <  2:901143/150‑2 (MQ=255)
              tAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCGGTAAAGGGGCCCTgc                                                                                                                                      <  2:1081302/150‑2 (MQ=255)
               aaTGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATACTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCGGTAAAGGGGCCCTgct                                                                                                                                     <  2:179452/150‑3 (MQ=255)
                 tGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGc                                                                                                                                   >  2:1840482/1‑150 (MQ=255)
                 tGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACAAAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGc                                                                                                                                   <  1:730036/150‑1 (MQ=255)
                  ggCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCg                                                                                                                                  <  2:888819/150‑1 (MQ=255)
                  ggCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCg                                                                                                                                  <  1:26072/150‑1 (MQ=255)
                    cAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGtt                                                                                                                                >  1:260369/1‑150 (MQ=255)
                      gTCCTTCGCCATAAGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAg                                                                                                                              <  2:1179072/150‑1 (MQ=255)
                         cTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGttt                                                                                                                           >  1:1165171/1‑150 (MQ=255)
                         cTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGttt                                                                                                                           <  2:1476920/150‑1 (MQ=255)
                           tCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGttttt                                                                                                                         >  1:213339/1‑149 (MQ=255)
                           tCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTg                                                                                                                         <  2:1778273/150‑1 (MQ=255)
                            cGCCATACGCCATGTCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAATTGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGt                                                                                                                        <  1:2009668/150‑1 (MQ=255)
                             gcaaTACGCCATGTCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTa                                                                                                                       <  2:592050/147‑1 (MQ=255)
                              ccATACGCCATGGCATTTGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTAc                                                                                                                      >  2:1120611/1‑150 (MQ=255)
                              ccATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTCTTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTAc                                                                                                                      <  1:277915/150‑1 (MQ=255)
                               cATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACt                                                                                                                     >  1:680767/1‑150 (MQ=255)
                                  aCGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACtgtg                                                                                                                  <  2:520366/150‑1 (MQ=255)
                                   cGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGa                                                                                                                 >  1:826195/1‑150 (MQ=255)
                                    gCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGAc                                                                                                                >  1:562641/1‑150 (MQ=255)
                                     ccATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACt                                                                                                               <  2:1331896/150‑1 (MQ=255)
                                     ccATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACt                                                                                                               <  2:45859/150‑1 (MQ=255)
                                        tGGCATTAGACTGACCAGCAAAGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTtta                                                                                                            <  1:570076/150‑1 (MQ=255)
                                            aTTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTtattat                                                                                                        >  1:1012167/1‑150 (MQ=255)
                                              tAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCt                                                                                                     <  1:912755/150‑1 (MQ=255)
                                                gACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCtt                                                                                                    <  2:815294/150‑1 (MQ=255)
                                                 aCTGACAAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACGGTGACTTTATTATCCTTc                                                                                                   >  2:490763/1‑150 (MQ=255)
                                                  cTGCCCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAAATTCGCGTAATTGAAATGACAAATTGATATCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTTGTTTTGTACTGTGACTTTATTATCCTTca                                                                                                  <  2:129590/150‑1 (MQ=255)
                                                  cTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTca                                                                                                  >  2:6022/1‑150 (MQ=255)
                                                  cTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTca                                                                                                  >  1:413639/1‑150 (MQ=255)
                                                  cTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTca                                                                                                  >  1:1197957/1‑150 (MQ=255)
                                                    gACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTcaca                                                                                                >  2:1201785/1‑150 (MQ=255)
                                                       cAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTg                                                                                             >  2:1574601/1‑150 (MQ=255)
                                                        aGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGa                                                                                            >  2:1675571/1‑150 (MQ=255)
                                                        aGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGa                                                                                            >  1:1520831/1‑150 (MQ=255)
                                                         gCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGAt                                                                                           >  1:1980291/1‑150 (MQ=255)
                                                              ggTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCaaaa                                                                                      >  1:1736696/1‑150 (MQ=255)
                                                               gTACGAACATAGTAAAAATCTGGCGCTATTATAGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAAc                                                                                     <  2:289515/150‑1 (MQ=255)
                                                               gTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAAc                                                                                     <  1:1068429/150‑1 (MQ=255)
                                                                  aGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGa                                                                                  >  1:551502/1‑150 (MQ=255)
                                                                      cATAGTAATAATCGGGCGATATTATTGCGTTCATAAAAATTTCGCGTAAATGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCATGAAGCGTTAGTTTTGTACTGTGACTTTATTATCATTCACAGTGATCAAAACTGAATcc                                                                              >  2:323042/1‑150 (MQ=255)
                                                                      aaTAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATcc                                                                              <  1:1737940/149‑1 (MQ=255)
                                                                       aTAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCg                                                                             <  1:1120611/150‑1 (MQ=255)
                                                                       aTAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAAATGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCg                                                                             <  2:1512964/150‑1 (MQ=255)
                                                                         aGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTc                                                                           <  2:232771/150‑1 (MQ=255)
                                                                         aGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTc                                                                           <  1:1316966/150‑1 (MQ=255)
                                                                          gTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCa                                                                          >  2:774342/1‑150 (MQ=255)
                                                                             ataatCGGGCGATATTATTGCTTTCATATAAATTTCTCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACAGGGGCCCTGAAGCGTTAGTTTTGTAATGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCAGTCAcag                                                                       >  2:207675/1‑148 (MQ=255)
                                                                                       gATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTgccgcc                                                             <  2:955009/150‑1 (MQ=255)
                                                                                        atatTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCt                                                            >  1:1938616/1‑150 (MQ=255)
                                                                                          attattGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGc                                                          <  2:629266/150‑1 (MQ=255)
                                                                                           ttattGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCa                                                         <  1:1056862/150‑1 (MQ=255)
                                                                                             attGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGt                                                       <  1:1916229/150‑1 (MQ=255)
                                                                                             attGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGt                                                       <  1:386704/150‑1 (MQ=255)
                                                                                              ttGCGTTCATATAAATTTCTCTAAATTGAAATGACAAATTGATAGCAGCGCCCCTTTACCGGGGCCCTGAAGCGATAGTGTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGGCACCGCTTTGCCGCCTGCAGtt                                                      <  2:139418/150‑1 (MQ=255)
                                                                                              ttGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGtt                                                      <  1:1672525/150‑1 (MQ=255)
                                                                                              ttGCGTTCATATAAATTTCGCGTAATTGAAAAGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGtt                                                      <  1:867185/150‑1 (MQ=255)
                                                                                                 cGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCa                                                   >  1:513153/1‑150 (MQ=255)
                                                                                                   ttCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAAATGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCAcc                                                 >  1:1322361/1‑150 (MQ=255)
                                                                                                    tCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCg                                                <  2:945530/150‑1 (MQ=255)
                                                                                                    tCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCg                                                <  1:1840482/150‑1 (MQ=255)
                                                                                                    tCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCg                                                <  1:976641/150‑1 (MQ=255)
                                                                                                     cATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGc                                               >  2:1150856/1‑150 (MQ=255)
                                                                                                      atatAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGCCTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGcc                                              >  1:367609/1‑150 (MQ=255)
                                                                                                       tataAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGAACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCa                                             >  1:1853634/1‑150 (MQ=255)
                                                                                                        ataAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCaa                                            >  2:1648497/1‑150 (MQ=255)
                                                                                                            aTTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCaataat                                        >  1:1551320/1‑150 (MQ=255)
                                                                                                             tttCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATc                                       <  1:1206750/150‑1 (MQ=255)
                                                                                                              ttCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCa                                      <  2:1992258/150‑1 (MQ=255)
                                                                                                                cgcgTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAAt                                    >  2:1708755/1‑150 (MQ=255)
                                                                                                                   gTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGa                                 <  2:1007537/150‑1 (MQ=255)
                                                                                                                     aaTTGAAATGTCAAATTTTTAGCAGGGCCCCTTTCCCGGCGCCCTGAAGCGTTAGTTTTGTCCTGTGACTTTATTCTCCTTCACAGTGATCAAAACTGAATCCGTCACGGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACt                               <  2:112951/150‑1 (MQ=255)
                                                                                                                         gAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGGTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACtatta                           >  2:1542774/1‑150 (MQ=255)
                                                                                                                         gAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACtatta                           <  2:1520831/150‑1 (MQ=255)
                                                                                                                             tGACTAATTGATAGCATGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTcc                       <  1:1515669/150‑1 (MQ=255)
                                                                                                                             tGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTcc                       >  1:1204122/1‑150 (MQ=255)
                                                                                                                                cAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAgg                    >  1:1807576/1‑150 (MQ=255)
                                                                                                                                    ttGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCaa                >  2:655277/1‑150 (MQ=255)
                                                                                                                                        cttcAGGGCCCCGGTAAAGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGAt            >  1:694711/4‑150 (MQ=255)
                                                                                                                                            aGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGGATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTc        <  2:656088/150‑1 (MQ=255)
                                                                                                                                                 cccTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAg   <  1:1330786/150‑1 (MQ=255)
                                                                                                                                                 cccTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACAGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAg   >  2:1452801/1‑150 (MQ=255)
                                                                                                                                                  ccTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTAACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAgg  >  2:1360497/1‑150 (MQ=255)
                                                                                                                                                  ccTTTACCGGGGCCCGGAAGCGTTAGTTTTGTACTGGGACTTTCTTATCCTTCACAGGGAGCAACACGGAATCCGTCACCGCCTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGCTACTCCGTAgg  <  2:1442260/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TCTTCCCTGTCCGGTAATGGCAGTCCTTCGCCATACGCCATGGCATTAGACTGACCAGCAACGGTAAGAACATAGTAATAATCGGGCGATATTATTGCGTTCATATAAATTTCGCGTAATTGAAATGACAAATTGATAGCAGGGCCCCTTTACCGGGGCCCTGAAGCGTTAGTTTTGTACTGTGACTTTATTATCCTTCACAGTGATCAAAACTGAATCCGTCACCGCTTTGCCGCCTGCAGTTTCACCGCCAATAATCAATAGACTATTATTCCAGGGCAATGATACTCCGTAGG  >  NC_000913/4537491‑4537786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: