BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	221 [0]	[0] 221	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	211 (1.570)	137/290	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
*	?	NC_000913	1300698 =	0 (0.000)	211 (1.570)	137/290	0.0	100%	intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Only 100 of 221 total aligned reads displayed.

AACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAA  >  NC_000913/1300549‑1300847

cttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                       >  2:2004835‑M2/150‑150 (MQ=255)

cttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                       >  2:722147‑M2/150‑150 (MQ=255)

cttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggaagaggaaagtgctaaa                                                                                                                                                       >  2:871169‑M2/150‑150 (MQ=255)

attctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                       <  1:824393‑M2/1‑1 (MQ=255)

 ttctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaat                                                                                                                                                      <  1:674958‑M2/2‑1 (MQ=255)

  tctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaata                                                                                                                                                     <  1:1356530‑M2/3‑1 (MQ=255)

  tctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaata                                                                                                                                                     >  1:635539‑M2/148‑150 (MQ=255)

  tctcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcatttggtagaggaaagtgataaata                                                                                                                                                     >  1:2036868‑M2/148‑150 (MQ=255)

      aaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                                 <  1:1733518‑M2/7‑1 (MQ=255)

        attacagttagttataaggatttccttcactgcttctcctcaccataatgtaatttacgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgataaATAATAATc                                                                                                                                               <  2:1078045‑M2/9‑1 (MQ=255)

           acagttagttataaggatttccttatctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAt                                                                                                                                            <  1:21501‑M2/12‑1 (MQ=255)

           acagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAt                                                                                                                                            >  2:1751835‑M2/139‑150 (MQ=255)

              gttagttataaggatttccttaactgcttctcctcaccatcatgttattttctccacatcataatgcttgtcttgatgaagaataattgaaatgatattattaattccactgcctttggtagatgaatgtgataaATAATAATCAATTGt                                                                                                                                         >  1:272822‑M2/136‑150 (MQ=255)

              gttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                         >  2:52771‑M2/136‑150 (MQ=255)

              gttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataaatgaaatgatattattaattccactgactttggtagaggaaagtgctaaATAATAATCATTTGt                                                                                                                                         >  2:1645629‑M2/136‑150 (MQ=255)

                tagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTa                                                                                                                                       >  1:1124711‑M2/134‑150 (MQ=255)

                   ttataaggatttacttatcttcttctcctcaccatcatgttattttctcaacatcataatcctgggcttgctgaagattaattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAat                                                                                                                                    <  2:1344756‑M2/20‑1 (MQ=255)

                    tataangatttccttaanngcttctcctnaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattcaactgccttttgtagatgaaagttctaaATAATAATCAATTGTTAAatt                                                                                                                                   >  2:1617184‑M2/130‑150 (MQ=255)

                    tataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatt                                                                                                                                   >  2:269594‑M2/130‑150 (MQ=255)

                     ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaattgctaaATAATAATCAATTGTTAAatta                                                                                                                                  >  1:789375‑M2/129‑150 (MQ=255)

                      taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                                 >  2:682534‑M2/128‑150 (MQ=255)

                        aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtg                                                                                                                               >  1:1862425‑M2/126‑150 (MQ=255)

                        aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtg                                                                                                                               <  1:1462933‑M2/25‑1 (MQ=255)

                         ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                              >  1:206233‑M2/125‑150 (MQ=255)

                          gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                             >  2:1469964‑M2/124‑150 (MQ=255)

                          gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAAATATCAATTGTtaaattattttt                                                                                                                             >  2:1011132‑M2/124‑147 (MQ=255)

                           atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGc                                                                                                                            <  2:2071263‑M2/28‑1 (MQ=255)

                            ttaccttaactgcttctgctcaccatcatgttaatttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCa                                                                                                                           <  2:1209930‑M2/29‑1 (MQ=255)

                              tccttaactgcttctcctctccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAtt                                                                                                                         >  2:494973‑M2/120‑150 (MQ=255)

                              tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAtt                                                                                                                         <  2:21369‑M2/31‑1 (MQ=255)

                                cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCattta                                                                                                                       >  2:2230998‑M2/118‑149 (MQ=255)

                                  taactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCac                                                                                                                     <  2:737801‑M2/35‑1 (MQ=255)

                                           ctcctcaccatcatgttattttcgccacatcataatccttggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                            <  2:222225‑M2/44‑1 (MQ=255)

                                           ctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                            >  2:1782176‑M2/107‑150 (MQ=255)

                                               tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTa                                                                                                        <  1:1473316‑M2/48‑1 (MQ=255)

                                                caccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATGATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTaa                                                                                                       >  1:914137‑M2/102‑150 (MQ=255)

                                                caccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCCCTACTGGAACTGTaa                                                                                                       >  2:1684409‑M2/102‑150 (MQ=255)

                                                 accatcatgtttttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAAt                                                                                                      <  2:129784‑M2/50‑1 (MQ=255)

                                                  ccatcatgttattttcgccacatcataatcctgttcttgctgaataataattgaaattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                                     <  1:2192510‑M2/51‑1 (MQ=255)

                                                  ccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTGATc                                                                                                     <  2:2138881‑M2/51‑1 (MQ=255)

                                                  ccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                                     >  1:548346‑M2/100‑150 (MQ=255)

                                                  ccatcatgttattttcgccacatcataatcctcggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAATGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                                     <  2:424863‑M2/51‑1 (MQ=255)

                                                     tcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGa                                                                                                  >  1:1940027‑M2/97‑150 (MQ=255)

                                                     tcatgttattttcgccaaatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGa                                                                                                  <  1:2049325‑M2/54‑1 (MQ=255)

                                                       atgttattttcgccacatcataatcctgggcttgctgaagattaattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                                >  2:818178‑M2/95‑150 (MQ=255)

                                                        tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                               >  1:1493083‑M2/94‑150 (MQ=255)

                                                           tattttcgccacatcataatcctgggcttgctgaagaataattgaaattatattattaattccactgcctttggtagaggaaagtgctaaTTAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                            <  1:736262‑M2/60‑1 (MQ=255)

                                                            attttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                           <  1:507315‑M2/61‑1 (MQ=255)

                                                            attttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                           >  1:2007670‑M2/90‑150 (MQ=255)

                                                              tttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                         <  1:570249‑M2/63‑1 (MQ=255)

                                                                 cgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                                      >  1:1061720‑M2/85‑150 (MQ=255)

                                                                  gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                     <  1:1172062‑M2/67‑1 (MQ=255)

                                                                   ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                                    <  1:1311643‑M2/68‑1 (MQ=255)

                                                                         cataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCt                                                                                   >  2:3639‑M2/77‑145 (MQ=255)

                                                                         cataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCt                                                                                   <  1:3639‑M2/69‑1 (MQ=255)

                                                                         cataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGcc                                                                              <  1:1621832‑M2/74‑1 (MQ=255)

                                                                             atcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaactgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtc                                                                          <  2:1588485‑M2/78‑1 (MQ=255)

                                                                                ctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATctct                                                                       >  2:755404‑M2/70‑150 (MQ=255)

                                                                                   ggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                                    <  2:1735105‑M2/84‑1 (MQ=255)

                                                                                    gcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                                   >  2:236654‑M2/66‑150 (MQ=255)

                                                                                       tgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                                >  1:424313‑M2/63‑150 (MQ=255)

                                                                                         ctgaagaataattgaaattctattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattg                                                              <  1:170373‑M2/90‑1 (MQ=255)

                                                                                             agaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaatt                                                          <  1:588305‑M2/94‑1 (MQ=255)

                                                                                              taataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattg                                                         <  1:1588164‑M2/95‑1 (MQ=255)

                                                                                               aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGAATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgatttga                                                        >  1:352541‑M2/55‑150 (MQ=255)

                                                                                                  aattgaaatgatattattaattccactgcctttggtagaggaaagttataaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAg                                                     <  2:1922897‑M2/99‑1 (MQ=255)

                                                                                                      gaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATAAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTTATTGAATTGAAAGAtgt                                                 >  1:2091213‑M2/48‑150 (MQ=255)

                                                                                                        aatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                               >  2:1171281‑M2/46‑150 (MQ=255)

                                                                                                         atgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGTAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttg                                              <  2:1401483‑M2/106‑1 (MQ=255)

                                                                                                            atattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATGTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTa                                           <  2:365897‑M2/109‑1 (MQ=255)

                                                                                                                 ttttattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGTATTTCACTACTTGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCa                                      <  2:1942708‑M2/114‑1 (MQ=255)

                                                                                                                   taattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATg                                    <  2:1246973‑M2/116‑1 (MQ=255)

                                                                                                                    aattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATgg                                   >  1:1980757‑M2/34‑150 (MQ=255)

                                                                                                                     attccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGa                                  >  2:1497623‑M2/33‑150 (MQ=255)

                                                                                                                       tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGATTTGAAAGATGTTTGTTAAGGCATGGATg                                >  2:1660041‑M2/31‑150 (MQ=255)

                                                                                                                       tccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATg                                >  1:720855‑M2/31‑150 (MQ=255)

                                                                                                                           ctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGACAAGATAGACATGCTTAGCCAATCTCTATGTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                            >  1:603490‑M2/27‑150 (MQ=255)

                                                                                                                           ctgcctttggtagaggaaagtgctaaAAAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                            >  2:850880‑M2/27‑150 (MQ=255)

                                                                                                                           atgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                            <  1:1772889‑M2/124‑1 (MQ=255)

                                                                                                                              cctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                         >  1:1201947‑M2/24‑150 (MQ=255)

                                                                                                                               ctttggcagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                        <  1:871169‑M2/128‑1 (MQ=255)

                                                                                                                                tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata                       <  1:1833638‑M2/129‑1 (MQ=255)

                                                                                                                                tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata                       <  1:494973‑M2/129‑1 (MQ=255)

                                                                                                                                   ggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt                    >  2:588386‑M2/19‑150 (MQ=255)

                                                                                                                                      agaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt                 <  2:1862425‑M2/135‑1 (MQ=255)

                                                                                                                                      agaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt                 >  1:1823148‑M2/16‑150 (MQ=255)

                                                                                                                                       gaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTctt                >  1:229760‑M2/15‑149 (MQ=255)

                                                                                                                                         ggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGAt              >  2:1225302‑M2/13‑150 (MQ=255)

                                                                                                                                           aaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAc            <  1:1682117‑M2/140‑1 (MQ=255)

                                                                                                                                             agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACgg          >  2:1065851‑M2/9‑150 (MQ=255)

                                                                                                                                             agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACgg          <  1:1922747‑M2/142‑1 (MQ=255)

                                                                                                                                             agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACgg          >  1:1278378‑M2/9‑150 (MQ=255)

                                                                                                                                             agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCATAGCCAATCTCTATTAGATTGAATTGAAAGATGTTTGTTAAGGCATCGATGCAAGCTATAGATTCTGATACgg          >  1:703281‑M2/9‑150 (MQ=255)

                                                                                                                                              gtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt         >  2:1675677‑M2/8‑150 (MQ=255)

                                                                                                                                               tgctaaATAATAATCAATTGATAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTAAAGGCATGGATGCAAGCTATAGATTCTGATACGGTc        >  2:11460‑M2/7‑150 (MQ=255)

                                                                                                                                                 ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCaa      >  2:2254052‑M2/5‑150 (MQ=255)

                                                                                                                                                 ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGATATAGATTATGATACGGTCaa      >  2:1850732‑M2/5‑150 (MQ=255)

                                                                                                                                                   aaATAATAATCAATTGTTAAATTATTGTGCATTTCACTAGTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATa    <  2:1838095‑M2/148‑1 (MQ=255)

                                                                                                                                                   aaATAATAATCAATTGTTAAATTAATGAGCATTTCACTACTTGAACTGTAATCAGAAAATATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATa    <  1:1011132‑M2/148‑1 (MQ=255)

                                                                                                                                                    aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTCGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTTTAGATTCTGATACGGTCAATaa   >  2:880292‑M2/2‑150 (MQ=255)

AACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAAA  >  NC_000913/1300549‑1300847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`