Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 632,134 C→A 5.8% intergenic (+135/‑48) cstA → / → ybdD carbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913632,1340CA 5.8% 366.1 / 8.5 120intergenic (+135/‑48)cstA/ybdDcarbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein
Reads supporting (aligned to +/- strand):  ref base C (64/48);  new base A (4/4);  total (68/52)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 122 total aligned reads displayed.

AAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGT  >  NC_000913/631985‑632282
                                                                                                                                                     |                                                                                                                                                    
aaaGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTc                                                                                                                                                      <  1:1782253/150‑1 (MQ=255)
aaaGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGGGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTc                                                                                                                                                      <  1:1571642/150‑1 (MQ=255)
   ggCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcg                                                                                                                                                   >  1:754976/1‑150 (MQ=255)
   ggCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcg                                                                                                                                                   >  1:1461315/1‑150 (MQ=255)
     cgcACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGGGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                                                                                                 >  1:1272802/1‑150 (MQ=255)
      gcACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcgccg                                                                                                                                                >  2:1062220/1‑150 (MQ=255)
      gcACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcgccg                                                                                                                                                <  1:1423733/150‑1 (MQ=255)
           cTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGATCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                           <  1:1380614/150‑1 (MQ=38)
            aaaaGGCAGCGGGCGGGGGGGATGTTGGCGAATGCTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                                          <  1:1994376/149‑1 (MQ=39)
              aaGTCAGCGGGCGGGGGGGATGTTGGCGACTGTTGGCGTAGGGCCCAGGGGTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                        <  1:369583/150‑1 (MQ=37)
              aaGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                        <  2:1178282/150‑1 (MQ=39)
                  cAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGGGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGcctac                                                                                                                                    <  1:1481474/150‑5 (MQ=38)
                       ggAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCa                                                                                                                               <  2:75109/149‑1 (MQ=255)
                         aGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGc                                                                                                                             <  2:1127846/150‑1 (MQ=255)
                            gggggATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAAc                                                                                                                          <  1:1781316/150‑1 (MQ=255)
                                  tGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATACGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGtt                                                                                                                    >  1:1237475/1‑150 (MQ=255)
                                  tGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGtt                                                                                                                    >  1:1912064/1‑150 (MQ=255)
                                     tGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGc                                                                                                                 >  2:1304997/1‑150 (MQ=255)
                                       gCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCgg                                                                                                               <  1:1109919/150‑1 (MQ=255)
                                          aaTGTTGGCTTAGTGCCGAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGGTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAgg                                                                                                            <  2:1807325/150‑1 (MQ=255)
                                            tGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGcc                                                                                                          <  2:1212643/150‑1 (MQ=255)
                                                   ttAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGt                                                                                                   <  1:1650464/150‑1 (MQ=255)
                                                    tAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGtt                                                                                                  <  2:1461315/150‑1 (MQ=255)
                                                    tAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGtt                                                                                                  <  2:754976/150‑1 (MQ=255)
                                                    tAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACGCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGtt                                                                                                  >  1:1681726/1‑150 (MQ=255)
                                                    tAGGGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGTATAAGGCGTTCACGCCGCATCAGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGtt                                                                                                  <  1:1227075/150‑1 (MQ=255)
                                                        gCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGAt                                                                                              <  1:1543842/150‑1 (MQ=255)
                                                         cccAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGAtt                                                                                             >  2:1611930/1‑150 (MQ=255)
                                                         cccAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGAtt                                                                                             <  1:1462999/150‑1 (MQ=255)
                                                          ccAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTc                                                                                            <  2:1390906/150‑1 (MQ=255)
                                                          ccAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTc                                                                                            <  2:1950398/150‑1 (MQ=255)
                                                            aGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCAc                                                                                          >  1:1607968/1‑150 (MQ=255)
                                                             gggTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATATCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACt                                                                                         <  1:1025594/150‑1 (MQ=255)
                                                             gggTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACt                                                                                         >  1:1642187/1‑150 (MQ=255)
                                                                ttCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTTACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGc                                                                                      <  1:689665/150‑1 (MQ=255)
                                                                  cccTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCaa                                                                                    >  2:921490/1‑150 (MQ=255)
                                                                  cccTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGAGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCaa                                                                                    >  2:1604650/1‑150 (MQ=255)
                                                                    ctctCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCaaaa                                                                                  >  2:350881/1‑150 (MQ=255)
                                                                        cACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCg                                                                              >  2:863155/1‑150 (MQ=255)
                                                                         aCCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCgg                                                                             <  1:464092/150‑1 (MQ=255)
                                                                          cccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGa                                                                            >  2:173564/1‑150 (MQ=255)
                                                                          cccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGa                                                                            >  1:1770875/1‑150 (MQ=255)
                                                                          cccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGa                                                                            >  2:872075/1‑150 (MQ=255)
                                                                          cccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGa                                                                            >  1:560834/1‑150 (MQ=255)
                                                                           ccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGaa                                                                           <  1:1496087/150‑1 (MQ=255)
                                                                             tAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGaaaa                                                                         >  1:1109175/1‑150 (MQ=255)
                                                                             gtccccTCGCCCCACCGGGGAGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGaaaa                                                                         >  1:1583709/4‑150 (MQ=39)
                                                                               ccccTCGCCCCACCGGGGAGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGCCAAAAGCCCGAAAata                                                                       >  2:1992027/2‑150 (MQ=38)
                                                                                 ccTCTCCCCGGTGGGGCGAAGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACAGGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATAtt                                                                     >  2:919712/1‑150 (MQ=255)
                                                                                   tctcCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTa                                                                   <  2:473716/150‑1 (MQ=255)
                                                                                    ctcCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAg                                                                  >  1:245608/1‑150 (MQ=255)
                                                                                      ccccGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACCGGCAAAAGCCGGAAAATATTTAGGt                                                                >  2:934375/1‑150 (MQ=255)
                                                                                       cccGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTc                                                               >  1:1966186/1‑150 (MQ=255)
                                                                                              gggCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCTCTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcggc                                                        >  1:142458/1‑150 (MQ=255)
                                                                                              gggCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcggc                                                        <  2:1116763/150‑1 (MQ=255)
                                                                                              gggCGAGGGGACTGACCGAGCGCGGTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcggc                                                        <  2:767434/150‑1 (MQ=255)
                                                                                               ggCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATACGGCGGTCACGCCGCATCCGGCACTCTTTCAGCAACATGGGTAGCGGAGGCCAAGATGTTTGATTCTCTGGCAAAAGCCGGAAAATAGTTAGGTCAGgcggcg                                                       >  1:307929/1‑150 (MQ=255)
                                                                                                       gACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgat                                               >  2:501686/1‑150 (MQ=255)
                                                                                                         cTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                             >  1:611960/1‑150 (MQ=255)
                                                                                                         cTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGAATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                             <  1:266442/150‑1 (MQ=255)
                                                                                                           ttccGAGCGCGTTGATAGCATTTGTAGGCCTGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGAtt                                           <  1:309344/148‑1 (MQ=255)
                                                                                                           gACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGAtt                                           >  2:578698/1‑150 (MQ=255)
                                                                                                            aCCGTGCGCGTTGATTGCATTTGTAGGCCGGATAAGGCTTTCACGCCGCATCCGGAACTCTTTCAGCAACATGCTTTGCGGAGTCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAATTATTTAGGTCAGGCGGCGAAGCTGATGATTg                                          <  1:114066/150‑1 (MQ=255)
                                                                                                            aCCGAGCGCTTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTg                                          <  2:1837979/150‑1 (MQ=255)
                                                                                                             ccGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTgg                                         <  2:2003762/150‑1 (MQ=255)
                                                                                                              cGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                        >  1:1445754/1‑150 (MQ=255)
                                                                                                              cGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                        >  1:269095/1‑150 (MQ=255)
                                                                                                                  cgcgTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGc                                    >  1:224624/1‑150 (MQ=255)
                                                                                                                  cgcgTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGc                                    >  2:1327976/1‑150 (MQ=255)
                                                                                                                    cgTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCt                                  >  1:191686/1‑150 (MQ=255)
                                                                                                                    cgTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCt                                  >  1:826614/1‑150 (MQ=255)
                                                                                                                     gTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTg                                 >  1:185041/1‑150 (MQ=255)
                                                                                                                     gTTGATAGCATTTGTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCACTCTTTCAGCCACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTg                                 <  1:1531288/150‑1 (MQ=255)
                                                                                                                      ttGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                >  1:1243989/1‑150 (MQ=255)
                                                                                                                       tGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                               >  2:1309372/1‑150 (MQ=255)
                                                                                                                        gATAGCATTTGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAtt                              <  2:1208302/150‑1 (MQ=255)
                                                                                                                          tAGCATTTGTTGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGTCCTAGATGTTTGATTCACTGTCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                            <  1:1426290/150‑1 (MQ=255)
                                                                                                                          tAGCAGTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTTGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                            <  2:1821405/150‑1 (MQ=255)
                                                                                                                           aGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACg                           <  2:200828/150‑1 (MQ=255)
                                                                                                                             cATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATGCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGAc                         >  1:833382/1‑150 (MQ=255)
                                                                                                                             cATTTGTAGGCAGGATAAGGAGTTCACGCCGCATCCGGCACTGTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAACCAGGAAAATTTTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTTATTACGAc                         <  2:565532/150‑1 (MQ=255)
                                                                                                                                  gagtgCCGGATGCGGCGTGAACGCCTTATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTa                    >  2:717018/5‑150 (MQ=255)
                                                                                                                                    ttgCCGGATGCGGCGTGAACGCCTTATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGAAAACTATg                  >  1:189419/3‑150 (MQ=255)
                                                                                                                                    aGGCCGGATAAGGTGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATg                  >  2:1345851/1‑150 (MQ=255)
                                                                                                                                    aGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATg                  >  2:1994975/1‑150 (MQ=255)
                                                                                                                                     ggCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGt                 >  2:1558443/1‑150 (MQ=255)
                                                                                                                                      gCCGGATGCGGCGTGAACGCCTTATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                >  2:1971551/1‑150 (MQ=255)
                                                                                                                                      gCCGGATGCGGCGTGAACGCCTTATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                >  1:1272905/1‑150 (MQ=255)
                                                                                                                                      gCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                <  1:1062220/150‑1 (MQ=255)
                                                                                                                                      gCCGGATAAGGCGTGCACTCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCg               <  2:1216615/150‑1 (MQ=255)
                                                                                                                                       ccGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCg               >  2:272605/1‑150 (MQ=255)
                                                                                                                                       ccGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGAATCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACAATGTCg               >  2:1027577/1‑150 (MQ=255)
                                                                                                                                           aTACGGCGTTCACGCCGCTTCCGGCACTCTTTCAGCAACATGGATAGCGGAGGCCAAGATGTTTGATTCACTGTCAAAAGGCGGAAAATATTTAGGTCAGGCGTCGAAGCTGATGATTTGTATGCCTGATTACGACAACTATGTCGAACa           <  1:984396/150‑1 (MQ=255)
                                                                                                                                             aaGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata         >  1:73816/1‑150 (MQ=255)
                                                                                                                                              aGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACatat        >  1:604694/1‑150 (MQ=255)
                                                                                                                                                 cGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTCTGCCTGATTACGACAACTATGTCGAACATATGCg     >  1:1367526/1‑150 (MQ=255)
                                                                                                                                                 cGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg     >  1:587831/1‑150 (MQ=255)
                                                                                                                                                  gTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg    >  1:365142/1‑150 (MQ=255)
                                                                                                                                                  gTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg    >  2:1860631/1‑150 (MQ=255)
                                                                                                                                                   ttCACGCCGCATCAGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg   <  2:1817462/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGT  >  NC_000913/631985‑632282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: