Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,551,422 T→A 5.1% intergenic (‑126/‑214) gntP ← / → uxuA fructuronate transporter/mannonate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,551,4220TA 5.1% 471.9 / 8.0 153intergenic (‑126/‑214)gntP/uxuAfructuronate transporter/mannonate hydrolase
Reads supporting (aligned to +/- strand):  ref base T (74/71);  new base A (4/4);  total (78/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

AGAGAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGAT  >  NC_000913/4551275‑4551569
                                                                                                                                                   |                                                                                                                                                   
agagAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAttt                                                                                                                                                   >  1:347382/1‑150 (MQ=255)
  agaATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgc                                                                                                                                                 <  1:1197786/150‑1 (MQ=255)
   taaTGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgca                                                                                                                                                <  1:1649081/149‑1 (MQ=255)
   taaTGTTAAGCACATGCATAACGTTTTACCATACATGGTTGAACCGTTGTTATTTTGGGAGATATGTTATGTAAATTGGTCAAGCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgca                                                                                                                                                <  2:1350751/149‑1 (MQ=255)
   gaATGTTAAGCACATGCATAACGTTTTACGTTACCTGGTTGAACCGGTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCCGCGATTACCCTTTGCTGCAATTTgca                                                                                                                                                >  2:204487/1‑150 (MQ=255)
      tGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgcagca                                                                                                                                             >  1:981771/1‑150 (MQ=255)
      tGTTAAGCACATGCATAACGTTTTAACTTACCTGGTTGAACCGTTGTTATTTTGGGAGATAAGTTATGTAAATTGGACAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAAAAATAGATTACCCTTTGCTGCAATTTgcagca                                                                                                                                             >  2:623185/1‑150 (MQ=255)
         tAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAAAGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGcaaca                                                                                                                                          >  2:1775139/1‑150 (MQ=255)
           aGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaaggg                                                                                                                                        <  1:1276613/150‑5 (MQ=255)
           aGCACATGCATAACGTTTTACAATAACTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACAATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTAGCAGAaacaac                                                                                                                                        >  2:185391/1‑150 (MQ=255)
             cacaTGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCa                                                                                                                                      <  2:1946790/150‑1 (MQ=255)
                 tGCATAACGTTTTACCTTACCTGGTTGAACCGTTGATAATTTGGGCGATATGTTATGTAAATTGGACAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATGGATTACCCTTTGCTGAAATTTCCAGCAACACCCATgaa                                                                                                                                  >  1:374370/1‑149 (MQ=255)
                   cATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGagag                                                                                                                                >  1:447349/1‑150 (MQ=255)
                            ttaccttaccTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCt                                                                                                                       <  1:409796/150‑1 (MQ=255)
                               ccttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtgt                                                                                                                    >  1:1269039/1‑150 (MQ=255)
                                cttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTAAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtgtg                                                                                                                   <  2:1220568/150‑1 (MQ=255)
                                  tacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGAt                                                                                                                 >  1:1932579/1‑150 (MQ=255)
                                   acctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGAtg                                                                                                                >  1:1406232/1‑150 (MQ=255)
                                    cctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGAtgt                                                                                                               >  2:535404/1‑150 (MQ=255)
                                        ggtGAACCGTTCGTATGTGGGGCGTTATGTTATGTAAATAGGGCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATACCCATCGATTACCCTTTGCTTCAATTAGCAGCAACAACCATGAGAGTGAAAATCTTGTGATGTGGtt                                                                                                           <  2:1327703/148‑1 (MQ=255)
                                         ttGAACCGGTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTa                                                                                                          <  1:1658433/150‑1 (MQ=255)
                                           gAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTatc                                                                                                        >  2:1013898/1‑148 (MQ=255)
                                            aaCCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAAcc                                                                                                       <  2:55166/150‑1 (MQ=255)
                                              ccGTTGTTATTTTGGGCGATATGTTATGTTAATTGGTAAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCaa                                                                                                     <  1:643655/150‑1 (MQ=255)
                                              ccGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGATAGTGAAATTCTTGTGATGTGGTTAACCaa                                                                                                     >  1:1280781/1‑150 (MQ=255)
                                                  tgttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCTATTTGAAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTATCCAATTTc                                                                                                 >  1:212584/1‑150 (MQ=255)
                                                  tgttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTc                                                                                                 <  2:1921528/150‑1 (MQ=255)
                                                   tttATTTTTGGCGATATCTTATGTAAATTGTGCAACAATTGTTGCGATGAATGTCACATCCTCAGATCAATAACCATCGATTACCCTTTGATGCAATTTGCTGCAACATCAATGAGTGTGAAATTCTTGTGATGTTGTTAACCAATTTCa                                                                                                <  2:981317/149‑1 (MQ=255)
                                                   gttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCATCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCa                                                                                                <  1:535358/150‑1 (MQ=255)
                                                   gttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCa                                                                                                <  2:1299753/150‑1 (MQ=255)
                                                   gttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCa                                                                                                <  2:1898755/150‑1 (MQ=255)
                                                   gttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCa                                                                                                >  1:903984/1‑150 (MQ=255)
                                                    ttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAg                                                                                               >  1:5348/1‑150 (MQ=255)
                                                       ttttGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAAACAATTTCAGAAt                                                                                            >  2:1205471/1‑150 (MQ=255)
                                                          tGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCg                                                                                         >  2:41870/1‑150 (MQ=255)
                                                           ggtcGATATGATATGTAAATTTGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCATTAAGCATCGATTACCCTTTGCTGCAATTTGCAGCTTCAACCATGAGAGTGAAATTATTGTGATGTGGTTAACCAATTTCAGAATTCgg                                                                                        <  1:37487/147‑1 (MQ=255)
                                                            ggCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCggg                                                                                       <  2:618145/150‑1 (MQ=255)
                                                              cGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGtt                                                                                     <  1:500620/150‑1 (MQ=255)
                                                              cGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGtt                                                                                     <  1:1762910/150‑1 (MQ=255)
                                                              cGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGtt                                                                                     <  2:1355399/150‑1 (MQ=255)
                                                                 tatgttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGAc                                                                                  <  1:1570805/150‑1 (MQ=255)
                                                                 tatgttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGAc                                                                                  <  2:1405385/150‑1 (MQ=255)
                                                                  atgttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACa                                                                                 <  1:651871/150‑1 (MQ=255)
                                                                   tgttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTACCCAATTTCAGAATTCGGGTTGACAt                                                                                >  2:569033/1‑150 (MQ=255)
                                                                     ttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGt                                                                              >  2:16955/1‑150 (MQ=255)
                                                                       atgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCt                                                                            >  1:99655/1‑150 (MQ=255)
                                                                       atgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCt                                                                            >  1:933460/1‑150 (MQ=255)
                                                                          tAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTAc                                                                         >  1:1882503/1‑150 (MQ=255)
                                                                                ggTCAACCATTGTTGCGATGAATGTCACATCCTCGGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTATTGTGATGTGGTTAACCATATTCAGAATTCGGGTTGACATGGCTTACCAAAAg                                                                   >  2:294494/1‑150 (MQ=255)
                                                                                   cAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTa                                                                <  1:1680353/150‑1 (MQ=255)
                                                                                   cAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTa                                                                >  1:764665/1‑150 (MQ=255)
                                                                                   cAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTa                                                                >  2:31035/1‑150 (MQ=255)
                                                                                   cAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTa                                                                >  1:1565777/1‑150 (MQ=255)
                                                                                    aaCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTTATGTTGTTAACCAATTTCAGAATTCCTGTTTACATGTATTACCAAAAGGTAg                                                               >  2:1688019/1‑150 (MQ=255)
                                                                                     aCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGa                                                              >  2:1071244/1‑150 (MQ=255)
                                                                                          tgttgCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTta                                                         <  1:1716855/150‑1 (MQ=255)
                                                                                            ttgCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTtata                                                       <  1:1731583/150‑1 (MQ=255)
                                                                                            ttgCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTtata                                                       <  1:134705/150‑1 (MQ=255)
                                                                                            ttgCGATGAATGTCACATCCTCTGATCAAAAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTtata                                                       >  2:1449457/1‑150 (MQ=255)
                                                                                             tgCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATAc                                                      <  2:347382/150‑1 (MQ=255)
                                                                                                    aaTGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCAtct                                               >  1:1004349/1‑150 (MQ=255)
                                                                                                    aaTGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACAAATTTCAGAATTCGGGTTGACATGTCTTACAAAAAGGTAGAACTTATACTCCAtct                                               >  2:66860/1‑150 (MQ=255)
                                                                                                       gTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACAAAAAGGTAGAACTTATACGCCATCTCAt                                            >  2:706557/1‑150 (MQ=255)
                                                                                                        tctcaTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATc                                           <  1:1138228/147‑1 (MQ=255)
                                                                                                        tCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATc                                           >  2:591291/1‑150 (MQ=255)
                                                                                                           caTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGa                                        >  2:752798/1‑150 (MQ=255)
                                                                                                           caTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGa                                        >  2:782996/1‑150 (MQ=255)
                                                                                                             tCCTCTGATCAATACCCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGGGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATg                                      <  1:1009478/150‑1 (MQ=255)
                                                                                                             tCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATg                                      <  2:14075/150‑1 (MQ=255)
                                                                                                                tctGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGATATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCaa                                   >  2:691798/1‑150 (MQ=255)
                                                                                                                 ctGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAAATTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAAATTATACGCCATCTCATCCGATGCAAc                                  >  1:713149/1‑150 (MQ=255)
                                                                                                                  tGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAAcc                                 >  2:602072/1‑149 (MQ=255)
                                                                                                                   gATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGc                                >  2:1111516/1‑150 (MQ=255)
                                                                                                                   gATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGc                                >  1:1727381/1‑150 (MQ=255)
                                                                                                                     tCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGccc                              >  2:1498392/1‑149 (MQ=255)
                                                                                                                     tCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCa                              >  2:1132253/1‑150 (MQ=255)
                                                                                                                     tCAATAAACATCGAATACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTTAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACTCCATCTCATCCGATGCAACGCCa                              <  2:360426/150‑1 (MQ=255)
                                                                                                                      cAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCAc                             <  1:1167720/150‑1 (MQ=255)
                                                                                                                         tAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGc                          >  2:1183640/1‑150 (MQ=255)
                                                                                                                         tAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGc                          >  1:1495238/1‑150 (MQ=255)
                                                                                                                            ccATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGATTTCGGGTTGACATGTCTTACAAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGc                       <  1:623185/150‑1 (MQ=255)
                                                                                                                            ccATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGc                       <  2:1388539/150‑1 (MQ=255)
                                                                                                                             cATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCg                      <  2:143483/150‑1 (MQ=255)
                                                                                                                              aTCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCgg                     <  2:1810697/150‑1 (MQ=255)
                                                                                                                               tCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGt                    <  1:875611/150‑1 (MQ=255)
                                                                                                                                   ttACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTgg                >  2:1564783/1‑150 (MQ=255)
                                                                                                                                    tggttgttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgt               >  2:1157311/7‑150 (MQ=255)
                                                                                                                                    tggttgttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgt               >  2:1495469/7‑150 (MQ=255)
                                                                                                                                    tACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCTGAATTCTGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgt               <  2:842511/150‑1 (MQ=255)
                                                                                                                                    tACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgt               <  2:948803/150‑1 (MQ=255)
                                                                                                                                    tACACTTTGCTGCACTTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAAACAATTTCAGAATTCGTGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgt               <  2:835265/150‑1 (MQ=255)
                                                                                                                                     aCCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAAATCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgtt              >  2:1229863/1‑150 (MQ=255)
                                                                                                                                      cccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttg             <  1:753789/150‑1 (MQ=255)
                                                                                                                                        cTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgtt           <  2:1612982/150‑1 (MQ=255)
                                                                                                                                            gctgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGTTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATc       >  1:305799/1‑150 (MQ=255)
                                                                                                                                              tgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCg     <  2:1004381/150‑1 (MQ=255)
                                                                                                                                               gcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCgg    <  2:32849/150‑1 (MQ=255)
                                                                                                                                                cAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGa   <  2:901419/150‑1 (MQ=255)
                                                                                                                                                cAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTATTACGCCATCTCATCCGATGCAACGCCACGGATGCGGTCTGGTTGTGCATCAGGa   >  1:1824892/1‑150 (MQ=255)
                                                                                                                                                 aaTTTGCAGCAACATCCTTGAGAGTGTAATTCTTGTTATGTTTTTATCCAATTTCAGAATTCGGGTTGACATGTCTTTCCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGAt  <  2:1081594/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGAGAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGAT  >  NC_000913/4551275‑4551569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: