Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,631,639 G→C 5.8% D303H (GAT→CAT)  slt → lytic murein transglycosylase, soluble

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,631,6390GC 5.8% 460.1 / 14.6 138D303H (GAT→CAT) sltlytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand):  ref base G (69/61);  new base C (4/4);  total (73/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 145 total aligned reads displayed.

GCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAG  >  NC_000913/4631491‑4631788
                                                                                                                                                    |                                                                                                                                                     
gcgGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGAGCAGCAGCGTAATGAAGTTCAGAGTCAGGAGCTGCGCGATATAGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGa                                                                                                                                                      >  1:230010/1‑150 (MQ=255)
     aGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACg                                                                                                                                                 <  1:1071263/150‑1 (MQ=255)
      ggATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGc                                                                                                                                                >  2:1380228/1‑150 (MQ=255)
        aTGCTGAGAATGCACGGCGGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGCAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCa                                                                                                                                              <  1:507651/150‑1 (MQ=255)
           cTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTa                                                                                                                                           >  1:902043/1‑150 (MQ=255)
           cTGAGAATGCACGGCTGATCATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTa                                                                                                                                           <  1:1861118/150‑1 (MQ=255)
            tGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTAt                                                                                                                                          >  1:1089652/1‑150 (MQ=255)
             gagaATGCCCGGCTGATGAGCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATg                                                                                                                                         <  1:1591744/150‑1 (MQ=255)
             gagaATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGTCATCGCGCCAtttcg                                                                                                                                         <  2:514709/150‑4 (MQ=255)
                 aTGCACGGCTGATGATCCCATGGCGTGCCCAGGCGCAGCAGCTTAAAGCCGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAATTGGCGTCATCGCGCCAtttcgcctg                                                                                                                                     <  1:22945/150‑8 (MQ=255)
                  tGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCCCCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTc                                                                                                                                    >  2:150193/1‑150 (MQ=255)
                   gCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCg                                                                                                                                   <  2:1149128/150‑1 (MQ=255)
                   gCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCg                                                                                                                                   >  2:1251920/1‑150 (MQ=255)
                   gCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCg                                                                                                                                   <  1:315898/150‑1 (MQ=255)
                   ccACGCCTGAAGAACCCATCCCTCGCCCAGGCGCAGCAGCTAAATGAAGAGCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATCGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCg                                                                                                                                   <  1:872025/149‑1 (MQ=255)
                    cACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGc                                                                                                                                  >  2:1602736/1‑150 (MQ=255)
                              gatCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCCCCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTc                                                                                                                        >  2:699901/1‑150 (MQ=255)
                                 cccATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCt                                                                                                                     <  2:1921166/150‑1 (MQ=255)
                                 cccATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCt                                                                                                                     <  1:183429/150‑1 (MQ=255)
                                 cccATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCt                                                                                                                     >  2:830466/1‑150 (MQ=255)
                                 cccATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTGGATGGGCAACGATGTCACCGCCGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCGACTTCGCt                                                                                                                     >  1:279533/1‑150 (MQ=255)
                                  ccATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCtt                                                                                                                    >  2:1684641/1‑150 (MQ=255)
                                      cGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTg                                                                                                                <  2:1956733/150‑1 (MQ=255)
                                       gCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTgc                                                                                                               >  1:323839/1‑149 (MQ=255)
                                        cTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGAGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGaa                                                                                                              >  2:1832010/1‑150 (MQ=255)
                                            cccAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGcc                                                                                                          >  2:294065/1‑150 (MQ=255)
                                                  cgcAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTACGCTTATTGAACGCCGTGTAc                                                                                                    >  1:70227/1‑150 (MQ=255)
                                                     agcagcTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGaa                                                                                                 <  1:1186706/150‑1 (MQ=255)
                                                        agcTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATgg                                                                                              >  2:1645602/1‑150 (MQ=255)
                                                         gcTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGCACGCCGTGTACGAATGgc                                                                                             >  2:1614419/1‑150 (MQ=255)
                                                         gcTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGgc                                                                                             >  1:1556322/1‑150 (MQ=255)
                                                          cTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGgcg                                                                                            >  2:352561/1‑150 (MQ=255)
                                                          cTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGgcg                                                                                            >  2:1270462/1‑150 (MQ=255)
                                                          cTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGgcg                                                                                            >  1:870377/1‑150 (MQ=255)
                                                            tAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCt                                                                                          >  2:1043650/1‑150 (MQ=255)
                                                             aaTGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCtt                                                                                         <  1:946347/150‑1 (MQ=255)
                                                              aTGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTg                                                                                        >  1:589411/1‑150 (MQ=255)
                                                               tGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTgg                                                                                       >  1:1106107/1‑150 (MQ=255)
                                                                gAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGc                                                                                      <  2:876770/150‑1 (MQ=255)
                                                                gAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGc                                                                                      <  2:1894740/150‑1 (MQ=255)
                                                                  aGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCAc                                                                                    <  2:683377/150‑1 (MQ=255)
                                                                   gATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCAcc                                                                                   >  2:64345/1‑150 (MQ=255)
                                                                      cAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGc                                                                                >  2:1547425/1‑150 (MQ=255)
                                                                      aaGAATCAGGATCTTCGCTATATCTTAGCCTGGCGTTTGATGCGAAACGATGTCACCGACGAGCAGGCTAAATTGCGAGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCgtc                                                                                <  2:1246590/149‑3 (MQ=255)
                                                                       aGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCTCGATGAAGCCATTATGCGCTCGCAATCTACGTCGCTTATTGACCGCCTTGTACTAATGGCGCTTGGCACCGGCg                                                                               >  1:253473/1‑150 (MQ=255)
                                                                       aGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCg                                                                               <  2:736380/150‑1 (MQ=255)
                                                                       aGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCg                                                                               <  2:1652138/150‑1 (MQ=255)
                                                                        gATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGa                                                                              >  2:605957/1‑150 (MQ=255)
                                                                          ttCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTTGCACCGGCGAtc                                                                            <  2:887733/150‑1 (MQ=255)
                                                                          ttCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGAtc                                                                            <  1:707900/150‑1 (MQ=255)
                                                                           tCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGAtcg                                                                           <  1:601997/150‑1 (MQ=255)
                                                                              ggAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCGCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGAtcgtcg                                                                        <  2:92265/150‑1 (MQ=255)
                                                                                    gcgcGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCt                                                                  >  1:846874/1‑150 (MQ=255)
                                                                                    gcgcGATATCGTCGCCTGGCGTTTGAGGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCTCCGGCGATCGTCGCGGCCt                                                                  >  1:1987655/1‑150 (MQ=255)
                                                                                      gcgATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGa                                                                >  2:1461926/1‑150 (MQ=255)
                                                                                         atatCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATa                                                             <  2:996169/150‑1 (MQ=255)
                                                                                            tcgtcgCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACct                                                          <  2:1723647/150‑1 (MQ=255)
                                                                                             cgtcgCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACctg                                                         <  1:1932235/150‑1 (MQ=255)
                                                                                             cgtcgCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACATCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACctg                                                         <  1:1251920/150‑1 (MQ=255)
                                                                                                  ccTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACctggctgg                                                    >  2:1913324/1‑150 (MQ=255)
                                                                                                      gCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGcgcg                                                <  1:855847/150‑1 (MQ=255)
                                                                                                       cGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGt                                               >  2:1098636/1‑150 (MQ=255)
                                                                                                       cGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGt                                               >  1:361208/1‑150 (MQ=255)
                                                                                                       cGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGt                                               <  1:1501872/150‑1 (MQ=255)
                                                                                                         tttGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCt                                             <  2:1356832/150‑1 (MQ=255)
                                                                                                          ttGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGGGTACGAATGGCGCTTGGCACCAGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTg                                            <  1:556313/150‑1 (MQ=255)
                                                                                                            gATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGcc                                          <  1:381728/150‑1 (MQ=255)
                                                                                                            gATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGcc                                          <  2:948814/150‑1 (MQ=255)
                                                                                                                ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                      >  1:455635/1‑150 (MQ=255)
                                                                                                                ggCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATg                                      >  2:502498/1‑150 (MQ=255)
                                                                                                                    aCGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAg                                  <  2:851818/150‑1 (MQ=255)
                                                                                                                      gATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCg                                <  2:1863835/150‑1 (MQ=255)
                                                                                                                        tGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaa                              <  1:1596075/150‑1 (MQ=255)
                                                                                                                        tGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaa                              <  2:474017/150‑1 (MQ=255)
                                                                                                                         gTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaa                             <  1:648190/150‑1 (MQ=255)
                                                                                                                          tCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGCTCGTCGCGGCCTGAATGCCTGGCTGGCGCGGCTGCCTAGGGCAGCgatag                            >  1:130455/1‑150 (MQ=255)
                                                                                                                          tCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaag                            >  1:1940031/1‑150 (MQ=255)
                                                                                                                          tCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaag                            >  2:742424/1‑150 (MQ=255)
                                                                                                                             ccGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGGCTGAATACCTGGCTGGCGCGACAGCCTATGGAAGCgaaagaga                         >  2:362490/1‑150 (MQ=255)
                                                                                                                             ccGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCgaaagaga                         <  1:1168055/150‑1 (MQ=255)
                                                                                                                                  gaGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGAt                    <  2:846874/150‑1 (MQ=255)
                                                                                                                                   aGCAGGCGAAATGGCGCGATGCCGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg                   >  1:1651132/1‑150 (MQ=255)
                                                                                                                                   aGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATg                   <  1:502498/150‑1 (MQ=255)
                                                                                                                                     cAGGCGAAATGGCGCGATGACGCCATTATGCGCGCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGaa                 <  1:1380228/150‑1 (MQ=255)
                                                                                                                                      aGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCGGCCTATGGAAGCGAAAGAGAAAGATGAAt                >  2:1428068/1‑150 (MQ=255)
                                                                                                                                       ggCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATg               >  2:829163/1‑150 (MQ=255)
                                                                                                                                         cataaTGGCGTCATCGCGCCATTATGCGCTCGCAATCTACTTCGATTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAAAACCTGGCTGGCGCGTCTGCCTATGGAAGCGATAGAGTATGATGAATGGc             >  2:513578/4‑150 (MQ=255)
                                                                                                                                          gAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCg            >  2:1039647/1‑150 (MQ=255)
                                                                                                                                           taaTGGCGTCATCGCGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGt           >  1:1447525/2‑150 (MQ=255)
                                                                                                                                           taaTGGCGTCATCGCGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGt           >  1:823982/2‑150 (MQ=255)
                                                                                                                                           aaaTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGt           <  2:894898/150‑1 (MQ=255)
                                                                                                                                            aaTGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGtt          <  2:1234751/150‑1 (MQ=255)
                                                                                                                                              tGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTAc        >  1:147008/1‑150 (MQ=255)
                                                                                                                                                gcgcGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTg      >  2:1979879/1‑150 (MQ=255)
                                                                                                                                                 cgcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTgg     <  2:455635/150‑1 (MQ=255)
                                                                                                                                                 cgcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTgg     >  2:14894/1‑150 (MQ=255)
                                                                                                                                                  gcgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGc    <  1:1658280/150‑1 (MQ=255)
                                                                                                                                                  gcgATGACGCCATTATGCGCTCGCAATCAACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGGCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTAATGGc    <  2:279533/150‑1 (MQ=255)
                                                                                                                                                   cgATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCa   >  2:1461221/1‑150 (MQ=255)
                                                                                                                                                    gATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAg  <  2:853709/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGGCAGGATGCTGAGAATGCACGGCTGATGATCCCATCGCTTGCCCAGGCGCAGCAGCTTAATGAAGATCAGATTCAGGAGCTGCGCGATATCGTCGCCTGGCGTTTGATGGGCAACGATGTCACCGACGAGCAGGCGAAATGGCGCGATGACGCCATTATGCGCTCGCAATCTACTTCGCTTATTGAACGCCGTGTACGAATGGCGCTTGGCACCGGCGATCGTCGCGGCCTGAATACCTGGCTGGCGCGTCTGCCTATGGAAGCGAAAGAGAAAGATGAATGGCGTTACTGGCAG  >  NC_000913/4631491‑4631788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: