Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 8.2% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA 8.2% 259.2 / 14.8 113noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (61/39);  new base A (4/5);  total (66/47)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

Only 100 of 118 total aligned reads displayed.

TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287001‑1287298
                                                                                                                                                    |                                                                                                                                                     
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGTTCGTTGCATGAGGCAACGCTCTCTCGCTTGCGCTCGa                                                                                                                                                      >  2:906907/1‑150 (MQ=255)
gtCCCCGCATGGGCAGAACTTTTGCGTGCGGATTCGCTTGAGACTTCAGGGACTGTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                      <  1:2663035/149‑1 (MQ=14)
    ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACCCACACACGCAGGCGCTCGAGTCg                                                                                                                                                  >  1:3290307/1‑150 (MQ=14)
             cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCATGGt                                                                                                                                         >  1:3494066/1‑150 (MQ=255)
              aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAAAGCACTCTCGCGGGCGCCACAGTCGAACCGTGGTc                                                                                                                                        >  1:1064911/1‑150 (MQ=14)
              aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTc                                                                                                                                        >  1:2329973/1‑150 (MQ=255)
              aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACACTCCCTGGCGCGCGAGTCGAACCATGGTc                                                                                                                                        >  1:1129933/1‑150 (MQ=14)
                aaTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAATCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCAAGCTGGCGCTCGAGTCGAACCTTGGTCGa                                                                                                                                      >  1:1261872/1‑150 (MQ=255)
                    tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCACTCACGCTGGCGCTCGAGTCGAAACTTGGTCGaatca                                                                                                                                  >  1:596367/1‑147 (MQ=14)
                         ttGCGGATTCGTTTGAGATTTCAGGGTCTTTTGAAAGTGATGGTGGTGGTGGTAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCa                                                                                                                             <  2:3842473/150‑1 (MQ=11)
                         gggCGGAGTCGATTGAGAGTTCCGGGCCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCGTCGCCCTTCTGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTGGGTCGAAGCTTCTCa                                                                                                                             <  2:3842469/148‑1 (MQ=11)
                             ggATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCTCACTCGCTGGCGCTCGAGTCGAAACTTGGTCGGAGCTTCTAAACCt                                                                                                                         >  2:3718520/1‑150 (MQ=14)
                                 tCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGATATCTCGCTGGCGCTAGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTccc                                                                                                                     >  1:2197364/1‑150 (MQ=14)
                                 tCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCGCACTCCCAGGCGCACGAGTCGAACCATGGTCGAAGCTTATCAtacaaaac                                                                                                                     >  1:374565/1‑143 (MQ=14)
                                    cTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGc                                                                                                                  >  1:2249446/1‑150 (MQ=255)
                                      tGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCCGCAACGCTCGCACGCGGGCGCGCGAGTCGAACCATGGTCGAAGCATCACAAACATCCCCGCac                                                                                                                >  1:1976808/1‑149 (MQ=14)
                                       gagaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCAACCATCCCCGCATg                                                                                                               >  2:543303/1‑150 (MQ=255)
                                       gagaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCagg                                                                                                               >  2:543321/1‑148 (MQ=255)
                                          ggTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACCCTGGCGCGCGATTCGAACCGTGGTAGAAGCTTCTCAACCTTCCCCGccggggg                                                                                                            >  1:553093/2‑144 (MQ=14)
                                           gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGATCTCTCGCTGTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCAACCTTCCCCGctgagggc                                                                                                           >  2:465834/1‑143 (MQ=11)
                                           gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCACACTAGCTGGCCCGCGAGTCGAACCATGGTCGAAGCATATCAAACGTCCCCGCAAGGGcc                                                                                                           >  1:291140/1‑149 (MQ=14)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCGCGAGTCGAACCATGGTCCAAGCTTCACAAACTTACCCCCAATGGCAGAAAATTTaa                                                                                                 >  2:2818223/1‑150 (MQ=11)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGACGAAGCATCTCATCAATAACCCCATGGGCATAATATTTaa                                                                                                 >  2:2818211/1‑150 (MQ=11)
                                                         gAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                             <  1:36263/150‑1 (MQ=39)
                                                          aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGGGGCAACGCTCTCTCGGTGGCGCTCGATTGGTACCTTGGTCGTAGGTTGTGATAGTTGCCCGGATGGGGAGAATatttattttgg                                                                                            >  2:872876/1‑147 (MQ=11)
                                                          aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCGGGCGCTCGAGTCGAAACTTGGTCGCAGCTTATCAAACTTCACCGCAGGGGGCGGATATTTAATTGCg                                                                                            >  1:2872883/1‑150 (MQ=14)
                                                           aaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTAGGTCGAAGCTTAGCATCCATCCCCGCCTGGGCACAATATTTAATTTCgg                                                                                           >  2:1027550/1‑150 (MQ=11)
                                                           aaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCACACGCTTGCGCTCGAGTCGAACCATTGTAGAAGCTTCACAAACATACCCGCATGGGCAGACTATTTAATTGCgg                                                                                           >  2:1027536/1‑150 (MQ=11)
                                                            aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCTCTCACGCTCGCGCTCGACACGAAACTTGGTCGAAGCTTATCAACCTTACCCCCAAGGGCAGAAAATTTAATTGAggg                                                                                          >  2:1137754/1‑149 (MQ=11)
                                                            aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAAAGCTCACTCGCTGGCGCTCGAGTCGAAACTTGGTAGAAGCTTATAATACTTACCCGCAAGGGCAGAATATTTAAATGCGGa                                                                                          >  2:1137973/1‑150 (MQ=14)
                                                             gTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAt                                                                                         <  1:1529540/150‑1 (MQ=37)
                                                             gTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAt                                                                                         <  1:3725610/150‑1 (MQ=38)
                                                                      gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                                <  2:2322833/150‑1 (MQ=255)
                                                                            gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCGTTGTCGGAGCTTCTCATACATGCCCGCAGGGGCCGGAAATTTAATTGCGGCTTCGGTGGGGAGttga                                                                          >  2:1052689/1‑148 (MQ=11)
                                                                             aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                         <  1:3889981/150‑1 (MQ=255)
                                                                              aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                                        <  1:2688809/150‑1 (MQ=255)
                                                                              aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                                        <  1:1987589/150‑1 (MQ=255)
                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                      <  1:1395958/150‑1 (MQ=255)
                                                                                 aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGGATATTTGATTGCGGTGTCGTTGGGTAGTTCAggggg                                                                     >  2:2191820/1‑148 (MQ=14)
                                                                                 aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGGATATGGGAGTGCGGATTCGTTGGGGAGTTCAggggg                                                                     >  2:3295784/1‑148 (MQ=11)
                                                                                 aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTAATCCTTCCCCGCATGGGAAGGATAGTTGAGTTGGGCTTAGTTGGGTAGTTGAggggc                                                                     >  2:1020464/1‑148 (MQ=11)
                                                                                  ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGGCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGGGGGGAGTTCAGGGACt                                                                    >  2:2584245/1‑150 (MQ=11)
                                                                                    tcTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                                  <  1:2611992/149‑1 (MQ=255)
                                                                                    aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                                  <  1:3495881/150‑1 (MQ=255)
                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTGGGTAGTTCAGGGACTTTTgta                                                              >  2:2611933/1‑148 (MQ=11)
                                                                                         gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                             <  1:2214030/150‑1 (MQ=255)
                                                                                         gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                             <  1:1542921/150‑1 (MQ=255)
                                                                                         gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                             <  1:1180619/150‑1 (MQ=255)
                                                                                          cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTAATTGCGGATTGGTGGGGAAGTTCAGGGGCTTTTGagag                                                            >  2:211358/1‑150 (MQ=14)
                                                                                             tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                      <  1:77818/57‑1 (MQ=33)
                                                                                             tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                      >  2:77818/1‑57 (MQ=33)
                                                                                             tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         <  1:3036341/150‑1 (MQ=255)
                                                                                             tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         >  2:1952018/1‑150 (MQ=255)
                                                                                                  gcTTCGCCCTTCGGGTCGTTTCCAGCGGCAACGCTCTATAGCTTGCGCTCGAGTCGAAACGTTGTCGAAGCTTCTCAAACTTACCCGCAGCGGCAGAAAATTAAAATTCGGATTAGTTGGGAAGATGAGGGGAGTTTGAAAGTGAtggtg                                                    >  2:3643518/1‑150 (MQ=9)
                                                                                                  gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAc                                                                                                                                               <  1:2677353/59‑1 (MQ=35)
                                                                                                  gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAc                                                                                                                                               >  2:2677353/1‑59 (MQ=35)
                                                                                                  gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCGCATGGGCAGAAAATTTAATTTCGGAATCGTGTGGAAGTTAAGGGACTTTTTAAAGTGAtggtg                                                    >  2:1583886/1‑150 (MQ=14)
                                                                                                  gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCTCACTCGCTGGCGCACGAGTCGAACCGTGGTCGTAGCGTCTCATCCGTCCCCGCATGGGGACAAAATTTAAAATAGCAATCGGTGTGAACATCAGGGGCTGTTGAAAGTgtggggg                                                    >  2:3643522/1‑144 (MQ=2)
                                                                                                    ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCCCAGGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACGTTTGACAGTGAtggtggg                                                  >  2:3479256/1‑149 (MQ=255)
                                                                                                        cccTTCGGGTCGGTGCCTGCGGCAACGGTCTCTCGAGTGGGTGAGTGTCGTTCAGTGGTGTTATCTTCCGATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGATGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                              <  1:3659692/150‑1 (MQ=9)
                                                                                                           ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           >  2:2069672/1‑150 (MQ=255)
                                                                                                           ttCGGGTCGTGGCCTGCGCCCTCGCTCTCCCGCCGTCGCCCGAGTCTAACCCTCGTCGAAGCTCCTCCGACGTCCCCGCTTGGGCAGAATATTTCATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  2:337107/150‑1 (MQ=14)
                                                                                                           gtCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGCACCTGGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTAAGTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  1:442903/149‑1 (MQ=17)
                                                                                                           gagagagcGTTGCCGCAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           >  2:286066/8‑150 (MQ=14)
                                                                                                             gagagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAgg                                         >  2:906704/6‑150 (MQ=255)
                                                                                                               gagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTAATTGCGGATTCGTTGGGCAGTTCAGGGACTTTTGAAAGTGAGGGTGGTGGGGGACGGAt                                       >  2:3892192/4‑150 (MQ=14)
                                                                                                                gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                      <  1:2069672/150‑1 (MQ=255)
                                                                                                                gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                      <  2:2795106/150‑1 (MQ=255)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGCACCGTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCCGAAGATTTAATTGCGGATTCGTTGGGGCGTTCAGGGGCTTTTGAGAGTGATGGTGGTGGGGGGAGGATTAGaa                                  >  1:3247391/1‑150 (MQ=14)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                            >  1:2317739/1‑60 (MQ=35)
                                                                                                                     ggCCTGGGGCATCTCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                 <  1:3782599/149‑1 (MQ=255)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                                       <  2:24848/94‑1 (MQ=37)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCt                               >  2:2586626/1‑150 (MQ=255)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTCATTGCGGATTCGGTGGGAAGTTCAGGGACTGTTGAAAGTGATGGTGGTGGGGGAAGGATTAGAAcca                               >  2:1132228/1‑149 (MQ=14)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                                       >  1:24848/1‑94 (MQ=18)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                                       <  2:752875/94‑1 (MQ=18)
                                                                                                                        cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTACTTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                              <  1:1152354/150‑1 (MQ=255)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAATCTTCTCATCCATCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACATTTTAAAGTGTTTGTGGTGGGGGAAGGATTGTAACCTTc                             >  1:3425256/1‑150 (MQ=17)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             >  2:2859/1‑150 (MQ=255)
                                                                                                                              cAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                    <  2:325255/90‑1 (MQ=255)
                                                                                                                              cAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                   <  1:187487/91‑1 (MQ=21)
                                                                                                                                 cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTGGGGCAGAAGATTTAATTTCGCATGCGATGTGCAGGTGAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGATCCGTAGAAGTCGa                     >  2:3602531/1‑150 (MQ=17)
                                                                                                                                 cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                     >  2:3602505/1‑150 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtct                                                                                                                               <  1:2676189/41‑1 (MQ=38)
                                                                                                                                    tctctcGCTGCCGCTCGAGACGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTCATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                  <  2:3191674/150‑1 (MQ=255)
                                                                                                                                      tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtctc                                                                                                                              <  1:2979749/40‑1 (MQ=40)
                                                                                                                                           cTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGaa                                                                               <  1:2056974/82‑1 (MQ=255)
                                                                                                                                           cTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGaa                                                                               >  2:3069665/1‑82 (MQ=255)
                                                                                                                                           cTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGaa                                                                               >  2:2056974/1‑82 (MQ=255)
                                                                                                                                           cTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGaa                                                                               <  1:3069665/82‑1 (MQ=255)
                                                                                                                                           cTGCCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCATAATATTTAATTGCGGATTCGTTTGTTAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGa           <  1:817855/150‑1 (MQ=17)
                                                                                                                                            tgtcgcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt          <  1:1052689/147‑1 (MQ=255)
                                                                                                                                            tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt          <  1:1053121/150‑1 (MQ=255)
                                                                                                                                            tGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt          <  1:1053130/150‑1 (MQ=255)
                                                                                                                                               cgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTa       >  1:3208603/1‑150 (MQ=255)
                                                                                                                                                   cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGt   <  1:1132228/150‑1 (MQ=255)
                                                                                                                                                    gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  >  2:1996852/1‑150 (MQ=255)
                                                                                                                                                    gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  >  2:1545212/1‑150 (MQ=255)
                                                                                                                                                    gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTAGATGACGGCAGATTTAAAGta  >  1:581024/1‑149 (MQ=255)
                                                                                                                                                    gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCATCGAAGTCGATGAAGGCAGATTTAAAGTc  >  1:3508380/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287001‑1287298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: