Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 857,578 C→G 5.4% intergenic (+23/+218) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913857,5780CG 5.4% 399.0 / 3.5 134intergenic (+23/+218)ybiT/ybiUABC‑F family putative regulatory ATPase/DUF1479 family protein
Reads supporting (aligned to +/- strand):  ref base C (68/56);  new base G (3/5);  total (72/62)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

CTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAGCGC  >  NC_000913/857429‑857726
                                                                                                                                                     |                                                                                                                                                    
cTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATAc                                                                                                                                                      >  1:1951368/1‑150 (MQ=255)
  ttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTg                                                                                                                                                    >  1:1334027/1‑150 (MQ=255)
  ttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTg                                                                                                                                                    >  1:612538/1‑150 (MQ=255)
    gTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGCCCCGCATTCTGGAAATCCCCCCGGAACGCGTGATCGACTATATCGGTAATTACGAAGATTACCTTCGTAGTAAAGGGATCGAGTAAGTGATTATCATTCACCCCGAAACtcac                                                                                                                                                  >  2:498785/1‑147 (MQ=255)
    gTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTc                                                                                                                                                  >  1:185011/1‑150 (MQ=255)
      ttCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgg                                                                                                                                                >  1:1340444/1‑150 (MQ=255)
      ttCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGAGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGAGCGAGTAAGTGATTATCCGTCACCCCGCTCCTGGCgg                                                                                                                                                >  1:1243723/1‑150 (MQ=255)
       tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                               >  2:1806890/1‑150 (MQ=255)
        cacaCGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgggg                                                                                                                                              <  1:1117477/150‑1 (MQ=255)
        cacaCGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGAAACTGTCgggg                                                                                                                                              >  1:1472952/1‑150 (MQ=255)
         acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGt                                                                                                                                             >  2:1617174/1‑150 (MQ=255)
         acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGt                                                                                                                                             >  1:171933/1‑150 (MQ=255)
         acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGAGACTGTCGGGGt                                                                                                                                             >  1:403001/1‑150 (MQ=255)
          cacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTg                                                                                                                                            >  2:158913/1‑150 (MQ=255)
            cGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTGAc                                                                                                                                          <  1:839346/150‑1 (MQ=255)
            cGACCGGGAGTTCGTAAGCTCCCTCGCGACCCGCATTCTGGAAATCACCCCGGAACTCGTGATCGACTTTAGCTGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAc                                                                                                                                          <  2:62011/150‑1 (MQ=255)
             gACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTGAcg                                                                                                                                         <  1:723966/150‑1 (MQ=255)
                cGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcggcg                                                                                                                                      >  2:1524200/1‑150 (MQ=255)
                 gTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGCTACTGTCGGGGTGAcggcgg                                                                                                                                     >  1:1790220/1‑150 (MQ=255)
                    aGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtg                                                                                                                                  <  2:753083/150‑1 (MQ=255)
                     gTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAAAGCGTCATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATACGTCACCCCGATACTGTAGGCGTGACGGCGGAtga                                                                                                                                 >  1:416150/1‑150 (MQ=255)
                      ttCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgat                                                                                                                                >  2:298201/1‑150 (MQ=255)
                       tCGTAAGCTCCCTGGCGACCACCATTCTGGAAATCCCCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTCCCTGCGTAGTAAAGCGAGCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgatg                                                                                                                               <  1:322043/150‑1 (MQ=255)
                       gcGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGATGATTACCTGCGTAGAAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgatg                                                                                                                               <  2:733536/149‑1 (MQ=255)
                        cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgatgc                                                                                                                              >  1:411262/1‑149 (MQ=255)
                         gTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGTTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGGCGTGGTGACGGCGGCTGATGAc                                                                                                                             >  2:684775/1‑150 (MQ=255)
                             ccTCCTTGGGGACCCGCATTCTGTAAATCACCCCGTATCGGGGTATCTACTTTTGGGTTAATTACTAATTTAACGTGCTTTTTAATGGGATCGAGTATGTTATTTTCCGTCACCCCGATAGTGTCGGGGTTACGGCGGCTGATGACAAAt                                                                                                                         <  2:1743119/149‑1 (MQ=255)
                                    ggCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGTTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAAt                                                                                                                  <  1:565879/150‑1 (MQ=255)
                                      cGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAg                                                                                                                <  1:1198247/150‑1 (MQ=255)
                                        aCCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGcc                                                                                                              <  2:512233/150‑1 (MQ=255)
                                        aCCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGcc                                                                                                              >  2:1027126/1‑150 (MQ=255)
                                         cccGCATTCTGGAAATCACCCCGGACCGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCt                                                                                                             >  2:1241980/1‑150 (MQ=255)
                                          ccGCATCCTGGAAATCACCCCGGAACGCGTGAGCGACTTTCGCGGTAATTACGAAGATAACCTGCGTAGTAAAGGGATCGAGTAAGTTATTATCCGCCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTg                                                                                                            <  1:2014949/150‑1 (MQ=255)
                                            gCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAACATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGAt                                                                                                          <  2:1281784/150‑1 (MQ=255)
                                             cATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATAATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATg                                                                                                         <  2:1293667/150‑1 (MQ=255)
                                               ttCTGGACATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAACGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATgcg                                                                                                       <  2:145978/150‑1 (MQ=255)
                                                 cTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCt                                                                                                     >  2:559832/1‑150 (MQ=255)
                                                  tGGAACTCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCGGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTa                                                                                                    <  2:150511/150‑1 (MQ=255)
                                                  tGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTa                                                                                                    >  1:94123/1‑150 (MQ=255)
                                                    gAAAGCCCCCCGCCACGCGTGATCGACTTTAGCGGTAAATACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACg                                                                                                  <  2:223300/150‑1 (MQ=255)
                                                     aaaTCACCCCGGACCGCGTGATCGACTTTCGCGGAAATTCCGAAGATTACCTGCGTAGTAAAGGTATCGAGTAATTGATTAGCCGGCCCCCCTACACTGCCGGGGGTACGGCGGCTGATGACAAATGCAAAATACCCTGATGCGCTACGc                                                                                                 <  1:191287/150‑1 (MQ=255)
                                                      aaTCACCCCGGAACACGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGTGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCt                                                                                                <  1:124856/150‑1 (MQ=255)
                                                        tCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTa                                                                                              <  2:210981/150‑1 (MQ=255)
                                                               gtaaCGCGTGATCGACTTTTGCGGTAATTACGAAGATTACCTGCGTAGTAATGGGATCGATTAAGTGATTATCCGTCACCCCGATACTGTAGGTGTGACGGCGGCTGATGACAAATTCAAAATAGCCTTATTCGCTACGCTTATCAGGcc                                                                                       <  2:1169466/148‑1 (MQ=255)
                                                                   cgcgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACg                                                                                   >  1:1160671/1‑150 (MQ=255)
                                                                    gcgTGATCGAGTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAATTGATTATCCGTCATCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGc                                                                                  <  2:219948/150‑1 (MQ=255)
                                                                    gcgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGc                                                                                  >  1:481212/1‑150 (MQ=255)
                                                                     cgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCa                                                                                 >  1:1246774/1‑150 (MQ=255)
                                                                      gTGAACGACTTTAGCGTTAATTACTAATATAACCTGCGTAGTAACGGGACCGAGTAAGTGATTATCCGTCAACCCGTTACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAg                                                                                <  2:790962/150‑1 (MQ=37)
                                                                        gATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCCGGCCTACGCATTa                                                                              >  2:1768871/1‑150 (MQ=255)
                                                                          tCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTTTCAGGCCTACGCAGTAcc                                                                            >  1:76649/1‑150 (MQ=255)
                                                                            gACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTg                                                                          >  2:907965/1‑150 (MQ=255)
                                                                             aCTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGc                                                                         >  2:1684921/1‑150 (MQ=255)
                                                                              cTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTgaa                                                                        >  1:8536/1‑148 (MQ=255)
                                                                                ttAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGAGCGAGTAAGTGATTATCCGGCACCCCGATCCTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAat                                                                      <  1:557010/150‑1 (MQ=255)
                                                                                 tAGCGGTATTTACTAAGATTACCTGCGTAGTAAAGGGATCGAGTATGTGATTTTCCGTCACCCCGATACTGTCGGGTTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAata                                                                     <  1:482995/150‑1 (MQ=38)
                                                                                  aGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAatat                                                                    >  2:541755/1‑150 (MQ=255)
                                                                                   gCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAatata                                                                   >  1:1110455/1‑150 (MQ=255)
                                                                                    ggttatttACTAAGATTACCTGCTTTGAAAAGGGATCGTGTATGTTTTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAatatat                                                                  <  2:703339/144‑1 (MQ=37)
                                                                                     ggTAATTACGAACATTCCCTGCGTCGAAAAGGGATCGAGTAAGTGATTATCCGCCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                                 <  1:520216/150‑1 (MQ=38)
                                                                                             cGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                         <  2:527029/150‑1 (MQ=255)
                                                                                             cGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                         >  1:1528110/1‑150 (MQ=255)
                                                                                             cGAAGATTACCTGCGTAGAAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                         <  2:1156249/150‑1 (MQ=255)
                                                                                              gAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGTGTGACGGCGGCTGATTACAAATTCAAAATAGCCTGATGCGCTACTATTATCAGGCCTACGCAGTACCTGCAATATATTTAATCTGCa                                                        <  1:1379483/150‑1 (MQ=255)
                                                                                                acaTTCCCGGCGTCTTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAgg                                                      <  2:1953299/148‑1 (MQ=255)
                                                                                                aGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAgg                                                      <  1:737558/150‑1 (MQ=255)
                                                                                                      ccTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTg                                                >  1:191624/1‑150 (MQ=255)
                                                                                                       cTGCGTACTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGTCTGATGACAACTGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGt                                               <  2:278393/150‑1 (MQ=255)
                                                                                                             tgtaATGGGATCGAGTAAGTTATTATCCGTCACCCCTATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                         <  2:1260229/149‑1 (MQ=255)
                                                                                                             agtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                         <  2:1389538/150‑1 (MQ=255)
                                                                                                             agtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                         >  2:44775/1‑150 (MQ=255)
                                                                                                               taAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGATTCTGCAGGACTTTGTAGGCCGGa                                       >  2:332697/1‑150 (MQ=255)
                                                                                                                aaaGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGATGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACATGCAATATATTTAATCTGAAGTACTTTGTAGGCCGGAt                                      >  2:707076/1‑150 (MQ=38)
                                                                                                                   gggATCGAGTAAGTGATTATCCGGCACCCCCATACTGACGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAg                                   <  2:494999/150‑1 (MQ=255)
                                                                                                                      aTCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCg                                <  1:305064/150‑1 (MQ=255)
                                                                                                                      aTCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCg                                >  1:1482950/1‑150 (MQ=255)
                                                                                                                       tCGTGTAAGTTATTATCCGTCACCCCGAAACTGTCGGGGTTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGt                               <  2:612538/150‑1 (MQ=255)
                                                                                                                       gtgAGTAAGTGATTATCCGTCACCCCGATACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATCGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGt                               <  1:1201345/148‑1 (MQ=255)
                                                                                                                        ggAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATTTATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGtt                              <  2:1247968/149‑1 (MQ=255)
                                                                                                                        cGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGtt                              >  2:468554/1‑150 (MQ=255)
                                                                                                                           gTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAc                           >  1:1079857/1‑150 (MQ=255)
                                                                                                                               gTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccg                       >  1:383223/1‑150 (MQ=255)
                                                                                                                                tGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccgc                      <  1:298201/150‑1 (MQ=255)
                                                                                                                                 gATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCa                     <  2:1340444/150‑1 (MQ=255)
                                                                                                                                 gATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCa                     <  2:1333807/150‑1 (MQ=255)
                                                                                                                                  aTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAGCGCCGCAt                    >  1:267632/1‑150 (MQ=255)
                                                                                                                                  aTTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCAt                    >  1:1413335/1‑150 (MQ=255)
                                                                                                                                    tATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCa                  >  2:129019/1‑150 (MQ=255)
                                                                                                                                      gccGTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAgg                >  1:1700455/2‑150 (MQ=255)
                                                                                                                                         gTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGATTCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAt             >  2:327889/1‑150 (MQ=255)
                                                                                                                                         gTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAt             >  1:1530272/1‑150 (MQ=255)
                                                                                                                                           cACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGGAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa           <  2:307216/150‑1 (MQ=255)
                                                                                                                                           cACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGAAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTa           >  2:1037941/1‑150 (MQ=255)
                                                                                                                                            aCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTaa          >  1:672251/1‑150 (MQ=255)
                                                                                                                                             ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc         <  2:268820/150‑1 (MQ=255)
                                                                                                                                             ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACGTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc         >  1:371158/1‑150 (MQ=255)
                                                                                                                                              cccGATACTGTCGGGGTGACGGCGGCTGATTACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACa        <  2:189910/150‑1 (MQ=255)
                                                                                                                                               ccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACaa       >  1:231710/1‑150 (MQ=255)
                                                                                                                                               ccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACaa       >  1:185717/1‑150 (MQ=255)
                                                                                                                                                    aCTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAgcgc  <  2:924942/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAGCGC  >  NC_000913/857429‑857726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: