Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 729,376 G→T 6.6% intergenic (+36/‑207) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913729,3760GT 6.6% 397.9 / 12.7 142intergenic (+36/‑207)ybfA/rhsCDUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base G (70/62);  new base T (7/3);  total (77/65)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 143 total aligned reads displayed.

TTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTAT  >  NC_000913/729227‑729523
                                                                                                                                                     |                                                                                                                                                     
    ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAAc                                                                                                                                                   >  2:1361092/1‑150 (MQ=255)
    ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAAc                                                                                                                                                   >  1:885122/1‑150 (MQ=255)
         gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGATTACCAGCGATTAACCGGTGTGGATGGTTCAGGCCGAAAAGGCAACTGGTGATTTTTTTTGATTGGTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                              <  1:348704/150‑1 (MQ=255)
           ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                            <  1:477171/150‑1 (MQ=255)
            ctcgcCCGCTTTTACAGCTACCGGCATCGCGTCTGGGCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCgg                                                                                                                                           <  1:1393774/148‑1 (MQ=255)
             gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGt                                                                                                                                          >  2:1892803/1‑150 (MQ=255)
              cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtt                                                                                                                                         <  1:1929410/150‑1 (MQ=255)
              cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtt                                                                                                                                         >  1:1771418/1‑150 (MQ=255)
               gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGGGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGttt                                                                                                                                        <  2:1548486/150‑1 (MQ=255)
                cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGtttt                                                                                                                                       <  2:737179/150‑1 (MQ=255)
                cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCCCCAGCCGAAACGCTGGCCGtttt                                                                                                                                       >  2:1663755/1‑150 (MQ=255)
                   gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTg                                                                                                                                  <  2:1197408/150‑1 (MQ=255)
                          cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAgg                                                                                                                           >  1:1341248/1‑150 (MQ=255)
                          cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAgg                                                                                                                           >  1:537855/1‑150 (MQ=255)
                          cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGGTTT‑‑TTGTATCAgg                                                                                                                           >  1:1799437/1‑150 (MQ=255)
                          cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATGTTTATTGATTGCGGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAgg                                                                                                                           >  1:317363/1‑150 (MQ=255)
                          cAGCTACCTGCATCGCGGCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGAGCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAgg                                                                                                                           <  1:1312852/150‑1 (MQ=255)
                           aGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGa                                                                                                                          <  2:1064723/150‑1 (MQ=255)
                            gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGaa                                                                                                                         >  1:963511/1‑150 (MQ=255)
                            gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGaa                                                                                                                         >  2:1104568/1‑150 (MQ=255)
                               aCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGAt                                                                                                                      >  1:1187835/1‑150 (MQ=255)
                                ccTGCATCGCGTCTGTTCGAAAACCAGCGATAAACCGGTGTGTATGTATCAGGCGTAAAAGTCAACTGGGGATTTTAATTGATTGATGATTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATg                                                                                                                     <  2:403320/150‑1 (MQ=255)
                                ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATg                                                                                                                     <  2:1417953/150‑1 (MQ=255)
                                 cagCATCGCGGCTTGTCGAAACCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGt                                                                                                                    <  2:82685/148‑1 (MQ=255)
                                 cTGCATCGCGTCTGTTCGAAATCCAGCGATAAACCTGTGTGGATGGATCAGGCCGATAAGGCAACTGGTGATTTTTATTGATTGCTGTGTTTTGAATACATTGAAAACCGCCAGCGGCAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGt                                                                                                                    <  1:312717/150‑1 (MQ=255)
                                  tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGtt                                                                                                                   >  1:987616/1‑150 (MQ=255)
                                  tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGtt                                                                                                                   >  2:1097682/1‑150 (MQ=255)
                                   gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTc                                                                                                                  <  2:1229134/150‑1 (MQ=255)
                                   gCATCGCGTCTGGGCGAAAACCAGCGATAAACCGGTGTGGATGTATCAGGCCGAAAAGGCAACTTGGTATTTTTATTGATTGCTGTGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTc                                                                                                                  <  1:1482489/150‑1 (MQ=255)
                                     aTCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATGTTTATAGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCag                                                                                                                <  1:505966/150‑1 (MQ=255)
                                        gcgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAgg                                                                                                             >  2:254012/1‑150 (MQ=255)
                                         cgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCCCCAGCGGAAACGCTGGCGGTTTT‑‑TTTTATCAGGAAGATGTTCAGAGGa                                                                                                            >  1:1270770/1‑150 (MQ=255)
                                             tGGTCGAAAACCAGCGTTAAACGGGTGTTGATGGTTCAGGCCGAAAAGGCAACTGGTGATTTTAATTGATTGTTGAGTTTTGAATACATTGAAAACCTCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAatat                                                                                                        <  2:406808/150‑1 (MQ=255)
                                              ggTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATAtt                                                                                                       >  1:670557/1‑150 (MQ=255)
                                               gTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                                                      >  2:68159/1‑150 (MQ=255)
                                               gTCGAAAACCAGCGATAAACCGGTGTGAATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                                                      >  2:1142119/1‑150 (MQ=255)
                                                tCGAAAACCAGCGATAAACCGGTGTGGATGGATCCGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCTCCAGCGGAAACGCTTGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                     >  2:806403/1‑150 (MQ=255)
                                                tCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                     >  2:1298057/1‑150 (MQ=255)
                                                 cGAAAACCAGCTATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                                                    <  2:525624/150‑1 (MQ=255)
                                                  gAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGa                                                                                                   >  2:1375656/1‑150 (MQ=255)
                                                      aCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATc                                                                                               >  1:1839449/1‑150 (MQ=255)
                                                        cAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCGGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                                             <  2:1025035/150‑1 (MQ=255)
                                                         aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                                            >  2:430424/1‑150 (MQ=255)
                                                         aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                                            >  2:2014482/1‑150 (MQ=255)
                                                         aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                                            >  2:1667807/1‑150 (MQ=255)
                                                          gCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTta                                                                                           <  2:1708339/150‑1 (MQ=255)
                                                                 aCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAAt                                                                                    <  1:1813281/150‑1 (MQ=255)
                                                                   cGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTAATTGATTGCTGAGTTTTGAAAACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCg                                                                                  <  2:1016812/150‑1 (MQ=255)
                                                                    ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                                                 >  1:1295583/1‑150 (MQ=255)
                                                                         ggatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACat                                                                            >  2:468387/1‑150 (MQ=255)
                                                                         ggatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACat                                                                            >  1:1014297/1‑150 (MQ=255)
                                                                         ggatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACat                                                                            >  1:1359006/1‑150 (MQ=255)
                                                                          gatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACata                                                                           >  1:950118/1‑150 (MQ=255)
                                                                           atggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatat                                                                          >  2:1177423/1‑150 (MQ=255)
                                                                                tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                                     <  1:1024439/150‑1 (MQ=255)
                                                                                tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                                     <  1:1358694/150‑1 (MQ=255)
                                                                                  aGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATAAATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATg                                                                   <  1:377517/150‑1 (MQ=255)
                                                                                    gCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaa                                                                 >  1:210887/1‑150 (MQ=255)
                                                                                     ccGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTGTT‑‑TTGTATCAGGAAGATGTTCAGAGGATTATTTAGACATCGTTATACCAATCGAAACATATATTCATGaaa                                                                >  2:337234/1‑150 (MQ=255)
                                                                                             gCAACTGTTGATTTTTATTTATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAAtatatata                                                        <  2:881776/150‑1 (MQ=255)
                                                                                               aaCTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATaaa                                                      <  2:1113263/150‑1 (MQ=255)
                                                                                                aCTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAat                                                     <  2:1560159/150‑1 (MQ=255)
                                                                                                     tGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtttt                                                >  2:673608/1‑150 (MQ=255)
                                                                                                      taTTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTc                                               <  1:1603519/149‑1 (MQ=255)
                                                                                                      gATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGTATATTTAGACATCGTTTTACCAATCGAAACATATATTCATGAAATATATATAAATAtttta                                               >  2:1465012/1‑149 (MQ=255)
                                                                                                       tttttttttGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTcc                                              <  2:1270770/149‑1 (MQ=255)
                                                                                                       aTTTTTATTGATTGCTGAGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTcc                                              <  1:1004700/150‑1 (MQ=255)
                                                                                                                tattgCTGAGTTTTGAATACATTGAAAACCGCCATCGGAAACGCTGGCTGTTTT‑‑TTGTATCAGGAAGATGTTCAGATGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTc                                     <  2:1234431/149‑1 (MQ=255)
                                                                                                                tattgATGAGTTTTGAATACATTGAAAACCGGCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCGTAATTGTTc                                     <  2:1433401/149‑1 (MQ=255)
                                                                                                                 attgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                    <  2:1839449/150‑1 (MQ=255)
                                                                                                                    gCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCCGGAAGATGTTCAGAGGAATATTTAGACATCGTGATACGAATCGATACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTAt                                 >  2:326558/1‑150 (MQ=255)
                                                                                                                     cTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATc                                <  2:1400438/150‑1 (MQ=255)
                                                                                                                     cTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATc                                >  1:1108448/1‑150 (MQ=255)
                                                                                                                       gAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTg                              >  2:732392/1‑150 (MQ=255)
                                                                                                                         gTTTTGAATACATTGAAAACCGCCAGAGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGAc                            <  2:944963/150‑1 (MQ=255)
                                                                                                                           ttttAATACATTTAAAACCGCCAGCGGAAACTCTTGCGGTTTT‑‑TTGTATCATGAAGATGTTCATAGTAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg                          <  1:1523493/150‑1 (MQ=255)
                                                                                                                           tttGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg                          >  2:266883/1‑150 (MQ=255)
                                                                                                                            ttGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATAAATAAATATTTTCCTAATTGTTCTTATCTGACAGa                         <  2:1960143/150‑1 (MQ=255)
                                                                                                                              taaTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGata                       <  1:251043/149‑1 (MQ=255)
                                                                                                                                aTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                     >  2:551710/1‑150 (MQ=255)
                                                                                                                                aTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAATCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                     >  2:90643/1‑150 (MQ=255)
                                                                                                                                 tACATTGAAAACCGCCAGCGTAAACGCTGGCGGTTTT‑‑TTTTATCAGGAAGATTTTCAGAGGAATATTTATAAAGCGGTATAGCAATCGAAACATATATTCATGAAATTTATATAAATATTTTCCTAATTGTTATTTTCTGACAGatatat                    >  2:284336/1‑148 (MQ=255)
                                                                                                                                  aCATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtctc                   <  1:1814666/150‑1 (MQ=255)
                                                                                                                                   cATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTGTTATCTGACAGATAtctaa                  >  1:155684/1‑148 (MQ=255)
                                                                                                                                    aTTGAAAACCGCCCGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCGCAc                 >  1:1393294/1‑150 (MQ=255)
                                                                                                                                      aaaaaaCCGCCAGCGTTTCCGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATATGACAGATATCTCACtt               >  1:2010682/3‑150 (MQ=255)
                                                                                                                                       gAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGATACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGGTTTCTCTCttt              >  2:762231/1‑149 (MQ=255)
                                                                                                                                       gAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTa              >  1:312996/1‑150 (MQ=255)
                                                                                                                                         aaaCCGCCAGCGTTTCCGCTGGCGGTTTTCAATGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATATCAGTTa              >  1:1943192/1‑150 (MQ=38)
                                                                                                                                         aaaCCGCCAGCGTTTCCGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTAACTTAAg            >  1:1327288/1‑150 (MQ=255)
                                                                                                                                         aaaCCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATTAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg            <  2:1290634/150‑1 (MQ=255)
                                                                                                                                         aaaCCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg            >  1:1636714/1‑150 (MQ=255)
                                                                                                                                         aaaCCGCAAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg            <  1:835218/150‑1 (MQ=255)
                                                                                                                                           aCCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAgga          >  1:371937/1‑149 (MQ=255)
                                                                                                                                            ccGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCt         >  2:1443542/1‑150 (MQ=255)
                                                                                                                                            ccGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCt         >  1:608285/1‑150 (MQ=255)
                                                                                                                                            ccGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCt         >  2:452762/1‑150 (MQ=255)
                                                                                                                                                cAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCt     >  2:1053913/1‑150 (MQ=255)
                                                                                                                                                  gCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTta   >  2:858478/1‑150 (MQ=255)
                                                                                                                                                  gCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTta   <  2:1187835/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTAT  >  NC_000913/729227‑729523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: