Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,019 T→A 5.3% intergenic (+23/+13) cysS → / ← ybcI cysteinyl‑tRNA synthetase/DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,0190TA 5.3% 364.9 / 3.9 126intergenic (+23/+13)cysS/ybcIcysteinyl‑tRNA synthetase/DUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (53/64);  new base A (3/4);  total (56/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

TCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCT  >  NC_000913/555870‑556168
                                                                                                                                                     |                                                                                                                                                     
tCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGt                                                                                                                                                       >  1:871799/1‑150 (MQ=255)
 cAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGCCCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGtt                                                                                                                                                      >  2:87897/1‑150 (MQ=255)
  aaCAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGttt                                                                                                                                                     >  2:2012894/1‑150 (MQ=255)
     cgCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCg                                                                                                                                                  <  1:69527/149‑1 (MQ=255)
     aGCGTCTGGATGCCCGTAAAGCGAAAGCCTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCg                                                                                                                                                  <  2:1192590/150‑1 (MQ=255)
       cGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGGGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCa                                                                                                                                                <  2:1588650/150‑1 (MQ=255)
          cTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATcc                                                                                                                                             <  1:1929069/150‑1 (MQ=255)
           tGGATGCCCGTAAAGCGAACGACTGGGCGGCGGCGGATGCGGCGCGGGAGCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCg                                                                                                                                            <  2:1079162/150‑1 (MQ=255)
            ggATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCgg                                                                                                                                           <  2:235375/150‑1 (MQ=255)
             gATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGt                                                                                                                                          >  1:115938/1‑150 (MQ=255)
             gATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCggc                                                                                                                                          <  1:742968/150‑2 (MQ=255)
               tGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGAGCGTCTTAACGAGATGGGGAGCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCggcaa                                                                                                                                        <  1:1874200/150‑4 (MQ=255)
                 cccGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATc                                                                                                                                      >  1:588786/1‑150 (MQ=255)
                  ccGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCg                                                                                                                                     <  2:1728079/150‑1 (MQ=255)
                  ccGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGGGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCg                                                                                                                                     <  1:449376/150‑1 (MQ=255)
                   cGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTTGCATCCGGTTATCGt                                                                                                                                    >  2:1381782/1‑150 (MQ=255)
                        aGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGAAGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGTCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcg                                                                                                                               >  1:1209099/1‑150 (MQ=255)
                        aGCGAAAGACTGGGCGGCGGCGGAGGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcg                                                                                                                               <  1:1518757/150‑1 (MQ=255)
                         gCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcgc                                                                                                                              >  2:1519120/1‑150 (MQ=255)
                           gAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                            <  2:542544/150‑1 (MQ=255)
                               gACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCcaccac                                                                                                                        >  1:934890/1‑150 (MQ=255)
                                aCTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACcaca                                                                                                                       <  1:483295/150‑1 (MQ=255)
                                      cggcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTc                                                                                                                 <  2:869860/150‑1 (MQ=255)
                                       ggcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTcc                                                                                                                <  1:1417502/150‑1 (MQ=255)
                                       ggcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTcc                                                                                                                <  2:1747545/150‑1 (MQ=255)
                                       ggcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTcc                                                                                                                <  2:666222/150‑1 (MQ=255)
                                        gcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTccc                                                                                                               <  2:1404105/150‑1 (MQ=255)
                                        gcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTccc                                                                                                               >  2:1647295/1‑150 (MQ=255)
                                         cggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCa                                                                                                              <  1:1208164/150‑1 (MQ=255)
                                            cggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCa                                                                                                           >  2:545750/1‑150 (MQ=255)
                                             ggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAg                                                                                                          <  2:1391043/150‑1 (MQ=255)
                                             ggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAg                                                                                                          <  2:271096/150‑1 (MQ=255)
                                             ggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAg                                                                                                          <  1:19840/150‑1 (MQ=255)
                                              gATGCGGCGCGTGANCGNCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGt                                                                                                         <  1:1772943/150‑1 (MQ=255)
                                                tGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAg                                                                                                       <  2:1532031/150‑1 (MQ=255)
                                                  cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCATGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                     <  1:29915/150‑1 (MQ=255)
                                                   ggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCAt                                                                                                    <  1:443313/150‑1 (MQ=255)
                                                   ggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTCGCGTCGTAAGTAATTGCGCTATTGCCCGATGCGAGTTTTCGCATCCGCTTATCGTCTCCGCCACCCCAACATTCCCCTCAGTGGctt                                                                                                    >  2:689574/1‑148 (MQ=255)
                                                     cgcgTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATcc                                                                                                  >  2:1204873/1‑150 (MQ=255)
                                                        gTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTGGCATCCCCg                                                                                               <  2:464787/150‑1 (MQ=255)
                                                         tGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCCTCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCgg                                                                                              >  1:910655/1‑150 (MQ=255)
                                                         tGATCCTCTTAACGAGAGGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCgg                                                                                              <  2:1276362/150‑1 (MQ=255)
                                                         tGAGCGTCTTAACGAGAGGGGGATCGTGCTGGAAGATGGCCCGCTAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCTGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCgg                                                                                              <  2:1492482/150‑1 (MQ=255)
                                                          gATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGc                                                                                             >  2:1835667/1‑150 (MQ=255)
                                                              gTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACACCATTCCCATCAGTAGCATCCCCGGCAAcc                                                                                         >  1:393812/1‑150 (MQ=255)
                                                                  tAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCacac                                                                                     >  2:45350/1‑150 (MQ=255)
                                                                   aaCGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGACGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAcc                                                                                    <  1:224370/150‑1 (MQ=255)
                                                                     cGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCca                                                                                  >  1:598344/1‑150 (MQ=255)
                                                                        gaTGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTACCATCAGTAGCATCCCCGGCAACCACTCCcacag                                                                               >  2:290550/1‑149 (MQ=255)
                                                                          tGGGGATCGTGCTGGAAGATGGCCCGCAAGGGTCCACCTTGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCa                                                                             <  1:1270924/150‑1 (MQ=255)
                                                                             ggATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAAtt                                                                          >  1:737934/1‑150 (MQ=255)
                                                                             ggATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGGTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCTAtt                                                                          >  1:1958510/1‑150 (MQ=255)
                                                                             ggATCGTGCAGGATGATGGCCCGCAAGGGACCACCTTGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAAtt                                                                          <  2:1240880/150‑1 (MQ=255)
                                                                              gATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTc                                                                         <  2:644293/150‑1 (MQ=255)
                                                                               aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCa                                                                        <  1:1393789/150‑1 (MQ=255)
                                                                               aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTGTTCGCATCCGGTGATCGTCTGCGCCACCACAACATTCCCCTCAGTAGCATCCCCGGCACCCACCGCCACATCAAGTCa                                                                        >  1:82065/1‑150 (MQ=255)
                                                                                 cgggCTGGAAGATGGCCGGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAAATCAGa                                                                      <  2:378618/147‑1 (MQ=255)
                                                                                 cGTGCTGGAAGATGGCCCGCAAGTGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGa                                                                      <  2:1666120/150‑1 (MQ=255)
                                                                                 cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGa                                                                      <  1:973251/150‑1 (MQ=255)
                                                                                    gCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCCCATCAATTCAGAaat                                                                   >  2:291655/1‑150 (MQ=255)
                                                                                    gCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                   <  1:613543/150‑1 (MQ=255)
                                                                                      tGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCCCCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataa                                                                 >  1:500725/1‑150 (MQ=255)
                                                                                            aTGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTTTTGCCGGATGCGAGTTTTCGGATCCGGTTATCGAATGCGCCAACACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGATAGAATAACCt                                                           >  1:366393/1‑150 (MQ=255)
                                                                                               gCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGAt                                                        >  2:1888681/1‑150 (MQ=255)
                                                                                               gCCCGCAAGGGACCACCTGGCGGAGTCAGTAATTGCGCTATTGCCGTATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGAATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGAt                                                        <  2:146927/150‑1 (MQ=255)
                                                                                                cccGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATg                                                       <  1:928837/150‑1 (MQ=255)
                                                                                                 ccGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGc                                                      >  2:1632968/1‑150 (MQ=255)
                                                                                                 ccGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGATTTCGCATCCGGTTATCGTCTGCGCCACCACAACAATACCATCAGTAGCATCCCCGGCAACCCCACCCAAATCAATTCAGAAATAATCACCTGATGc                                                      >  1:1062057/1‑150 (MQ=255)
                                                                                                       gggCCCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCAACCGGTTATCGTCTGCGCCACAACAACATTCCCATCAGTAGCATCCCCGGCAACCCCACCCACATCAATTCAGAAATAATCGCCGGATGCCCGTAc                                                >  2:193222/1‑150 (MQ=255)
                                                                                                       gggACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGAAGCCCGTAc                                                >  2:404407/1‑150 (MQ=255)
                                                                                                        ggACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAAGTCAGAAATAATCACCTGATGCCCGTACg                                               >  1:1512014/1‑150 (MQ=255)
                                                                                                            caccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgt                                           <  1:982581/150‑1 (MQ=255)
                                                                                                             agcTGGCGTCGTAAGTAATTGCGCTATTGCCGTATGCGAGTTTTAGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtg                                          <  2:344823/148‑1 (MQ=255)
                                                                                                              ccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtgg                                         <  1:1793961/150‑1 (MQ=255)
                                                                                                               cTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGCTGCCCGTACGGCgtggt                                        >  2:626580/1‑150 (MQ=255)
                                                                                                                tGGCGTCGGAAGTAATTGCCGTATTGCCGGATGCGAGCGTTCGCATCCGGTTATCGTCTGCGCCACCACAACCTTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAAATCAGAAATAATCCCCTGATGCCCGTACGGCgtgggg                                       <  2:348478/150‑3 (MQ=255)
                                                                                                                tGGCGGCGGAAGTAATTGCGCTATTGACGGATGCGAGTTTTCGCATCCTGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCGCGGCAACCACACCCACATCAATTCTGAAATAATCACCTGATGTCCGTACGGCgtggtg                                       <  1:113809/150‑1 (MQ=255)
                                                                                                                 ggCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCGCAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGgtgt                                      >  1:206475/1‑150 (MQ=255)
                                                                                                                  gCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTa                                     <  1:2012894/150‑1 (MQ=255)
                                                                                                                   cgtcgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGCAATAATCACCTGATGCCCGTACGGCGGGGGGTaa                                    >  2:1696382/1‑150 (MQ=255)
                                                                                                                    gtcgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAAc                                   >  1:385353/1‑150 (MQ=255)
                                                                                                                     tcgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACg                                  <  2:1566570/150‑1 (MQ=255)
                                                                                                                       gtaagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgag                                <  1:505278/150‑1 (MQ=255)
                                                                                                                         aagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGAc                              <  1:1007091/150‑1 (MQ=255)
                                                                                                                         aagtaaTTGCGCTATTGACGGATGCGAGTTTTCGCATCCGTTTATCGTCTGCGCCACCACACCATTCCCATCAGTAGCATCCCCAGCAAACAACCCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCTGGGTGTAACGCGAc                              >  2:292268/1‑150 (MQ=255)
                                                                                                                          agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACCCCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                             >  1:1628403/1‑150 (MQ=255)
                                                                                                                          agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                             >  2:1820821/1‑150 (MQ=255)
                                                                                                                           gtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACaa                            >  2:654469/1‑150 (MQ=255)
                                                                                                                               ttGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCa                        <  1:87897/150‑1 (MQ=255)
                                                                                                                                       ttGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGgcgc                <  1:1430759/150‑1 (MQ=255)
                                                                                                                                        tGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCATAAATAAACACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGcgcg               >  2:1008602/1‑150 (MQ=255)
                                                                                                                                          ccGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTTATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGAc             >  2:1495354/1‑150 (MQ=255)
                                                                                                                                             gATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACttt          <  1:1662503/150‑1 (MQ=255)
                                                                                                                                              aTGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCTGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACtttt         <  1:238997/150‑1 (MQ=255)
                                                                                                                                               tGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCGCCGGCTACCTCACCCACATGAATTCAGATATAATCACCTGATGCCCGTACGGCGGGGTGTAACGAGACATTGCAAACGGCGCGACTTTTa        <  2:1812910/150‑1 (MQ=255)
                                                                                                                                                gCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTAt       <  1:1596686/150‑1 (MQ=255)
                                                                                                                                                 cGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCAATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGAGTAACGAGACAATGAAAACGGCGCGACTTTTATc      >  1:1057884/1‑150 (MQ=255)
                                                                                                                                                    gTTTTCGCATCCGGTTTTCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACCCCCACATCCAGTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAATCGGCGCGACTTTTAGCAcc   >  1:1752097/1‑150 (MQ=255)
                                                                                                                                                    gTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAcc   <  1:1295049/150‑1 (MQ=255)
                                                                                                                                                     ttttCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGCGACAATGCAAACGGCGCGACTTTTTTccccg  >  2:764932/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCT  >  NC_000913/555870‑556168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: