Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 747,895 C→G 7.5% intergenic (‑126/+26) abrB ← / ← ybgO regulator of aidB expression; inner membrane protein/putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913747,8950CG 7.5% 403.3 / 19.6 132intergenic (‑126/+26)abrB/ybgOregulator of aidB expression; inner membrane protein/putative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base C (63/59);  new base G (5/5);  total (68/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-01

Only 100 of 132 total aligned reads displayed.

CCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAA  >  NC_000913/747750‑748043
                                                                                                                                                 |                                                                                                                                                    
ccccACTCCAACCCTCGCATCCAGCGCTCCCACAGAATCTACACATCGGCGGCTCGCTCCAGCAAAACGCAACCTGGCCCAGGAAAAAATAACAGCAATCAAGGAAAAGCGGAAACTCACCAAATTTCTGAAAGAGCTGCCCTCTCcggc                                                                                                                                                  <  2:1576696/150‑1 (MQ=255)
  ccACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCcggcgg                                                                                                                                                <  1:354220/150‑1 (MQ=255)
  ccACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCcggcgg                                                                                                                                                <  2:160862/150‑1 (MQ=255)
  ccACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAAGCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCcggcgg                                                                                                                                                <  2:1171871/150‑1 (MQ=255)
   cACTGCAAAACTGGCATCCTTCGCACCCTCATTATTTTCTCTTTGGTGAATCGATACAGCAAAAAGAAAGCTGTGACAGTAAAAAATAACAGCAAACAAGTAAAAGGGGAAAATAAGCAATTTTCTGAAAGAGATTCCCTTTCCggcggc                                                                                                                                               >  2:1698322/1‑150 (MQ=255)
       gCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAg                                                                                                                                           <  2:1707151/150‑1 (MQ=255)
       gCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGACCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAg                                                                                                                                           >  2:1449571/1‑150 (MQ=255)
        cAAAACTTGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAgg                                                                                                                                          <  2:1650560/150‑1 (MQ=255)
        cAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAgg                                                                                                                                          >  1:1812673/1‑150 (MQ=255)
         aaaaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAggg                                                                                                                                         >  2:661837/1‑150 (MQ=255)
         aaaaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAggg                                                                                                                                         >  2:563748/1‑150 (MQ=255)
             cTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAGGGcatc                                                                                                                                     <  1:772655/150‑1 (MQ=255)
             cTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAGGGcatc                                                                                                                                     <  1:1577738/150‑1 (MQ=255)
               ggCATCCTTCGCACCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAGGGcatctc                                                                                                                                   <  1:684316/150‑3 (MQ=255)
                gCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATg                                                                                                                                  <  1:1842028/150‑1 (MQ=255)
                 cATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAACTCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATgg                                                                                                                                 <  1:1739922/150‑1 (MQ=255)
                 cATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATgg                                                                                                                                 >  1:1477268/1‑150 (MQ=255)
                    ccTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAGGGcatctctttca                                                                                                                              <  1:1727703/150‑8 (MQ=255)
                     cTTCTCTACCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTaaa                                                                                                                             <  2:1919287/150‑1 (MQ=255)
                     cTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTaaa                                                                                                                             >  2:1325666/1‑150 (MQ=255)
                     cTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTaaa                                                                                                                             <  2:1593217/150‑1 (MQ=255)
                       tCGCTCCCTCATTATTTTCTCTTTCGAGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATATTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATc                                                                                                                           <  2:283818/150‑1 (MQ=255)
                          cTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGt                                                                                                                        >  1:1280217/1‑150 (MQ=255)
                          cTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGt                                                                                                                        <  1:212162/150‑1 (MQ=255)
                          cTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGt                                                                                                                        >  1:1961086/1‑150 (MQ=255)
                           tCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAATTCAGtt                                                                                                                       <  1:1120481/150‑1 (MQ=255)
                            cccTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAAAAACCGCAATCAAGGAAAAAGGGAAAATCAGCAATTTTGAGAAAGAAAATCCCGTTGCGGCGGCAAAGGGCAACATGGTAAATAAGttt                                                                                                                      >  2:1977050/1‑149 (MQ=255)
                             cctacttattTTCTCTTTGGTGTATCGATACAGCATACAGAACCATGGTCCATGAAAAAATAACACAAATCAAGGAAACTGGGAAATTCAGCAATTTTCTGAAATAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTtat                                                                                                                     <  2:964803/145‑1 (MQ=255)
                                 attattTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCTAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGtt                                                                                                                 >  1:1797680/1‑150 (MQ=255)
                                     ttttCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATc                                                                                                             >  1:1009831/1‑150 (MQ=255)
                                           ctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGCTGCACTTTCCGGCGGCTAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaaa                                                                                                       >  1:129745/1‑150 (MQ=255)
                                           ctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaaa                                                                                                       <  1:1109706/150‑1 (MQ=255)
                                           ctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaaa                                                                                                       >  2:1729026/1‑150 (MQ=255)
                                              tGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAACCAGTTATAGTTAATCTTAAAAAGc                                                                                                    >  1:1974773/1‑150 (MQ=255)
                                               ggTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGca                                                                                                   >  1:992851/1‑150 (MQ=255)
                                               ggTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGca                                                                                                   <  1:1382280/150‑1 (MQ=255)
                                                gtgtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGcac                                                                                                  <  2:1812673/150‑1 (MQ=255)
                                                 tgtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGcacc                                                                                                 <  1:563748/150‑1 (MQ=255)
                                                     tCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTg                                                                                             >  2:27873/1‑150 (MQ=255)
                                                      cGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGt                                                                                            >  1:1578051/1‑150 (MQ=255)
                                                       gATACAGCATAAAGAAAGCTGGGCCATGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAATGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTa                                                                                           <  1:1901054/150‑1 (MQ=255)
                                                       gATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTa                                                                                           >  1:1097893/1‑150 (MQ=255)
                                                           cAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCATTTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATcaa                                                                                       >  1:1774279/1‑148 (MQ=255)
                                                           cAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGa                                                                                       >  1:318893/1‑150 (MQ=255)
                                                              cATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAgg                                                                                    >  1:493081/1‑150 (MQ=255)
                                                              cATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACAACTGTATCGAAgg                                                                                    >  1:1240626/1‑150 (MQ=255)
                                                                tAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGATAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCTAAGGGCATCATGGTAAATCAGTTATAGTTAAACTTAAAAAGCACCACTGTATCGAAgcgg                                                                                  >  1:294343/1‑150 (MQ=255)
                                                                 aaagaaagCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCATTTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGc                                                                                 >  1:30950/1‑150 (MQ=255)
                                                                    taaagCTGGGCCAGCAAAAAATAACAGCAATCAAGGAACAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATAAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAg                                                                              <  1:1744706/149‑1 (MQ=255)
                                                                    gaaagCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAg                                                                              >  1:1872354/1‑150 (MQ=255)
                                                                    gaaagCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAg                                                                              >  2:920214/1‑150 (MQ=255)
                                                                     aaagCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGc                                                                             <  2:425311/150‑1 (MQ=255)
                                                                           gggCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGAt                                                                       >  2:1387903/1‑150 (MQ=255)
                                                                               cAGGAAAAAATAACAGCAATCAAGGAAAAGTGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGTGGCCAGCCTCGATgggg                                                                   <  1:1391247/150‑1 (MQ=255)
                                                                               cAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATgggg                                                                   <  2:1263071/150‑1 (MQ=255)
                                                                                 ggAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGt                                                                 >  1:1559630/1‑150 (MQ=255)
                                                                                    aaaaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGcc                                                              >  1:1413854/1‑150 (MQ=255)
                                                                                    aaaaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGcc                                                              <  1:721642/150‑1 (MQ=255)
                                                                                    aaaaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGcc                                                              <  2:57608/150‑1 (MQ=255)
                                                                                      aaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTg                                                            <  1:570650/150‑1 (MQ=255)
                                                                                      aaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAAATTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTg                                                            <  1:683996/150‑1 (MQ=255)
                                                                                       aaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTgg                                                           >  1:1686106/1‑150 (MQ=255)
                                                                                           aCAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATAATGGTAATTCAGTTATAGATAATCTTAAAACGCAACACTGTATCGAAGTGGAAAGCATAGATGGGGGTTCATTGAATg                                                       >  1:87076/1‑150 (MQ=255)
                                                                                              gCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCGCGATGGGGGTGCCTGGAATGGAc                                                    <  1:1836458/150‑1 (MQ=255)
                                                                                                aaTCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGa                                                  >  2:1712774/1‑150 (MQ=255)
                                                                                                 aTCCAGTAAAAGTGGTATATCAGCAATAATCTGAAAGAGATTCCCTTTCCGGCTGTTAAGGTCTTCATTGTAACTCAGTTATAGTTAATCTTAAAAAGCCCCACTGTATCGAAGGGGCCAGCCGCGATGGGGGGGCCTGGAATGGACGaa                                                 <  2:1269103/150‑1 (MQ=255)
                                                                                                   cAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAg                                               >  1:1868178/1‑150 (MQ=255)
                                                                                                    aaGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGt                                              >  2:859160/1‑150 (MQ=255)
                                                                                                      ggAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATTCCCTTTCCGGCTGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTc                                            <  1:536228/150‑1 (MQ=255)
                                                                                                      ggAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTc                                            <  2:1923628/150‑1 (MQ=255)
                                                                                                        aaaaGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAg                                          <  1:1381998/150‑1 (MQ=255)
                                                                                                         aaaGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTTATCTTAAAACGCACCACTGTATCGAAGGGGCCCGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGc                                         >  2:1023192/1‑150 (MQ=255)
                                                                                                           aGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCg                                       <  1:1441083/150‑1 (MQ=255)
                                                                                                            ggggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGt                                      >  1:912174/1‑150 (MQ=255)
                                                                                                              ggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGTCCCGCCTCCATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGtat                                    >  2:299481/1‑150 (MQ=255)
                                                                                                              ggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGtat                                    >  2:1227637/1‑150 (MQ=255)
                                                                                                              ggAAAATCAGCAATTATCTGAAAGAGATGCCCTTTCCGGCGGCATAGGTCATCATGGAAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGTTGCCTGGAATGGACGAAAGTTCAGCCGtat                                    <  2:106424/150‑1 (MQ=255)
                                                                                                                 aaaTCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCTAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAgg                                 <  2:1961086/150‑1 (MQ=255)
                                                                                                                 aaaTCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAgg                                 <  1:1148651/150‑1 (MQ=255)
                                                                                                                 aaaTCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAgg                                 <  2:493081/150‑1 (MQ=255)
                                                                                                                    tCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAATGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGGTCAGCCGGATAGCttt                              >  1:814390/1‑150 (MQ=255)
                                                                                                                    tCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGttt                              <  2:1130254/150‑1 (MQ=255)
                                                                                                                       gCAATTTTCTGAAAGAGATGCCCTGTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAACGCACCCATGTATCGAAGGGGCCAGACTCGCGTGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTAAAGGtttgtt                           >  1:367408/1‑150 (MQ=255)
                                                                                                                        cAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCAGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTc                          >  2:1517575/1‑150 (MQ=255)
                                                                                                                        cAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGGGCCTGGAATGAACGAAAGTTCAGCCGTATAGGTTTTTTc                          >  1:1020591/1‑150 (MQ=255)
                                                                                                                          aTTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGt                        <  2:762915/150‑1 (MQ=255)
                                                                                                                          aTTTTCTGAAAGAGAAGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGt                        <  2:142848/150‑1 (MQ=255)
                                                                                                                           ttttCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTa                       <  2:1732722/150‑1 (MQ=255)
                                                                                                                                 gAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCaa                 >  2:1025879/1‑150 (MQ=255)
                                                                                                                                 gAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCaa                 >  1:635461/1‑150 (MQ=255)
                                                                                                                                   aaGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACa               <  2:477474/150‑1 (MQ=255)
                                                                                                                                   aaGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAACCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACa               <  1:976677/150‑1 (MQ=255)
                                                                                                                                     gagaAGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATg             <  2:1413854/150‑1 (MQ=255)
                                                                                                                                     atgaTGCCCTTTGCCGCCGGAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATg             >  2:1022404/3‑150 (MQ=255)
                                                                                                                                        aTGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCa          >  2:764939/1‑150 (MQ=255)
                                                                                                                                          gCCCTTTGCCGCCGGAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGacacg        >  1:1713002/1‑149 (MQ=255)
                                                                                                                                           cccTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTg       <  2:1715843/150‑1 (MQ=255)
                                                                                                                                             cTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGt     <  2:716184/150‑1 (MQ=255)
                                                                                                                                               ttCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCTGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGa   <  2:1930372/150‑1 (MQ=255)
                                                                                                                                                tCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGaa  <  1:1322494/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAA  >  NC_000913/747750‑748043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: