Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,618,053 T→G 5.5% L244R (CTG→CGG)  deoC → 2‑deoxyribose‑5‑phosphate aldolase, NAD(P)‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,618,0530TG 5.5% 386.1 / 8.3 130L244R (CTG→CGG) deoC2‑deoxyribose‑5‑phosphate aldolase, NAD(P)‑linked
Reads supporting (aligned to +/- strand):  ref base T (73/49);  new base G (3/5);  total (76/54)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 140 total aligned reads displayed.

GAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCC  >  NC_000913/4617905‑4618201
                                                                                                                                                    |                                                                                                                                                    
gAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCctg                                                                                                                                                     >  1:249001/1‑150 (MQ=255)
gAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCctg                                                                                                                                                     <  1:294438/150‑1 (MQ=255)
gAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCctg                                                                                                                                                     <  2:873802/150‑1 (MQ=255)
 aaaaaaCCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCctgc                                                                                                                                                    >  2:1868175/1‑150 (MQ=255)
  aaaaaCCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCGATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCctgct                                                                                                                                                   >  1:16352/1‑150 (MQ=255)
  aaaaaCCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCctgct                                                                                                                                                   >  1:155131/1‑150 (MQ=255)
  aaaaaCCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCctgct                                                                                                                                                   >  1:403996/1‑150 (MQ=255)
   aaacCCGTTGGGTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCctgctg                                                                                                                                                  <  2:1507625/150‑1 (MQ=255)
        cGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAgc                                                                                                                                             <  1:896680/150‑1 (MQ=255)
         gTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAgcg                                                                                                                                            <  1:6400/150‑1 (MQ=255)
            ggTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTg                                                                                                                                         <  2:1165809/150‑1 (MQ=255)
               ttCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTgggg                                                                                                                                      >  2:1597750/1‑149 (MQ=255)
                 cAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCa                                                                                                                                    >  1:637553/1‑150 (MQ=255)
                 cAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCa                                                                                                                                    >  2:1563945/1‑150 (MQ=255)
                    aCCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACgg                                                                                                                                 <  2:728910/150‑1 (MQ=255)
                        gcgggcggTGTGCGTACTGCGGAAGATGAGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTTCTGACTGGGCAGATGCGCGGCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGAc                                                                                                                             <  2:1341951/150‑1 (MQ=255)
                            gcggcgTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTa                                                                                                                         >  1:932824/1‑150 (MQ=255)
                                cgtgcgtACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGCCGGTAagag                                                                                                                     >  2:1285223/1‑150 (MQ=255)
                                            ggAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGCCTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAACAGCGCCAGCAGcta                                                                                                         >  2:1763813/1‑150 (MQ=255)
                                               aGATGCGCAGAAATATCTCGCCATTGCAGATTAACTGTTCGGCGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGctacta                                                                                                      <  1:989325/150‑1 (MQ=255)
                                               aGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGctacta                                                                                                      <  1:6037/150‑1 (MQ=255)
                                                 aTGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACtaag                                                                                                    >  1:594324/1‑150 (MQ=255)
                                                   gcgcAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCCGCTACtaagta                                                                                                  >  2:979761/1‑150 (MQ=255)
                                                   gcgcAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACtaagta                                                                                                  <  2:301270/150‑1 (MQ=255)
                                                   gcgcAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACtaagta                                                                                                  >  2:346473/1‑150 (MQ=255)
                                                    cgcAGAAATATCTCGCCTTTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTGCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCTACGGTAAGAGCGCCAGCAGCTACtaagtaa                                                                                                 <  2:1179943/150‑1 (MQ=255)
                                                    cgcAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACtaagtaa                                                                                                 >  2:2016309/1‑150 (MQ=255)
                                                     gcAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACtaagtaag                                                                                                <  2:811329/150‑1 (MQ=255)
                                                      cAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGa                                                                                               >  2:891710/1‑150 (MQ=255)
                                                      cAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGa                                                                                               <  2:1617244/150‑1 (MQ=255)
                                                           atatCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCGGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCtt                                                                                          <  2:1088592/150‑1 (MQ=255)
                                                              tctcGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGCTGCTTTAc                                                                                       >  1:658903/1‑150 (MQ=255)
                                                              tctcGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTAc                                                                                       >  1:1237494/1‑150 (MQ=255)
                                                              tctcGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTAc                                                                                       >  1:1407242/1‑150 (MQ=255)
                                                              tctcGCCATTGCAGATGAACTGTGCGGTGCTGACTTGGCAGATGCGCGTCACTACCGCTGTGGCGCTTCCAGCCTGCTGGCAAGCCGGCTGAAAGCGCTGGGGCACGGCGACGGGAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTAc                                                                                       <  1:317197/150‑1 (MQ=255)
                                                               ctcGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACg                                                                                      <  1:2000971/150‑1 (MQ=255)
                                                               ctcGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACg                                                                                      >  2:756505/1‑150 (MQ=255)
                                                                tcGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTGCTAAGTAAGCTGCTTTACGc                                                                                     >  2:1066274/1‑150 (MQ=255)
                                                                   ccATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTg                                                                                  <  2:1589346/150‑1 (MQ=255)
                                                                   ccATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCGGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTg                                                                                  <  2:1585905/150‑1 (MQ=255)
                                                                       tGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGAAGCTTTACGCCTGAtgc                                                                              >  2:1301780/1‑150 (MQ=255)
                                                                         cAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgc                                                                            >  1:1156925/1‑150 (MQ=255)
                                                                           gATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGCCGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgctg                                                                          >  1:802299/1‑150 (MQ=255)
                                                                           gATGAACTGTCCGGGGCTGCCTGGGCAGACGCGCGGCCCTACCGCTTGGGCGCTTCCAGCCTGCTGGCAAGCCTGCGGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgctg                                                                          <  2:64505/150‑1 (MQ=255)
                                                                            aTGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgctgc                                                                         <  1:1102427/150‑1 (MQ=255)
                                                                              gAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGAtgcgctgcgc                                                                       <  2:837869/150‑1 (MQ=255)
                                                                               aaCTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATgcgctgcgct                                                                      >  1:864834/1‑150 (MQ=255)
                                                                                     tCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGACAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAg                                                                >  1:668394/1‑150 (MQ=255)
                                                                                     tCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAg                                                                >  2:741422/1‑150 (MQ=255)
                                                                                      gggTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCACGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAgg                                                               >  1:457817/2‑150 (MQ=255)
                                                                                       ggTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGc                                                              >  2:1616826/1‑150 (MQ=255)
                                                                                        gTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGcc                                                             <  1:447182/150‑1 (MQ=255)
                                                                                        gTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGcc                                                             <  2:558433/150‑1 (MQ=255)
                                                                                         tGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCt                                                            >  1:1103954/1‑150 (MQ=255)
                                                                                          gCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTa                                                           >  1:210696/1‑150 (MQ=255)
                                                                                             gACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACga                                                        >  2:1985798/1‑150 (MQ=255)
                                                                                             gACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACga                                                        >  1:1473546/1‑150 (MQ=255)
                                                                                               cTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACgaga                                                      >  2:278619/1‑150 (MQ=255)
                                                                                               cTGCGCAGATGCGCGTCACTACCGCTTAGGAGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTAACGGCGACGGTAAGAGCGCCAGCATCTACTAAGTAAGATGCTTTACTCCTGATGCGATTCGCTTATCAGGCCTACgagc                                                      >  2:427179/1‑149 (MQ=255)
                                                                                                 gggCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACg                                                    <  1:1689223/150‑1 (MQ=255)
                                                                                                 gggCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACg                                                    >  2:505673/1‑150 (MQ=255)
                                                                                                 gggCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACg                                                    <  1:1653959/150‑1 (MQ=255)
                                                                                                   gCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTa                                                  <  2:430408/150‑1 (MQ=255)
                                                                                                        tGCGCGTCACTACCGCTTTGGCGCTTCACTCCTGCGGGCATGCCGGCTGAAAGCGCTTGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATTCTTTACGCCTTATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTAc                                             <  1:344803/150‑1 (MQ=255)
                                                                                                          cgcgTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCTCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTAccc                                           >  1:275548/1‑150 (MQ=255)
                                                                                                          cgcgTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTAccc                                           >  2:1840665/1‑150 (MQ=255)
                                                                                                          cgcgTCACTACCGCTTTGGCGCTTCCAGCCTACTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTAccc                                           <  2:1263008/150‑1 (MQ=255)
                                                                                                            cgTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCGGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGt                                         <  1:403324/150‑1 (MQ=255)
                                                                                                             gTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTa                                        <  2:855873/150‑1 (MQ=255)
                                                                                                             gTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTa                                        >  2:2014916/1‑150 (MQ=255)
                                                                                                              acACTACCGCTTTGTCGCTTCCATCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGTCGACGGTATGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAg                                       <  2:310993/149‑1 (MQ=255)
                                                                                                                aCTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCCGGCCTACGAGACGTATCTACCCGTAGGc                                     >  1:1549708/1‑150 (MQ=255)
                                                                                                                aCTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGc                                     >  1:1563041/1‑150 (MQ=255)
                                                                                                                 cTACCGCTTTGGCGCTTCCCCCCTGCAGGCAAGCCTGCAGCAAGCGCTGGGTCACCGCGACGGTAAGACCGCCCGCAGCTACGAAGTAAGCTGCTTTACGCCTGCTGCGCTGCGCTTATCAGGCCTACGCTACGGATCAACCCGTAGGcc                                    >  2:1761362/1‑150 (MQ=255)
                                                                                                                 cTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGcc                                    >  2:324267/1‑150 (MQ=255)
                                                                                                                   aCCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCgg                                  >  1:1071651/1‑150 (MQ=255)
                                                                                                                    ccGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGCCGTATCTACCCGTAGGCCGGa                                 >  1:77824/1‑150 (MQ=255)
                                                                                                                     tgacccagcgcTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGAt                                >  2:1470042/8‑150 (MQ=255)
                                                                                                                     cGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGAt                                >  1:137392/1‑150 (MQ=255)
                                                                                                                     cGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGAt                                <  2:1168677/150‑1 (MQ=255)
                                                                                                                       cTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTTAGAGCGCCAGCAGCTTCTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGCCGTATCTTCCCGTAGGCCGGCTaa                              >  1:287590/1‑150 (MQ=255)
                                                                                                                         ttGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAgg                            <  1:733914/150‑1 (MQ=255)
                                                                                                                         ccagcgcTTTCAGCAGGCTTGCCAGCAGGCTGGAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAgg                            >  2:966834/4‑150 (MQ=38)
                                                                                                                          tGGCGCTTCCATCCTGCTGGCAATCCTGCTGAAATCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAACATGCTCTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGc                           <  1:274175/150‑1 (MQ=255)
                                                                                                                          tGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGc                           >  1:1457875/1‑150 (MQ=255)
                                                                                                                           ggCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGGGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCg                          <  1:973016/150‑1 (MQ=255)
                                                                                                                           agcgcTTTCAGCAGGCTTGCCAGCAGGCTGGAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCg                          >  1:1413349/2‑150 (MQ=38)
                                                                                                                             cgcTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGCCGTATCTACCCGTAGGCCGGATAAGGCGTa                        >  1:288612/1‑150 (MQ=255)
                                                                                                                             cgcTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTa                        >  2:1957743/1‑150 (MQ=255)
                                                                                                                              gcTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAg                       <  2:471476/150‑1 (MQ=255)
                                                                                                                                 tCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACg                    <  2:70359/150‑1 (MQ=255)
                                                                                                                                 tCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACg                    >  1:181824/1‑150 (MQ=255)
                                                                                                                                     gCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATc                <  2:1407242/150‑1 (MQ=255)
                                                                                                                                        tgctgGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCgg             >  1:1889272/1‑150 (MQ=255)
                                                                                                                                         gctgGCAAGCCTGCTGAAAGCGCTGGGGCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGc            <  1:1743373/150‑1 (MQ=255)
                                                                                                                                             gCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCaaaa        >  2:1041651/1‑150 (MQ=255)
                                                                                                                                              cAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTTAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAg       >  1:522514/1‑150 (MQ=255)
                                                                                                                                              cAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCaaaac       >  2:1201805/1‑149 (MQ=255)
                                                                                                                                               aaGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAgc      <  1:2016309/150‑1 (MQ=255)
                                                                                                                                                aGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAgcc     <  1:406345/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTCACTACCGCTTTGGCGCTTCCAGCCTGCTGGCAAGCCTGCTGAAAGCGCTGGGTCACGGCGACGGTAAGAGCGCCAGCAGCTACTAAGTAAGATGCTTTACGCCTGATGCGCTGCGCTTATCAGGCCTACGAGACGTATCTACCCGTAGGCCGGATAAGGCGTAGACGCATCCGGCAAAAGCCGCC  >  NC_000913/4617905‑4618201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑