Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 494,034 A→T 5.8% intergenic (+11/‑42) dnaX → / → ybaB DNA polymerase III/DNA elongation factor III, tau and gamma subunits/DNA‑binding protein, putative nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913494,0340AT 5.8% 411.4 / 5.6 144intergenic (+11/‑42)dnaX/ybaBDNA polymerase III/DNA elongation factor III, tau and gamma subunits/DNA‑binding protein, putative nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base A (59/75);  new base T (5/5);  total (64/80)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

GCCGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGA  >  NC_000913/493885‑494182
                                                                                                                                                     |                                                                                                                                                    
          tGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCTGTGCTTACGATc                                                                                                                                            <  2:486084/150‑1 (MQ=255)
           ggCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGCCCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCg                                                                                                                                           >  1:883610/1‑150 (MQ=255)
           ggCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCg                                                                                                                                           <  2:1084824/150‑1 (MQ=255)
           ggCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCg                                                                                                                                           <  1:1635599/150‑1 (MQ=255)
           ggCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATACGCCCCATTTGATCGTAAGCACAGCTTACGTTCg                                                                                                                                           >  1:364714/1‑150 (MQ=255)
             cGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTc                                                                                                                                         <  2:608744/150‑1 (MQ=255)
              gTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCa                                                                                                                                        <  1:723255/150‑1 (MQ=255)
              gTCAGGCGATATACGAAGAAAAACTTGAGCTGGGGAGCGAGTACATTTTTTCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGAATCGGAGCTGGATGAAGATAGTATCCGCCCCATTTGATCGTAAGCACTGCTTACGTTCGTCa                                                                                                                                        <  2:1822393/150‑1 (MQ=255)
                cAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTACGCTGTGCTTACGATcaaatgg                                                                                                                                      <  1:240495/150‑7 (MQ=255)
                cAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGAACGTAAGCTGTGCTTACGATcaaatgg                                                                                                                                      <  1:891432/150‑7 (MQ=255)
                 aGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATcc                                                                                                                                     <  1:1324607/150‑1 (MQ=255)
                  ggCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCt                                                                                                                                    <  2:961745/150‑1 (MQ=255)
                       tataCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTTTTGCGGATAATAATTTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGATGGATGAAGAATGTATCCGCCCCATTTGATCGTAAGCACTGCTTACGTTCGTCATCCTTTACg                                                                                                                               >  1:1387916/1‑150 (MQ=255)
                       tataCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGATCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACg                                                                                                                               >  1:160897/1‑150 (MQ=255)
                         taCGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTg                                                                                                                             >  1:831656/1‑150 (MQ=255)
                            gaagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGAtt                                                                                                                          >  1:461108/1‑150 (MQ=255)
                             aagaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTg                                                                                                                         <  2:1817539/150‑1 (MQ=255)
                              agaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTga                                                                                                                        >  1:524470/1‑150 (MQ=255)
                               gaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgag                                                                                                                       >  1:1203107/1‑150 (MQ=255)
                               gaaAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgag                                                                                                                       >  2:605822/1‑150 (MQ=255)
                               caaaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgag                                                                                                                       <  2:216031/149‑1 (MQ=255)
                                aaaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgaga                                                                                                                      <  1:887447/150‑1 (MQ=255)
                                 aaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                     <  1:1813086/150‑1 (MQ=255)
                                 aaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                     <  1:1444061/150‑1 (MQ=255)
                                 aaaaCTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                     <  2:215958/150‑1 (MQ=255)
                                 aaaaCTTGCGCAGGCGCGCGAGTACATTATTGAGTATAATAAAATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTgagag                                                                                                                     <  2:1801274/150‑1 (MQ=255)
                                       tGCGCGGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAc                                                                                                               <  2:1691394/150‑1 (MQ=255)
                                       tGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAc                                                                                                               >  2:1253858/1‑150 (MQ=255)
                                       tGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAc                                                                                                               >  2:427745/1‑150 (MQ=255)
                                        gcgcAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTAGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAca                                                                                                              >  2:1672556/1‑149 (MQ=255)
                                         cgcAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAAcat                                                                                                             >  2:433914/1‑148 (MQ=255)
                                         cgcAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGAACAGCTTACGTTCGTCATACTTAAAGTGATGGAGAGAGAAGCCt                                                                                                             >  2:1343503/1‑150 (MQ=255)
                                            aGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATg                                                                                                          <  1:884139/150‑1 (MQ=255)
                                                   cgAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTa                                                                                                   >  2:1538993/1‑150 (MQ=255)
                                                       tCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAgg                                                                                               <  1:322066/150‑1 (MQ=255)
                                                         cATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCg                                                                                             <  1:221085/150‑1 (MQ=255)
                                                         cATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCATAAGAACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGCTAAAGGCg                                                                                             >  1:205616/1‑150 (MQ=255)
                                                         cATTATTGCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCg                                                                                             <  2:1203107/150‑1 (MQ=255)
                                                          attattGCGGATAATAATATTCAGACCCTGCGGCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCgg                                                                                            <  2:737808/150‑1 (MQ=255)
                                                            tattGCTGATAATAATATTCAGACCCTGCGTCTTTTCTTCGATGAGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTACGTCATCCTTAACGTTATTGAGACAGAAACCTATGTTGGGTAAAGGcagac                                                                                          >  2:362621/1‑146 (MQ=255)
                                                             attGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCt                                                                                         >  1:872361/1‑150 (MQ=255)
                                                              ttGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTg                                                                                        <  1:682259/150‑1 (MQ=255)
                                                                ggcgaaaataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTggg                                                                                      <  2:377854/144‑1 (MQ=255)
                                                                  ggATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTTAAGGCGGTCTGGGTa                                                                                    >  1:1559615/1‑150 (MQ=255)
                                                                     taataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAAcc                                                                                 <  2:780425/150‑1 (MQ=255)
                                                                      aataataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCt                                                                                <  2:654180/150‑1 (MQ=255)
                                                                         aataTTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCtgat                                                                             <  1:944121/150‑1 (MQ=255)
                                                                           tatTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCtgatga                                                                           <  1:741105/150‑1 (MQ=255)
                                                                           tatTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGCCGGGCTGGGTACCCtgatga                                                                           >  1:1623435/1‑150 (MQ=255)
                                                                            atTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                                                          >  2:532280/1‑150 (MQ=255)
                                                                            atTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                                                          >  1:777758/1‑150 (MQ=255)
                                                                            atTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaa                                                                          >  1:851989/1‑150 (MQ=255)
                                                                              tCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCTGTCTGGGTAACCTGATGaagc                                                                        <  2:1774093/150‑1 (MQ=255)
                                                                               cAGACGCTGGGTCGGTTCTTCGATGCGGAGGTGGATGAAGAAAGTATCCGCCCGATTTGATCGTAAGCGCAGGTTAGGGTCGGCGTCGGTATCGGGATTGAGAGAGAGGCCTATGTGTGGTATGGGCGGGCTGGGTAAGCTGATGaagcc                                                                       >  2:666156/1‑149 (MQ=255)
                                                                               cAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGaagca                                                                       >  2:757306/1‑150 (MQ=255)
                                                                                    ccTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCTGTCTGGGTAACCTGATGAAGCAAGccc                                                                  >  1:252857/1‑150 (MQ=255)
                                                                                    ccTGCGGCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATACGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGccc                                                                  <  1:820173/150‑1 (MQ=255)
                                                                                      tGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcag                                                                >  2:1193062/1‑150 (MQ=255)
                                                                                       gCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcagc                                                               <  2:861757/150‑1 (MQ=255)
                                                                                        cGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCcagca                                                              <  1:1103543/150‑1 (MQ=255)
                                                                                        cGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGATCTGGGTAACCTGATGAAGCAAGCCcagca                                                              <  1:490308/150‑1 (MQ=255)
                                                                                         gTCTGTTCTTCGATTGGGAGCTGGAGGAAGAAAGTCTCCGCCCCATTTGATCGTAAGCACAGATTACTTTCGTCATCCTTTACGTGCTTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGGTACCTGATGAAGCAAGCCcagcag                                                             <  2:959263/150‑1 (MQ=255)
                                                                                           cGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGAt                                                           <  2:1866371/150‑1 (MQ=255)
                                                                                              ttcttcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCa                                                        <  2:1715313/150‑1 (MQ=255)
                                                                                               tcttcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCaa                                                       >  2:1633958/1‑150 (MQ=255)
                                                                                                cttcGATGCGGAGCTGGATTAAGAATGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTACCGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAg                                                      <  2:371547/150‑1 (MQ=255)
                                                                                                cttcGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAg                                                      <  2:506678/150‑1 (MQ=255)
                                                                                                       gctgAGCTGGATGAAGAAATTATCCGCCACATTTGATCGTAATCACAGCTTACGTTCGTCATCCTTACCGTGATTGAGAGAGAAACCTATGTTTGGTAAAGCCGGTCTGGGAAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaat                                               <  1:1943899/147‑1 (MQ=255)
                                                                                                        cGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatg                                              <  2:1563747/150‑1 (MQ=255)
                                                                                                        cGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatg                                              >  1:1711107/1‑150 (MQ=255)
                                                                                                         ggAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgc                                             <  2:1602036/150‑1 (MQ=255)
                                                                                                         cgAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgc                                             >  1:44408/2‑150 (MQ=255)
                                                                                                              tGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaa                                        >  2:900178/1‑150 (MQ=255)
                                                                                                                 aTGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatg                                     >  2:494448/1‑150 (MQ=255)
                                                                                                                  tGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgc                                    <  2:777758/150‑1 (MQ=255)
                                                                                                                  tGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCAGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgc                                    >  1:1703820/1‑150 (MQ=255)
                                                                                                                   gaagaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATTCAAGAAaaaatgcagaaaatgca                                   >  1:1201257/1‑150 (MQ=255)
                                                                                                                   gaagaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAaaaatgcagaaaatgca                                   <  2:160897/150‑1 (MQ=255)
                                                                                                                     agaaAGTATCGGCCCCATTTGTTCGTAAGCGCAGCTTAGGTTCGTCATCCTTAACGTGATTGAGAGTGAAACCTATGTATGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGGAGAAAATGCTgg                                 <  2:1575538/150‑1 (MQ=255)
                                                                                                                     agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAgg                                 >  2:1156679/1‑150 (MQ=255)
                                                                                                                     agaaAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAgg                                 >  1:433027/1‑150 (MQ=255)
                                                                                                                           tATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAgaga                           >  1:1290887/1‑150 (MQ=255)
                                                                                                                            aTCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGAt                          >  1:1041776/1‑150 (MQ=255)
                                                                                                                             tCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATc                         >  2:1488361/1‑150 (MQ=255)
                                                                                                                               cGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgc                       >  1:1015104/1‑150 (MQ=255)
                                                                                                                               cGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgc                       <  1:665222/150‑1 (MQ=255)
                                                                                                                                gCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATcgcg                      <  2:1178463/150‑1 (MQ=255)
                                                                                                                                   ccATTTGATCGTAAGCACATCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGGCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAg                   <  2:1664915/150‑1 (MQ=255)
                                                                                                                                     aTTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTACCCTGATGAAGCCAGCCCAGCAGATGCAAGAAAAAATGCAGAAAAGGCAGGAAGAGATCGCGCAGcg                 >  2:1131027/1‑149 (MQ=255)
                                                                                                                                      tttGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGACGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTg                >  2:1242642/1‑150 (MQ=255)
                                                                                                                                       ttGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTgg               <  2:1718439/150‑1 (MQ=255)
                                                                                                                                       acgaacGTAAGCTGTGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTgg               >  2:823409/6‑150 (MQ=255)
                                                                                                                                         gaacGTAAGCTGTGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGaa             >  2:1850722/4‑150 (MQ=255)
                                                                                                                                         gaacGTAAGCTGTGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGaa             >  2:1858411/4‑150 (MQ=255)
                                                                                                                                          aTCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCGGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAg            <  2:872361/150‑1 (MQ=255)
                                                                                                                                            cGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAACCCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGATAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTc          >  2:146134/1‑150 (MQ=255)
                                                                                                                                            cGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGAGTGAGAGAGAAACCTATGTTTGGGAAAGGCGGTCTGGGTAACCTGATGACGCTCGCCCCGCAGATGCAAGAAAAAATGCAGAAATTGCAGGAAGAGATCGCGCAGCTGGAAggc          >  2:1522416/1‑148 (MQ=255)
                                                                                                                                                  cacaGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGc    <  2:1923726/150‑1 (MQ=255)
                                                                                                                                                   acaGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCg   <  1:605822/150‑1 (MQ=255)
                                                                                                                                                    caGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGa  >  1:986709/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCGCTGGAGTGGCGTCAGGCGATATACGAAGAAAAACTTGCGCAGGCGCGCGAGTCCATTATTGCGGATAATAATATTCAGACCCTGCGTCGGTTCTTCGATGCGGAGCTGGATGAAGAAAGTATCCGCCCCATTTGATCGTAAGCACAGCTTACGTTCGTCATCCTTAACGTGATTGAGAGAGAAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGA  >  NC_000913/493885‑494182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: