Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,593,435 A→C 5.5% C430G (TGC→GGC)  lgoT ← putative L‑galactonate:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,593,4350AC 5.5% 368.9 / 7.6 120C430G (TGC→GGC) lgoTputative L‑galactonate:H+ symporter
Reads supporting (aligned to +/- strand):  ref base A (48/65);  new base C (3/4);  total (51/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGA  >  NC_000913/4593294‑4593584
                                                                                                                                             |                                                                                                                                                     
taaCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAga                                                                                                                                               <  2:1692433/149‑1 (MQ=255)
 aaCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTTGCGTCACCGCAgcg                                                                                                                                              <  1:1368096/150‑3 (MQ=255)
 aaCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAgat                                                                                                                                              <  2:803914/150‑1 (MQ=255)
 aaCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGAGCCCCCGCTGCGGTTGCGTCACCGCAgcg                                                                                                                                              <  1:1183881/150‑3 (MQ=255)
    tGGGAAACATTTTAATCGCCATGAAAATGCCCGATCAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAgatgat                                                                                                                                           <  2:1249555/150‑1 (MQ=255)
    cgggAAACTTTTTAATCGCCCTGAATCTGCCCGATAACCAAAATGTTATCGGGCATAAGGTGACTAATCTTTACGTGGGTCGTTGATGGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAgatgat                                                                                                                                           <  2:1243238/149‑1 (MQ=255)
     gggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTTTCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACTCCTGTGCCCCCGCTGCGGTGACGCAACCGCTGATGAtt                                                                                                                                          >  1:360500/1‑150 (MQ=255)
     gggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGAtt                                                                                                                                          <  2:976877/150‑1 (MQ=255)
     gggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGAtt                                                                                                                                          <  2:389897/150‑1 (MQ=255)
     gggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGATGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGAtt                                                                                                                                          >  2:1887165/1‑150 (MQ=255)
         aaCATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTg                                                                                                                                      <  2:1530280/150‑1 (MQ=255)
          aCATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGc                                                                                                                                     <  2:1429676/150‑1 (MQ=255)
            aTTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCa                                                                                                                                   >  2:1172891/1‑150 (MQ=255)
            aTTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCa                                                                                                                                   >  2:1160286/1‑150 (MQ=255)
             ttttAATCGCCATGAAAATGCCCGATATGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAg                                                                                                                                  >  1:1789783/1‑150 (MQ=255)
             ttttAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAg                                                                                                                                  <  2:1727663/150‑1 (MQ=255)
                 aaTCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACgg                                                                                                                              <  2:399900/150‑1 (MQ=255)
                    cGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGGGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAAt                                                                                                                           <  1:1475395/150‑1 (MQ=255)
                     gCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATg                                                                                                                          <  1:475367/150‑1 (MQ=255)
                            aactGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCATTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAgtgggt                                                                                                                   <  1:987640/147‑1 (MQ=255)
                                 cccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACTAGGAAGATGTACGCCAGTGCCCACGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTAt                                                                                                              >  1:1591429/1‑150 (MQ=255)
                                   cGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATca                                                                                                            <  2:1888577/150‑1 (MQ=255)
                                    caTAAGCAAAATGTTATCGGGAATAAGGAGCTTAATCTTTACTTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGGCCCCGCTGCGGGGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaa                                                                                                           <  2:1786893/149‑1 (MQ=255)
                                     aTAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGGTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaac                                                                                                          <  2:559656/150‑1 (MQ=255)
                                      tAAGCAAACTGTTATCGGGCATAAGGAGATTAATCTTAACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaaca                                                                                                         <  1:116576/150‑1 (MQ=255)
                                       acgCAAAATGTTATCGGGCATAAGGAGTTTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCTCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaacaa                                                                                                        <  1:1072306/148‑1 (MQ=255)
                                        aGCAAAATTTAATCGGGAATAAGGAGAATAATCTTAACGTGGGTCGTTGAACGACTGACGAACCAGGAAGATGTACGCCAGAGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAAt                                                                                                       <  1:1760222/150‑1 (MQ=255)
                                         gCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATa                                                                                                      >  1:624745/1‑150 (MQ=255)
                                         gCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATa                                                                                                      >  1:840993/1‑150 (MQ=255)
                                         gCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATa                                                                                                      >  1:673362/1‑150 (MQ=255)
                                         gCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATa                                                                                                      >  2:1841253/1‑150 (MQ=255)
                                              aTGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACTAAACCGCAGATGATTAGTGCCAGACGGAATGAGTCGGTTTTGTCAACAAAAAAAcc                                                                                                 >  1:946638/1‑150 (MQ=255)
                                              aTGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAAcc                                                                                                 <  2:938159/150‑1 (MQ=255)
                                               agTTATGCGGCATAAGGAGATTAATCATTACCTGCGTCGTTCATCGGCCGACGACCCAGGAAGATGTACGCCATTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCa                                                                                                <  2:114059/149‑1 (MQ=255)
                                                 ttATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGt                                                                                              >  2:1869123/1‑150 (MQ=255)
                                                  tATCGGGCATAAGGAGATTAATCTTTACGTTGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCGGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTa                                                                                             <  1:987092/150‑1 (MQ=255)
                                                   aTCGGGCATAAGGAGATAAATCTTTACGTGGGTCGTTGATCGGCTGACGAATCAGGAAGATGTACGCCAGTGCCCCCTCTGCGGTGACGCAAACGCAGATGATTAGTGACAGACGCAATGAGTGGGTGGTATCAACAATAAAACCAGTaa                                                                                            <  1:355226/150‑1 (MQ=255)
                                                     cGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATg                                                                                          <  2:1571565/150‑1 (MQ=255)
                                                       ggCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGAt                                                                                        >  2:1882388/1‑150 (MQ=255)
                                                            aaGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcg                                                                                   >  2:820008/1‑150 (MQ=255)
                                                            aaGGAGATTAATCTTTACGTGGGTCGGTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcg                                                                                   <  1:371304/150‑1 (MQ=255)
                                                            aaGGAGATTAAGCTTTACGTGGGTCGTGGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcg                                                                                   <  2:1251631/150‑1 (MQ=255)
                                                             aGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCACCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTCGTATCAACAATAAAACCAGTAATGATCGtcgc                                                                                  >  1:1632625/1‑150 (MQ=255)
                                                               tagaTTAAGCTTTACGTGGGTCGTTGATCGGCATACGAACCAGGAAGATTTACGCCAGTGCCCCCGCTGTGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGAAGCAACAATAAAACCAGAACTGATCGGCGCaa                                                                                <  1:311706/149‑1 (MQ=255)
                                                                 gaTTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAACCCAGTAATGATCGGCGCaaaa                                                                              >  2:759822/1‑150 (MQ=255)
                                                                 gaTTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCaaaa                                                                              <  2:513208/150‑1 (MQ=255)
                                                                  aTTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAag                                                                             <  2:1299984/150‑1 (MQ=255)
                                                                     aaTCTTTACGTGGGTCGTTGATCTGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGAGCGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAgg                                                                          >  1:1471473/1‑150 (MQ=255)
                                                                     aaTCTTAACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAgg                                                                          <  2:994397/150‑1 (MQ=255)
                                                                         tttACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCa                                                                      <  2:818529/150‑1 (MQ=255)
                                                                          ttACGTGGGTCGTGGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAg                                                                     <  2:336787/150‑1 (MQ=255)
                                                                             cGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATg                                                                  >  2:424182/1‑150 (MQ=255)
                                                                               tGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGaa                                                                <  2:32858/150‑1 (MQ=255)
                                                                                 gttCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGc                                                              <  1:246414/148‑1 (MQ=255)
                                                                                  gTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCACCCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCt                                                             >  1:1301628/1‑150 (MQ=255)
                                                                                    cGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTgg                                                           <  2:1426657/150‑1 (MQ=255)
                                                                                     gTTGATCGGCTGACGTACCAGGATGATGGACCCCAGGGCCCCGGCTGCGGGTACGCAACCGAATATGAGTTGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAATAGAGGCGCAGATGAAGCTGGc                                                          <  1:490583/150‑1 (MQ=255)
                                                                                       tgctCGGCTGACGAACCAGGAAGATGTACGCCAGAGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCaa                                                        <  1:1799410/147‑1 (MQ=255)
                                                                                         aTCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAg                                                      >  2:209905/1‑150 (MQ=255)
                                                                                              cTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTg                                                 <  2:1110471/150‑1 (MQ=255)
                                                                                              cTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGGGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTg                                                 <  2:1167768/150‑1 (MQ=255)
                                                                                                gACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGa                                               <  1:1417270/150‑1 (MQ=255)
                                                                                                 aCGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGAt                                              <  1:1163501/150‑1 (MQ=255)
                                                                                                  cGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATa                                             >  2:1825562/1‑150 (MQ=255)
                                                                                                      ccAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGc                                         <  2:1718633/150‑1 (MQ=255)
                                                                                                       cAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGcc                                        >  2:1476299/1‑150 (MQ=255)
                                                                                                        aGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGccc                                       <  1:1166480/150‑1 (MQ=255)
                                                                                                         ggAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCa                                      <  1:1060386/150‑1 (MQ=255)
                                                                                                               tGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACg                                >  2:1667153/1‑150 (MQ=255)
                                                                                                               tGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACg                                <  1:18308/150‑1 (MQ=255)
                                                                                                                gTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGc                               <  1:1732027/150‑1 (MQ=255)
                                                                                                                gTACGCCAGTCCCCCCGCGGCGGGGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGTTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGc                               <  2:360500/150‑1 (MQ=255)
                                                                                                                  aCGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAg                             <  2:1840281/150‑1 (MQ=255)
                                                                                                                   cGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGGAGt                            >  2:814223/1‑150 (MQ=255)
                                                                                                                   cGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGCAGt                            >  1:729986/1‑150 (MQ=255)
                                                                                                                   cGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGt                            >  1:686876/1‑150 (MQ=255)
                                                                                                                   cGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGt                            >  1:1846373/1‑150 (MQ=255)
                                                                                                                        gTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGc                       <  2:218923/150‑1 (MQ=255)
                                                                                                                         tGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCg                      <  1:833194/150‑1 (MQ=255)
                                                                                                                           cccccGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCgag                    <  1:424182/150‑1 (MQ=255)
                                                                                                                            ccgcgcgGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTAGCAACAATAAACCCAGTAATGCTCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCgaga                   <  1:1735698/147‑1 (MQ=255)
                                                                                                                            ccccGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCTGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGCAGTCATGCgaga                   >  1:358850/1‑150 (MQ=255)
                                                                                                                            ccccGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCgaga                   >  2:111626/1‑150 (MQ=255)
                                                                                                                            ccccGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGATCTGGATACTGCCCACCGACGCAGTCATGCgaga                   >  1:196296/1‑150 (MQ=255)
                                                                                                                            ccccGCGGCGGTGACGCAACCGCAGAAGATTAGTGCCAGTCGGAATGAGTGTGTGGTATCAACAAAAATACCAGTAAAGATCGGCGCAAACGAGGCGCATATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCgaga                   <  1:1727573/150‑1 (MQ=255)
                                                                                                                             cccGCTGAGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGaa                  >  2:909502/1‑150 (MQ=255)
                                                                                                                               atcTGCGGTTGCGTCACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGc                >  1:1080628/3‑150 (MQ=255)
                                                                                                                                tcTGCGGTTGCGTCACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGCAGTCATGCGAGAAGcc               >  1:1938920/2‑149 (MQ=255)
                                                                                                                                gCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCa               >  2:208717/1‑150 (MQ=255)
                                                                                                                                 cTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCaa              >  1:1047029/1‑150 (MQ=255)
                                                                                                                                  tGCGGTGACGCAACCGCAGATGATTAGTGCCAGACTGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAAc             <  1:174684/150‑1 (MQ=255)
                                                                                                                                    gggTGACGCAACCGCAGATGATTAGTGCCATACGGAATGTGTGGGTGGTATCAACAATAAAACGACTATTGATCGGCGCACAAGAGTCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCTTGCGTGAAGCAACTg           <  2:11449/149‑1 (MQ=255)
                                                                                                                                      gTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCg         >  2:1550019/1‑150 (MQ=255)
                                                                                                                                       ggACGCATCCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGa        <  1:894457/149‑1 (MQ=255)
                                                                                                                                        gACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGAc       <  2:248792/150‑1 (MQ=255)
                                                                                                                                         aCGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACg      >  1:1480684/1‑150 (MQ=255)
                                                                                                                                          cGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGt     >  2:1991552/1‑150 (MQ=255)
                                                                                                                                          cGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGt     >  1:908337/1‑150 (MQ=255)
                                                                                                                                            cAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTgg   >  1:1107809/1‑150 (MQ=255)
                                                                                                                                             aaCCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGa  >  2:250879/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
GAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGTGGA  >  NC_000913/4593294‑4593584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: