Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 481,228 C→A 7.2% intergenic (‑520/+26) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913481,2280CA 7.2% 307.3 / 8.1 104intergenic (‑520/+26)tomB/acrBHha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (51/45);  new base A (4/4);  total (55/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCAT  >  NC_000913/481080‑481373
                                                                                                                                                    |                                                                                                                                                 
tATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAcg                                                                                                                                                  >  2:1055728/1‑150 (MQ=255)
   tAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGTTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCgg                                                                                                                                               >  1:2006234/1‑150 (MQ=255)
   tAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCgg                                                                                                                                               >  2:1699596/1‑150 (MQ=255)
   tAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCgg                                                                                                                                               >  2:417784/1‑150 (MQ=255)
    aGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGc                                                                                                                                              <  2:595420/150‑1 (MQ=255)
     gcgcTACTTAATATAAATTAACTATCAAGCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTCCTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGcc                                                                                                                                             <  1:284395/150‑1 (MQ=255)
     gcgcTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGTTTATGCATAAAAAAGGCCGCTTACGCGGcc                                                                                                                                             >  1:1760172/1‑150 (MQ=255)
     gcgcTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGcc                                                                                                                                             >  1:1464005/1‑150 (MQ=255)
       gcTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGGTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCtt                                                                                                                                           <  1:183098/150‑1 (MQ=255)
          aCTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGt                                                                                                                                        >  2:1460026/1‑150 (MQ=255)
          aCTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttt                                                                                                                                        <  2:1944015/150‑4 (MQ=255)
              aaTATAAATTAACTAACAATCAGTATTTTTTTTCAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAtt                                                                                                                                    <  1:381757/150‑1 (MQ=255)
              aaTATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttttttatg                                                                                                                                    <  1:1607006/150‑8 (MQ=255)
                 ataAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTaca                                                                                                                                 >  1:1860634/1‑150 (MQ=255)
                  taAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCTGCCTTAGTGATTacac                                                                                                                                >  2:1113763/1‑150 (MQ=255)
                    aaTTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGt                                                                                                                              <  1:14603/150‑1 (MQ=255)
                      ttAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTACTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGttt                                                                                                                            >  1:1763401/1‑149 (MQ=255)
                      ttAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAAAACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTg                                                                                                                            <  2:1536123/150‑1 (MQ=255)
                      ttAACAAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTg                                                                                                                            <  1:1008956/150‑1 (MQ=255)
                         actaacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTAt                                                                                                                         >  2:1801620/1‑150 (MQ=255)
                          ctaacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATc                                                                                                                        <  2:801572/150‑1 (MQ=255)
                          ctaacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGGTTTCGTATGAGATCCTGAGATGGTGGTTAATTTACGCCGTTAGGTTCGTAGGGTATGCTTTAAAAAGGCCGCTTACGCGGCCTTAGTGATTAAACGATGTATc                                                                                                                        >  2:69936/1‑150 (MQ=255)
                           taacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCa                                                                                                                       >  1:846759/1‑150 (MQ=255)
                           aaacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCa                                                                                                                       <  2:1215651/149‑1 (MQ=255)
                            aacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCaa                                                                                                                      >  1:1173233/1‑150 (MQ=255)
                                    ttATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATc                                                                                                              <  1:1173371/149‑1 (MQ=255)
                                    gTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATc                                                                                                              >  2:1289739/1‑150 (MQ=255)
                                    gTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATc                                                                                                              <  1:1343695/150‑1 (MQ=255)
                                      tttttttATGAATTTTATCCGTTGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGa                                                                                                            <  1:145015/149‑1 (MQ=255)
                                         ttttATGAATTTTATCCGTGGTTAATACTGGTTATCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAg                                                                                                         <  1:395422/150‑1 (MQ=255)
                                         ttttATGAATTTTATCCGTGGTTAATACTGCTTTTCGTATGAGATCCTGACTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCAAAAAAAAGGCCGAATACGCCGCCTTAGTGATTACAAGTTGAATCAATGCTGAACGACAg                                                                                                         >  1:903386/1‑150 (MQ=255)
                                             aTGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATg                                                                                                     >  2:1945307/1‑150 (MQ=255)
                                              tGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATgg                                                                                                    <  1:1328854/150‑1 (MQ=255)
                                               gAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGc                                                                                                   >  1:487818/1‑150 (MQ=255)
                                                aaTTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCt                                                                                                  >  1:1134600/1‑150 (MQ=255)
                                                 aTTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTTGGTTATGCATAAAAAAGGCCGCTTTCGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtg                                                                                                 <  1:516972/150‑1 (MQ=255)
                                                 aTTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTTTATCAATGATGATCGACAGTATGGCtg                                                                                                 <  1:1814525/150‑1 (MQ=255)
                                                 aTTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtg                                                                                                 <  2:1790720/150‑1 (MQ=255)
                                                  ttttATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtgt                                                                                                <  1:783631/150‑1 (MQ=255)
                                                      aTCCGTGGTTAATACTGGTTTTCGTATGAGATCCTTAGTTGGTTGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGAGGACTTTGTTATTACACGTTGTATCAATTATGATCTACAGTATTGGTGTtctc                                                                                            <  2:1929392/150‑1 (MQ=255)
                                                         cGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGCGCTCGat                                                                                         <  2:123239/150‑1 (MQ=255)
                                                              ttAATACTGGTTTTGGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCTTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCtt                                                                                    <  1:363891/150‑1 (MQ=255)
                                                                  tACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTAACAATGATGATCGACAGTATGGCTGTGATCGATATCTTCAtt                                                                                >  1:67822/1‑150 (MQ=255)
                                                                   gcTGGATTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCAtaa                                                                               >  1:1978989/2‑148 (MQ=255)
                                                                       gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGc                                                                           <  1:636875/150‑1 (MQ=255)
                                                                       gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGAGCTCGATATCTTCATTCATGc                                                                           >  2:1232474/1‑150 (MQ=255)
                                                                        ttttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCg                                                                          <  2:1986890/150‑1 (MQ=255)
                                                                         tttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTACTGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCgg                                                                         <  2:339449/150‑1 (MQ=255)
                                                                            cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTa                                                                      <  1:640253/150‑1 (MQ=255)
                                                                                 gagaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcg                                                                 >  1:1739975/1‑150 (MQ=255)
                                                                                 gagaTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcg                                                                 >  1:121597/1‑150 (MQ=255)
                                                                                    aTCCTGAGTTGGTGGGTCACTTAATCGTTAATGTTCGTAGGTTCTGCATAAAAAAGGCCGCGTACGCGGCCTTAGTGATTCCACGTTGTATCACTGATGATCGCCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcg                                                              <  1:223932/150‑1 (MQ=255)
                                                                                      ccTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc                                                            <  1:942573/150‑1 (MQ=255)
                                                                                      ccTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc                                                            <  2:1191289/150‑1 (MQ=255)
                                                                                         gAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGaa                                                         >  2:872800/1‑150 (MQ=255)
                                                                                         gAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGaa                                                         <  1:1055728/150‑1 (MQ=255)
                                                                                          aGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAac                                                        <  1:463824/150‑1 (MQ=255)
                                                                                          aGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAac                                                        <  1:1699596/150‑1 (MQ=255)
                                                                                           gTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTTTCAATGATGATCGTCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCTAaca                                                       >  1:645920/1‑149 (MQ=255)
                                                                                           gTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAacc                                                       >  2:1657976/1‑150 (MQ=255)
                                                                                              ggtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAaccacc                                                    <  1:1104319/150‑1 (MQ=255)
                                                                                              ggtggtTCAATTACTCCTTAATGTTCGTAGGTAATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAaccacc                                                    <  1:365807/150‑1 (MQ=255)
                                                                                                     cAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGa                                             >  1:33333/1‑150 (MQ=255)
                                                                                                      aaTTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGaa                                            >  2:1727494/1‑150 (MQ=255)
                                                                                                             ccTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGa                                     >  1:683010/1‑150 (MQ=255)
                                                                                                              cTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCTGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGaa                                    >  1:1295080/1‑150 (MQ=255)
                                                                                                                tAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACg                                  >  2:460899/1‑150 (MQ=255)
                                                                                                                tAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACg                                  >  1:248541/1‑150 (MQ=255)
                                                                                                                   tGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaag                               <  2:1764661/150‑1 (MQ=255)
                                                                                                                   tGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAAACACCACAAAGAATACCGGAACgaag                               >  2:479634/1‑150 (MQ=255)
                                                                                                                     ttCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaagaa                             >  2:952901/1‑150 (MQ=255)
                                                                                                                          aGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTg                        <  1:838210/150‑1 (MQ=255)
                                                                                                                              tATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAg                    >  1:1358774/1‑150 (MQ=255)
                                                                                                                              tATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAg                    <  1:1945307/150‑1 (MQ=255)
                                                                                                                                tGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTa                  >  1:806250/1‑150 (MQ=255)
                                                                                                                                  cATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTAcc                >  2:1178331/1‑150 (MQ=255)
                                                                                                                                   aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCCTTCTTGCGGCTAAAGCGGCGGCGAAACACCACAAAGAATACCGTAACGCAGAAGATTGCAAGTACCg               >  2:1790146/1‑150 (MQ=255)
                                                                                                                                   aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGATGACCGGAACGAATAAGATTGCCAGTACCg               >  1:1352247/1‑150 (MQ=255)
                                                                                                                                   aTAAAAAAGGCCGCATACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATAGACAGAAAGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAACCGGCGGCGAACCACCACAAAGAATACCGGAACCAAGAAGATTGCCAGTAcac               >  2:1551866/1‑148 (MQ=255)
                                                                                                                                    tAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt              >  1:356285/1‑150 (MQ=255)
                                                                                                                                    tAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGt              >  1:1135787/1‑150 (MQ=255)
                                                                                                                                     aaaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGCCAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt             >  2:1702660/1‑150 (MQ=255)
                                                                                                                                     aaaaaaGGCCGCTTACGCGGCCGTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGACCCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt             >  1:1293756/1‑150 (MQ=255)
                                                                                                                                      actaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg            >  2:278187/4‑150 (MQ=255)
                                                                                                                                      actaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg            >  1:766786/4‑150 (MQ=255)
                                                                                                                                      aaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg            >  2:1587364/1‑150 (MQ=255)
                                                                                                                                      aaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg            <  1:1328479/150‑1 (MQ=255)
                                                                                                                                      aaaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg            <  2:1469118/150‑1 (MQ=255)
                                                                                                                                       ctaaGGCCGCGTAAGCGGCCATAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGACAGTACCGTTGc           >  2:1208081/3‑150 (MQ=255)
                                                                                                                                         aaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCgg         <  2:2852/150‑1 (MQ=255)
                                                                                                                                           ggCCGCTTACGCGGCCTTAGTGATTCCACGTTGTATCAATGATGATCGACAGTATGGCTGGGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTc       <  2:1760172/150‑1 (MQ=255)
                                                                                                                                           ggCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACGACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTc       >  2:368442/1‑150 (MQ=255)
                                                                                                                                            gCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCa      <  1:186578/150‑1 (MQ=255)
                                                                                                                                            gCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCa      <  2:1860634/150‑1 (MQ=255)
                                                                                                                                            gCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCa      >  1:67896/1‑150 (MQ=255)
                                                                                                                                            gCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCa      <  1:1165977/150‑1 (MQ=255)
                                                                                                                                             ccGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCAc     <  1:235909/150‑1 (MQ=255)
                                                                                                                                             ccGCTTACGCGGCCTAAGTGATTACACGTTGTATCAATTATGATCGACAGTATGGCTGTGCGCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCAc     <  1:1301805/150‑1 (MQ=255)
                                                                                                                                               gCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCa   >  2:1402992/1‑150 (MQ=255)
                                                                                                                                                cTTACGAGGCATTAGTGATTACACGTTGTATCAATGATGATCGAAAGTATGGCTTTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAAACACCACAAAGAATACAGGAACTAAGAAGATTGCCATTACCGTTGCGGTCACAAt  >  2:1696699/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCAT  >  NC_000913/481080‑481373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑