Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,624,359 A→G 5.0% L144P (CTG→CCG)  ytjB ← SMP_2 family putative membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,624,3590AG 5.0% 364.1 / 5.3 118L144P (CTG→CCG) ytjBSMP_2 family putative membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base A (59/53);  new base G (3/3);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 121 total aligned reads displayed.

ATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCG  >  NC_000913/4624210‑4624508
                                                                                                                                                     |                                                                                                                                                     
aTTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGTTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCa                                                                                                                                                       >  2:105984/1‑150 (MQ=255)
    ctgcCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGgcg                                                                                                                                                   >  1:1491470/1‑150 (MQ=255)
      gcCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCa                                                                                                                                                 >  2:2013027/1‑150 (MQ=255)
        cAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGGGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGa                                                                                                                                               <  1:954234/150‑1 (MQ=255)
         aGCGGGTGCGTTTCCCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTAGCCACCTGTTGGGCTTCGGGGGCGAGCGTATGGGTGGCGAGTGTCAGGCGCAGAt                                                                                                                                              <  2:1400350/150‑1 (MQ=255)
          gCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATa                                                                                                                                             >  2:1366781/1‑150 (MQ=255)
              gTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCg                                                                                                                                         <  2:670651/150‑1 (MQ=255)
                gCGTTTACCCTGTCGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGGATGGGTGGCGAGTGTCAGGCGCAGATAGCCGAg                                                                                                                                       <  2:308114/150‑1 (MQ=255)
                       cccTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCg                                                                                                                                >  1:938721/1‑150 (MQ=255)
                       cccTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCg                                                                                                                                >  2:435973/1‑150 (MQ=255)
                              gcagcGTGCGGGTCAGCACTACACCGAATGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGGGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGGCAGGCGCAGATAGCCGAGCGGTCCGTTTTTAc                                                                                                                         <  2:255639/150‑1 (MQ=255)
                                agcGTGCGGGGCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTAccc                                                                                                                       <  2:1905163/150‑1 (MQ=255)
                                 gcGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCg                                                                                                                      <  1:601134/150‑1 (MQ=255)
                                          gcAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAATTGGTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCt                                                                                                             <  2:1048546/149‑1 (MQ=255)
                                           cAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTc                                                                                                            >  2:598436/1‑150 (MQ=255)
                                           cAGCACTACACCGATTGCCAGGGAGAGCAGCAACATCAGGCGGAATATGTTAGTGGTGTTATCCACCTGTTGGGCTGCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTc                                                                                                            <  1:1210572/150‑1 (MQ=255)
                                            aGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCg                                                                                                           >  2:822398/1‑150 (MQ=255)
                                             gCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCCCATGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCAGCATTTGGCTCGa                                                                                                          >  2:1333108/1‑150 (MQ=255)
                                             gCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGTCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGa                                                                                                          >  2:1718457/1‑150 (MQ=255)
                                             gCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGa                                                                                                          >  1:886279/1‑150 (MQ=255)
                                             gCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGAGAGCGTATGGGTGTCGAGTGTCAGGCGCTGCTAGCCGAGCGGTCCGTTTTTAGCCGCAATTGGCTCGa                                                                                                          >  2:295123/1‑150 (MQ=255)
                                                  acacCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATc                                                                                                     >  2:698713/1‑150 (MQ=255)
                                                  acacCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTAGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCAGAGCGGTCCGTTTTTACCCGCAATTGGCTCGAGAATc                                                                                                     >  2:94502/1‑150 (MQ=255)
                                                   cacCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATct                                                                                                    >  1:1333706/1‑150 (MQ=255)
                                                     ccGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctgc                                                                                                  >  1:1109223/1‑150 (MQ=255)
                                                      cgtttGCCACTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctgct                                                                                                 <  2:404547/147‑1 (MQ=255)
                                                      cGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctgct                                                                                                 <  1:1773710/150‑1 (MQ=255)
                                                      cGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctgct                                                                                                 <  2:1958770/150‑1 (MQ=255)
                                                      cGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctgct                                                                                                 <  1:241322/150‑1 (MQ=255)
                                                       gATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctgctg                                                                                                <  2:190804/150‑1 (MQ=255)
                                                       gATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctgctg                                                                                                >  2:1751386/1‑150 (MQ=255)
                                                        aatGCCAGTGAGAGTAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTTGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTgg                                                                                               <  1:1294468/148‑1 (MQ=255)
                                                         ttGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGGCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGt                                                                                              <  2:1381108/150‑1 (MQ=255)
                                                           gCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTa                                                                                            >  1:849792/1‑150 (MQ=255)
                                                           gCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTa                                                                                            >  1:463330/1‑150 (MQ=255)
                                                           gCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGATGGGCTTCGGTGGCCAGCGAATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGCTTa                                                                                            >  1:374418/1‑150 (MQ=255)
                                                            ccAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGGGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGTCTCGACAATCTGCTGGTTaa                                                                                           >  2:971683/1‑150 (MQ=255)
                                                             cactGACAGCAGCAACATCAGTCGTAAAATGTTAGAGGCGTTATCCACCTGGTGGGCTTGGGTGGCGAGCGTATGGGTGGCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCGCGACAATCTGCTGGTTaaa                                                                                          <  2:955072/147‑1 (MQ=255)
                                                                 gagaGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTGCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAg                                                                                      <  2:443378/150‑1 (MQ=255)
                                                                  tgagCAGCAACATCAGGCGTAAAATGTTAGTGGTGGTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAgc                                                                                     <  2:52696/149‑1 (MQ=255)
                                                                   gagCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAgcc                                                                                    >  2:1791978/1‑150 (MQ=255)
                                                                     gcagcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAgccgc                                                                                  >  1:1137150/1‑150 (MQ=255)
                                                                      cagcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGATGGGCTTCGGAGGCGAGCGTATGGGTGTCGAGTGTCGGGCGCAGATAGCCGAGCGGTACGTTTTTTCCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAgccgcc                                                                                 >  1:1415599/1‑150 (MQ=255)
                                                                        gcaACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGCCAATCTGCTGGTTAAAATAGCCGCCTg                                                                               >  1:73976/1‑150 (MQ=255)
                                                                             aTCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCtttt                                                                          >  2:1845562/1‑150 (MQ=255)
                                                                                aGGCGTAAACTGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTa                                                                       <  1:601185/150‑1 (MQ=255)
                                                                                aGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTa                                                                       <  2:795759/150‑1 (MQ=255)
                                                                                 ggCGTAAAATGTTCGTGGTGTTTTCCACCTGTTTGGCTTCGGGGGCGTGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTAc                                                                      <  1:105984/150‑1 (MQ=255)
                                                                                 ggCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTAc                                                                      <  2:1804703/150‑1 (MQ=255)
                                                                                 ggCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTAc                                                                      >  2:1415565/1‑150 (MQ=255)
                                                                                   cGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCg                                                                    >  1:687329/1‑150 (MQ=255)
                                                                                    gTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGt                                                                   >  1:651385/1‑150 (MQ=255)
                                                                                      aaaaTGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAATTAGCCGCCTGCTTTTTTACCGTCg                                                                 <  2:1590750/150‑1 (MQ=255)
                                                                                       aaaTGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGa                                                                >  2:102183/1‑150 (MQ=255)
                                                                                       aaaTGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGa                                                                >  1:1155519/1‑150 (MQ=255)
                                                                                       aaaTGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTAAGGGTGTAGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCCCGTGCTTTTTTACCGTCGa                                                                >  2:75025/1‑150 (MQ=255)
                                                                                          tGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAgcg                                                             >  1:1388500/1‑150 (MQ=255)
                                                                                          tGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAgcg                                                             <  1:1366781/150‑1 (MQ=255)
                                                                                            gtAGTGGGGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGcc                                                           <  2:1410160/149‑1 (MQ=255)
                                                                                             tAGTGGTGTTATCCACCTGTTTGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCa                                                          <  2:885354/150‑1 (MQ=255)
                                                                                             tAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCa                                                          >  1:1257342/1‑150 (MQ=255)
                                                                                             tAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTCTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCa                                                          <  2:1948685/150‑1 (MQ=255)
                                                                                               gtggtgTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGc                                                        >  2:1812512/1‑150 (MQ=255)
                                                                                               gtggtgTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCATTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGc                                                        >  1:1730447/1‑150 (MQ=255)
                                                                                                tggtgTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGcc                                                       >  2:1682635/1‑150 (MQ=255)
                                                                                                 ggtgTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGATCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTTCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCg                                                      >  2:164298/1‑150 (MQ=255)
                                                                                                     ttATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTcg                                                  >  1:1722318/1‑150 (MQ=255)
                                                                                                       aTCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGGCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTcgcg                                                <  1:442216/150‑1 (MQ=255)
                                                                                                       aTCCACCTGTTGGGCTGCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTcgcg                                                <  1:607614/150‑1 (MQ=255)
                                                                                                        tCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCgcgc                                               >  2:591954/1‑150 (MQ=255)
                                                                                                        tCCACCTGTTGGGCTTCGGGGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCgcgc                                               <  1:1010105/150‑1 (MQ=255)
                                                                                                         ccACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGCGCGGTCCGTTTTTACACTCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGATCGCCAGCAGGTCGCGCa                                              >  2:1365873/1‑150 (MQ=255)
                                                                                                          cACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCAc                                             <  1:39844/150‑1 (MQ=255)
                                                                                                          cACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCAc                                             >  1:1153335/1‑150 (MQ=255)
                                                                                                                     cTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCttt                                  >  1:215689/1‑150 (MQ=255)
                                                                                                                       tCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCg                                <  2:1508858/150‑1 (MQ=255)
                                                                                                                        cGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGc                               >  2:881613/1‑150 (MQ=255)
                                                                                                                         ggTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGcc                              <  2:573353/150‑1 (MQ=255)
                                                                                                                         ggTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAACCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGcc                              <  1:415615/150‑1 (MQ=255)
                                                                                                                          gTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCa                             >  2:1343968/1‑150 (MQ=255)
                                                                                                                           tGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAg                            >  1:232149/1‑150 (MQ=255)
                                                                                                                              cGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGCCGCTTTCGCCAGAAc                         >  2:1317244/1‑150 (MQ=255)
                                                                                                                                  cGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGc                     <  1:2013027/150‑1 (MQ=255)
                                                                                                                                  cGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGc                     <  2:1791616/150‑1 (MQ=255)
                                                                                                                                  cGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGc                     <  1:1536289/150‑1 (MQ=255)
                                                                                                                                   gTATGGGTGTCGAGTGTCGGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCg                    <  2:1367999/150‑1 (MQ=255)
                                                                                                                                   gTATGGGGTGCGAGTGGCGGGCGGAGATACCCGAGCGGGCCGTTTTTACCCGCAAATGGCTAGCCAATATGCTGGTTAAAATAGCCGCCTGCATTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCg                    <  2:1415599/150‑1 (MQ=255)
                                                                                                                                    tATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGa                   >  1:1329269/1‑150 (MQ=255)
                                                                                                                                      tGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATa                 >  1:1026137/1‑150 (MQ=255)
                                                                                                                                      tGGGTGTCGAGGGTCAGGCGCTGAAAGCCGAGCGGTCCGTTATTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATa                 <  2:913418/150‑1 (MQ=255)
                                                                                                                                        cgtgtCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAg               >  1:1217727/2‑150 (MQ=255)
                                                                                                                                         gtgtCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCATTTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGGCAGAACGTGCGATAAGa              >  1:1012206/1‑150 (MQ=255)
                                                                                                                                           gtCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGGTTTTACCCGCATTTGGCTCGACAATCTGCTGGTTAAAATATCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCGTTCGCCAGAACGTGCGAAAAGATc            >  1:1956672/1‑150 (MQ=255)
                                                                                                                                             cGAGTGTCGGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGc          <  1:1403172/150‑1 (MQ=255)
                                                                                                                                             cGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGc          <  1:848540/150‑1 (MQ=255)
                                                                                                                                              gAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGCGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGcc         >  1:1689542/1‑150 (MQ=255)
                                                                                                                                              gAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGcc         <  2:1613600/150‑1 (MQ=255)
                                                                                                                                                    cAGGCGCAGATAGCCGAGCGGTCGGTTTTTACCCGCAATTGGCTCTACAATCTGCTGTTTAAATTAGCCGACTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCGTGTTc   <  2:1164160/150‑1 (MQ=255)
                                                                                                                                                    cAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTc   >  2:112597/1‑150 (MQ=255)
                                                                                                                                                     aGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCg  >  2:527970/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCG  >  NC_000913/4624210‑4624508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: