Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,716,574 G→T A10S (GCC→TCC)  yiaD → multicopy suppressor of bamB; outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,716,5740GT98.9% 571.0 / NA 181A10S (GCC→TCC) yiaDmulticopy suppressor of bamB; outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (99/80);  total (100/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

CAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGAAGAAAGAT  >  NC_000913/3716428‑3716720
                                                                                                                                                  |                                                                                                                                                  
cAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGcctccg                                                                                                                                                 >  1:1011014/1‑150 (MQ=255)
cAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGcctccg                                                                                                                                                 >  1:2243071/1‑150 (MQ=255)
 aGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGt                                                                                                                                                >  1:1785729/1‑150 (MQ=255)
  gTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTa                                                                                                                                               >  2:1809088/1‑150 (MQ=255)
      aaTATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTagtga                                                                                                                                           >  2:1169003/1‑150 (MQ=255)
      aaTATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTagtga                                                                                                                                           >  2:1012857/1‑150 (MQ=255)
       atatTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTagtgag                                                                                                                                          <  2:2097432/150‑1 (MQ=255)
           tacttacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGgc                                                                                                                                      >  1:1411745/1‑150 (MQ=255)
           tacttacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGgc                                                                                                                                      <  2:9510/150‑1 (MQ=255)
             cttacAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCCCGCGGCATAATCTCCCGCCACGGAACCCGTGGCTAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGgcgc                                                                                                                                    >  2:1305056/1‑150 (MQ=255)
                   aaTTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGTCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGc                                                                                                                              <  2:172106/150‑1 (MQ=255)
                   aaTTTCCGAGTAATCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGc                                                                                                                              <  2:916408/150‑1 (MQ=255)
                       tCCTAGTATTCCCAGGAATAATCTTCATGTTAACGATGCATAATCTCCCTCCACGGAACCCGTGGCAAAGAATAAATAGGGTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGTGTGGCTCTCTGGCGGTa                                                                                                                          >  2:1092445/1‑150 (MQ=255)
                         cGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAAAGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATc                                                                                                                        >  1:655505/1‑150 (MQ=255)
                           aGTATTCCCATGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTg                                                                                                                      <  1:1931529/150‑1 (MQ=255)
                               ttCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTg                                                                                                                  <  1:2189081/150‑1 (MQ=255)
                                 cccAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGATTAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGca                                                                                                                <  1:249884/150‑1 (MQ=255)
                                  ccAGGAATAATCTTCATGTTCACGCGGAATAATCTCGCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGcac                                                                                                               <  2:2018738/150‑1 (MQ=255)
                                     ggAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAAc                                                                                                            <  2:1155783/150‑1 (MQ=255)
                                     ggAATAATCTTCATGTTCACGCGGCATAAGCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTAATGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAAc                                                                                                            <  1:1685401/150‑1 (MQ=255)
                                      gAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACt                                                                                                           >  2:50304/1‑150 (MQ=255)
                                        ataatCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTaa                                                                                                         >  1:748708/1‑150 (MQ=255)
                                        ataatCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTaa                                                                                                         >  1:2065626/1‑150 (MQ=255)
                                         taatCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAAc                                                                                                        <  1:1904647/150‑1 (MQ=255)
                                           atCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAAccc                                                                                                      <  2:577082/150‑1 (MQ=255)
                                            tCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCt                                                                                                     <  2:958147/150‑1 (MQ=255)
                                                      cACGCGGCATAATCTCCCGCCACTGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATATTATTGCCTCCGTAGTGAGTGGCTCTATGTCGGTATCTGGCTGCACAACTAACCCTTACACCGGCg                                                                                           >  2:2014527/1‑150 (MQ=255)
                                                      cACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCCGAACTAACCCTTACACCGGCg                                                                                           >  1:223227/1‑150 (MQ=255)
                                                      cACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGGTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCg                                                                                           >  2:381580/1‑150 (MQ=255)
                                                          cgGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTTCACAACTAACCCTTACACCGGCGAAcg                                                                                       <  2:1450755/150‑1 (MQ=255)
                                                          cgGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACCCCGGCGAAcc                                                                                       >  2:354413/1‑149 (MQ=255)
                                                          cgGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAAcg                                                                                       <  1:1406436/150‑1 (MQ=255)
                                                           ggCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAAcgc                                                                                      <  2:675432/150‑1 (MQ=255)
                                                             cATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGa                                                                                    >  1:2126192/1‑150 (MQ=255)
                                                              aTAATCTCCAGCCACGGAACCCGTGGCAAAGAATAAAAAGTTTAGTAAGGATTAACAATGAAGAAACGTGATGATCTTATTGCCTCCGTAGTGAGTGGAGCTCTGGCGTTATCTGTCTGCAAAACTAAACATTAAAACGGCGAACTCGaa                                                                                   >  2:1843722/1‑150 (MQ=255)
                                                               tAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAg                                                                                  <  2:1059923/150‑1 (MQ=255)
                                                               tAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAg                                                                                  >  1:853812/1‑150 (MQ=255)
                                                               tAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAg                                                                                  >  1:1843532/1‑150 (MQ=255)
                                                               tAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAg                                                                                  >  1:1113602/1‑150 (MQ=255)
                                                                 aTCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCa                                                                                >  2:1262241/1‑150 (MQ=255)
                                                                    tcCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGt                                                                             >  2:466493/1‑150 (MQ=255)
                                                                    tcCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCATCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGt                                                                             >  1:937483/1‑150 (MQ=255)
                                                                    ctttgCCACGGGTTCCGTGGCGAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGt                                                                             >  1:454292/5‑150 (MQ=255)
                                                                     tttgCCACGGGTTCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTa                                                                            >  1:1871969/4‑150 (MQ=255)
                                                                     cccGCCACGGAACCTGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTa                                                                            <  1:1768254/150‑1 (MQ=255)
                                                                      ccGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTaa                                                                           >  1:801502/1‑150 (MQ=255)
                                                                         ccACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATc                                                                        >  2:2143457/1‑150 (MQ=255)
                                                                          cACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCt                                                                       <  1:1086777/150‑1 (MQ=255)
                                                                          cACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCt                                                                       <  1:1033195/150‑1 (MQ=255)
                                                                                aCCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATc                                                                 <  2:1859485/150‑1 (MQ=255)
                                                                                 cccGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTACCCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCg                                                                >  2:1387073/1‑150 (MQ=255)
                                                                                  ccGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCgg                                                               >  2:1484932/1‑150 (MQ=255)
                                                                                  ccGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCATTACACCGGCGAAAGCGAAGCAGGTAAATCTGCTATCgg                                                               >  1:528922/1‑150 (MQ=255)
                                                                                       gCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAg                                                          >  1:263322/1‑150 (MQ=255)
                                                                                        cAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAgg                                                         >  2:393081/1‑150 (MQ=255)
                                                                                        cAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAgg                                                         >  1:714645/1‑150 (MQ=255)
                                                                                          aaGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTc                                                       <  2:2186187/150‑1 (MQ=255)
                                                                                             aaTAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTgg                                                    >  2:722204/1‑150 (MQ=255)
                                                                                                aaaaaGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGct                                                 >  2:485190/1‑150 (MQ=255)
                                                                                                  aaaGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGctct                                               >  2:1650726/1‑150 (MQ=255)
                                                                                                    aGGTTATTAAGGATAAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGctctct                                             <  2:429228/150‑1 (MQ=255)
                                                                                                      gTTATTATGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCg                                           <  1:1238901/150‑1 (MQ=255)
                                                                                                      gTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCg                                           >  1:1609911/1‑150 (MQ=255)
                                                                                                        tattaAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTg                                         <  1:2107028/150‑1 (MQ=255)
                                                                                                         attaAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTgg                                        <  1:1809088/150‑1 (MQ=255)
                                                                                                         attaAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTgg                                        >  2:1918006/1‑150 (MQ=255)
                                                                                                         ataaaGCATTAACAATGAAGAAACGTGTTTATCTAATTGCCTCCGTAGTGAGGGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCGCTCTCGTgg                                        <  1:1689276/147‑1 (MQ=255)
                                                                                                          ttaAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTTGTggg                                       <  1:622599/150‑1 (MQ=255)
                                                                                                          ttaAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTggg                                       <  2:618137/150‑1 (MQ=255)
                                                                                                           taAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCATTCGGCCCAGGTCTGGGCTCTCACGTGGgc                                      >  1:1541460/1‑150 (MQ=255)
                                                                                                                   aaCAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTAt                              <  2:1196006/150‑1 (MQ=255)
                                                                                                                   aaCAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTAt                              <  2:1188226/150‑1 (MQ=255)
                                                                                                                   aaCAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTAt                              >  2:1370535/1‑150 (MQ=255)
                                                                                                                     cAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACATCTAACCCGTACACCGGCGCACGCGAAGCAGGTAAATCTGATATCGGCGCAGGTCTGGGCGCTCTCGTGGGCGCGGGGATTg                            >  2:165717/1‑150 (MQ=255)
                                                                                                                     cAATGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTg                            >  2:412627/1‑150 (MQ=255)
                                                                                                                      aaTGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTgg                           <  2:2243071/150‑1 (MQ=255)
                                                                                                                      aaTGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTgg                           <  1:1472326/150‑1 (MQ=255)
                                                                                                                      aaTGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTgg                           <  1:1008564/150‑1 (MQ=255)
                                                                                                                       aTGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCTGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGt                          >  1:32281/1‑150 (MQ=255)
                                                                                                                       aTGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGt                          >  2:1038329/1‑150 (MQ=255)
                                                                                                                       aTGAAGAAACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGt                          >  2:1406608/1‑150 (MQ=255)
                                                                                                                         gaagaaACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCTCAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCGCGTGGGCGCGGGTATTGGTgc                        >  1:597931/1‑150 (MQ=255)
                                                                                                                          aagatACGTGTTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACGCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTTGGCTCTCTCGTGGGCGCGGGTATTGGTgcg                       <  1:1644466/150‑1 (MQ=255)
                                                                                                                           agtaaCGTGTTTATCTTATGGCCTCCGGAGTGCGTGGCGCTCTGGCGGCATCTGGCTGCCCAACAAACCCTTACACCGGCGAACGCGAAGCAGGGAAATCTGCTAGCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTgcgc                      <  1:355035/147‑1 (MQ=255)
                                                                                                                                cGTGCTTATCTTAATCCCTCCGTCGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGctctct                 <  2:818739/150‑1 (MQ=255)
                                                                                                                                 gtgtTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCtctt                >  1:69649/1‑150 (MQ=255)
                                                                                                                                 gtgtTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCtctt                <  1:2143457/150‑1 (MQ=255)
                                                                                                                                  tgtTTATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCtcttc               >  2:404232/1‑150 (MQ=255)
                                                                                                                                     ttATCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCttcttc            >  2:169372/1‑150 (MQ=255)
                                                                                                                                      tagcTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCg           <  2:1594322/147‑1 (MQ=255)
                                                                                                                                       aTCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCga          >  1:2004698/1‑150 (MQ=255)
                                                                                                                                        tCTTATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCgaa         <  1:4169/150‑1 (MQ=255)
                                                                                                                                          ttATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGGATCTGGCTGCACAACTACCCCTTCCACCGGCGAACGCGACGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCgaaga       <  1:1495039/150‑1 (MQ=255)
                                                                                                                                           tATTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCgaagaa      >  2:1768702/1‑150 (MQ=255)
                                                                                                                                            aTTGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGaagcaa     >  1:2031509/1‑150 (MQ=255)
                                                                                                                                             ttGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGaagaaag    <  2:540276/150‑1 (MQ=255)
                                                                                                                                             ttGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGaagaaag    <  1:2137901/150‑1 (MQ=255)
                                                                                                                                              tGCCTCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTACCCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGaagaaaga   >  2:1639385/1‑150 (MQ=255)
                                                                                                                                               gcctccGTAGTGAGTGGCGCTCTGTCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGAAGAAAGAt  >  2:2132196/1‑150 (MQ=255)
                                                                                                                                               gcctccGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGAAGAATGAt  >  1:1505947/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CAGTTAAATATTACTTACAAATTTCCGAGTATTCCCAGGAATAATCTTCATGTTCACGCGGCATAATCTCCCGCCACGGAACCCGTGGCAAAGAATAAAAAGGTTATTAAGGATTAACAATGAAGAAACGTGTTTATCTTATTGCCGCCGTAGTGAGTGGCGCTCTGGCGGTATCTGGCTGCACAACTAACCCTTACACCGGCGAACGCGAAGCAGGTAAATCTGCTATCGGCGCAGGTCTGGGCTCTCTCGTGGGCGCGGGTATTGGTGCGCTCTCTTCTTCGAAGAAAGAT  >  NC_000913/3716428‑3716720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑