Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 632,140 G→T 7.8% intergenic (+141/‑42) cstA → / → ybdD carbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913632,1400GT 7.8% 326.6 / 12.3 116intergenic (+141/‑42)cstA/ybdDcarbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein
Reads supporting (aligned to +/- strand):  ref base G (59/46);  new base T (4/5);  total (65/51)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 122 total aligned reads displayed.

GCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACC  >  NC_000913/631991‑632287
                                                                                                                                                     |                                                                                                                                                   
gcACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcgccg                                                                                                                                                     >  2:1062220/1‑150 (MQ=255)
gcACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcgccg                                                                                                                                                     <  1:1423733/150‑1 (MQ=255)
     cTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                                                                >  2:690750/1‑150 (MQ=255)
     cTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGATCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                <  1:1380614/150‑1 (MQ=38)
      aaaaGGCAGCGGGCGGGGGGGATGTTGGCGAATGCTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                                               <  1:1994376/149‑1 (MQ=39)
        acgTCAGAGGGAGGGTGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTTATAGCATTTGTAGGCGGGATAAGTCGTTCACGCCGCATCCGGc                                                                                                                                             <  2:1246037/148‑1 (MQ=39)
        aaGTCAGCGGGCGGGGGGGATGTTGGCGACTGTTGGCGTAGGGCCCAGGGGTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                             <  1:369583/150‑1 (MQ=37)
        aaGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                             <  2:1178282/150‑1 (MQ=39)
            cAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGGGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGcctac                                                                                                                                         <  1:1481474/150‑5 (MQ=38)
                 ggAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCa                                                                                                                                    <  2:75109/149‑1 (MQ=255)
                   aGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGc                                                                                                                                  <  2:1127846/150‑1 (MQ=255)
                          gATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATgg                                                                                                                           <  2:939536/150‑1 (MQ=255)
                            tGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATACGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGtt                                                                                                                         >  1:1237475/1‑150 (MQ=255)
                            tGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGtt                                                                                                                         >  1:1912064/1‑150 (MQ=255)
                               tGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGc                                                                                                                      >  2:1304997/1‑150 (MQ=255)
                                 gCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCgg                                                                                                                    <  1:1109919/150‑1 (MQ=255)
                                       gTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGTACTCTTTCAGCAACATGGTTAGCGGAGGCCa                                                                                                              <  1:891237/150‑1 (MQ=255)
                                          ggCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCACATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGa                                                                                                           >  1:654669/1‑150 (MQ=255)
                                             ttAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGt                                                                                                        <  1:1650464/150‑1 (MQ=255)
                                              tAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGtt                                                                                                       <  2:754976/150‑1 (MQ=255)
                                              tAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGtt                                                                                                       <  2:1461315/150‑1 (MQ=255)
                                              tAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACGCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGtt                                                                                                       >  1:1681726/1‑150 (MQ=255)
                                              tAGGGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGTATAAGGCGTTCACGCCGCATCAGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGtt                                                                                                       <  1:1227075/150‑1 (MQ=255)
                                                  gCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGAt                                                                                                   <  1:1543842/150‑1 (MQ=255)
                                                   cccAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGAtt                                                                                                  <  1:1462999/150‑1 (MQ=255)
                                                   cccAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGAtt                                                                                                  >  2:1611930/1‑150 (MQ=255)
                                                    ccAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTc                                                                                                 <  2:1390906/150‑1 (MQ=255)
                                                       gggTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATATCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACt                                                                                              <  1:1025594/150‑1 (MQ=255)
                                                       gggTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACt                                                                                              >  1:1642187/1‑150 (MQ=255)
                                                          ttCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTTACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGc                                                                                           <  1:689665/150‑1 (MQ=255)
                                                            cccTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCaa                                                                                         >  2:921490/1‑150 (MQ=255)
                                                            cccTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGAGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCaa                                                                                         >  2:1604650/1‑150 (MQ=255)
                                                             ccTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCaaa                                                                                        >  1:495162/1‑150 (MQ=255)
                                                              ctctCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCaaaa                                                                                       >  2:350881/1‑150 (MQ=255)
                                                                  cACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCATCATGGTTAGCGGAGGCCAAGATGTTTGATTCTCTGGCAAAAGCCg                                                                                   >  1:1576046/1‑150 (MQ=255)
                                                                  cACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCg                                                                                   >  2:863155/1‑150 (MQ=255)
                                                                   aCCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCgg                                                                                  <  1:464092/150‑1 (MQ=255)
                                                                   aCCCTAACCCTCTCCCCGGGGGGGCGAGGGGACTGCCCGCGCGCGTTGATAGCATGTGTAGGCCGGATAAGGAGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCgg                                                                                  <  1:299333/150‑1 (MQ=255)
                                                                    cccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGa                                                                                 >  2:173564/1‑150 (MQ=255)
                                                                    cccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGa                                                                                 >  1:1770875/1‑150 (MQ=255)
                                                                    cccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGa                                                                                 >  1:560834/1‑150 (MQ=255)
                                                                       tAACCCTCTCCCCGGTGGGGCGAGGGGACTGCCCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCATGATGTTTGATTCACTGGCAAAAGCCGGaaaa                                                                              >  1:1877996/1‑150 (MQ=255)
                                                                       tAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGaaaa                                                                              >  1:1109175/1‑150 (MQ=255)
                                                                       gtccccTCGCCCCACCGGGGAGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGaaaa                                                                              >  1:1583709/4‑150 (MQ=39)
                                                                         ccccTCGCCCCACCGGGGAGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGCCAAAAGCCCGAAAata                                                                            >  2:1992027/2‑150 (MQ=38)
                                                                          cccTCGCCCCACCGGGGAGAGGGGACTGACCGAGCGCGTTGCTAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCCCTCTTTCAGCAACATGGGTAGCGGAGGCCAAGATGTTTGAGTCACTGGCAAACGCCGGAAAattt                                                                           >  1:1224136/1‑148 (MQ=38)
                                                                           ccTCTCCCCGGTGGGGCGAAGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACAGGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATAtt                                                                          >  2:919712/1‑150 (MQ=255)
                                                                              ctcCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAg                                                                       >  1:245608/1‑150 (MQ=255)
                                                                                ccccGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACCGGCAAAAGCCGGAAAATATTTAGGt                                                                     >  2:934375/1‑150 (MQ=255)
                                                                                 cccGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTc                                                                    >  1:1966186/1‑150 (MQ=255)
                                                                                        gggCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCTCTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcggc                                                             >  1:142458/1‑150 (MQ=255)
                                                                                        gggCGAGGGGACTGACCGAGCGCGGTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggcggc                                                             <  2:767434/150‑1 (MQ=255)
                                                                                         ggCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATACGGCGGTCACGCCGCATCCGGCACTCTTTCAGCAACATGGGTAGCGGAGGCCAAGATGTTTGATTCTCTGGCAAAAGCCGGAAAATAGTTAGGTCAGgcggcg                                                            >  1:307929/1‑150 (MQ=255)
                                                                                         ggCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGgcggcg                                                            >  1:220890/1‑150 (MQ=255)
                                                                                                 gACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgat                                                    >  2:501686/1‑150 (MQ=255)
                                                                                                 gACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgat                                                    >  2:1405925/1‑150 (MQ=255)
                                                                                                   cTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                  >  1:611960/1‑150 (MQ=255)
                                                                                                   cTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCtgatga                                                  >  1:1489958/1‑150 (MQ=255)
                                                                                                     ttccGAGCGCGTTGATAGCATTTGTAGGCCTGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGAtt                                                <  1:309344/148‑1 (MQ=255)
                                                                                                     gACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGAtt                                                >  2:578698/1‑150 (MQ=255)
                                                                                                      cccGAGCTCGTTGATATCATTTGTAGGCCGGATAAGGAGTTCACGCCTCATCCTGCACTCTTTCAGCAACATGGTTAGCGGAGGCCATGATGTTTTATTCACTGGCAAAAGCCGGAAAATATATAGGTCAGGCGGCGAAGCTGATGATTg                                               <  2:448587/149‑1 (MQ=255)
                                                                                                      aCCGTGCGCGTTGATTGCATTTGTAGGCCGGATAAGGCTTTCACGCCGCATCCGGAACTCTTTCAGCAACATGCTTTGCGGAGTCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAATTATTTAGGTCAGGCGGCGAAGCTGATGATTg                                               <  1:114066/150‑1 (MQ=255)
                                                                                                      aCCGAGCGCTTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTg                                               <  2:1837979/150‑1 (MQ=255)
                                                                                                        cGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                             >  1:269095/1‑150 (MQ=255)
                                                                                                        cGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGt                                             >  1:1445754/1‑150 (MQ=255)
                                                                                                            cgcgTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGc                                         >  2:1327976/1‑150 (MQ=255)
                                                                                                            cgcgTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGc                                         >  1:224624/1‑150 (MQ=255)
                                                                                                               gTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTg                                      >  1:185041/1‑150 (MQ=255)
                                                                                                               gTTGATAGCATTTGTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCACTCTTTCAGCCACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTg                                      <  1:1531288/150‑1 (MQ=255)
                                                                                                                ttGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGa                                     >  1:1243989/1‑150 (MQ=255)
                                                                                                                  gATAGCATTTGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAtt                                   <  2:1208302/150‑1 (MQ=255)
                                                                                                                    tAGCATTTGTTGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGTCCTAGATGTTTGATTCACTGTCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                 <  1:1426290/150‑1 (MQ=255)
                                                                                                                    tAGCAGTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTTGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                 <  2:1821405/150‑1 (MQ=255)
                                                                                                                     aGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACg                                <  2:200828/150‑1 (MQ=255)
                                                                                                                       cATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATGCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGAc                              >  1:833382/1‑150 (MQ=255)
                                                                                                                       cATTTGTAGGCAGGATAAGGAGTTCACGCCGCATCCGGCACTGTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAACCAGGAAAATTTTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTTATTACGAc                              <  2:565532/150‑1 (MQ=255)
                                                                                                                            gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTa                         <  2:1839461/150‑1 (MQ=255)
                                                                                                                              ttgCCGGATGCGGCGTGAACGCCTTATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGAAAACTATg                       >  1:189419/3‑150 (MQ=255)
                                                                                                                              aGGCCGGATAAGGTGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATg                       >  2:1345851/1‑150 (MQ=255)
                                                                                                                              aGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATg                       >  2:1994975/1‑150 (MQ=255)
                                                                                                                               ggCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGt                      >  2:1558443/1‑150 (MQ=255)
                                                                                                                                gCCGGATGCGGCGTGAACGCCTTATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                     >  1:1272905/1‑150 (MQ=255)
                                                                                                                                gCCGGATGCGGCGTGAACGCCTTATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                     >  2:1971551/1‑150 (MQ=255)
                                                                                                                                gCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                     <  1:1062220/150‑1 (MQ=255)
                                                                                                                                gCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTc                     >  1:1755381/1‑150 (MQ=255)
                                                                                                                                gCCGGATAAGGCGTGCACTCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCg                    <  2:1216615/150‑1 (MQ=255)
                                                                                                                                 ccGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCg                    <  2:1154324/150‑1 (MQ=255)
                                                                                                                                 ccGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCg                    >  2:272605/1‑150 (MQ=255)
                                                                                                                                 ccGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGAATCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACAATGTCg                    >  2:1027577/1‑150 (MQ=255)
                                                                                                                                     aTACGGCGTTCACGCCGCTTCCGGCACTCTTTCAGCAACATGGATAGCGGAGGCCAAGATGTTTGATTCACTGTCAAAAGGCGGAAAATATTTAGGTCAGGCGTCGAAGCTGATGATTTGTATGCCTGATTACGACAACTATGTCGAACa                <  1:984396/150‑1 (MQ=255)
                                                                                                                                       aaGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata              >  1:73816/1‑150 (MQ=255)
                                                                                                                                           cGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTCTGCCTGATTACGACAACTATGTCGAACATATGCg          >  1:1367526/1‑150 (MQ=255)
                                                                                                                                           cGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg          >  1:587831/1‑150 (MQ=255)
                                                                                                                                            gTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg         >  2:1860631/1‑150 (MQ=255)
                                                                                                                                             ttCACGCCGCATCAGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg        <  2:1817462/150‑1 (MQ=255)
                                                                                                                                              tCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt       <  2:864647/150‑1 (MQ=255)
                                                                                                                                              tCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt       >  2:1994732/1‑150 (MQ=255)
                                                                                                                                                aCGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAATCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa     <  2:471277/150‑1 (MQ=255)
                                                                                                                                                 cgccgcATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGACGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa    >  2:1195289/1‑150 (MQ=255)
                                                                                                                                                   ccgcATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc  <  2:1237475/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACC  >  NC_000913/631991‑632287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: