Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 832,365 A→C 6.1% intergenic (+129/+103) rhlE → / ← ybiA ATP‑dependent RNA helicase/DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913832,3650AC 6.1% 341.8 / 10.8 115intergenic (+129/+103)rhlE/ybiAATP‑dependent RNA helicase/DUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base A (54/54);  new base C (2/5);  total (56/59)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-01

Only 100 of 119 total aligned reads displayed.

AAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGC  >  NC_000913/832216‑832514
                                                                                                                                                     |                                                                                                                                                     
aaaCCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCATGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATaa                                                                                                                                                       <  1:107549/150‑1 (MQ=255)
 aaCCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAg                                                                                                                                                      <  2:59873/150‑1 (MQ=255)
  aCCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAgg                                                                                                                                                     <  1:2012216/150‑1 (MQ=255)
   ccTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCCGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGc                                                                                                                                                    >  1:572529/1‑150 (MQ=255)
        cGCTGCGCAGTAATCTTTTATGCCGGGCTATACCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTa                                                                                                                                               <  1:1867623/150‑1 (MQ=255)
           tGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                            <  1:1513515/150‑1 (MQ=255)
           tGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                            >  1:202258/1‑150 (MQ=255)
               cAGTAATCTTTTATGCCGGGCTATGACCGGCATCAGGCTGATGAACAAACTCAAAACTGCCTGATGCGCTACGATTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACTCCGCa                                                                                                                                        >  2:242426/1‑150 (MQ=255)
                  tAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                     <  1:1750969/150‑1 (MQ=25)
                  tAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                     <  2:1533482/150‑1 (MQ=25)
                     tCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                  <  1:1059000/150‑1 (MQ=21)
                     tCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                  <  1:1299311/150‑1 (MQ=21)
                      cTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTATGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                 >  2:1714752/1‑150 (MQ=255)
                          tATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTc                                                                                                                             <  1:1553131/150‑1 (MQ=255)
                           aTGCCGGGCTATGCCCGGCATCATGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTca                                                                                                                            <  1:1290074/150‑1 (MQ=255)
                           aTGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTca                                                                                                                            >  2:131627/1‑150 (MQ=255)
                            tGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcac                                                                                                                           <  1:347474/150‑1 (MQ=255)
                            tGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcac                                                                                                                           >  1:1472635/1‑150 (MQ=255)
                             gCCGGGCTATGCCCGGCATCAGGCTGATTAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcaca                                                                                                                          <  1:139155/150‑1 (MQ=255)
                              ccGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCacaa                                                                                                                         >  2:93799/1‑150 (MQ=255)
                                  gCTATGCCGGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg                                                                                                                     <  1:807385/150‑1 (MQ=255)
                                  gCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg                                                                                                                     <  1:1440184/150‑1 (MQ=255)
                                   cTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGc                                                                                                                    <  1:1324524/150‑1 (MQ=255)
                                        cccGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTg                                                                                                               >  2:1610077/1‑150 (MQ=255)
                                          cGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTc                                                                                                             <  2:855649/150‑1 (MQ=255)
                                             cATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGc                                                                                                          <  1:1420229/150‑1 (MQ=255)
                                             cATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGc                                                                                                          <  2:1898618/150‑1 (MQ=255)
                                               acAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCAAATCAGGCCTACGTGACCTCTGCAATATATTGAATTTGCATGCTTATGTAGGCCGGATAAGGCGTTTACGCCGCATACGGCATTTCACAACAAGCACTTGTCAGCaa                                                                                                        >  1:1079128/2‑150 (MQ=21)
                                                 aGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAAtt                                                                                                      <  1:416195/150‑1 (MQ=255)
                                                  ggCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAAttt                                                                                                     <  2:1191047/150‑1 (MQ=255)
                                                    cTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTga                                                                                                   <  2:131201/150‑1 (MQ=255)
                                                     tgatgaACAAACGCAAAACTGCCTGATGCGCTACGCTTCTCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTgag                                                                                                  <  1:586804/150‑1 (MQ=255)
                                                     tgatgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTgag                                                                                                  >  2:1458112/1‑150 (MQ=255)
                                                      gatgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTgaga                                                                                                 >  2:1731531/1‑150 (MQ=255)
                                                      gatgaACAAACGAAAAACTGCCTGATGCGCTAAGCTTATAATTCATACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTgaga                                                                                                 <  2:1131227/150‑1 (MQ=25)
                                                       atgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGaa                                                                                                <  2:708302/150‑1 (MQ=255)
                                                        tgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGaaa                                                                                               >  2:159619/1‑149 (MQ=255)
                                                        tgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAac                                                                                               >  1:1652526/1‑150 (MQ=255)
                                                        tgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAac                                                                                               <  1:342269/150‑1 (MQ=255)
                                                           aCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACg                                                                                            <  2:640170/150‑1 (MQ=255)
                                                           aCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGGCAGCAATTTGTGAGCACg                                                                                            >  1:1869479/1‑150 (MQ=255)
                                                              aaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGGCAGCAATTTGAGAACACGGGa                                                                                         >  2:450816/1‑150 (MQ=255)
                                                                cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGGATCCGGCATTTCACAACAAGCACTTGTCAGCAATATGAGAACACGGGaaa                                                                                       >  1:196872/1‑150 (MQ=255)
                                                                cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGaaa                                                                                       <  1:1871362/150‑1 (MQ=255)
                                                                 gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGaaaa                                                                                      >  2:539633/1‑150 (MQ=255)
                                                                      aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAAttt                                                                                 <  1:1806793/150‑1 (MQ=255)
                                                                      aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCAGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAAttt                                                                                 >  1:52712/1‑150 (MQ=255)
                                                                       cTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTa                                                                                >  1:564838/1‑150 (MQ=255)
                                                                       cTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTa                                                                                <  1:1959973/150‑1 (MQ=255)
                                                                         gCCTGATGCGCTACGCTGATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATc                                                                              <  2:103555/150‑1 (MQ=255)
                                                                          ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGTATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCt                                                                             >  1:1063505/1‑150 (MQ=255)
                                                                           cTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTg                                                                            <  1:717571/150‑1 (MQ=255)
                                                                             gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtt                                                                          >  1:1251358/1‑150 (MQ=255)
                                                                             gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtt                                                                          <  2:676019/150‑1 (MQ=255)
                                                                               tGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTGGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCAATTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtttt                                                                        <  2:1227132/150‑1 (MQ=255)
                                                                               tGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGtttt                                                                        <  2:352537/150‑1 (MQ=255)
                                                                                gcgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTa                                                                       <  1:1475828/150‑1 (MQ=255)
                                                                                 cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCGTCCGGCATTTCACAACAAGCACTTGACAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAc                                                                      >  1:733994/1‑150 (MQ=255)
                                                                                     aCGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGcc                                                                  <  1:168929/150‑1 (MQ=255)
                                                                                      cGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGccc                                                                 >  2:1920854/1‑150 (MQ=255)
                                                                                      cGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGccc                                                                 >  1:1669905/1‑150 (MQ=255)
                                                                                         ttATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACAGCCCATa                                                              >  1:768257/1‑150 (MQ=255)
                                                                                           aTCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAg                                                            <  1:739597/150‑1 (MQ=255)
                                                                                           aTCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAg                                                            <  2:213285/150‑1 (MQ=255)
                                                                                             cAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGAATACGTCATTTCACAACAAGCACTAGTCAGCAATTAGAGAACACGGGAAAAAATTTATCTCTTTTACCGCCCATAAGcc                                                          >  1:87916/1‑150 (MQ=37)
                                                                                                  cTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGATAAAATTTATCTGTTTTCCCGCCCATAAGCCACCga                                                     >  2:508350/1‑150 (MQ=255)
                                                                                                  cTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCga                                                     <  2:630656/150‑1 (MQ=255)
                                                                                                   tACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCgag                                                    >  1:1004212/1‑150 (MQ=255)
                                                                                                   tACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCgag                                                    <  1:720475/150‑1 (MQ=255)
                                                                                                     cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGAc                                                  <  1:1731531/150‑1 (MQ=255)
                                                                                                       tGAACTCTGCAATATATTGATTTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGCGACGt                                                >  2:1047929/1‑150 (MQ=255)
                                                                                                          aCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTAc                                             <  1:1620278/150‑1 (MQ=255)
                                                                                                           ctctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAATCCACCGAGACGTTACt                                            >  2:462750/1‑150 (MQ=255)
                                                                                                           ctctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACt                                            >  1:1415058/1‑150 (MQ=255)
                                                                                                             ctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACttg                                          <  1:93799/150‑2 (MQ=255)
                                                                                                                    atcttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAg                                   <  2:228450/147‑1 (MQ=255)
                                                                                                                    atatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAg                                   <  1:1969613/150‑1 (MQ=255)
                                                                                                                       ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCa                                >  1:395494/1‑150 (MQ=255)
                                                                                                                         gAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAAt                              >  2:1189682/1‑150 (MQ=255)
                                                                                                                           aTTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTg                            >  1:786148/1‑150 (MQ=255)
                                                                                                                               gCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTcg                        >  1:1888355/1‑150 (MQ=255)
                                                                                                                                  tGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCgcgc                     <  2:754949/150‑1 (MQ=255)
                                                                                                                                    cTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCaa                   >  1:838490/1‑150 (MQ=255)
                                                                                                                                    cTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATACGGCATTTAACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCaa                   >  2:741598/1‑150 (MQ=255)
                                                                                                                                     ttttGTAGGCCGGATAAGGCGTTTACGCCGCTTCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAAc                  <  1:1091165/150‑1 (MQ=255)
                                                                                                                                     ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAAc                  <  1:1442662/150‑1 (MQ=255)
                                                                                                                                         gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCa              >  2:1237604/1‑150 (MQ=255)
                                                                                                                                          tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCAt             >  1:1706650/1‑150 (MQ=255)
                                                                                                                                          tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCAt             >  1:324423/1‑150 (MQ=255)
                                                                                                                                          aatgCCGGATGCGGCGTAAACGCCTTATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCAt             >  2:1334237/4‑150 (MQ=255)
                                                                                                                                          aatgCCGGATGCGGCGTAAACGCCTTATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCAt             >  2:591920/4‑150 (MQ=255)
                                                                                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTa           >  1:706530/1‑150 (MQ=255)
                                                                                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTa           >  2:768237/1‑150 (MQ=255)
                                                                                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTa           >  1:1993153/1‑150 (MQ=255)
                                                                                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCAATTCACAACAAGCACTTGTCAGCAATTAGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTCCTTCTCTATAGCCAATTGTTCGCGCAACTCCATTa           >  2:1312114/1‑150 (MQ=255)
                                                                                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTaa          >  1:256995/1‑150 (MQ=255)
                                                                                                                                               cGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTaaaa        <  1:131627/150‑1 (MQ=255)
                                                                                                                                                 gATAAGGCGTTTACGCCTCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATGTGTTTTACGGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAgg      <  2:1079128/150‑1 (MQ=255)
                                                                                                                                                   tAAGGCGTTTACGCCGCATCCGGCATTTCACAACATGCACTTGTCAGCAATTTGAGAACACGGGAAAAATGTTATCTGTTTTACCGCGCATAAGACACCGAGACGTTACTTCTCTTTAGCCAATTGTTCGCGCCTCTCCAATAAAAGGTa    >  1:325091/1‑150 (MQ=255)
                                                                                                                                                     aGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGc  <  1:621009/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGC  >  NC_000913/832216‑832514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: