Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 729,377 A→T 6.4% intergenic (+37/‑206) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913729,3770AT 6.4% 403.4 / 14.0 140intergenic (+37/‑206)ybfA/rhsCDUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (69/61);  new base T (6/3);  total (75/65)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

Only 100 of 143 total aligned reads displayed.

TCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAA  >  NC_000913/729228‑729526
                                                                                                                                                     |                                                                                                                                                       
   ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAAc                                                                                                                                                      >  1:885122/1‑150 (MQ=255)
   ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAAc                                                                                                                                                      >  2:1361092/1‑150 (MQ=255)
        gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGATTACCAGCGATTAACCGGTGTGGATGGTTCAGGCCGAAAAGGCAACTGGTGATTTTTTTTGATTGGTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                                 <  1:348704/150‑1 (MQ=255)
          ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                               <  1:477171/150‑1 (MQ=255)
           ctcgcCCGCTTTTACAGCTACCGGCATCGCGTCTGGGCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCgg                                                                                                                                              <  1:1393774/148‑1 (MQ=255)
            gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGt                                                                                                                                             >  2:1892803/1‑150 (MQ=255)
             cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtt                                                                                                                                            <  1:1929410/150‑1 (MQ=255)
             cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtt                                                                                                                                            >  1:1771418/1‑150 (MQ=255)
              gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGGGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGttt                                                                                                                                           <  2:1548486/150‑1 (MQ=255)
               cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGtttt                                                                                                                                          <  2:737179/150‑1 (MQ=255)
               cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCCCCAGCCGAAACGCTGGCCGtttt                                                                                                                                          >  2:1663755/1‑150 (MQ=255)
                  gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTg                                                                                                                                     <  2:1197408/150‑1 (MQ=255)
                   cTTTTACAGCTACCTGCCTCGCGTCTGGTCGAATACCAGCGATTAGCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGt                                                                                                                                    >  2:302179/1‑150 (MQ=255)
                         cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAgg                                                                                                                              >  1:537855/1‑150 (MQ=255)
                         cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAgg                                                                                                                              >  1:1341248/1‑150 (MQ=255)
                         cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGGTTT‑‑TTGTATCAgg                                                                                                                              >  1:1799437/1‑150 (MQ=255)
                         cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATGTTTATTGATTGCGGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAgg                                                                                                                              >  1:317363/1‑150 (MQ=255)
                         cAGCTACCTGCATCGCGGCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGAGCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAgg                                                                                                                              <  1:1312852/150‑1 (MQ=255)
                          aGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGa                                                                                                                             <  2:1064723/150‑1 (MQ=255)
                           gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCATCGGAAACGCTTGCGGTTTT‑‑TTGTATCAGGaa                                                                                                                            >  2:377517/1‑150 (MQ=255)
                           gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGaa                                                                                                                            >  1:963511/1‑150 (MQ=255)
                           gCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGaa                                                                                                                            >  2:1104568/1‑150 (MQ=255)
                              aCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGAt                                                                                                                         >  1:1187835/1‑150 (MQ=255)
                               ccTGCATCGCGTCTGTTCGAAAACCAGCGATAAACCGGTGTGTATGTATCAGGCGTAAAAGTCAACTGGGGATTTTAATTGATTGATGATTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATg                                                                                                                        <  2:403320/150‑1 (MQ=255)
                               ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATg                                                                                                                        <  2:1417953/150‑1 (MQ=255)
                                cagCATCGCGGCTTGTCGAAACCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGt                                                                                                                       <  2:82685/148‑1 (MQ=255)
                                cTGCATCGCGTCTGTTCGAAATCCAGCGATAAACCTGTGTGGATGGATCAGGCCGATAAGGCAACTGGTGATTTTTATTGATTGCTGTGTTTTGAATACATTGAAAACCGCCAGCGGCAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGt                                                                                                                       <  1:312717/150‑1 (MQ=255)
                                 tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGtt                                                                                                                      >  2:1097682/1‑150 (MQ=255)
                                 tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGtt                                                                                                                      >  1:987616/1‑150 (MQ=255)
                                  gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTc                                                                                                                     <  2:1229134/150‑1 (MQ=255)
                                  gCATCGCGTCTGGGCGAAAACCAGCGATAAACCGGTGTGGATGTATCAGGCCGAAAAGGCAACTTGGTATTTTTATTGATTGCTGTGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTc                                                                                                                     <  1:1482489/150‑1 (MQ=255)
                                    aTCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATGTTTATAGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCag                                                                                                                   <  1:505966/150‑1 (MQ=255)
                                       gcgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAgg                                                                                                                >  2:254012/1‑150 (MQ=255)
                                        cgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCCCCAGCGGAAACGCTGGCGGTTTT‑‑TTTTATCAGGAAGATGTTCAGAGGa                                                                                                               >  1:1270770/1‑150 (MQ=255)
                                             ggTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATAtt                                                                                                          >  1:670557/1‑150 (MQ=255)
                                              gTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                                                         >  2:68159/1‑150 (MQ=255)
                                              gTCGAAAACCAGCGATAAACCGGTGTGAATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                                                         >  2:1142119/1‑150 (MQ=255)
                                               tCGAAAACCAGCGATAAACCGGTGTGGATGGATCCGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCTCCAGCGGAAACGCTTGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                        >  2:806403/1‑150 (MQ=255)
                                               tCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                        >  2:1298057/1‑150 (MQ=255)
                                                cGAAAACCAGCTATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                                                       <  2:525624/150‑1 (MQ=255)
                                                 gAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGa                                                                                                      >  2:1375656/1‑150 (MQ=255)
                                                     aCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATc                                                                                                  >  1:1839449/1‑150 (MQ=255)
                                                       cAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCGGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                                                <  2:1025035/150‑1 (MQ=255)
                                                       cAGCGATAAACCGGGGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                                                <  2:427064/150‑1 (MQ=255)
                                                        aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                                               >  2:430424/1‑150 (MQ=255)
                                                        aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                                               >  2:1667807/1‑150 (MQ=255)
                                                         gCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTta                                                                                              <  2:1708339/150‑1 (MQ=255)
                                                                aCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAAt                                                                                       <  1:1813281/150‑1 (MQ=255)
                                                                  cGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTAATTGATTGCTGAGTTTTGAAAACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCg                                                                                     <  2:1016812/150‑1 (MQ=255)
                                                                   ggTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGa                                                                                    >  1:1295583/1‑150 (MQ=255)
                                                                        ggatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACat                                                                               >  1:1014297/1‑150 (MQ=255)
                                                                        ggatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACat                                                                               >  1:1359006/1‑150 (MQ=255)
                                                                         gatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACata                                                                              >  1:950118/1‑150 (MQ=255)
                                                                          atggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatat                                                                             >  2:1177423/1‑150 (MQ=255)
                                                                               tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                                        <  1:1358694/150‑1 (MQ=255)
                                                                               tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCa                                                                        <  1:1024439/150‑1 (MQ=255)
                                                                                 aGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATAAATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATg                                                                      <  1:377517/150‑1 (MQ=255)
                                                                                   gCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaa                                                                    >  1:210887/1‑150 (MQ=255)
                                                                                            gCAACTGTTGATTTTTATTTATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAAtatatata                                                           <  2:881776/150‑1 (MQ=255)
                                                                                              aaCTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATaaa                                                         <  2:1113263/150‑1 (MQ=255)
                                                                                               aCTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAat                                                        <  2:1560159/150‑1 (MQ=255)
                                                                                                    tGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtttt                                                   >  2:673608/1‑150 (MQ=255)
                                                                                                     taTTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTc                                                  <  1:1603519/149‑1 (MQ=255)
                                                                                                     gATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCCGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGTATATTTAGACATCGTTTTACCAATCGAAACATATATTCATGAAATATATATAAATAtttta                                                  >  2:1465012/1‑149 (MQ=255)
                                                                                                      tttttttttGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTcc                                                 <  2:1270770/149‑1 (MQ=255)
                                                                                                      aTTTTTATTGATTGCTGAGTTTTTAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTcc                                                 <  1:1004700/150‑1 (MQ=255)
                                                                                                               tattgCTGAGTTTTGAATACATTGAAAACCGCCATCGGAAACGCTGGCTGTTTT‑‑TTGTATCAGGAAGATGTTCAGATGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTc                                        <  2:1234431/149‑1 (MQ=255)
                                                                                                               tattgATGAGTTTTGAATACATTGAAAACCGGCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCGTAATTGTTc                                        <  2:1433401/149‑1 (MQ=255)
                                                                                                                attgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCt                                       <  2:1839449/150‑1 (MQ=255)
                                                                                                                   gCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCCGGAAGATGTTCAGAGGAATATTTAGACATCGTGATACGAATCGATACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTAt                                    >  2:326558/1‑150 (MQ=255)
                                                                                                                    cTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATc                                   <  2:1400438/150‑1 (MQ=255)
                                                                                                                    cTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATc                                   >  1:1108448/1‑150 (MQ=255)
                                                                                                                      gAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTg                                 >  2:732392/1‑150 (MQ=255)
                                                                                                                        gTTTTGAATACATTGAAAACCGCCAGAGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGAc                               <  2:944963/150‑1 (MQ=255)
                                                                                                                          ttttAATACATTTAAAACCGCCAGCGGAAACTCTTGCGGTTTT‑‑TTGTATCATGAAGATGTTCATAGTAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg                             <  1:1523493/150‑1 (MQ=255)
                                                                                                                          tttGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg                             >  2:266883/1‑150 (MQ=255)
                                                                                                                           ttGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATAAATAAATATTTTCCTAATTGTTCTTATCTGACAGa                            <  2:1960143/150‑1 (MQ=255)
                                                                                                                             taaTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGata                          <  1:251043/149‑1 (MQ=255)
                                                                                                                               aTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                        >  2:551710/1‑150 (MQ=255)
                                                                                                                               aTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAATCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                        >  2:90643/1‑150 (MQ=255)
                                                                                                                                 aCATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtctc                      <  1:1814666/150‑1 (MQ=255)
                                                                                                                                  cATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTGTTATCTGACAGATAtctaa                     >  1:155684/1‑148 (MQ=255)
                                                                                                                                   aTTGAAAACCGCCCGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCGCAc                    >  1:1393294/1‑150 (MQ=255)
                                                                                                                                    caaaaaaCCGCCAGCGTTTCCGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACt                   >  2:1948338/4‑150 (MQ=255)
                                                                                                                                     tGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACtt                  <  2:210887/150‑1 (MQ=255)
                                                                                                                                     aaaaaaCCGCCAGCGTTTCCGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATATGACAGATATCTCACtt                  >  1:2010682/3‑150 (MQ=255)
                                                                                                                                      gAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGATACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGGTTTCTCTCttt                 >  2:762231/1‑149 (MQ=255)
                                                                                                                                      gAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTa                 >  1:312996/1‑150 (MQ=255)
                                                                                                                                        aaaCCGCCAGCGTTTCCGCTGGCGGTTTTCAATGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATATCAGTTa                 >  1:1943192/1‑150 (MQ=38)
                                                                                                                                        aaaCCGCCAGCGTTTCCGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTAACTTAAg               >  1:1327288/1‑150 (MQ=255)
                                                                                                                                        aaaCCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATTAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg               <  2:1290634/150‑1 (MQ=255)
                                                                                                                                        aaaCCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg               >  1:1636714/1‑150 (MQ=255)
                                                                                                                                        aaaCCGCAAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg               <  1:835218/150‑1 (MQ=255)
                                                                                                                                          aCCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAgga             >  1:371937/1‑149 (MQ=255)
                                                                                                                                           ccGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCt            >  2:452762/1‑150 (MQ=255)
                                                                                                                                           ccGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCt            >  2:1443542/1‑150 (MQ=255)
                                                                                                                                           ccGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCt            >  1:608285/1‑150 (MQ=255)
                                                                                                                                               cAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCt        >  2:1053913/1‑150 (MQ=255)
                                                                                                                                                 gCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTta      >  2:858478/1‑150 (MQ=255)
                                                                                                                                                 gCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTta      <  2:1187835/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTT‑‑TTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAA  >  NC_000913/729228‑729526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: