Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 349,673 G→C 5.3% intergenic (+101/‑339) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913349,6730GC 5.3% 829.1 / 15.6 247intergenic (+101/‑339)prpB/prpC2‑methylisocitrate lyase/2‑methylcitrate synthase
Reads supporting (aligned to +/- strand):  ref base G (86/145);  new base C (14/2);  total (100/147)
Fisher's exact test for biased strand distribution p-value = 9.18e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

ACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGC  >  NC_000913/349526‑349822
                                                                                                                                                   |                                                                                                                                                     
aCGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGAt                                                                                                                                                     <  1:2638046/150‑1 (MQ=255)
aCGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGAt                                                                                                                                                     <  2:47150/150‑1 (MQ=255)
aCGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGAt                                                                                                                                                     <  1:3776702/150‑1 (MQ=255)
       gaAGCTCGCCACCCTGTTGGCCCGTAGCCCGGTGAAATAAAACACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCg                                                                                                                                              <  2:3672941/150‑1 (MQ=25)
          gCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCAt                                                                                                                                           <  2:1742638/150‑1 (MQ=255)
              gACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAACACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGa                                                                                                                                       <  1:810160/150‑1 (MQ=255)
              gACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGa                                                                                                                                       <  1:534237/150‑1 (MQ=255)
              gACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                                                                                                                       >  2:2446067/1‑150 (MQ=255)
              gACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                                                                                                                       >  1:3246713/1‑150 (MQ=255)
                 aaCCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACaa                                                                                                                                    >  2:1665224/1‑150 (MQ=255)
                   ccggtgggCCCGTCGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAAcc                                                                                                                                  <  1:3051051/143‑1 (MQ=255)
                    cTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCAGATGCGACGCTGACGCGACTTAACAGGCCTACGAGGTGCACATAACAGTAGGTCTCATAATACGGATGGCGTCGCATCCGACAACCg                                                                                                                                 >  1:301640/1‑150 (MQ=255)
                     tGTTTGCCCGTTGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGa                                                                                                                                <  2:2073574/150‑1 (MQ=255)
                      gTTTGCCCGTAGCCAGGTTAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGAt                                                                                                                               <  1:1893915/150‑1 (MQ=255)
                                 gCCAGGTGAAATAAAAAACGCCCGTGGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATgcgc                                                                                                                    >  1:1278316/1‑150 (MQ=255)
                                    cggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                                                                                                                 <  1:1671070/149‑1 (MQ=255)
                                     ggTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCa                                                                                                                                            <  2:781445/122‑1 (MQ=255)
                                      gTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTg                                                                                                               <  1:3338703/150‑1 (MQ=255)
                                      gTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTg                                                                                                               <  1:2736826/150‑1 (MQ=255)
                                       tGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGa                                                                                                              <  2:914980/150‑1 (MQ=255)
                                          aaTAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGt                                                                                                           <  1:538813/150‑1 (MQ=255)
                                            tAAAAAACGCCCGTTGATTGTATTCGACAGCCGATTCCTGAAGCGTCGCTGACGCGACTTATAAGGCCAACGAGGTGCAATGAACTGTAGGTCGGATAATACCGATTGCGTCGCATCCGACAAACGATGCCTGATTCGCCGCTGAAGTGa                                                                                                         >  1:2191253/1‑150 (MQ=18)
                                            tAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGa                                                                                                         <  2:848861/150‑1 (MQ=255)
                                            tAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGa                                                                                                         <  1:2837664/150‑1 (MQ=255)
                                             aaaaaaCGCCCGTTGATTGTATTCGACAGCCGATTCCAGATTCGTCGCTGACGCGACATATAAGGCCTACGAGGTGCACTGAACTTTAGGTCGGATAAGACGGATTGCGTCGCATACGACAACCGAAGCCAGATGCGCCGCTGACGTGaa                                                                                                        >  1:15331/1‑149 (MQ=255)
                                             aaaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGAc                                                                                                        <  1:3836522/150‑1 (MQ=255)
                                             aaaaaaCGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACATATCAGGCCTACGGGGTGCACAGAAAAGTAGGTCGGATAATACGCATAGCGTCGCATCCGACAACCGAATCCATAAGCGCCCCTGACgagaa                                                                                                        >  1:3296242/1‑149 (MQ=14)
                                               aaatCGGCCTTTGTTTGTATTCGACAGCCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGGATCGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACtt                                                                                                      <  2:301682/150‑1 (MQ=11)
                                                     cccGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAgg                                                                                                <  2:1247822/150‑1 (MQ=255)
                                                           gactGTATTCGCCAGCCGAGGCCTGATGCGTCGCTGACGCGACGTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACg                                                                                          <  2:2503391/147‑1 (MQ=255)
                                                             ttGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                        <  1:2321956/150‑1 (MQ=255)
                                                             ttGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                        <  1:634559/150‑1 (MQ=255)
                                                             ttGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACggg                                                                                        <  1:1376010/150‑1 (MQ=255)
                                                                tATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTg                                                                                     <  1:39499/150‑1 (MQ=255)
                                                                    cGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACt                                                                                 <  1:1613393/150‑1 (MQ=255)
                                                                     gACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTg                                                                                <  2:235634/150‑1 (MQ=255)
                                                                      aCAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACGTATCAGGCCTACGGGGTGCACTGa                                                                               >  2:3330261/1‑150 (MQ=255)
                                                                       cAGCCGATGCCTGATGCGTCGCTGATGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                                                                              <  1:3577194/150‑1 (MQ=255)
                                                                       cAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGaa                                                                              <  1:19760/150‑1 (MQ=255)
                                                                        aGCCGATGCCTGATGCGTCGCAGAAGCGACTTATCACGCCTACGAGGTGCACAGAACAGTAGGTCGGATAAGACGGATTGCGTCGCAACCGACAACCCAATCCAGAAGCCCCCCAGACCAGACTAATCAGGCCAAAGGGGTGAACAGAAc                                                                             >  1:2476342/1‑150 (MQ=255)
                                                                         tccGATGCCTGATGCGTCGCTGACTCGACTTATCAGGCCTACGCGGTGCACTGAACTGGAGGTCGGGTAAGACGGATGGCGTCGAATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACt                                                                            <  2:1972640/149‑1 (MQ=14)
                                                                         gCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGAAGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTCCGGGGTGCACGGAACt                                                                            >  2:2776/1‑150 (MQ=255)
                                                                            gATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTa                                                                         <  1:1152110/150‑1 (MQ=255)
                                                                                 cTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGTACTGTAGGTCGGATAAGACGGCTGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTCCGGGGGGCCCTGTCCTGGAGGTCg                                                                    >  1:2162466/1‑150 (MQ=14)
                                                                                 cTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg                                                                    <  1:460411/150‑1 (MQ=255)
                                                                                 cTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg                                                                    <  1:722270/150‑1 (MQ=255)
                                                                                 cTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCg                                                                    <  2:2933685/150‑1 (MQ=255)
                                                                                  tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg                                                                   >  2:3418645/1‑150 (MQ=255)
                                                                                  tGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCgg                                                                   >  1:2150290/1‑150 (MQ=255)
                                                                                      gCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATaa                                                               <  1:824702/150‑1 (MQ=255)
                                                                                        gTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATTCCTGATGAGACGCTGACGGGACTTATCAGGCATACGGGGTGAACTGAGCTGTAGGTCGGATATGa                                                             >  2:814897/1‑150 (MQ=11)
                                                                                        gTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa                                                             <  1:227216/150‑1 (MQ=255)
                                                                                        gTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa                                                             <  1:2217295/150‑1 (MQ=255)
                                                                                        gTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGa                                                             <  2:2600142/150‑1 (MQ=255)
                                                                                           gCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGc                                                          <  1:3102263/150‑1 (MQ=255)
                                                                                               aCGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAg                                                      >  2:1628239/1‑150 (MQ=255)
                                                                                               aCGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATCGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACggg                                                                                        >  1:1799032/1‑116 (MQ=12)
                                                                                                cgcgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGc                                                     <  1:1143603/150‑1 (MQ=255)
                                                                                                 gcgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCg                                                    >  2:2726948/1‑150 (MQ=40)
                                                                                                 gcgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGAGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCg                                                    >  1:3203382/1‑150 (MQ=21)
                                                                                                  cgACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGt                                                   >  1:2523293/1‑150 (MQ=40)
                                                                                                   gACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTc                                                  <  2:592933/150‑1 (MQ=39)
                                                                                                   gACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGACGCTGAAGAGACTTATCAGGCCTACGGGGTGAAATGAACTGAATGACGGATAAGACGCATAGCGTc                                                  >  2:3749282/1‑150 (MQ=11)
                                                                                                    aCTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACgg                                                                                                                                                       >  1:2487049/1‑48 (MQ=255)
                                                                                                    aCTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCTGATAAGACGAATTGCGTCg                                                 >  2:2093369/1‑150 (MQ=255)
                                                                                                     cTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGc                                                >  1:1261999/1‑150 (MQ=39)
                                                                                                     cTTATCAGGCCTACGAGGGGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGc                                                <  2:3820850/150‑1 (MQ=255)
                                                                                                        aTCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATc                                             <  1:761196/150‑1 (MQ=17)
                                                                                                           aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                          >  1:222219/1‑150 (MQ=39)
                                                                                                           aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                          <  2:2554267/150‑1 (MQ=39)
                                                                                                           aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATcc                                            >  1:837555/1‑148 (MQ=17)
                                                                                                           aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGCCTACGGGGTGAACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATcc                                            <  2:837555/148‑1 (MQ=17)
                                                                                                           aGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCACATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGa                                          >  1:2891655/1‑150 (MQ=255)
                                                                                                             gCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACa                                        <  2:188050/150‑1 (MQ=39)
                                                                                                                    aGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGCTGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATg                                 <  2:2904783/150‑1 (MQ=38)
                                                                                                                       tGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCt                              <  2:3232934/150‑1 (MQ=37)
                                                                                                                         cACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGa                            <  2:2191253/150‑1 (MQ=37)
                                                                                                                         cACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGa                            >  1:2812037/1‑150 (MQ=37)
                                                                                                                           cTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATg                          <  1:2071913/150‑1 (MQ=37)
                                                                                                                            tGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGa                            >  2:3934865/1‑147 (MQ=37)
                                                                                                                                cTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCTTAGCGTCGCATCCGACAACCGATGCCTGATGcgccg                     >  1:1006622/1‑150 (MQ=37)
                                                                                                                                 tGTAGGTCTGATAAGACGGAGGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGGATTGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                    <  2:541252/150‑1 (MQ=255)
                                                                                                                                 tGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                    <  1:2581036/150‑1 (MQ=37)
                                                                                                                                 tGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGcgccgc                    >  1:3209894/1‑150 (MQ=37)
                                                                                                                                 tGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACAGATGCCTGATGcgccgc                    >  2:3590186/1‑150 (MQ=37)
                                                                                                                                  gTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCt                   >  1:1038833/1‑150 (MQ=37)
                                                                                                                                     ggTCGGATAAGACGGATGGCGTCGCATCCGACACCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAc                <  2:1725035/150‑1 (MQ=37)
                                                                                                                                        cGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGAcgcg             >  1:2363299/1‑150 (MQ=37)
                                                                                                                                         ggATAAGACGGATGGCGTCGCCTCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGa            <  2:1852795/150‑1 (MQ=37)
                                                                                                                                         ggATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGa            <  2:1174907/150‑1 (MQ=37)
                                                                                                                                         ggATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGa            <  2:623068/150‑1 (MQ=37)
                                                                                                                                         ggATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGACGCTGACGTGACTTATCAGGCCTACGTGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGa            <  2:1529756/150‑1 (MQ=255)
                                                                                                                                           aTAAGACGGCTGGCGTCGCCTCCGACACCCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACt          <  2:2483910/150‑1 (MQ=255)
                                                                                                                                           aTAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACt          <  2:2483943/150‑1 (MQ=37)
                                                                                                                                              aGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTAt       <  2:1021398/150‑1 (MQ=37)
                                                                                                                                                aCGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCa     >  1:3872746/1‑150 (MQ=37)
                                                                                                                                                  ggATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg   <  1:3418645/150‑1 (MQ=35)
                                                                                                                                                  ggATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAgg   >  1:3116795/1‑150 (MQ=35)
                                                                                                                                                   gATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGc  <  1:1693716/150‑1 (MQ=35)
                                                                                                                                                   gATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGc  <  1:3532721/150‑1 (MQ=35)
                                                                                                                                                   |                                                                                                                                                     
ACGAAGAGAAGCTCGACAACCTGTTTGCCCGTAGCCAGGTGAAATAAAAAACGCCCGTTGATTGTATTCGACAGCCGATGCCTGATGCGTCGCTGACGCGACTTATCAGGCCTACGAGGTGCACTGAACTGTAGGTCGGATAAGACGGATGGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAGGCCTACGGGGTGCACTGAACTGTAGGTCGGATAAGACGCATAGCGTCGCATCCGACAACCGATGCCTGATGCGCCGCTGACGCGACTTATCAGGC  >  NC_000913/349526‑349822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: