Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,593,430 G→T 5.2% V431V (GTC→GTA) ‡ lgoT ← putative L‑galactonate:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,593,4300GT 5.2% 339.6 / 4.2 113V431V (GTC→GTA) ‡lgoTputative L‑galactonate:H+ symporter
Reads supporting (aligned to +/- strand):  ref base G (45/61);  new base T (2/5);  total (47/66)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

CAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGAC  >  NC_000913/4593281‑4593579
                                                                                                                                                     |                                                                                                                                                     
cAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTg                                                                                                                                                       <  1:1925569/150‑1 (MQ=255)
             taaCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAga                                                                                                                                          <  2:1692433/149‑1 (MQ=255)
              aaCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTTGCGTCACCGCAgcg                                                                                                                                         <  1:1368096/150‑3 (MQ=255)
              aaCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAgat                                                                                                                                         <  2:803914/150‑1 (MQ=255)
              aaCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGAGCCCCCGCTGCGGTTGCGTCACCGCAgcg                                                                                                                                         <  1:1183881/150‑3 (MQ=255)
                 tGGGAAACATTTTAATCGCCATGAAAATGCCCGATCAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAgatgat                                                                                                                                      <  2:1249555/150‑1 (MQ=255)
                 tGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTTGCGTCACCGCAgcgggg                                                                                                                                      <  2:851726/150‑6 (MQ=255)
                 cgggAAACTTTTTAATCGCCCTGAATCTGCCCGATAACCAAAATGTTATCGGGCATAAGGTGACTAATCTTTACGTGGGTCGTTGATGGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAgatgat                                                                                                                                      <  2:1243238/149‑1 (MQ=255)
                  gggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTTTCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACTCCTGTGCCCCCGCTGCGGTGACGCAACCGCTGATGAtt                                                                                                                                     >  1:360500/1‑150 (MQ=255)
                  gggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGAtt                                                                                                                                     <  2:389897/150‑1 (MQ=255)
                  gggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGAtt                                                                                                                                     <  2:976877/150‑1 (MQ=255)
                  gggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGATGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGAtt                                                                                                                                     >  2:1887165/1‑150 (MQ=255)
                       aCATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGc                                                                                                                                <  2:1429676/150‑1 (MQ=255)
                        cattgaaaTCGCCATGAAAATGCCCGATAAGCAAAATGTAAGCGCGCATAAGGAGATTAATCTTTACGTGGGTCGTTGCTCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGcc                                                                                                                               <  1:372030/144‑1 (MQ=255)
                         aTTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCa                                                                                                                              >  2:1172891/1‑150 (MQ=255)
                         aTTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCa                                                                                                                              >  2:1160286/1‑150 (MQ=255)
                          ttttAATCGCCATGAAAATGCCCGATATGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAg                                                                                                                             >  1:1789783/1‑150 (MQ=255)
                              aaTCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACgg                                                                                                                         <  2:399900/150‑1 (MQ=255)
                                  gCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATg                                                                                                                     <  1:475367/150‑1 (MQ=255)
                                   ccATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGCCGCAACCGCAGATGATTAGTGCCAGACGGAATGa                                                                                                                    >  1:682951/1‑150 (MQ=255)
                                      tGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCTGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCACCCGCAGATGATTAGTGCCAGACCGAATTAgtg                                                                                                                 >  2:371914/1‑150 (MQ=255)
                                      tGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAgtg                                                                                                                 >  1:1329848/1‑150 (MQ=255)
                                         aactGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCATTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAgtgggt                                                                                                              <  1:987640/147‑1 (MQ=255)
                                              cccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACTAGGAAGATGTACGCCAGTGCCCACGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTAt                                                                                                         >  1:1591429/1‑150 (MQ=255)
                                               ccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATc                                                                                                        <  1:29186/150‑1 (MQ=255)
                                                cGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATca                                                                                                       <  2:1888577/150‑1 (MQ=255)
                                                 caTAAGCAAAATGTTATCGGGAATAAGGAGCTTAATCTTTACTTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGGCCCCGCTGCGGGGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaa                                                                                                      <  2:1786893/149‑1 (MQ=255)
                                                  aTAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGGTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaac                                                                                                     <  2:559656/150‑1 (MQ=255)
                                                   tAAGCAAACTGTTATCGGGCATAAGGAGATTAATCTTAACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaaca                                                                                                    <  1:116576/150‑1 (MQ=255)
                                                    acgCAAAATGTTATCGGGCATAAGGAGTTTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCTCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaacaa                                                                                                   <  1:1072306/148‑1 (MQ=255)
                                                     aGCAAAATTTAATCGGGAATAAGGAGAATAATCTTAACGTGGGTCGTTGAACGACTGACGAACCAGGAAGATGTACGCCAGAGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAAt                                                                                                  <  1:1760222/150‑1 (MQ=255)
                                                     aGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAAt                                                                                                  <  2:23564/150‑1 (MQ=255)
                                                      gCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATa                                                                                                 >  2:1166480/1‑150 (MQ=255)
                                                      gCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATa                                                                                                 >  2:1841253/1‑150 (MQ=255)
                                                      gCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATa                                                                                                 >  1:673362/1‑150 (MQ=255)
                                                      gCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATa                                                                                                 >  1:840993/1‑150 (MQ=255)
                                                           aTGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACTAAACCGCAGATGATTAGTGCCAGACGGAATGAGTCGGTTTTGTCAACAAAAAAAcc                                                                                            >  1:946638/1‑150 (MQ=255)
                                                           aTGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAAcc                                                                                            <  2:938159/150‑1 (MQ=255)
                                                              ttATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGt                                                                                         >  2:1869123/1‑150 (MQ=255)
                                                               tATCGGGCATAAGGAGATTAATCTTTACGTTGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCGGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTa                                                                                        <  1:987092/150‑1 (MQ=255)
                                                                aTCGGGCATAAGGAGATAAATCTTTACGTGGGTCGTTGATCGGCTGACGAATCAGGAAGATGTACGCCAGTGCCCCCTCTGCGGTGACGCAAACGCAGATGATTAGTGACAGACGCAATGAGTGGGTGGTATCAACAATAAAACCAGTaa                                                                                       <  1:355226/150‑1 (MQ=255)
                                                                  cGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATg                                                                                     <  2:1571565/150‑1 (MQ=255)
                                                                        tAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgc                                                                               <  2:129388/150‑1 (MQ=255)
                                                                         aaGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcg                                                                              >  2:820008/1‑150 (MQ=255)
                                                                         aaGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcg                                                                              <  1:1121091/150‑1 (MQ=255)
                                                                         aaGGAGATTAATCTTTACGTGGGTCGGTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcg                                                                              <  1:371304/150‑1 (MQ=255)
                                                                          aGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcgc                                                                             >  2:1498903/1‑150 (MQ=255)
                                                                          aGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCACCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTCGTATCAACAATAAAACCAGTAATGATCGtcgc                                                                             >  1:1632625/1‑150 (MQ=255)
                                                                           ggAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCa                                                                            >  1:1819484/1‑150 (MQ=255)
                                                                            tagaTTAAGCTTTACGTGGGTCGTTGATCGGCATACGAACCAGGAAGATTTACGCCAGTGCCCCCGCTGTGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGAAGCAACAATAAAACCAGAACTGATCGGCGCaa                                                                           <  1:311706/149‑1 (MQ=255)
                                                                              gaTTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCCACCGCAGATGATTAGTGCCAGCCGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGAaaaa                                                                         >  2:72565/1‑150 (MQ=255)
                                                                              gaTTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAACCCAGTAATGATCGGCGCaaaa                                                                         >  2:759822/1‑150 (MQ=255)
                                                                              gaTTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCaaaa                                                                         <  2:513208/150‑1 (MQ=255)
                                                                               aTTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAag                                                                        <  2:1299984/150‑1 (MQ=255)
                                                                                  aaTCTTTACGTGGGTCGTTGATCTGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGAGCGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAgg                                                                     >  1:1471473/1‑150 (MQ=255)
                                                                                  aaTCTTAACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAgg                                                                     <  2:994397/150‑1 (MQ=255)
                                                                                     cTTTACGTGGGACGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAAACGCAGAAGATTAGAGCCAGCCGGAATGACTGGGTGGTATCCACAATACACCCCGTAATGATCGGCGCAAAAGCGgcgc                                                                  >  2:1083742/1‑150 (MQ=255)
                                                                                       ttACGTGGGTCGTGGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAg                                                                <  2:336787/150‑1 (MQ=255)
                                                                                            tGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGaa                                                           <  2:32858/150‑1 (MQ=255)
                                                                                              gttCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGc                                                         <  1:246414/148‑1 (MQ=255)
                                                                                               gTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCACCCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCt                                                        >  1:1301628/1‑150 (MQ=255)
                                                                                                gcGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTg                                                       <  2:1709274/149‑1 (MQ=255)
                                                                                                 cGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTgg                                                      <  2:1426657/150‑1 (MQ=255)
                                                                                                  gTTGATCGGCTGACGTACCAGGATGATGGACCCCAGGGCCCCGGCTGCGGGTACGCAACCGAATATGAGTTGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAATAGAGGCGCAGATGAAGCTGGc                                                     <  1:490583/150‑1 (MQ=255)
                                                                                                    tgctCGGCTGACGAACCAGGAAGATGTACGCCAGAGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCaa                                                   <  1:1799410/147‑1 (MQ=255)
                                                                                                      aTCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAg                                                 >  2:209905/1‑150 (MQ=255)
                                                                                                           cTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTg                                            <  2:1110471/150‑1 (MQ=255)
                                                                                                           cTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGGGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTg                                            <  2:1167768/150‑1 (MQ=255)
                                                                                                            tGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTgg                                           <  2:197281/150‑1 (MQ=255)
                                                                                                             gACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGa                                          <  1:1417270/150‑1 (MQ=255)
                                                                                                              aCGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGAt                                         <  1:1163501/150‑1 (MQ=255)
                                                                                                               cGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATa                                        >  2:1825562/1‑150 (MQ=255)
                                                                                                                   ccAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGc                                    <  2:1718633/150‑1 (MQ=255)
                                                                                                                    cAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGcc                                   >  2:1476299/1‑150 (MQ=255)
                                                                                                                      ggAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCa                                 <  1:1060386/150‑1 (MQ=255)
                                                                                                                           aTGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCACCCCGAc                            >  2:1840806/1‑150 (MQ=255)
                                                                                                                            tGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACg                           >  2:1667153/1‑150 (MQ=255)
                                                                                                                            tGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACg                           <  1:18308/150‑1 (MQ=255)
                                                                                                                             gTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGc                          <  1:1732027/150‑1 (MQ=255)
                                                                                                                             gTACGCCAGTCCCCCCGCGGCGGGGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGTTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGc                          <  2:360500/150‑1 (MQ=255)
                                                                                                                               aCGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAg                        <  2:1840281/150‑1 (MQ=255)
                                                                                                                                cGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGGAGt                       >  2:814223/1‑150 (MQ=255)
                                                                                                                                cGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGCAGt                       >  1:729986/1‑150 (MQ=255)
                                                                                                                                cGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGt                       >  1:686876/1‑150 (MQ=255)
                                                                                                                                     gTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGc                  <  2:218923/150‑1 (MQ=255)
                                                                                                                                      tGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCg                 <  1:833194/150‑1 (MQ=255)
                                                                                                                                        cccccGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCgag               <  1:424182/150‑1 (MQ=255)
                                                                                                                                         ccgcgcgGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTAGCAACAATAAACCCAGTAATGCTCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCgaga              <  1:1735698/147‑1 (MQ=255)
                                                                                                                                         ccccGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCgaga              >  2:111626/1‑150 (MQ=255)
                                                                                                                                         ccccGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCgaga              >  1:1587386/1‑150 (MQ=255)
                                                                                                                                         ccccGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGATCTGGATACTGCCCACCGACGCAGTCATGCgaga              >  1:196296/1‑150 (MQ=255)
                                                                                                                                         ccccGCGGCGGTGACGCAACCGCAGAAGATTAGTGCCAGTCGGAATGAGTGTGTGGTATCAACAAAAATACCAGTAAAGATCGGCGCAAACGAGGCGCATATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCgaga              <  1:1727573/150‑1 (MQ=255)
                                                                                                                                          cccGCTGAGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGaa             >  2:909502/1‑150 (MQ=255)
                                                                                                                                            atcTGCGGTTGCGTCACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGc           >  1:1080628/3‑150 (MQ=255)
                                                                                                                                             tcTGCGGTTGCGTCACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGCAGTCATGCGAGAAGcc          >  1:1938920/2‑149 (MQ=255)
                                                                                                                                             gCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCa          >  2:208717/1‑150 (MQ=255)
                                                                                                                                              cTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCaa         >  1:1047029/1‑150 (MQ=255)
                                                                                                                                               tGCGGTGACGCAACCGCAGATGATTAGTGCCAGACTGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAAc        <  1:174684/150‑1 (MQ=255)
                                                                                                                                                 gggTGACGCAACCGCAGATGATTAGTGCCATACGGAATGTGTGGGTGGTATCAACAATAAAACGACTATTGATCGGCGCACAAGAGTCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCTTGCGTGAAGCAACTg      <  2:11449/149‑1 (MQ=255)
                                                                                                                                                   gTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCg    >  2:1550019/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGAC  >  NC_000913/4593281‑4593579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: