Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 494,033 C→G 5.3% intergenic (+10/‑43) dnaX → / → ybaB DNA polymerase III/DNA elongation factor III, tau and gamma subunits/DNA‑binding protein, putative nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913494,0330CG 5.3% 468.3 / 11.0 144intergenic (+10/‑43)dnaX/ybaBDNA polymerase III/DNA elongation factor III, tau and gamma subunits/DNA‑binding protein, putative nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base C (60/76);  new base G (4/4);  total (64/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 149 total aligned reads displayed.

CGCCGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGA  >  NC_000913/493884‑494182
                                                                                                                                                     |                                                                                                                                                     
 gccgcTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGcaca                                                                                                                                                      <  1:23201/150‑1 (MQ=255)
   cgcTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGTGGAAAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGc                                                                                                                                                    <  2:124006/150‑1 (MQ=255)
   cgcTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGc                                                                                                                                                    >  1:887432/1‑150 (MQ=255)
           tGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCTGTGCTTACGATc                                                                                                                                            <  2:486084/150‑1 (MQ=255)
            ggCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCg                                                                                                                                           >  2:1668261/1‑150 (MQ=255)
            ggCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCg                                                                                                                                           <  1:1635599/150‑1 (MQ=255)
              cGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTc                                                                                                                                         <  2:608744/150‑1 (MQ=255)
               gTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCa                                                                                                                                        <  1:723255/150‑1 (MQ=255)
               gTCAGGCGATATACGAAGAAAAACTTGAGCTGGGGAGCGAGTACATTTTTTCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGAATCGGAGCTGGATGAAGATAGTATCCGCCCCATTTGATCGTAAGCACTGCTTACGTTCGTCa                                                                                                                                        <  2:1822393/150‑1 (MQ=255)
                 cAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTACGCTGTGCTTACGATcaaatgg                                                                                                                                      <  1:240495/150‑7 (MQ=255)
                 cAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCTGTGCTTACGATcaaatgg                                                                                                                                      <  2:1182968/150‑7 (MQ=255)
                 cAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGAACGTAAGCTGTGCTTACGATcaaatgg                                                                                                                                      <  1:891432/150‑7 (MQ=255)
                  aGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATcc                                                                                                                                     <  1:1324607/150‑1 (MQ=255)
                   ggCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCt                                                                                                                                    <  2:961745/150‑1 (MQ=255)
                       atatACGCATAAAAACTTTCGCAGGCGCGCGAGTCCATTATTGCGTATAATAATATTCAGACCATGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAAc                                                                                                                                <  1:728019/150‑1 (MQ=255)
                        tataCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTTTTGCGGATAATAATTTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGATGGATGAAGAATGTATCCGCCCCATTTGATCGTAAGCACTGCTTACGTTCGTCATCCTTTACg                                                                                                                               >  1:1387916/1‑150 (MQ=255)
                        tataCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGATCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACg                                                                                                                               >  1:160897/1‑150 (MQ=255)
                          taCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTg                                                                                                                             >  1:831656/1‑150 (MQ=255)
                            cGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACACTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGATCTTCATCCTTAACGTGAt                                                                                                                           >  1:1812780/1‑150 (MQ=255)
                             gaagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGAtt                                                                                                                          >  1:461108/1‑150 (MQ=255)
                              aagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTg                                                                                                                         <  2:1725384/150‑1 (MQ=255)
                               agaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTga                                                                                                                        >  1:524470/1‑150 (MQ=255)
                               agaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTga                                                                                                                        >  1:422256/1‑150 (MQ=255)
                                gaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgag                                                                                                                       >  2:605822/1‑150 (MQ=255)
                                gaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgag                                                                                                                       >  1:1203107/1‑150 (MQ=255)
                                caaaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgag                                                                                                                       <  2:216031/149‑1 (MQ=255)
                                  aaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                     <  1:1444061/150‑1 (MQ=255)
                                  aaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                     <  2:1632584/150‑1 (MQ=255)
                                  aaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                     <  1:1813086/150‑1 (MQ=255)
                                  aaaaCTTGCGCAGGCGCGCGAGTACATTATTGAGTATAATAAAATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                     <  2:1801274/150‑1 (MQ=255)
                                   aaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagaga                                                                                                                    <  2:1497659/150‑1 (MQ=255)
                                        tGCGCGGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAc                                                                                                               <  2:1691394/150‑1 (MQ=255)
                                        tGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAc                                                                                                               >  2:427745/1‑150 (MQ=255)
                                          cgcAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAcat                                                                                                             >  2:433914/1‑148 (MQ=255)
                                          cgcAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGAACAGCTTACGTTCGTCATACTTAAAGTGATGGAGAGAGAAGCCt                                                                                                             >  2:1343503/1‑150 (MQ=255)
                                             aGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATg                                                                                                          <  1:884139/150‑1 (MQ=255)
                                                  ggcgAGTCCATTATTGCGGATAATAATATTCAGACCATGCGTCGGTGCTTCGATGCGGAGCTGTATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTgg                                                                                                     <  2:1812780/149‑1 (MQ=255)
                                                  cgcgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTgg                                                                                                     <  2:1047130/150‑1 (MQ=255)
                                                   gcgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGt                                                                                                    <  1:269297/150‑1 (MQ=255)
                                                    cgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTa                                                                                                   >  2:1538993/1‑150 (MQ=255)
                                                        tCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAgg                                                                                               <  1:322066/150‑1 (MQ=255)
                                                          cATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCATAAGAACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGCTAAAGGCg                                                                                             >  1:205616/1‑150 (MQ=255)
                                                          cATTATTGCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCg                                                                                             <  2:1203107/150‑1 (MQ=255)
                                                           attattGCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCgg                                                                                            <  2:737808/150‑1 (MQ=255)
                                                             tattGCTGATAATAATATTCAGACCCTGCGTCTTTTCTTCGATGAGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTACGTCATCCTTAACGTTATTGAGACAGAAACCTATGTTGGGTAAAGGcagac                                                                                          >  2:362621/1‑146 (MQ=255)
                                                              attGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCt                                                                                         >  1:872361/1‑150 (MQ=255)
                                                                tGCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATAGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTgg                                                                                       <  2:1449543/150‑1 (MQ=255)
                                                                 ggcgaaaataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTggg                                                                                      <  2:377854/144‑1 (MQ=255)
                                                                  cGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGt                                                                                     <  1:665969/150‑1 (MQ=255)
                                                                   ggATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTTAAGGCGGTCTGGGTa                                                                                    >  1:1559615/1‑150 (MQ=255)
                                                                      taataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAAcc                                                                                 <  2:780425/150‑1 (MQ=255)
                                                                       aataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCt                                                                                <  2:654180/150‑1 (MQ=255)
                                                                          aataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCtgat                                                                             <  2:1545404/150‑1 (MQ=255)
                                                                            tatTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGCCGGGCTGGGTACCCtgatga                                                                           >  1:1623435/1‑150 (MQ=255)
                                                                             atTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                                                          >  2:532280/1‑150 (MQ=255)
                                                                             atTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                                                          >  1:777758/1‑150 (MQ=255)
                                                                                cAGACGCTGGGTCGGTTCTTCGATGCGGAGGTGGATGAAGAAAGTATCCGCCCGATTTGATCGTAAGCGCAGGTTAGGGTCGGCGTCGGTATCGGGATTGAGAGAGAGGCCTATGTGTGGTATGGGCGGGCTGGGTAAGCTGATGaagcc                                                                       >  2:666156/1‑149 (MQ=255)
                                                                                cAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagca                                                                       >  2:1591487/1‑150 (MQ=255)
                                                                                cAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagca                                                                       >  2:757306/1‑150 (MQ=255)
                                                                                        gCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGTACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcagc                                                               <  1:876318/150‑1 (MQ=255)
                                                                                        gCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcagc                                                               <  2:861757/150‑1 (MQ=255)
                                                                                         cGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcagca                                                              <  1:1103543/150‑1 (MQ=255)
                                                                                          gTCTGTTCTTCGATTGGGAGCTGGAGGAAGAAAGTCTCCGCCCCATTTGATCGTAAGCACAGATTACTTTCGTCATCCTTTACGTGCTTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGGTACCTGATGAAGCAAGCCcagcag                                                             <  2:959263/150‑1 (MQ=255)
                                                                                             ggTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATg                                                          <  1:791680/150‑1 (MQ=255)
                                                                                               ttcttcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCa                                                        <  1:1982787/150‑1 (MQ=255)
                                                                                                 cttcGATGCGGAGCTGGATTAAGAATGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTACCGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAg                                                      <  2:371547/150‑1 (MQ=255)
                                                                                                 cttcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAg                                                      <  2:506678/150‑1 (MQ=255)
                                                                                                    cGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGaaa                                                   <  1:565788/150‑1 (MQ=255)
                                                                                                         cGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatg                                              >  1:1711107/1‑150 (MQ=255)
                                                                                                               tGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaa                                        >  2:900178/1‑150 (MQ=255)
                                                                                                                  aTGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatg                                     >  2:494448/1‑150 (MQ=255)
                                                                                                                   tGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgc                                    <  2:777758/150‑1 (MQ=255)
                                                                                                                   tGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGACTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgc                                    <  2:1151169/150‑1 (MQ=255)
                                                                                                                   tGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCAGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgc                                    >  1:1703820/1‑150 (MQ=255)
                                                                                                                    gaagaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATTCAAGAAaaaatgcagaaaatgca                                   >  1:1201257/1‑150 (MQ=255)
                                                                                                                    gaagaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgca                                   <  2:160897/150‑1 (MQ=255)
                                                                                                                     aagaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGTGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgcag                                  <  2:1847114/150‑1 (MQ=255)
                                                                                                                      agaaAGTATCGGCCCCATTTGTTCGTAAGCGCAGCTTAGGTTCGTCATCCTTAACGTGATTGAGAGTGAAACCTATGTATGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGGAGAAAATGCTgg                                 <  2:1575538/150‑1 (MQ=255)
                                                                                                                      agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAgg                                 >  1:433027/1‑150 (MQ=255)
                                                                                                                      agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAgg                                 >  2:1699786/1‑150 (MQ=255)
                                                                                                                        aaaGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAATGCAGAAAATGCAGGAag                              <  1:892027/150‑1 (MQ=255)
                                                                                                                            tATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAgaga                           >  1:1290887/1‑150 (MQ=255)
                                                                                                                             aTCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGAt                          >  1:1041776/1‑150 (MQ=255)
                                                                                                                              tCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATc                         >  2:1488361/1‑150 (MQ=255)
                                                                                                                                cGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgc                       <  1:665222/150‑1 (MQ=255)
                                                                                                                                cGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgc                       >  2:1861790/1‑150 (MQ=255)
                                                                                                                                cGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgc                       >  1:1015104/1‑150 (MQ=255)
                                                                                                                                 gCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgcg                      <  2:1178463/150‑1 (MQ=255)
                                                                                                                                    ccATTTGATCGTAAGCACATCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGGCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAg                   <  2:1664915/150‑1 (MQ=255)
                                                                                                                                      aTTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTACCCTGATGAAGCCAGCCCAGCAGATGCAAGAAAAAATGCAGAAAAGGCAGGAAGAGATCGCGCAGcg                 >  2:1131027/1‑149 (MQ=255)
                                                                                                                                       tttGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGACGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTg                >  2:1242642/1‑150 (MQ=255)
                                                                                                                                        ttGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTgg               <  2:1718439/150‑1 (MQ=255)
                                                                                                                                        acgaacGTAAGCTGTGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTgg               >  2:823409/6‑150 (MQ=255)
                                                                                                                                         cgaacGTAAGCTGTGCTTACGTTCGTCATCCTGACCGTGATTGAGAGAGAAACCTATGTTTGGTAATGGCGGTCTGGGTCACCGGATGAAGCAAGCCCAGCAGATTCAAGAAAAAATGCAGATAATGCAGGAAGAGATCGCGCAGCTGGa              >  2:201847/5‑150 (MQ=255)
                                                                                                                                          gaacGTAAGCTGTGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGaa             >  2:1858411/4‑150 (MQ=255)
                                                                                                                                           aTCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAg            <  1:837545/150‑1 (MQ=255)
                                                                                                                                           aTCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCGGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAg            <  2:872361/150‑1 (MQ=255)
                                                                                                                                             cGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTc          >  1:460396/1‑150 (MQ=255)
                                                                                                                                                   cacaGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGc    <  2:1923726/150‑1 (MQ=255)
                                                                                                                                                     caGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGa  >  1:986709/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCCGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGA  >  NC_000913/493884‑494182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: