Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 752,068 G→C 6.3% R34R (CGC→CGG ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913752,0680GC 6.3% 393.9 / 15.3 128R34R (CGC→CGGybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (62/57);  new base C (3/5);  total (66/62)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 136 total aligned reads displayed.

TTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGC  >  NC_000913/751919‑752217
                                                                                                                                                     |                                                                                                                                                     
ttGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcg                                                                                                                                                       <  2:1044590/150‑1 (MQ=255)
 tGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgc                                                                                                                                                      <  2:1156850/150‑1 (MQ=255)
 tGATGCATGGAATGGGTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgc                                                                                                                                                      <  2:1807702/150‑1 (MQ=255)
  gATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTcgcg                                                                                                                                                     <  1:1513789/150‑1 (MQ=255)
   aTGCATGGAATGGTGTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCCCCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCACTGCGGTCgcgc                                                                                                                                                    <  2:323544/150‑1 (MQ=255)
   aTGCATGGAATGGTGTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCgcgc                                                                                                                                                    <  2:1825125/150‑1 (MQ=255)
        ttgAATGGTTTTGTCACCCTTTTTTTGCCAGTAAATTTTTTGCCCATTACTGATTTTGTTGTAATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGa                                                                                                                                               <  1:1907767/148‑1 (MQ=255)
         ggAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCTATGACTGCTTTTTCGTTTAATAATGAAATATCAATGCGCGACCGCATTGAt                                                                                                                                              <  2:156364/150‑1 (MQ=255)
         ggAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAt                                                                                                                                              <  1:306785/150‑1 (MQ=255)
         ggAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAt                                                                                                                                              <  2:1205929/150‑1 (MQ=255)
          gAATGGTTTTGTCACCCTTTTTTTGCCGGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAtt                                                                                                                                             <  2:559621/150‑1 (MQ=255)
          gAATGGTTTTGTCACCCTTTTTTTGACAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCTCCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGAtt                                                                                                                                             <  1:1758824/150‑1 (MQ=255)
             tGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTAt                                                                                                                                          <  1:1439006/150‑1 (MQ=255)
               gTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCACTGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCg                                                                                                                                        <  1:1832876/150‑1 (MQ=255)
                ttttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCga                                                                                                                                       <  2:1863760/150‑1 (MQ=255)
                ttttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCga                                                                                                                                       <  1:1528151/150‑1 (MQ=255)
                ttttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGAtatttcat                                                                                                                                       <  1:1237067/150‑8 (MQ=255)
                tttaGTCACCCTTTTTTTGCCATTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATAATCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGAtatttcat                                                                                                                                       <  1:188444/150‑8 (MQ=255)
                 tttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCgag                                                                                                                                      <  2:675257/150‑1 (MQ=255)
                 tttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCgag                                                                                                                                      <  2:1837466/150‑1 (MQ=255)
                     tCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACa                                                                                                                                  >  1:518682/1‑150 (MQ=255)
                      cACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACAt                                                                                                                                 >  2:185990/1‑150 (MQ=255)
                        cccTTTTTTTGCCCGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGTTAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCTAGAACATTc                                                                                                                               <  1:1862193/150‑1 (MQ=255)
                        cccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTc                                                                                                                               <  1:795114/150‑1 (MQ=255)
                        cccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTc                                                                                                                               >  2:1616544/1‑150 (MQ=255)
                         ccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCa                                                                                                                              <  1:1581382/150‑1 (MQ=255)
                            ttttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGAt                                                                                                                           <  2:1042953/150‑1 (MQ=255)
                            ttttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGAt                                                                                                                           <  1:594620/150‑1 (MQ=255)
                               tttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGa                                                                                                                        <  2:237054/150‑1 (MQ=255)
                                 tGCCAGTTAATTTTTTGCCCATTAGTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGTGCAATGACTCCTTTTTCCTTTAATAATTAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                      <  1:992154/150‑1 (MQ=255)
                                 tGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                      <  2:814763/150‑1 (MQ=255)
                                 tGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTACCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGATAACATTCAGATTGAAt                                                                                                                      >  2:1751113/1‑150 (MQ=255)
                                  gCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCCCCGCACCGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAtt                                                                                                                     >  1:255164/1‑150 (MQ=255)
                                    cAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCg                                                                                                                   <  1:1496402/150‑1 (MQ=255)
                                             ttttGCCCATTACTGTTTTTGTTGGTATTCACCTCAACTCTAACAACATCTTCACCGGGCGCAATGACTCCGTTTGCCTTTAATACTGAAATATCAATGCGGTCGCGCATTGGTTTATCGAGAACATTCAGATTGAATTCGACCGCCAtt                                                                                                          <  1:175504/150‑1 (MQ=255)
                                                tGCCCTTTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTTATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGTTTGAATTCGACCGCCATTgcg                                                                                                       <  2:403092/150‑1 (MQ=255)
                                                    cATTACTGATTTTGTTGTTATTCACCGCAACTCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAg                                                                                                   <  1:181423/150‑1 (MQ=255)
                                                    cATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAg                                                                                                   >  1:1890033/1‑150 (MQ=255)
                                                        aCTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCAt                                                                                               >  1:1852678/1‑150 (MQ=255)
                                                         cTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATc                                                                                              >  2:255587/1‑150 (MQ=255)
                                                             ttttGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTAAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATACGACCGCCATTGCGCAAGGCATCGCCa                                                                                          >  1:1128095/1‑150 (MQ=255)
                                                             ttttGATTTTATTAACCGCAAAGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCa                                                                                          <  1:1002809/150‑1 (MQ=255)
                                                                 gttgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGAc                                                                                      >  1:810080/1‑150 (MQ=255)
                                                                  ttgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGAcc                                                                                     >  2:165531/1‑150 (MQ=255)
                                                                    gttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcag                                                                                   <  1:274104/150‑1 (MQ=255)
                                                                       aTTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggca                                                                                >  1:1223130/1‑150 (MQ=255)
                                                                         tCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAGTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggcagg                                                                              >  1:1244253/1‑150 (MQ=255)
                                                                           aCCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGAt                                                                            <  2:1677928/150‑1 (MQ=255)
                                                                             cGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATAc                                                                          >  1:1081502/1‑150 (MQ=255)
                                                                              gCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACa                                                                         >  1:1397630/1‑150 (MQ=255)
                                                                               cAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACaa                                                                        >  2:1701057/1‑150 (MQ=255)
                                                                                   gCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAaga                                                                    >  1:1419908/1‑150 (MQ=255)
                                                                                      aaCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagagag                                                                 >  1:990784/1‑150 (MQ=255)
                                                                                       aCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATAAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGt                                                                >  1:1738803/1‑150 (MQ=255)
                                                                                        cAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTc                                                               <  2:734690/150‑1 (MQ=255)
                                                                                        aaaaaTATTCACTGGGAGCAATGACTCCTTTTTCCTTTAATTATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTc                                                               <  2:1409520/149‑1 (MQ=255)
                                                                                         aaaaTATTCACCGGGAGCAATGAATCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCTCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCg                                                              >  2:50503/1‑150 (MQ=255)
                                                                                               ttCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAat                                                        >  1:1565461/1‑150 (MQ=255)
                                                                                               tgaaCCGGGCGCAATGACTCCTTGATCCCTTAATAAAGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGCCCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAat                                                        <  2:115760/147‑1 (MQ=255)
                                                                                                 cACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                      <  1:1714926/86‑1 (MQ=255)
                                                                                                 cACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAatat                                                      >  1:1173286/1‑150 (MQ=255)
                                                                                                 cACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAatat                                                      >  2:1401952/1‑150 (MQ=255)
                                                                                                  aCCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATAtt                                                     >  1:807877/1‑150 (MQ=255)
                                                                                                  aCCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATAtt                                                     >  2:1750188/1‑150 (MQ=255)
                                                                                                   ccGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCACGATACAAAAGAGAGTCGATAAATAtta                                                    >  1:1662330/1‑149 (MQ=255)
                                                                                                     gggAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGTCACTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCAc                                                  <  2:257296/150‑1 (MQ=255)
                                                                                                         taaaTGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATTGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGgtg                                              <  1:1760077/148‑1 (MQ=255)
                                                                                                         gCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGgtg                                              >  1:1404934/1‑150 (MQ=255)
                                                                                                          cAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGgtgt                                             >  2:38104/1‑150 (MQ=255)
                                                                                                          aaaTGACTCATTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGAGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGgtgt                                             <  1:814262/149‑1 (MQ=255)
                                                                                                           aaTGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTc                                            <  1:579114/150‑1 (MQ=255)
                                                                                                             ttactactTTTTCCTTTAATAATGAAATATCAATGCGTACTAGCATTGATTTATCGAGATCATTCAGAATGAATTCTACCGCCATAGCGCAAGACATCGCCAGGACAATGCAGGATACAAAAGAGAGTCGATAAATATTAACGCTGTcaa                                          >  2:1998567/3‑148 (MQ=255)
                                                                                                             tGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCa                                          <  2:1223130/150‑1 (MQ=255)
                                                                                                              gACTCCTTTTTCGTTTAATAATGAAATATAAATTAGGTCGCGCATTGATTTATCGAGATCATTCATATTGAATTCGACCGCCAATGCGCAAGGCATCGCCATGACCAGGCCGGATACAAAAGAGAGTCGATAAATATTCACGgtctacaa                                         >  2:1580005/1‑144 (MQ=255)
                                                                                                              gACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCAt                                         <  2:427129/150‑1 (MQ=255)
                                                                                                               aCTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATa                                        >  1:1366930/1‑150 (MQ=255)
                                                                                                                  ccTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCt                                     <  2:906571/150‑1 (MQ=255)
                                                                                                                   cgttttCCTTTAATAATGCAATATCATTGCGGGCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTg                                    <  2:1131502/148‑1 (MQ=255)
                                                                                                                   cTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCCCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAAAATTCACCGTGTCCATACATg                                    >  1:1135019/1‑150 (MQ=255)
                                                                                                                         ccTTTAATATTGAAATATGAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGGGTCCATACCTGATAAat                              <  1:1792387/150‑1 (MQ=255)
                                                                                                                         ccTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAat                              >  2:612708/1‑150 (MQ=255)
                                                                                                                          cTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAata                             >  1:44154/1‑150 (MQ=255)
                                                                                                                              attattGAAATATCAATGCGGTCGCTCATTGATTTATCGAGAACATTCAGATTTTATTCGACAGCCATTGCGCTAGGCATCGCCATGACCAGGCAGTATACAAAAGAGAGTCGATAAATATTCTCTGTGTCCATACATGATAAATAtttt                         <  2:722937/145‑1 (MQ=255)
                                                                                                                               ataatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTTATAAATATTTTa                        <  1:1099570/150‑1 (MQ=255)
                                                                                                                                taatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTAt                       >  1:1936514/1‑150 (MQ=255)
                                                                                                                                taatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTAt                       >  1:1183172/1‑150 (MQ=255)
                                                                                                                                 aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATg                      <  2:1330004/150‑1 (MQ=255)
                                                                                                                                 aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCACCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATg                      >  1:1315328/1‑150 (MQ=255)
                                                                                                                                  tcgataaatCAATGCGCGACCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGa                     >  1:903869/8‑150 (MQ=255)
                                                                                                                                   tGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGaa                    >  1:857779/1‑150 (MQ=255)
                                                                                                                                     ataaatCAATGCGCGACCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAg                  >  1:766322/5‑150 (MQ=255)
                                                                                                                                      taaatCAATGCGCGACCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAgg                 >  2:1978437/4‑150 (MQ=255)
                                                                                                                                      aaTATCAATGCGGTCGCGCATTGAGTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGACATGACCAGGAAGGATACAAATGAGAGGCGATAAAAATTAACGGAGTCCATACCTTATAATAAATTTATGAAAgg                 >  2:179226/1‑150 (MQ=255)
                                                                                                                                       atatCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAATCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAggc                <  1:1776555/150‑1 (MQ=255)
                                                                                                                                         atCAATGTGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAggcgg              >  1:1081061/1‑150 (MQ=255)
                                                                                                                                           cAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAACGgcggcg            >  2:1100598/1‑150 (MQ=255)
                                                                                                                                            aaTGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCga           >  2:325151/1‑150 (MQ=255)
                                                                                                                                             aTGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgat          >  1:431647/1‑150 (MQ=255)
                                                                                                                                                cGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgatgat       >  1:1288237/1‑150 (MQ=255)
                                                                                                                                                     gcgcATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATgccgc  <  2:1951377/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGC  >  NC_000913/751919‑752217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: