Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 494,032 A→T 6.0% intergenic (+9/‑44) dnaX → / → ybaB DNA polymerase III/DNA elongation factor III, tau and gamma subunits/DNA‑binding protein, putative nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913494,0320AT 6.0% 404.4 / 10.3 144intergenic (+9/‑44)dnaX/ybaBDNA polymerase III/DNA elongation factor III, tau and gamma subunits/DNA‑binding protein, putative nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base A (59/75);  new base T (4/4);  total (64/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.40e-01

Only 100 of 150 total aligned reads displayed.

ACGCCGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCG  >  NC_000913/493883‑494181
                                                                                                                                                     |                                                                                                                                                     
aCGCCGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGca                                                                                                                                                       <  1:211230/150‑1 (MQ=255)
aCGCCGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGca                                                                                                                                                       <  1:1849844/150‑1 (MQ=255)
 cgccgcTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGcac                                                                                                                                                      >  1:1774093/1‑150 (MQ=255)
  gccgcTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGcaca                                                                                                                                                     <  1:23201/150‑1 (MQ=255)
    cgcTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGTGGAAAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGc                                                                                                                                                   <  2:124006/150‑1 (MQ=255)
    cgcTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGc                                                                                                                                                   >  1:887432/1‑150 (MQ=255)
             ggCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCg                                                                                                                                          >  2:1668261/1‑150 (MQ=255)
             ggCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCg                                                                                                                                          <  2:1084824/150‑1 (MQ=255)
               cGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTc                                                                                                                                        <  2:608744/150‑1 (MQ=255)
                gTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCa                                                                                                                                       <  1:723255/150‑1 (MQ=255)
                gTCAGGCGATATACGAAGAAAAACTTGAGCTGGGGAGCGAGTACATTTTTTCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGAATCGGAGCTGGATGAAGATAGTATCCGCCCCATTTGATCGTAAGCACTGCTTACGTTCGTCa                                                                                                                                       <  2:1822393/150‑1 (MQ=255)
                  cAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTACGCTGTGCTTACGATcaaatgg                                                                                                                                     <  1:240495/150‑7 (MQ=255)
                  cAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCTGTGCTTACGATcaaatgg                                                                                                                                     <  2:1182968/150‑7 (MQ=255)
                  cAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGAACGTAAGCTGTGCTTACGATcaaatgg                                                                                                                                     <  1:891432/150‑7 (MQ=255)
                   aGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATcc                                                                                                                                    <  1:1331187/150‑1 (MQ=255)
                    ggCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCt                                                                                                                                   <  2:961745/150‑1 (MQ=255)
                        atatACGCATAAAAACTTTCGCAGGCGCGCGAGTCCATTATTGCGTATAATAATATTCAGACCATGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAAc                                                                                                                               <  1:728019/150‑1 (MQ=255)
                         tataCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTTTTGCGGATAATAATTTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGATGGATGAAGAATGTATCCGCCCCATTTGATCGTAAGCACTGCTTACGTTCGTCATCCTTTACg                                                                                                                              >  1:1387916/1‑150 (MQ=255)
                           taCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTg                                                                                                                            >  1:831656/1‑150 (MQ=255)
                             cGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACACTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGATCTTCATCCTTAACGTGAt                                                                                                                          >  1:1812780/1‑150 (MQ=255)
                              gaagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGAtt                                                                                                                         <  1:1488628/150‑1 (MQ=255)
                              gaagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGAtt                                                                                                                         >  1:461108/1‑150 (MQ=255)
                               aagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTg                                                                                                                        <  2:1725384/150‑1 (MQ=255)
                                agaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTga                                                                                                                       >  1:422256/1‑150 (MQ=255)
                                agaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTga                                                                                                                       >  1:524470/1‑150 (MQ=255)
                                 gaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAAGGTGATTgag                                                                                                                      >  2:457125/1‑150 (MQ=255)
                                 gaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgag                                                                                                                      >  2:605822/1‑150 (MQ=255)
                                 caaaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgag                                                                                                                      <  2:216031/149‑1 (MQ=255)
                                   aaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                    <  2:1632584/150‑1 (MQ=255)
                                   aaaaCTTGCGCAGGCGCGCGAGTACATTATTGAGTATAATAAAATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                    <  2:1801274/150‑1 (MQ=255)
                                    aaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagaga                                                                                                                   <  2:1497659/150‑1 (MQ=255)
                                         tGCGCGGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAc                                                                                                              <  2:1691394/150‑1 (MQ=255)
                                         tGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAc                                                                                                              >  2:427745/1‑150 (MQ=255)
                                           cgcAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAcat                                                                                                            >  2:433914/1‑148 (MQ=255)
                                           cgcAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGAACAGCTTACGTTCGTCATACTTAAAGTGATGGAGAGAGAAGCCt                                                                                                            >  2:1343503/1‑150 (MQ=255)
                                              aGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATg                                                                                                         <  1:884139/150‑1 (MQ=255)
                                                   ggcgAGTCCATTATTGCGGATAATAATATTCAGACCATGCGTCGGTGCTTCGATGCGGAGCTGTATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTgg                                                                                                    <  2:1812780/149‑1 (MQ=255)
                                                   cgcgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTgg                                                                                                    <  2:1047130/150‑1 (MQ=255)
                                                    gcgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGt                                                                                                   <  1:269297/150‑1 (MQ=255)
                                                     cgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTa                                                                                                  >  2:1538993/1‑150 (MQ=255)
                                                         tCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAgg                                                                                              <  1:322066/150‑1 (MQ=255)
                                                           cATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCATAAGAACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGCTAAAGGCg                                                                                            >  1:205616/1‑150 (MQ=255)
                                                            attattGCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCgg                                                                                           <  2:737808/150‑1 (MQ=255)
                                                              tattGCTGATAATAATATTCAGACCCTGCGTCTTTTCTTCGATGAGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTACGTCATCCTTAACGTTATTGAGACAGAAACCTATGTTGGGTAAAGGcagac                                                                                         >  2:362621/1‑146 (MQ=255)
                                                               attGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCt                                                                                        >  1:872361/1‑150 (MQ=255)
                                                                ttGCGGATAATAATATTCAGAGCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTg                                                                                       <  1:1272940/150‑1 (MQ=255)
                                                                 tGCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATAGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTgg                                                                                      <  2:1449543/150‑1 (MQ=255)
                                                                  ggcgaaaataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTggg                                                                                     <  2:377854/144‑1 (MQ=255)
                                                                   cGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGt                                                                                    <  1:665969/150‑1 (MQ=255)
                                                                    ggATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTTAAGGCGGTCTGGGTa                                                                                   >  1:1559615/1‑150 (MQ=255)
                                                                       taataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAAcc                                                                                <  2:780425/150‑1 (MQ=255)
                                                                        aataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCt                                                                               <  2:654180/150‑1 (MQ=255)
                                                                           aataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCtgat                                                                            <  2:1545404/150‑1 (MQ=255)
                                                                             tatTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGCCGGGCTGGGTACCCtgatga                                                                          >  1:1623435/1‑150 (MQ=255)
                                                                              atTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                                                         >  2:532280/1‑150 (MQ=255)
                                                                              atTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                                                         >  1:777758/1‑150 (MQ=255)
                                                                                 cAGACGCTGGGTCGGTTCTTCGATGCGGAGGTGGATGAAGAAAGTATCCGCCCGATTTGATCGTAAGCGCAGGTTAGGGTCGGCGTCGGTATCGGGATTGAGAGAGAGGCCTATGTGTGGTATGGGCGGGCTGGGTAAGCTGATGaagcc                                                                      >  2:666156/1‑149 (MQ=255)
                                                                                 cAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagca                                                                      >  2:757306/1‑150 (MQ=255)
                                                                                 cAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagca                                                                      >  2:1591487/1‑150 (MQ=255)
                                                                                        tGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcag                                                               >  2:1193062/1‑150 (MQ=255)
                                                                                         gCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGTACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcagc                                                              <  1:876318/150‑1 (MQ=255)
                                                                                         gCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcagc                                                              <  2:861757/150‑1 (MQ=255)
                                                                                           gTCTGTTCTTCGATTGGGAGCTGGAGGAAGAAAGTCTCCGCCCCATTTGATCGTAAGCACAGATTACTTTCGTCATCCTTTACGTGCTTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGGTACCTGATGAAGCAAGCCcagcag                                                            <  2:959263/150‑1 (MQ=255)
                                                                                              ggTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATg                                                         <  1:791680/150‑1 (MQ=255)
                                                                                                  cttcGATGCGGAGCTGGATTAAGAATGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTACCGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAg                                                     <  2:371547/150‑1 (MQ=255)
                                                                                                  cttcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAg                                                     <  2:506678/150‑1 (MQ=255)
                                                                                                     cGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGaaa                                                  <  1:565788/150‑1 (MQ=255)
                                                                                                         gctgAGCTGGATGAAGAAATTATCCGCCACATTTGATCGTAATCACAGCTTACGTTCGTCATCCTTACCGTGATTGAGAGAGAAACCTATGTTTGGTAAAGCCGGTCTGGGAAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaat                                              <  1:1943899/147‑1 (MQ=255)
                                                                                                                tGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaa                                       >  2:900178/1‑150 (MQ=255)
                                                                                                                   aTGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatg                                    >  2:494448/1‑150 (MQ=255)
                                                                                                                    tGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgc                                   <  2:777758/150‑1 (MQ=255)
                                                                                                                    tGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGACTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgc                                   <  2:1151169/150‑1 (MQ=255)
                                                                                                                    tGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCAGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgc                                   >  1:1703820/1‑150 (MQ=255)
                                                                                                                     gaagaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATTCAAGAAaaaatgcagaaaatgca                                  >  1:1201257/1‑150 (MQ=255)
                                                                                                                     gaagaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgca                                  <  2:160897/150‑1 (MQ=255)
                                                                                                                      aagaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGTGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgcag                                 <  2:1847114/150‑1 (MQ=255)
                                                                                                                       agaaAGTATCGGCCCCATTTGTTCGTAAGCGCAGCTTAGGTTCGTCATCCTTAACGTGATTGAGAGTGAAACCTATGTATGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGGAGAAAATGCTgg                                <  2:1575538/150‑1 (MQ=255)
                                                                                                                       agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAgg                                >  1:433027/1‑150 (MQ=255)
                                                                                                                       agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAgg                                >  2:1156679/1‑150 (MQ=255)
                                                                                                                       agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAgg                                >  2:1699786/1‑150 (MQ=255)
                                                                                                                         aaaGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAATGCAGAAAATGCAGGAag                             <  1:892027/150‑1 (MQ=255)
                                                                                                                             tATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAgaga                          >  1:1290887/1‑150 (MQ=255)
                                                                                                                              aTCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGAt                         >  1:1041776/1‑150 (MQ=255)
                                                                                                                               tCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATc                        >  2:1488361/1‑150 (MQ=255)
                                                                                                                                 cGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgc                      >  1:1015104/1‑150 (MQ=255)
                                                                                                                                 cGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgc                      <  1:665222/150‑1 (MQ=255)
                                                                                                                                 cGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgc                      >  2:1861790/1‑150 (MQ=255)
                                                                                                                                     ccATTTGATCGTAAGCACATCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGGCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAg                  <  2:1664915/150‑1 (MQ=255)
                                                                                                                                        tttGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGACGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTg               >  2:1242642/1‑150 (MQ=255)
                                                                                                                                         ttGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTgg              <  2:1718439/150‑1 (MQ=255)
                                                                                                                                         acgaacGTAAGCTGTGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTgg              >  2:823409/6‑150 (MQ=255)
                                                                                                                                          cgaacGTAAGCTGTGCTTACGTTCGTCATCCTGACCGTGATTGAGAGAGAAACCTATGTTTGGTAATGGCGGTCTGGGTCACCGGATGAAGCAAGCCCAGCAGATTCAAGAAAAAATGCAGATAATGCAGGAAGAGATCGCGCAGCTGGa             >  2:201847/5‑150 (MQ=255)
                                                                                                                                           gaacGTAAGCTGTGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGaa            >  2:1858411/4‑150 (MQ=255)
                                                                                                                                            aTCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAg           <  1:837545/150‑1 (MQ=255)
                                                                                                                                            aTCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAg           <  1:1610709/150‑1 (MQ=255)
                                                                                                                                            aTCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCGGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAg           <  2:872361/150‑1 (MQ=255)
                                                                                                                                              cGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTc         >  1:1145598/1‑150 (MQ=255)
                                                                                                                                              cGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTc         >  1:460396/1‑150 (MQ=255)
                                                                                                                                              cGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTc         >  1:1655548/1‑150 (MQ=255)
                                                                                                                                                    cacaGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGc   <  2:1923726/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACGCCGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCG  >  NC_000913/493883‑494181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: