Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,639 C→T 9.6% pseudogene (1248/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,6390CT 9.6% 308.9 / 14.6 104pseudogene (1248/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base C (53/41);  new base T (6/4);  total (59/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAC  >  NC_000913/1466493‑1466785
                                                                                                                                                  |                                                                                                                                                  
ggCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGt                                                                                                                                                 <  2:23624/150‑1 (MQ=255)
  cGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtga                                                                                                                                               <  2:2649508/150‑1 (MQ=255)
  cGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtga                                                                                                                                               <  2:2649545/150‑1 (MQ=255)
  cGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtga                                                                                                                                               >  2:1860063/1‑150 (MQ=255)
  cGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGGTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtga                                                                                                                                               >  2:3815621/1‑150 (MQ=255)
   gACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtgat                                                                                                                                              >  2:2441152/1‑150 (MQ=255)
     cAGCGCTACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGGTTGACGGCGACAAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtgatga                                                                                                                                            <  2:3283702/150‑1 (MQ=255)
        cgcAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGc                                                                                                                                         <  2:3222706/150‑1 (MQ=255)
           atatgTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGCTTGACGGTGACAAGGCGTTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCAc                                                                                                                                      <  1:1580763/146‑1 (MQ=14)
             cGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGTTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACgg                                                                                                                                    <  1:918553/150‑1 (MQ=255)
             cGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACTCAGATCAACGGTAACGACGCCAccg                                                                                                                                    <  2:603195/150‑3 (MQ=255)
             cGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACgg                                                                                                                                    <  1:918583/150‑1 (MQ=255)
                  gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaac                                                                                                                               <  2:1058200/150‑1 (MQ=255)
                      aCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaaca                                                                                                                           >  1:2949237/1‑150 (MQ=255)
                      aCAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaaca                                                                                                                           >  1:2592248/1‑150 (MQ=255)
                       cAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaacaa                                                                                                                          >  2:1830560/1‑150 (MQ=255)
                        aaGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTAAACAACGAAGGCGAGAGCGCCATCACCAAAGGAGGCACCGGCACGCAGATAAACGGTAACGACGCCACCGCGaataacagt                                                                                                                         >  2:1892713/1‑148 (MQ=11)
                             cACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCCCCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCaa                                                                                                                    >  1:239541/1‑150 (MQ=255)
                             cACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCaa                                                                                                                    >  1:1411998/1‑150 (MQ=255)
                             cACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCaa                                                                                                                    >  1:239724/1‑150 (MQ=255)
                                    aCCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAaccacc                                                                                                             <  2:2674027/150‑1 (MQ=255)
                                        tCACCGATCCGGAGTACATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTg                                                                                                         >  1:3805871/1‑150 (MQ=255)
                                         cACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGa                                                                                                        <  2:1470405/150‑1 (MQ=255)
                                          aCCGATCCGGAGTCCATCGGTATCCAGGTTGACGGTGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCACAACCACCGTTGAc                                                                                                       >  1:2872218/1‑150 (MQ=255)
                                          aCCGATCCGGAGTCCATCGGTATCCAGGTTGACGGTGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGATGAc                                                                                                       >  1:2872312/1‑150 (MQ=255)
                                          aCCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGACGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGAc                                                                                                       >  1:1611924/1‑150 (MQ=255)
                                            cGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg                                                                                                     <  1:2391961/150‑1 (MQ=255)
                                            cGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg                                                                                                     >  2:3547724/1‑150 (MQ=255)
                                              aTCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCa                                                                                                   >  2:1698520/1‑150 (MQ=255)
                                              aTCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCa                                                                                                   >  2:306360/1‑150 (MQ=255)
                                              aTCCGGAGTCCATCGGTATACAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCa                                                                                                   >  2:3658958/1‑150 (MQ=255)
                                                 cGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAgg                                                                                                >  1:1232284/1‑150 (MQ=255)
                                                        cATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc                                                                                         >  1:413166/1‑150 (MQ=255)
                                                        cATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc                                                                                         <  2:2807889/150‑1 (MQ=255)
                                                                   aGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAAAGGAGGCACCGGCACTCAGATAAACGGTAAAGACGCCACCGCGAAAAACAGAGGAAAAACCACAGTTGAAGGCAAAGATTCCACCGGTACgtcca                                                                              >  1:3152933/1‑146 (MQ=255)
                                                                    ggTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGACAt                                                                             >  1:2064223/1‑150 (MQ=255)
                                                                    ggTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAt                                                                             <  1:597793/150‑1 (MQ=255)
                                                                      ttGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACTCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTg                                                                           >  1:2080990/1‑150 (MQ=255)
                                                                      ttGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTg                                                                           >  1:1404543/1‑150 (MQ=255)
                                                                           ggCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGc                                                                      >  1:3791477/1‑150 (MQ=255)
                                                                              gACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCACCGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAAt                                                                   <  1:489247/150‑1 (MQ=255)
                                                                                      ggTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGaa                                                           <  1:2441152/150‑1 (MQ=255)
                                                                                         tgttAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGt                                                        <  1:3547724/150‑1 (MQ=255)
                                                                                             aataacGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCt                                                                         <  1:618133/126‑1 (MQ=18)
                                                                                             aataacGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCt                                                                         >  2:618133/4‑129 (MQ=18)
                                                                                             aacaacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGAtt                                                    >  1:1360242/1‑150 (MQ=255)
                                                                                             aacaacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGAtt                                                    >  1:3328382/1‑150 (MQ=255)
                                                                                               taacGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCa                                                  >  1:2908474/2‑150 (MQ=255)
                                                                                               taacGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCa                                                  >  1:2584433/2‑150 (MQ=255)
                                                                                               taacGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCa                                                  >  1:2584574/2‑150 (MQ=255)
                                                                                               caacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTCACGGTGATTACGCCACTGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCa                                                  <  1:1783835/150‑1 (MQ=255)
                                                                                               caacGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCa                                                  <  1:1557949/150‑1 (MQ=255)
                                                                                                 acGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaacaacaac                                                                                                                         <  1:2660916/77‑1 (MQ=255)
                                                                                                   gAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGAc                                              >  1:493842/1‑150 (MQ=255)
                                                                                                   gAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGAc                                              >  1:698702/1‑150 (MQ=255)
                                                                                                   gAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGAc                                              >  1:1758623/1‑150 (MQ=255)
                                                                                                    aaGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACg                                             >  1:704449/1‑150 (MQ=255)
                                                                                                       gCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATAGCTGGCAATAACGGGAAGGTGATTCAGGACGGCg                                          >  1:3557663/1‑150 (MQ=255)
                                                                                                       gCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATGAACGGTGAGGACGCCACGGCAAACAACACCGGCAAAACCACCGTTGACGGCAAGGAGGCCCCCGGGCCGGACCGTGCGGGCACTCACGGGACGGGGCGGCAGGACGGCg                                          >  2:2240698/1‑150 (MQ=14)
                                                                                                       gCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCg                                          <  2:2584574/150‑1 (MQ=255)
                                                                                                       gCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCg                                          <  2:2908474/150‑1 (MQ=255)
                                                                                                       gCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCg                                          <  2:2584433/150‑1 (MQ=255)
                                                                                                        cGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGa                                         <  1:2065611/150‑1 (MQ=255)
                                                                                                         gagaGCGCCATCACCAACGGTGGCACCGGCACGCAGATAAACGGTGAAGACGCCACGGCAAAAAACACCGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCTGGACGGCgca                                        >  1:345096/1‑148 (MQ=17)
                                                                                                           gagCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCt                                      <  2:2949237/150‑1 (MQ=255)
                                                                                                           gagCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCt                                      <  2:2592248/150‑1 (MQ=255)
                                                                                                            agCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAACACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTg                                     <  2:239541/150‑1 (MQ=255)
                                                                                                            agCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTg                                     <  2:1411998/150‑1 (MQ=255)
                                                                                                            agCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTg                                     <  2:239724/150‑1 (MQ=255)
                                                                                                             gcgcCATCACCAACGGTGGCACCGACACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTgg                                    >  2:1095238/1‑150 (MQ=255)
                                                                                                              cgcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGa                                   <  2:2886335/150‑1 (MQ=255)
                                                                                                              cgcCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGa                                   <  2:2028887/150‑1 (MQ=255)
                                                                                                               gcCATCACCTACGGTGGCACCGGCACGCAGATTAACGTTGATGACGCCACGGCAAACCACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGAt                                  <  2:2483095/150‑1 (MQ=255)
                                                                                                                 cATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGt                                >  1:3511212/1‑150 (MQ=255)
                                                                                                                 cATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGt                                >  1:826398/1‑150 (MQ=255)
                                                                                                                 cATCACCAACGGTGGCACCGGCACGCAGATAAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGt                                >  1:37476/1‑150 (MQ=255)
                                                                                                                  aTCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGACGGTGAGTCAGGACGGCGATCTGGAGGTc                               >  2:2744460/1‑150 (MQ=255)
                                                                                                                  aTCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTc                               >  2:3002584/1‑150 (MQ=255)
                                                                                                                  aTCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTc                               >  1:2355601/1‑150 (MQ=255)
                                                                                                                    cACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAg                             <  1:3003864/150‑1 (MQ=255)
                                                                                                                     aCCAACGGTGGCACCGGCACGCAGATTTACGGTGATGACGCCACGGCAACCAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgc                            <  1:1020465/150‑1 (MQ=255)
                                                                                                                     aCCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATGGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgc                            >  2:3804150/1‑150 (MQ=255)
                                                                                                                     aCCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATGGAAGGAAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgc                            >  2:3003864/1‑150 (MQ=11)
                                                                                                                      ccAACGGTGGCACCGGCACTCAGATAAACGGTGAAGACGCCACCGCAAAAAACAAAGGAAAAACCACCGTTGAGGGCAAGGATTCCACCGGTACGGAAATAGCTGGCAATAACGGGAATGTGATTCTGGACGGCGAGCTGGATGTAAgcg                           >  1:2931087/1‑150 (MQ=14)
                                                                                                                      ccAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcg                           >  1:3850214/1‑150 (MQ=255)
                                                                                                                      ccAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcg                           >  2:3244467/1‑150 (MQ=255)
                                                                                                                       cAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAaac                                                                                                                               <  2:2678449/49‑1 (MQ=255)
                                                                                                                       cAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATaa                                                                 <  1:1540201/111‑1 (MQ=255)
                                                                                                                       cAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcgg                          >  2:117829/1‑150 (MQ=255)
                                                                                                                       cAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcgg                          >  1:1958738/1‑150 (MQ=255)
                                                                                                                        aaCGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGTACGGCGATCTGGATTTCAgcgtc                         >  2:392953/1‑148 (MQ=255)
                                                                                                                            gTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAgcggcggcg                     >  1:1917318/1‑150 (MQ=255)
                                                                                                                             tGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGcggcggcgg                    >  2:1817772/1‑150 (MQ=255)
                                                                                                                             tGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGcggcggcgg                    >  1:3865139/1‑150 (MQ=255)
                                                                                                                                 aCCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCAc                >  1:1447217/1‑150 (MQ=255)
                                                                                                                                       aCGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAtt          <  1:3815621/150‑1 (MQ=255)
                                                                                                                                       aCGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAtt          <  1:1860063/150‑1 (MQ=255)
                                                                                                                                        cGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTACACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTg         <  2:3805871/150‑1 (MQ=255)
                                                                                                                                         gCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAATACCTCCGTTGACGGCAAGGATTCCTCCGGTTCGGAAATTGCTTGCAATAACGGGAATGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa        <  2:3054399/150‑1 (MQ=17)
                                                                                                                                               tatcGGCGATGTCGCCGCGGAATACTACATCGGGAACTCCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTTATGGCAATAACGGTAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAc  <  2:3622146/147‑1 (MQ=14)
                                                                                                                                                  |                                                                                                                                                  
GGCGACAGCGCAACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAC  >  NC_000913/1466493‑1466785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: