Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,188,513 G→T G1055V (GGT→GTT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,188,5130GT98.5% 430.9 / NA 137G1055V (GGT→GTT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (64/71);  total (64/73)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

GCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTA  >  NC_000913/4188366‑4188659
                                                                                                                                                   |                                                                                                                                                  
 cGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGttct                                                                                                                                                 <  2:483174/150‑1 (MQ=255)
 cGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGttct                                                                                                                                                 >  2:502635/1‑150 (MQ=255)
    tGGCGAACCGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGttctgtc                                                                                                                                              <  1:401175/150‑1 (MQ=255)
    tGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGttctgtc                                                                                                                                              <  1:1815806/150‑1 (MQ=255)
      gCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCtt                                                                                                                                            <  2:634640/150‑1 (MQ=255)
          aCAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCt                                                                                                                                        >  2:1294510/1‑150 (MQ=255)
             ggTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTAGGCTTCACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCTACGAACTGACCGTTCTGTCTTCGCttgt                                                                                                                                     <  2:1082352/150‑1 (MQ=255)
                tGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTggtggt                                                                                                                                  >  2:1370801/1‑150 (MQ=255)
                tGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTggtggt                                                                                                                                  >  2:753103/1‑150 (MQ=255)
                   tGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTAATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCt                                                                                                                               >  2:1601905/1‑150 (MQ=255)
                    ggcggcGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTg                                                                                                                              <  1:2236438/150‑1 (MQ=255)
                    ggcggcGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGGTATCACCGAAGTAACCGGTTTTGTACGCGTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTg                                                                                                                              <  2:664569/150‑1 (MQ=255)
                     gcggcgAAACCGTTGCAGACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTgg                                                                                                                             <  2:1376034/150‑1 (MQ=255)
                      cggcgAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCGGGTGGTTCTGGa                                                                                                                            >  2:1850909/1‑150 (MQ=255)
                       ggcgAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGAt                                                                                                                           >  1:1012615/1‑150 (MQ=255)
                       ggcgAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGAt                                                                                                                           <  2:1011646/150‑1 (MQ=255)
                         cgAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTc                                                                                                                         <  2:400977/150‑1 (MQ=255)
                          gAAACCGCTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTcc                                                                                                                        <  2:1466694/150‑1 (MQ=255)
                            aaCCGTTGCAAACTGGGACCCGCACACCTTGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGc                                                                                                                      <  2:397965/150‑1 (MQ=255)
                            aaCAGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGATGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGCATTCCGc                                                                                                                      >  1:861591/1‑150 (MQ=255)
                             aCCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCa                                                                                                                     <  1:246252/150‑1 (MQ=255)
                             aCCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCa                                                                                                                     <  2:1822994/150‑1 (MQ=255)
                             aCCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCa                                                                                                                     >  2:1184905/1‑150 (MQ=255)
                               cGTTGCAAACTGGGACCCGCACACAATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAgc                                                                                                                   >  1:272403/1‑149 (MQ=255)
                                   gCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGt                                                                                                               >  1:2036004/1‑150 (MQ=255)
                                    cAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTa                                                                                                              >  1:705389/1‑150 (MQ=255)
                                      aaCTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTAcc                                                                                                            <  2:1607024/150‑1 (MQ=255)
                                        cTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGGCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGc                                                                                                          <  2:244366/150‑1 (MQ=255)
                                         tGGGACCCGCACACCATGCCGGTTATCACCGAAGTAATCTGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCa                                                                                                         <  2:1587312/150‑1 (MQ=255)
                                           ggACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAgg                                                                                                       <  2:1086012/150‑1 (MQ=255)
                                           ggACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAgg                                                                                                       <  1:657496/150‑1 (MQ=255)
                                                cGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGCCCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTa                                                                                                  >  1:1791866/1‑150 (MQ=255)
                                                  cacaCCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTaaa                                                                                                <  2:1424435/150‑1 (MQ=255)
                                                    cacCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGa                                                                                              >  2:203023/1‑150 (MQ=255)
                                                     acCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGAt                                                                                             >  2:1256810/1‑150 (MQ=255)
                                                            cGGTTATCACCGAAGAAAGCGGTTTTGTACGCTTTACTGACATGATAGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGATGGTGGTTCGGGATTCCGCAGAACGTACCGCAGGTGGAAAAGATCTGCGTc                                                                                      >  2:1470865/1‑150 (MQ=255)
                                                             ggTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTcc                                                                                     >  1:272322/1‑150 (MQ=255)
                                                              gTTATCCCCGAAGTAAGCGGTTTTGTACGCTTCACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCg                                                                                    >  1:1574863/1‑150 (MQ=255)
                                                              gTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCg                                                                                    <  1:473982/150‑1 (MQ=255)
                                                              gTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACATATCGCAGGTGGTAAAGATCTGCGTCCg                                                                                    <  1:1850400/150‑1 (MQ=255)
                                                                   cACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACt                                                                               >  2:1162574/1‑150 (MQ=255)
                                                                     ccGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACAGACTAACTGACAGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGa                                                                             >  1:1382514/1‑150 (MQ=255)
                                                                      cGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGaa                                                                            >  1:686639/1‑150 (MQ=255)
                                                                        aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGaaaa                                                                          >  2:373738/1‑150 (MQ=255)
                                                                           tAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTTCGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCg                                                                       <  1:2066251/150‑1 (MQ=255)
                                                                           tAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCg                                                                       <  1:776591/150‑1 (MQ=255)
                                                                             aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGtt                                                                     >  1:515369/1‑150 (MQ=255)
                                                                              gCGGTTTTCTACGCTTTACTGACTTGAGCGACGTCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTg                                                                    <  1:2066281/150‑1 (MQ=255)
                                                                                ggTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGAt                                                                  <  1:1581936/150‑1 (MQ=255)
                                                                                  ttttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGc                                                                <  1:1184905/150‑1 (MQ=255)
                                                                                  ttttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGc                                                                <  1:1270207/150‑1 (MQ=255)
                                                                                    ttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGGCAGCCCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTc                                                              <  2:1390465/150‑1 (MQ=255)
                                                                                     tGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCa                                                             >  2:2135071/1‑150 (MQ=255)
                                                                                      gTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAg                                                            >  2:767565/1‑150 (MQ=255)
                                                                                      gTACGCGTTACTGTCATGCTCTTCGGCCATACCTTTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCGGGTGGTTCTGGATTCCGCAGAACGTACGTCAGGTGGTTAATATATGCGTGGGGCTGTTAATTTCGTTTATGCTCAg                                                            <  2:1048003/150‑1 (MQ=255)
                                                                                       tACGCTTTACTGACATGATGGCCGGCCAGAGCATTACGCGTCAGACCGACGAACTGACGGTTCTGTCTTCGCTGGTGGTTCTTGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAgg                                                           <  1:1787336/150‑1 (MQ=255)
                                                                                       tACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAgg                                                           >  1:751941/1‑150 (MQ=255)
                                                                                        aCGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAggg                                                          <  1:1370801/150‑1 (MQ=255)
                                                                                         cGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGt                                                         >  2:2179394/1‑150 (MQ=255)
                                                                                         cGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGt                                                         <  2:2234623/150‑1 (MQ=255)
                                                                                          gCTTTACTGACATGATCGCCGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTa                                                        <  2:2048774/150‑1 (MQ=255)
                                                                                            tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAac                                                      <  2:546793/150‑1 (MQ=255)
                                                                                            cttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAac                                                      <  1:2223722/149‑1 (MQ=255)
                                                                                             ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGCCGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAATGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                     >  2:827439/1‑150 (MQ=255)
                                                                                              tACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                    <  1:290901/150‑1 (MQ=255)
                                                                                                cTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacgccg                                                  >  1:1949582/1‑150 (MQ=255)
                                                                                                 tGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGt                                                 >  1:916635/1‑150 (MQ=255)
                                                                                                  gACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCAGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGtt                                                >  2:715217/1‑150 (MQ=255)
                                                                                                    cATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCt                                              >  1:1875110/1‑150 (MQ=255)
                                                                                                    cATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTGCTGTCTTCGCTGGTGGTTCTTGTTTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCt                                              <  2:1434026/150‑1 (MQ=255)
                                                                                                      tGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGa                                            >  1:1617450/1‑150 (MQ=255)
                                                                                                      tGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGa                                            <  1:1601905/150‑1 (MQ=255)
                                                                                                         tCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTGCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATcc                                         <  1:2132389/150‑1 (MQ=255)
                                                                                                          cGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATccc                                        <  2:1617450/150‑1 (MQ=255)
                                                                                                           gACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCa                                       <  1:1877376/150‑1 (MQ=255)
                                                                                                             cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGGGGTGCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAgg                                     <  2:576392/150‑1 (MQ=255)
                                                                                                                 cAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTAcc                                 <  1:1216011/150‑1 (MQ=255)
                                                                                                                 cAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTAcc                                 >  1:1396426/1‑150 (MQ=255)
                                                                                                                 cAGACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTAcc                                 <  2:1840295/150‑1 (MQ=255)
                                                                                                                   gACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCGGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                               <  1:1850909/150‑1 (MQ=255)
                                                                                                                   gACCATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                               <  1:1827857/150‑1 (MQ=255)
                                                                                                                    tccATTACGCGTGAGTCCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGGGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGat                              <  2:261471/149‑1 (MQ=255)
                                                                                                                     ccATTACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGata                             >  1:583679/1‑150 (MQ=255)
                                                                                                                        ttACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                          >  2:1227193/1‑150 (MQ=255)
                                                                                                                            gcgTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgc                      <  2:1787943/150‑1 (MQ=255)
                                                                                                                            gcgTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgc                      <  1:1286033/150‑1 (MQ=255)
                                                                                                                             cgTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                     <  1:1156804/150‑1 (MQ=255)
                                                                                                                              gTCAGACCGACGAACTGACCGTTCTGTCTTCGCTTGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcgc                    <  2:83288/150‑1 (MQ=255)
                                                                                                                                  gACCGACGAACTGCCCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACGGATATGGCAGCGCAGTa                >  2:1612265/1‑150 (MQ=255)
                                                                                                                                  gACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTa                <  2:705389/150‑1 (MQ=255)
                                                                                                                                     cgacgaACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACtt             >  1:686497/1‑150 (MQ=255)
                                                                                                                                     cgacgaACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACtt             >  1:1328345/1‑150 (MQ=255)
                                                                                                                                       acgaACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGTTGGTTTTGATCTGGGTCCGGCACTGAATTTCGTTGATGCTCAGGGTAACGACGTGCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTcc           <  1:1013601/150‑1 (MQ=255)
                                                                                                                                        cgaACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCt          >  2:607867/1‑150 (MQ=255)
                                                                                                                                         gaACTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTg         >  1:1541109/1‑150 (MQ=255)
                                                                                                                                          aaCTGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGc        >  1:2153525/1‑150 (MQ=255)
                                                                                                                                            cTGACCGTTCTGGCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCg      <  1:588552/150‑1 (MQ=255)
                                                                                                                                             tGACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCgg     >  1:200063/1‑150 (MQ=255)
                                                                                                                                              gACCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCggg    >  1:1786953/1‑150 (MQ=255)
                                                                                                                                               aCCGTTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGt   <  1:1756997/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGATGGCGAACAGGTTGCTGGCGGCGAAACCGTTGCAAACTGGGACCCGCACACCATGCCGGTTATCACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTA  >  NC_000913/4188366‑4188659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑