Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,263,308 C→G 5.1% Q308H (CAG→CAC qorA ← quinone oxidoreductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,263,3080CG 5.1% 1027.0 / 35.8 275Q308H (CAG→CACqorAquinone oxidoreductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base C (144/117);  new base G (7/7);  total (151/124)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 275 total aligned reads displayed.

GCGCCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTCACATACA  >  NC_000913/4263161‑4263456
                                                                                                                                                   |                                                                                                                                                    
gcgcCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTg                                                                                                                                                    >  1:1769040/1‑150 (MQ=255)
   ccTCTAGCAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTgcgc                                                                                                                                                 <  2:2252984/150‑1 (MQ=255)
     tctAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCAt                                                                                                                                               <  2:3397290/150‑1 (MQ=255)
     tctAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCAt                                                                                                                                               >  1:1781806/1‑150 (MQ=255)
      ctAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCAGCGTGCGCAtg                                                                                                                                              <  2:1911314/150‑2 (MQ=255)
      ctAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCAGCGTGCGCAtg                                                                                                                                              <  2:1362757/150‑2 (MQ=255)
       tAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATcc                                                                                                                                             >  1:3827248/1‑150 (MQ=255)
         aCAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCtt                                                                                                                                           >  1:3032507/1‑150 (MQ=255)
         aCAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCtt                                                                                                                                           <  2:3434532/150‑1 (MQ=255)
           aGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCa                                                                                                                                         >  1:2097379/1‑150 (MQ=255)
              ttCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCg                                                                                                                                      >  2:518896/1‑150 (MQ=255)
              ttCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCg                                                                                                                                      >  2:3289702/1‑150 (MQ=255)
               tCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCgg                                                                                                                                     >  2:2721551/1‑150 (MQ=255)
               tCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCgg                                                                                                                                     >  1:2627706/1‑150 (MQ=255)
                     tGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATAttt                                                                                                                               <  1:3406213/150‑1 (MQ=255)
                       cTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTct                                                                                                                             <  1:2961146/150‑1 (MQ=255)
                        tGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTTCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTctg                                                                                                                            <  2:715547/150‑1 (MQ=255)
                            cccTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGACTCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCtgctgc                                                                                                                        <  2:819046/150‑1 (MQ=255)
                              cTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTc                                                                                                                      >  1:1411380/1‑150 (MQ=255)
                                 catacaGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGc                                                                                                                   <  2:2051775/150‑1 (MQ=255)
                                    acaGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGAc                                                                                                                <  2:2568678/150‑1 (MQ=255)
                                      aGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACAt                                                                                                              <  1:3611670/150‑1 (MQ=255)
                                      aGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACAt                                                                                                              >  1:1241516/1‑150 (MQ=255)
                                        ccGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGTAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCg                                                                                                            >  2:593700/1‑150 (MQ=255)
                                         cGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGa                                                                                                           <  1:423825/150‑1 (MQ=255)
                                          gAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGAc                                                                                                          <  2:1478488/150‑1 (MQ=255)
                                                taAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAAt                                                                                                    >  1:1635832/1‑150 (MQ=255)
                                                taAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAAt                                                                                                    >  1:692986/1‑150 (MQ=255)
                                                taAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAAt                                                                                                    >  2:1628064/1‑150 (MQ=255)
                                                taAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATGTCTTTTATGGACTCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAAt                                                                                                    >  2:1627929/1‑150 (MQ=255)
                                                 aaaaaGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATc                                                                                                   >  2:3216137/1‑150 (MQ=255)
                                                  aaaaGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATca                                                                                                  >  2:2267922/1‑150 (MQ=255)
                                                      gaaagGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACAcc                                                                                              >  1:926602/1‑150 (MQ=255)
                                                       aaagGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCg                                                                                             >  2:3900715/1‑150 (MQ=255)
                                                       aaagGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCg                                                                                             >  2:966/1‑150 (MQ=255)
                                                         agGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCt                                                                                           <  2:1134030/150‑1 (MQ=255)
                                                         agGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCt                                                                                           <  2:497364/150‑1 (MQ=255)
                                                          gggCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTg                                                                                          >  2:3698001/1‑150 (MQ=255)
                                                           ggCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGACTCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTgg                                                                                         >  2:1220940/1‑150 (MQ=255)
                                                           ggCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTgg                                                                                         <  1:1905070/150‑1 (MQ=255)
                                                                cccAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATc                                                                                    >  1:90060/1‑150 (MQ=255)
                                                                cccAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATc                                                                                    <  1:167736/150‑1 (MQ=255)
                                                                cccAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATc                                                                                    >  1:3248529/1‑150 (MQ=255)
                                                                 ccAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCa                                                                                   >  1:2848252/1‑150 (MQ=255)
                                                                   aGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCaaa                                                                                 >  2:2320396/1‑150 (MQ=255)
                                                                   aGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCaaa                                                                                 >  1:294989/1‑150 (MQ=255)
                                                                   aGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCaaa                                                                                 >  2:315577/1‑150 (MQ=255)
                                                                   aGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCaaa                                                                                 <  1:3286841/150‑1 (MQ=255)
                                                                        gAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGaa                                                                            <  2:1335441/150‑1 (MQ=255)
                                                                          aGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACa                                                                          >  2:3351429/1‑150 (MQ=255)
                                                                          aGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACa                                                                          <  1:2596302/150‑1 (MQ=255)
                                                                            cccTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGt                                                                        >  2:2593322/1‑150 (MQ=255)
                                                                             ccTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGtt                                                                       >  2:2875311/1‑150 (MQ=255)
                                                                              cTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTc                                                                      >  2:896564/1‑150 (MQ=255)
                                                                              cTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTc                                                                      >  2:1977144/1‑150 (MQ=255)
                                                                               tATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCa                                                                     >  2:3242638/1‑150 (MQ=255)
                                                                               tATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCa                                                                     <  2:306851/150‑1 (MQ=255)
                                                                                aTTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCAt                                                                    <  1:98469/150‑1 (MQ=255)
                                                                                 tttCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCAtt                                                                   >  1:2370537/1‑150 (MQ=255)
                                                                                 tttCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCAtt                                                                   >  1:1548867/1‑150 (MQ=255)
                                                                                    cTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACt                                                                <  1:2573049/150‑1 (MQ=255)
                                                                                    cTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACt                                                                <  2:274542/150‑1 (MQ=255)
                                                                                     ttttATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTg                                                               >  1:2261696/1‑150 (MQ=255)
                                                                                     ttttATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTg                                                               <  2:2097379/150‑1 (MQ=255)
                                                                                       ttATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGc                                                             <  2:3635292/150‑1 (MQ=255)
                                                                                         aTGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCt                                                           >  2:384446/1‑150 (MQ=255)
                                                                                         aTGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCt                                                           >  2:1367312/1‑150 (MQ=255)
                                                                                         aTGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCt                                                           <  1:1021782/150‑1 (MQ=255)
                                                                                                cagcagGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTaa                                                    <  2:2722905/150‑1 (MQ=255)
                                                                                                 agcagGCTGGAACCTTGCGTCGCCCGGCTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAAtt                                                  >  2:2487823/1‑150 (MQ=255)
                                                                                                  gcagGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAAtt                                                  >  1:2230733/1‑150 (MQ=255)
                                                                                                   cagGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATtc                                                 <  1:2809756/150‑1 (MQ=255)
                                                                                                             ccTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCgtg                                       <  2:2897647/150‑1 (MQ=255)
                                                                                                               ttGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCgtggt                                     >  1:3356878/1‑150 (MQ=255)
                                                                                                                 gCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGa                                   <  2:1769040/150‑1 (MQ=255)
                                                                                                                   gTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGata                                 >  2:2792876/1‑150 (MQ=255)
                                                                                                                          ggCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCtt                          >  1:3511608/1‑150 (MQ=255)
                                                                                                                          ggCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCtt                          >  1:773161/1‑150 (MQ=255)
                                                                                                                             tttCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCa                       <  1:1007011/150‑1 (MQ=255)
                                                                                                                               tCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAgg                     >  1:3100187/1‑150 (MQ=255)
                                                                                                                                ccAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAggg                    >  1:3021625/1‑150 (MQ=255)
                                                                                                                                ccAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAggg                    >  1:793058/1‑150 (MQ=255)
                                                                                                                                 cAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGa                   >  2:1051951/1‑150 (MQ=255)
                                                                                                                                  aGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGaa                  >  1:1498924/1‑150 (MQ=255)
                                                                                                                                  aGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGaa                  <  2:2128405/150‑1 (MQ=255)
                                                                                                                                    aaTCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAgg                <  1:610638/150‑1 (MQ=255)
                                                                                                                                    aaTCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAgg                >  1:2345601/1‑150 (MQ=255)
                                                                                                                                        tcATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCgt            >  2:1534686/1‑150 (MQ=255)
                                                                                                                                        ggaTGCGCAGCGTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCgt            >  1:3163544/3‑150 (MQ=255)
                                                                                                                                        ggaTGCGCAGCGTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCgt            >  1:855109/3‑150 (MQ=255)
                                                                                                                                         gaTGCGCAGCGTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCgtg           >  1:270548/2‑150 (MQ=255)
                                                                                                                                          aTGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCgtgt          <  2:1921421/150‑1 (MQ=255)
                                                                                                                                          aTGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCgtgt          >  1:971215/1‑150 (MQ=255)
                                                                                                                                          aTGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCgtgt          >  1:3562567/1‑150 (MQ=255)
                                                                                                                                          aTGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCgtgt          <  2:1679146/150‑1 (MQ=255)
                                                                                                                                           tGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTc         <  1:548360/150‑1 (MQ=255)
                                                                                                                                             cgcACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTACTCACCCCGCTGGCAATCCAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTcac       <  2:3765987/150‑1 (MQ=255)
                                                                                                                                                aCGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTCACATa    <  2:482577/150‑1 (MQ=255)
                                                                                                                                                 cGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTCACATAc   <  2:3165197/150‑1 (MQ=255)
                                                                                                                                                  gCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTCACATACa  >  2:1118676/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCGCCTCTAACAGATTCATCGTGCTGTACCCTACATACAGCCGAACTATAAAAAGAAAGGGCTTCCCAGGTGGAAGCCCTATTTCTTTTATGGAATCAGCAGGCTGGAACCTTGCGTCGCCCGGCTTTCCAGAATCTCATGCGCACGCTGCGCATCCTTCAGCGGATATTTCTGCTGCTCGGCGACATCGACCTTAATCACACCGCTGGCAATCAAAGAGAACAGTTCATTACTGGCCTCGGTTAATTCCTCCCGCGTGGTGATATAGCCTTGCAGGGAAGGGCGTGTCACATACA  >  NC_000913/4263161‑4263456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: