Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 492,232 T→G 5.9% R47R (CGT→CGG dnaX → DNA polymerase III/DNA elongation factor III, tau and gamma subunits

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913492,2320TG 5.9% 337.8 / 8.4 129R47R (CGT→CGGdnaXDNA polymerase III/DNA elongation factor III, tau and gamma subunits
Reads supporting (aligned to +/- strand):  ref base T (62/57);  new base G (2/6);  total (65/64)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01

Only 100 of 129 total aligned reads displayed.

GCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGACGCCGCC  >  NC_000913/492084‑492379
                                                                                                                                                    |                                                                                                                                                   
gCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTg                                                                                                                                                    <  1:1289529/150‑1 (MQ=255)
 ccTGCCAATGAGTTATCAGGTCATAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTgg                                                                                                                                                   <  1:521241/150‑1 (MQ=255)
   tGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGACCGGCTTGTCGTTAGGGCGTATTAATCATGCTTATCTTTTTTCCGGCACCCGTGGCg                                                                                                                                                 >  1:1062394/1‑150 (MQ=255)
   tGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCg                                                                                                                                                 >  2:1042915/1‑150 (MQ=255)
    gCCAATCAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTAGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGTGCGTATTCATCATGCTAATCTTTTATCCGGCACCCGTGGCGt                                                                                                                                                >  1:990252/1‑150 (MQ=255)
      cAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCg                                                                                                                                              <  2:75749/150‑1 (MQ=255)
       aaTGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCgg                                                                                                                                             <  2:1845041/150‑1 (MQ=255)
            gTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGaaaaa                                                                                                                                        >  2:102645/1‑150 (MQ=255)
            gTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGACGCCACGGGTGCCGGaaaaa                                                                                                                                        <  1:1156569/150‑1 (MQ=255)
             ttATCAGGTCTTAGCCCGTAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTTGGGCGTAATCATCATGGTTATCTTTTTTCCGGCCCCCTGGGCGTCGGAAAAAc                                                                                                                                       >  1:1283032/1‑150 (MQ=255)
              tATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAAcc                                                                                                                                      >  1:310530/1‑150 (MQ=255)
              tATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGACGCCACGGGTGCCGGaaaaaag                                                                                                                                      <  2:1117408/150‑3 (MQ=255)
              tATCAGGTCTTAGCCCGAAAATGCCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGGCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAAcc                                                                                                                                      <  1:955189/150‑1 (MQ=255)
               aTCAGGTCTTAGCCCGAAAATGGCGCCCACGAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCAGGCAAACGTGGCGTCGGAAAAACct                                                                                                                                     >  2:147204/1‑150 (MQ=255)
               aTCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGACGCCACGGGTGCCGGaaaaaaga                                                                                                                                     <  2:615358/150‑4 (MQ=255)
                 cAGGTCTTAGCCCGAAATTGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACCTGTGCTGACCGCACTGGCGATCGGCTTGTCGTGAGGGCGTATTCATCCTGCTTATCTTTTTTCCGGCTCCCGTGGGGGCGGATAACCctct                                                                                                                                   >  1:257218/1‑150 (MQ=255)
                     tcatAGCCCGAAAATGGCGACCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCg                                                                                                                               <  1:1664415/147‑1 (MQ=255)
                       ttAGCCCGAAAATGGCGCCCACAACCCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGcc                                                                                                                             <  1:356960/150‑1 (MQ=255)
                       ttAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGcc                                                                                                                             >  1:1702877/1‑150 (MQ=255)
                       ttAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGGCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGcc                                                                                                                             <  1:1696255/150‑1 (MQ=255)
                          gCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCgc                                                                                                                          >  2:646663/1‑149 (MQ=255)
                          gCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGa                                                                                                                          >  2:612114/1‑150 (MQ=255)
                           cccGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGAc                                                                                                                         >  1:1950153/1‑150 (MQ=255)
                                aaaTGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGActgctg                                                                                                                    <  2:1173617/150‑1 (MQ=255)
                                aaaTGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCAGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGActgctg                                                                                                                    <  1:1579828/150‑1 (MQ=255)
                                     gcgcCCACAACCCTTAGCTGACGTCGTCGGCCAGGAACATGTGCTGCCCGCACTGGCGAACGGCTTGTCGTGAGGGCGTAGTCATCATGCTTATCTTTTTTCCGGCAACCGTGGCGACGGAAAAACCTCTATCGCCCGACTGAAGGCGaa                                                                                                               >  2:1087573/1‑150 (MQ=255)
                                      cgcCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAg                                                                                                              <  2:628591/150‑1 (MQ=255)
                                        cccACATACCTTTGCTGTCGTCGGCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTAGTGGGTAGGGCGTATTCATCCTGGTTCTCTTTTTTCCGGCACGCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAggg                                                                                                            <  1:125987/150‑1 (MQ=255)
                                           acaAACCTTTGCTGACGTCGTCGGCCAGGAACATCTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCt                                                                                                         <  2:33857/150‑1 (MQ=255)
                                            caAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTa                                                                                                        >  2:973409/1‑150 (MQ=255)
                                              aaCCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTaaa                                                                                                      >  2:1684826/1‑150 (MQ=255)
                                              aaCCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTaaa                                                                                                      >  1:1241748/1‑150 (MQ=255)
                                                ccTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACt                                                                                                    <  1:20599/150‑1 (MQ=255)
                                                ccTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACt                                                                                                    >  1:1608389/1‑150 (MQ=255)
                                                   ttGCTGACGTCGTCGGTCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCg                                                                                                 <  2:858918/150‑1 (MQ=255)
                                                   ttGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTGCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCg                                                                                                 <  2:1963999/150‑1 (MQ=255)
                                                   ttGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGAATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCg                                                                                                 <  1:366825/150‑1 (MQ=255)
                                                     gCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGaa                                                                                               >  1:1520378/1‑150 (MQ=255)
                                                        gACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAAcc                                                                                            >  2:1284018/1‑150 (MQ=255)
                                                        gACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAAcc                                                                                            >  2:1494694/1‑150 (MQ=255)
                                                            tcgtcgGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCa                                                                                        <  1:1223832/150‑1 (MQ=255)
                                                             cgtcgGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCAt                                                                                       >  2:730276/1‑150 (MQ=255)
                                                                 ggCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCCCCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTAcc                                                                                   >  1:347350/1‑150 (MQ=255)
                                                                 ggCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTAcc                                                                                   >  1:882642/1‑150 (MQ=255)
                                                                  gCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGGTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACcg                                                                                  <  2:1616706/150‑1 (MQ=255)
                                                                     aGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGa                                                                               <  1:712165/150‑1 (MQ=255)
                                                                      ggAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAACCCGGCATTACCGCGAc                                                                              <  2:1415654/150‑1 (MQ=255)
                                                                      ggAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGAc                                                                              >  1:52122/1‑150 (MQ=255)
                                                                      ggAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGAc                                                                              >  1:1275646/1‑150 (MQ=255)
                                                                        aaCATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGc                                                                            <  2:1292635/150‑1 (MQ=255)
                                                                          cATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCg                                                                          >  2:1645558/1‑150 (MQ=255)
                                                                          cATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCg                                                                          <  2:1053828/150‑1 (MQ=255)
                                                                            tgtgCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTg                                                                        >  1:716241/1‑150 (MQ=255)
                                                                            tgtgCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTg                                                                        >  2:904579/1‑150 (MQ=255)
                                                                            tgtgCGGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGATAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTg                                                                        >  1:1199725/1‑150 (MQ=255)
                                                                              tgCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTgcg                                                                      <  1:766597/150‑1 (MQ=255)
                                                                               gCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGAACCCGTGGCGTCGGAAAACCCTCTATCGCCCGACTGCTGGCGAAGGGGCAAAACTGCGAAACCGCCATTACCGCGACGCCGTgcgg                                                                     >  2:432913/1‑150 (MQ=255)
                                                                                 tGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTCTCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTgcggcg                                                                   <  1:369673/150‑1 (MQ=255)
                                                                                  gACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCgt                                                                  >  2:1547809/1‑150 (MQ=255)
                                                                                     cGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACTCCGTGCGGCGtgtg                                                               >  1:1783380/1‑150 (MQ=255)
                                                                                           ggCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATaa                                                         >  1:1860042/1‑150 (MQ=255)
                                                                                              gaccGGCTTGTCGTTAGGGCGTATTCCTCATGCTTATCTTTTTTCCGGCCCCCGTGGCGTCGGAAAACCCTCTATCGCCCGACTGCTGGAGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTg                                                      >  1:132270/4‑150 (MQ=255)
                                                                                                 cGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCg                                                   <  1:1091571/150‑1 (MQ=255)
                                                                                                         cGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACAGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCg                                           >  1:1385154/1‑150 (MQ=255)
                                                                                                          gTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGa                                          >  2:584370/1‑150 (MQ=255)
                                                                                                           atAGGGCGTATTCATCATGCTTATCATATTTCCGGCACCCGAGGCGTCGGAAAAACCACTATCGCCCGACTGCTGGCGAAGGGTCTTAACTGCGAAACCGGCAATACCGCGACGCCGTGCGGCGTGTGCGAAAACAGTCGTGAAATCGat                                         >  2:1542879/2‑149 (MQ=255)
                                                                                                             aGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGcc                                       >  2:878833/1‑149 (MQ=255)
                                                                                                             aGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCa                                       >  1:1821614/1‑150 (MQ=255)
                                                                                                               ggCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAgg                                     >  2:1451137/1‑150 (MQ=255)
                                                                                                                   tATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGACCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGgcg                                 <  2:865610/150‑1 (MQ=255)
                                                                                                                       catcatGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCttt                             >  1:215655/1‑150 (MQ=255)
                                                                                                                       catcatGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCttt                             >  2:562193/1‑150 (MQ=255)
                                                                                                                        atcctGCTTATCTTTTTTCCGGCACCCGTGGCGGCGGAAAAACCTCTATCGCCCGACTGCTGGCGCAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTg                            <  1:148142/150‑1 (MQ=255)
                                                                                                                        atcatGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTg                            >  1:717623/1‑150 (MQ=255)
                                                                                                                           atGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCg                         <  2:1449531/150‑1 (MQ=255)
                                                                                                                            tGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGa                        <  2:546968/150‑1 (MQ=255)
                                                                                                                            tGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGa                        <  1:1103499/150‑1 (MQ=255)
                                                                                                                             gCTTATCTTTTTTCCGTCTCGCGTGTCGTCGGAAATTACTCTATCGCCCGAGTGCGGGCGAAGGGGCTAAACTTCGAAACCTGGATTACCGCGAAGCCGTGCGGCGTGTGCGCTATCTGGCGTGAAATCGAGCAGGGGCGCTTTGTCGAt                       <  2:996525/150‑1 (MQ=255)
                                                                                                                              cTTCTCTTTTTTCCGGCACCCGTGGCGTCGGAAAAAGCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATc                      <  2:1670807/150‑1 (MQ=255)
                                                                                                                              cTTATCTTTTTTGCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATc                      <  2:310530/150‑1 (MQ=255)
                                                                                                                              cTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATc                      <  2:1201637/150‑1 (MQ=255)
                                                                                                                              cTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATc                      <  2:1662634/150‑1 (MQ=255)
                                                                                                                              cTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATc                      <  1:580128/150‑1 (MQ=255)
                                                                                                                                tATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTg                    <  2:1848481/150‑1 (MQ=255)
                                                                                                                                tATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACATCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTg                    >  2:1824473/1‑150 (MQ=255)
                                                                                                                                  tCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGAt                  <  2:1365991/150‑1 (MQ=255)
                                                                                                                                  gcTTTTTCCCGGCACCCGCGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCGAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGAt                  <  1:318696/149‑1 (MQ=255)
                                                                                                                                    ttttttCCGGCACCCGTTGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTATACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTTATTg                <  2:990252/150‑1 (MQ=255)
                                                                                                                                    ttttttCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTg                <  2:1171735/150‑1 (MQ=255)
                                                                                                                                    ttttttCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTg                >  1:1506463/1‑150 (MQ=255)
                                                                                                                                     tttttCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGa               >  1:610981/1‑150 (MQ=255)
                                                                                                                                      ttttCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGaa              >  2:550788/1‑150 (MQ=255)
                                                                                                                                       tttCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGaaa             >  2:506446/1‑150 (MQ=255)
                                                                                                                                       tttCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGaaa             >  2:812282/1‑150 (MQ=255)
                                                                                                                                         tCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTTAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATc           <  2:630971/150‑1 (MQ=255)
                                                                                                                                          ccGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCg          >  2:1312967/1‑150 (MQ=255)
                                                                                                                                            ggCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGAc        >  1:941258/1‑150 (MQ=255)
                                                                                                                                             gCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGAcg       <  1:189760/150‑1 (MQ=255)
                                                                                                                                              cACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGAcgc      <  1:904579/150‑1 (MQ=255)
                                                                                                                                                  cGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGACgccgcc  <  2:1520378/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGACGCCGCC  >  NC_000913/492084‑492379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: