Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,004 A→C 8.4% intergenic (‑123/+65) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0040AC 8.4% 473.9 / 5.7 165intergenic (‑123/+65)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (115/30);  new base C (4/13);  total (120/45)
Fisher's exact test for biased strand distribution p-value = 7.29e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCC  >  NC_000913/379856‑380150
                                                                                                                                                    |                                                                                                                                                    
ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                                                                                                     >  1:626005/1‑150 (MQ=255)
ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                                                                                                     <  2:3016571/150‑1 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAagag                                                                                                                                                  >  2:1241845/1‑150 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAagag                                                                                                                                                  <  2:2379575/150‑1 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGGCGGACAACACagag                                                                                                                                                  >  1:1720665/1‑150 (MQ=255)
       cttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaaa                                                                                                                                             <  2:2887657/150‑1 (MQ=255)
             gAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                                                                                                                      >  1:1221270/1‑150 (MQ=255)
             gAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                                                                                                                      >  1:1689736/1‑150 (MQ=255)
                 aCTGGGCATTTCGCACATAATCATCTGCATGAATTATACTATTGGGGGTTATTCTATATGTAAATGAATACCCCCCTATATTATATAACACTGGAGAATAAATTTTATCCGGTGATTGTGGTCGTAAAAAAAACAGGAAAgggggggggg                                                                                                                                  <  2:1431849/150‑1 (MQ=255)
                  cTGGGCATTTCTGAACTCTTCATGTTCATGCAATATACTTTAGGGGGGTATTCTATATGTCTATTAATACCCCCCTATATTATATAACACTTTTGAATAAAATTTATCCGGTGAATGTTGTCGGAAAACAAAGAGGAAAGGGGGGGGGGc                                                                                                                                 <  2:2979920/150‑1 (MQ=255)
                    gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTAGTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggctaa                                                                                                                               >  1:3821252/1‑146 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggggcta                                                                                                                              >  2:2818526/1‑146 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggggcta                                                                                                                              >  2:1040914/1‑146 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTaa                                                                                                                              >  1:2619322/1‑150 (MQ=255)
                          ttCGCACCTAATCATCTTCATGAATTATACTATTGGGGGTTATTCTATTTTTCAATTCATACCCCCCTTTATTATATAACACTGTATAATAAAATTTATCCGGTGTATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGc                                                                                                                         <  2:1763040/150‑1 (MQ=255)
                           tctcACCTCATCATCTGCTTGTCATATACTCGAGGGGGGTTTTCTATATGTCATTGCATACCCCCCTTTAGTATATAACCCTGGAGAATACAATTTATCCGGGGATTGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCa                                                                                                                        <  2:3347751/147‑1 (MQ=255)
                           tCGCCCCTCCTCATCTGCATGCAAATTACTCTCGGGGTGTATTATATATTTCAAGGCATACCCCCCTATTGTATATAACCCGGGCGAATAAAATTTAGCCGGGGAAGGTGGCCGGAAAACAATCAGCAACGGGGGGGGGGCTAATCGGCa                                                                                                                        <  2:3635437/150‑1 (MQ=255)
                           tCGCACCTCATCTTCTGCATGCAATAGACTATTGGGGGGTTTTCTATATTTCACTGCATACCCCCGTATAGTATATCCCACTGGAGAATAAAATTTAGCCGGTGAAGGGGGTCGGAAAACACCGAGGAAAGGGGGGGGGGCTAATCGGCa                                                                                                                        <  2:2673682/150‑1 (MQ=255)
                           tCGCACCTCATCATCTGCATGCAATATACTCTCGGGGGTTATTCTATATGTCAATGCATACCCCCCTTTAGTATATAACACTGGAGAATAAACTTTATCCGGTGAATGTGGTCGGAAAACAACGAGGAAAGGGGGGGGGGCTAATCGGCa                                                                                                                        <  2:3773305/150‑1 (MQ=255)
                           tCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATTGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCa                                                                                                                        <  2:666749/150‑1 (MQ=255)
                             gCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAgg                                                                                                                      >  1:346070/1‑150 (MQ=255)
                             gCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAgg                                                                                                                      >  1:1074046/1‑150 (MQ=255)
                              caactctTCATCTGCATGCAATATACTTTTGGTGGTTATTCTATATGTCACTGCATACCCCCCTTTTGTATATAACACTGGAGAAAAAACTTTATCCGGTGAAGGTGGTCGCAAAACCAAGAGGAACGGGGGGGGGGCTAATCGGCAggg                                                                                                                     <  2:867155/147‑1 (MQ=255)
                              cACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAggg                                                                                                                     >  1:283302/1‑150 (MQ=255)
                              cACCTCATCATCTGCATGCAATATAATATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAggg                                                                                                                     >  2:2545814/1‑150 (MQ=255)
                                 cTCATCATCTGCATGCACTATCCGATCGGGGGGTATTCTATATGTCAAGGCACACCCCCCTATAGTATATAACCCTGGAGAATAAAATTTACCCGGGGCATGGGGTCGGAAACCAACGCGGAACGGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                  <  2:301807/150‑1 (MQ=255)
                                 cTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                  >  2:2303276/1‑150 (MQ=255)
                                 cTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                  >  1:302159/1‑150 (MQ=255)
                                 cTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                  >  1:302176/1‑150 (MQ=255)
                                  tcatcaTCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                 >  1:2010047/1‑150 (MQ=255)
                                  tcatcaTCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                 >  1:1479699/1‑150 (MQ=255)
                                  tcatcaTCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                 >  1:2173122/1‑150 (MQ=255)
                                         cTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                                                                          >  2:3581254/1‑150 (MQ=255)
                                          tgcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCCGCCGGGAAGGCCGCCCCg                                                                                                         >  2:1897196/1‑150 (MQ=255)
                                           gcacgcaATATACGCTCGGGGGGTATTCTATCTGACACCGCATACCCCCCTATAGTATAGACCCCGGGAGAATAAAATTTAGCCGGTGCATGTGGTCGGAAACCCAAGAGGAAAGGGGGGGGGGCTAATTGGCAGGGAAGGCCGCCCCgg                                                                                                        <  2:1904882/150‑1 (MQ=255)
                                            gatgctATATACTATCGGGGGTTATTCTATATGTCAACGCAGACCCCCCTATAGTATATCACACTGGAGAATAAAATTCATGCGGTGCATGTGGTCGGAACACACAGCGGCCCGGGGGGGGGGCTAAGCGGCAGGGAAGGCCGCCCCGGa                                                                                                       <  2:3603003/149‑1 (MQ=255)
                                            catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                                                       >  2:1842640/1‑150 (MQ=255)
                                              tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                     >  1:3762620/1‑150 (MQ=255)
                                              tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                     >  1:616903/1‑150 (MQ=255)
                                                caATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                                                                   >  1:3545989/1‑150 (MQ=255)
                                                 aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAATACATAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGATGGCCGCCCCGGATAgcg                                                                                                  >  1:474577/1‑150 (MQ=255)
                                                 aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                  >  2:210567/1‑150 (MQ=255)
                                                 aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                  >  2:1618448/1‑150 (MQ=255)
                                                 aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                  >  1:256018/1‑150 (MQ=255)
                                                 aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                  >  2:366799/1‑150 (MQ=255)
                                                 aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                  >  1:2906519/1‑150 (MQ=255)
                                                   tataCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                                                                >  1:3447630/1‑150 (MQ=255)
                                                     taCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACCCCGCAGCATAAACTCCACCCGGTGAAGGGGGCCGGAAACCCCCCCGCAAAGGGGGGGGGGCTAAGCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                                                              <  2:843518/150‑1 (MQ=255)
                                                            ggggggTCTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTCATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                                                       >  1:1605451/1‑150 (MQ=255)
                                                            ggggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                                                       >  1:2299441/1‑150 (MQ=255)
                                                             gggggTAGTCTATATGTCACCGCACCCCCCCCTATAGTAGACACCACCGGAGCACAAACTTCCACCGGTGACTGCGGTCGCCACACAAAGAGCACAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                                                      <  2:3296973/150‑1 (MQ=255)
                                                             cgggcgaTTCTATATGCCAACGCATACCCCCCTACAGTAGACAACACCGGAGACTAAACTCTAGCCGGTGAAGGCGGACGGAAAACAACCCGGAAAGGCGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                                                      <  2:2234526/144‑1 (MQ=255)
                                                               gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGCACGGGGGGGGGGGCTTATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCCGAAGGa                                                                                     >  2:1677833/1‑150 (MQ=255)
                                                               gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGa                                                                                     >  2:1111799/1‑150 (MQ=255)
                                                                ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAATGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                                   >  2:1047112/1‑150 (MQ=255)
                                                                ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                                   >  2:3486672/1‑150 (MQ=255)
                                                                  tATTCTATACGTCAATGCATACCCCCCTATAGTATATAACCCGGGAGAATAAAATTTCTCCGGTGAATGTGGTCGGAAAACACAGCCGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                                                                 <  2:220456/150‑1 (MQ=255)
                                                                    ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                                                                >  1:2408861/1‑150 (MQ=255)
                                                                    atCTATAGGTCCACCCATACCCCCCTATAGCATACAACACCGGAGCATAAAATTTCCCCGGGGAAGGTGGTCGGAAACCACACAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                                                               <  2:3849383/149‑1 (MQ=255)
                                                                     tCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                                                              >  2:1069981/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAAtt                                                                            >  2:320581/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                                            >  2:1380751/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                                            >  2:320675/1‑150 (MQ=255)
                                                                        atatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAttt                                                                           >  1:1610296/1‑150 (MQ=255)
                                                                        atatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAttt                                                                           >  1:2775069/1‑150 (MQ=255)
                                                                          atGTCAAGGCATACCCCCCTATAGTATTTTTCAGTGGAGAATAAAATTTATGGGGTGCATGTGGTCGGAACACATAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGGGGCAGAAGGAATCAGTATTTCCa                                                                        <  2:925085/150‑1 (MQ=255)
                                                                           tctCACTGCATACCCCCCTATAGTATATACCACCGGAGAAGACAATTCCCCCGGTGACTGTGGGCGGACACCCCCGCGGAACGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                                                        <  2:274918/148‑1 (MQ=255)
                                                                           tGTCAATCCATACCCCCCTATAGTATACAACCCGCCAGAATAACCTATAACCGGTGAATGTGGTCGGAAACCAACCAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                                                        <  2:2548760/150‑1 (MQ=255)
                                                                            gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                                                       >  1:136273/1‑150 (MQ=255)
                                                                            gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                                                       >  1:136051/1‑150 (MQ=255)
                                                                            gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                                                       >  1:2955872/1‑150 (MQ=255)
                                                                            gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                                                       >  1:2194083/1‑150 (MQ=255)
                                                                             tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                      >  2:1678021/1‑150 (MQ=255)
                                                                               aaTGGATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGTAAGGGGGGGGGGCTAATCGGGAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGTAATCAGAATTTGCAGGTc                                                                    >  2:2435495/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAGATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                    >  2:3391101/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCCGAAGGAATCAGAATTTCCAGGTc                                                                    >  1:2925066/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAGGGAATCAGAATTTCCAGGTc                                                                    >  2:1425081/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                    >  2:2722651/1‑150 (MQ=255)
                                                                                aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                                   >  2:1215520/1‑150 (MQ=255)
                                                                                 tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg                                                                  >  1:1788068/1‑150 (MQ=255)
                                                                                   cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc                                                                >  1:3233488/1‑150 (MQ=255)
                                                                                   cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAGAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc                                                                >  1:3233307/1‑150 (MQ=255)
                                                                                       ccccccTATAGTACATAACCCTGGAGAATAAAATGTCGCCGGTGAAAGTGGTCGGAAACCAAAGAGGAAAGGGGGGGGGGATAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGAGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc                                                            <  2:2912102/150‑1 (MQ=255)
                                                                                          cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                         >  1:941164/1‑150 (MQ=255)
                                                                                           ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                                                        >  1:244526/1‑150 (MQ=255)
                                                                                            cTATAGTATATAACACCGGAGAATAACAATTATCCGGTGAATGTGGTCGGAAAACAAAGAGGACAGGGGGGGGGGCTACTCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                                                       <  2:1282222/150‑1 (MQ=255)
                                                                                                 gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGGAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGTAGGAATCAGAATTTGGAGGTCAGAGGGGGTGCTAGTTGc                                                  >  2:1974863/1‑150 (MQ=255)
                                                                                                  catataACCCGGGAGAATAACATACCCCCGGTGAACGTGGTCGGCACACCCCCAGGCCAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAGAGCGGGCGGCGGAAGGAAGCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                                 <  2:2007374/149‑1 (MQ=255)
                                                                                                       aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg                                            >  2:2419866/1‑150 (MQ=255)
                                                                                                       aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg                                            >  1:2914632/1‑150 (MQ=255)
                                                                                                                 aagaacaTTTCTCCGGTGACTGTGGTCGGAAACCCCAGCCGCACGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcat                                  <  2:482402/144‑1 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                >  1:3253743/1‑150 (MQ=255)
                                                                                                                     aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGTGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                              >  2:3099905/1‑150 (MQ=255)
                                                                                                                                 tGAATTGGGTCGGAACAGAGAGATGTAAGTGGGGTGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGTGCTGCAGAAGGAATCATAATTTCAAGTTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATAATCTGTTGGGGTGTCGTa                  <  2:1149640/150‑1 (MQ=255)
                                                                                                                                          tCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACttt         >  1:2608674/1‑150 (MQ=255)
                                                                                                                                          tCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACttt         >  2:554259/1‑150 (MQ=255)
                                                                                                                                          tCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGGTAAAATAATAGGTTGGGGTGTCGTACCACACttt         >  1:3047104/1‑150 (MQ=255)
                                                                                                                                             gAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAcc      >  1:113131/1‑150 (MQ=255)
                                                                                                                                               aaaCAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTg    >  1:3037100/1‑150 (MQ=255)
                                                                                                                                                aaCAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGc   >  2:985591/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCC  >  NC_000913/379856‑380150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: