Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 752,065 G→C 5.9% D35E (GAC→GAG) ‡ ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913752,0650GC 5.9% 425.1 / 15.1 136D35E (GAC→GAG) ‡ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (63/64);  new base C (3/5);  total (67/69)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01

Only 100 of 137 total aligned reads displayed.

TCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGAT  >  NC_000913/751916‑752212
                                                                                                                                                     |                                                                                                                                                   
tCATTGATGCATGGAATTGTTTTGTCAACCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCgg                                                                                                                                                     <  1:852974/150‑1 (MQ=255)
  aTTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGAAATGACTCCTTTTTCCTTTAATAATGATATATCAATGCGGTc                                                                                                                                                   >  1:1534420/1‑150 (MQ=255)
   ttGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcg                                                                                                                                                  <  2:1044590/150‑1 (MQ=255)
    tGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgc                                                                                                                                                 <  2:1156850/150‑1 (MQ=255)
    tGATGCATGGAATGGGTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgc                                                                                                                                                 <  2:1807702/150‑1 (MQ=255)
      aTGCATGGAATGGTGTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCCCCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCACTGCGGTCgcgc                                                                                                                                               <  2:323544/150‑1 (MQ=255)
      aTGCATGGAATGGTGTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCgcgc                                                                                                                                               <  2:1825125/150‑1 (MQ=255)
            ggAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCTATGACTGCTTTTTCGTTTAATAATGAAATATCAATGCGCGACCGCATTGAt                                                                                                                                         <  2:156364/150‑1 (MQ=255)
            ggAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAt                                                                                                                                         <  1:306785/150‑1 (MQ=255)
            ggAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAt                                                                                                                                         <  2:1205929/150‑1 (MQ=255)
             gAATGGTTTTGTCACCCTTTTTTTGCCGGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAtt                                                                                                                                        <  2:559621/150‑1 (MQ=255)
                tGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTAt                                                                                                                                     <  1:1439006/150‑1 (MQ=255)
                   ttttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCga                                                                                                                                  <  1:1528151/150‑1 (MQ=255)
                   ttttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCga                                                                                                                                  <  2:1863760/150‑1 (MQ=255)
                   ttttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGAtatttcat                                                                                                                                  <  1:1237067/150‑8 (MQ=255)
                    tttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCgag                                                                                                                                 <  2:1837466/150‑1 (MQ=255)
                    tttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCgag                                                                                                                                 <  1:1522477/150‑1 (MQ=255)
                    tttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCgag                                                                                                                                 <  2:675257/150‑1 (MQ=255)
                        tCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACa                                                                                                                             >  1:518682/1‑150 (MQ=255)
                         cACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACAt                                                                                                                            >  2:185990/1‑150 (MQ=255)
                           cccTTTTTTTGCCCGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGTTAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCTAGAACATTc                                                                                                                          <  1:1862193/150‑1 (MQ=255)
                           cccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTc                                                                                                                          >  2:1616544/1‑150 (MQ=255)
                            ccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCa                                                                                                                         <  1:1581382/150‑1 (MQ=255)
                               ttttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGAt                                                                                                                      <  2:1042953/150‑1 (MQ=255)
                               ttttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGAt                                                                                                                      <  1:594620/150‑1 (MQ=255)
                                  tttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGa                                                                                                                   <  2:237054/150‑1 (MQ=255)
                                    tGCCAGTTAATTTTTTGCCCATTAGTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGTGCAATGACTCCTTTTTCCTTTAATAATTAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                 <  1:992154/150‑1 (MQ=255)
                                    tGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                 <  2:814763/150‑1 (MQ=255)
                                    tGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTACCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGATAACATTCAGATTGAAt                                                                                                                 >  2:1751113/1‑150 (MQ=255)
                                     gCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCCCCGCACCGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAtt                                                                                                                >  1:255164/1‑150 (MQ=255)
                                       cAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCg                                                                                                              <  1:1496402/150‑1 (MQ=255)
                                                ttttGCCCATTACTGTTTTTGTTGGTATTCACCTCAACTCTAACAACATCTTCACCGGGCGCAATGACTCCGTTTGCCTTTAATACTGAAATATCAATGCGGTCGCGCATTGGTTTATCGAGAACATTCAGATTGAATTCGACCGCCAtt                                                                                                     <  1:175504/150‑1 (MQ=255)
                                                   tGCCCTTTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTTATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGTTTGAATTCGACCGCCATTgcg                                                                                                  <  2:403092/150‑1 (MQ=255)
                                                       cATTACTGATTTTGTTGTTATTCACCGCAACTCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAg                                                                                              <  1:181423/150‑1 (MQ=255)
                                                       cATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAg                                                                                              <  1:323773/150‑1 (MQ=255)
                                                       cATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAg                                                                                              >  1:1890033/1‑150 (MQ=255)
                                                           aCTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCAt                                                                                          >  1:1852678/1‑150 (MQ=255)
                                                            cTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATc                                                                                         >  2:255587/1‑150 (MQ=255)
                                                                ttttGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTAAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATACGACCGCCATTGCGCAAGGCATCGCCa                                                                                     >  1:1128095/1‑150 (MQ=255)
                                                                ttttGATTTTATTAACCGCAAAGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCa                                                                                     <  1:1002809/150‑1 (MQ=255)
                                                                    gttgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGAc                                                                                 >  1:810080/1‑150 (MQ=255)
                                                                     ttgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGAcc                                                                                >  2:165531/1‑150 (MQ=255)
                                                                         tATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggc                                                                            <  1:1852960/150‑1 (MQ=255)
                                                                          aTTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggca                                                                           >  1:1223130/1‑150 (MQ=255)
                                                                          aTTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggca                                                                           >  1:56539/1‑150 (MQ=255)
                                                                            tCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAGTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggcagg                                                                         >  1:1244253/1‑150 (MQ=255)
                                                                              aCCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGAt                                                                       <  2:1677928/150‑1 (MQ=255)
                                                                              aCCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGAt                                                                       >  1:1951377/1‑150 (MQ=255)
                                                                                cGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATAc                                                                     >  1:1081502/1‑150 (MQ=255)
                                                                                 gCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACa                                                                    >  1:1397630/1‑150 (MQ=255)
                                                                                     cGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAag                                                                >  1:810070/1‑150 (MQ=255)
                                                                                      gCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAaga                                                               >  1:1419908/1‑150 (MQ=255)
                                                                                         aaCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagagag                                                            >  1:990784/1‑150 (MQ=255)
                                                                                          aCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATAAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGt                                                           >  1:1738803/1‑150 (MQ=255)
                                                                                           cAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTc                                                          <  2:734690/150‑1 (MQ=255)
                                                                                           aaaaaTATTCACTGGGAGCAATGACTCCTTTTTCCTTTAATTATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTc                                                          <  2:1409520/149‑1 (MQ=255)
                                                                                            aaaaTATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCg                                                         <  1:89808/150‑1 (MQ=255)
                                                                                            aaaaTATTCACCGGGAGCAATGAATCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCTCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCg                                                         >  2:50503/1‑150 (MQ=255)
                                                                                               atatTCACCGGGAGAAATGACTCCTTTTTCATTTAATAATGAAATATCAATTCGGTCGCGCATTGATTTATCGAGAACATTCAGATTTAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATa                                                      <  2:339333/150‑1 (MQ=255)
                                                                                                 atTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATaaa                                                    <  1:1722228/150‑1 (MQ=255)
                                                                                                  tgaaCCGGGCGCAATGACTCCTTGATCCCTTAATAAAGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGCCCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAat                                                   <  2:115760/147‑1 (MQ=255)
                                                                                                    cACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                 >  2:1714926/1‑86 (MQ=255)
                                                                                                    cACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAatat                                                 >  2:1401952/1‑150 (MQ=255)
                                                                                                    cACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAatat                                                 >  1:1173286/1‑150 (MQ=255)
                                                                                                     aCCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATAtt                                                >  2:1750188/1‑150 (MQ=255)
                                                                                                     aCCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATAtt                                                >  1:807877/1‑150 (MQ=255)
                                                                                                     aCCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATAtt                                                <  1:1759911/150‑1 (MQ=255)
                                                                                                      ccGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCACGATACAAAAGAGAGTCGATAAATAtta                                               >  1:1662330/1‑149 (MQ=255)
                                                                                                        gggAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGTCACTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCAc                                             <  2:257296/150‑1 (MQ=255)
                                                                                                            taaaTGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATTGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGgtg                                         <  1:1760077/148‑1 (MQ=255)
                                                                                                            gCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGgtg                                         >  1:1404934/1‑150 (MQ=255)
                                                                                                                ttactactTTTTCCTTTAATAATGAAATATCAATGCGTACTAGCATTGATTTATCGAGATCATTCAGAATGAATTCTACCGCCATAGCGCAAGACATCGCCAGGACAATGCAGGATACAAAAGAGAGTCGATAAATATTAACGCTGTcaa                                     >  2:1998567/3‑148 (MQ=255)
                                                                                                                tGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCa                                     <  2:1223130/150‑1 (MQ=255)
                                                                                                                 gACTCCTTTTTCGTTTAATAATGAAATATAAATTAGGTCGCGCATTGATTTATCGAGATCATTCATATTGAATTCGACCGCCAATGCGCAAGGCATCGCCATGACCAGGCCGGATACAAAAGAGAGTCGATAAATATTCACGgtctacaa                                    >  2:1580005/1‑144 (MQ=255)
                                                                                                                 gACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCAt                                    <  2:427129/150‑1 (MQ=255)
                                                                                                                  aCTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATa                                   >  1:1366930/1‑150 (MQ=255)
                                                                                                                     ccTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCt                                <  2:906571/150‑1 (MQ=255)
                                                                                                                      cgttttCCTTTAATAATGCAATATCATTGCGGGCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTg                               <  2:1131502/148‑1 (MQ=255)
                                                                                                                      cTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCCCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAAAATTCACCGTGTCCATACATg                               >  1:1135019/1‑150 (MQ=255)
                                                                                                                           tCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATaaa                          <  1:1896548/150‑1 (MQ=255)
                                                                                                                           tCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCAATGACCAGGCATGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATaaa                          <  1:1794497/150‑1 (MQ=255)
                                                                                                                            ccTTTAATATTGAAATATGAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGGGTCCATACCTGATAAat                         <  1:1792387/150‑1 (MQ=255)
                                                                                                                            ccTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAat                         >  2:612708/1‑150 (MQ=255)
                                                                                                                                 attattGAAATATCAATGCGGTCGCTCATTGATTTATCGAGAACATTCAGATTTTATTCGACAGCCATTGCGCTAGGCATCGCCATGACCAGGCAGTATACAAAAGAGAGTCGATAAATATTCTCTGTGTCCATACATGATAAATAtttt                    <  2:722937/145‑1 (MQ=255)
                                                                                                                                  ataatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTTATAAATATTTTa                   <  1:1099570/150‑1 (MQ=255)
                                                                                                                                   taatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTAt                  >  1:1936514/1‑150 (MQ=255)
                                                                                                                                   taatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTAt                  >  1:1183172/1‑150 (MQ=255)
                                                                                                                                    aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATg                 <  1:767236/150‑1 (MQ=255)
                                                                                                                                    aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATg                 <  2:1330004/150‑1 (MQ=255)
                                                                                                                                    aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCACCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATg                 >  1:1315328/1‑150 (MQ=255)
                                                                                                                                      tGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGaa               >  1:857779/1‑150 (MQ=255)
                                                                                                                                      tGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGaa               >  1:1311549/1‑150 (MQ=255)
                                                                                                                                        ataaatCAATGCGCGACCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAg             >  1:766322/5‑150 (MQ=255)
                                                                                                                                         taaatCAATGCGCGACCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAgg            >  2:1978437/4‑150 (MQ=255)
                                                                                                                                         aaTATCAATGCGGTCGCGCATTGAGTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGACATGACCAGGAAGGATACAAATGAGAGGCGATAAAAATTAACGGAGTCCATACCTTATAATAAATTTATGAAAgg            >  2:179226/1‑150 (MQ=255)
                                                                                                                                            atCAATGTGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAggcgg         >  1:1081061/1‑150 (MQ=255)
                                                                                                                                              cAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAACGgcggcg       >  2:1100598/1‑150 (MQ=255)
                                                                                                                                               aaTGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCga      >  2:325151/1‑150 (MQ=255)
                                                                                                                                                aTGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgat     >  1:431647/1‑150 (MQ=255)
                                                                                                                                                 tGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgatg    <  1:1640566/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGAT  >  NC_000913/751916‑752212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: