Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 12.5% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT12.5% 501.7 / 10.0 209intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (77/95);  new base T (21/14);  total (98/111)
Fisher's exact test for biased strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAC  >  NC_000913/2177096‑2177390
                                                                                                                                                     |                                                                                                                                                 
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATg                                                                                                                                                   >  2:3840595/1‑150 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCAACTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAat                                                                                                                                               >  1:830643/1‑150 (MQ=255)
      ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                             >  1:642191/1‑150 (MQ=255)
           ccTTGCATCGGTTCGAGATTGTGAATAGTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                        <  2:351011/149‑1 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                   <  2:2117824/150‑1 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                   <  2:900895/150‑1 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                   <  2:68515/150‑1 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                   <  2:3918445/150‑1 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                   <  1:3731852/150‑1 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATTAAATTATTTGTTTTTa                                                                                                                                   >  1:3586325/1‑150 (MQ=255)
                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                                  <  1:3305212/150‑1 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGGTGTTCAGCATCTGCTTTGTCGCTACCACGTACATTTTCATATCCTGTCGTTTGTGTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAata                                                                                                                               <  1:1516137/150‑1 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                             <  1:2123568/150‑1 (MQ=255)
                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                            <  2:970661/150‑1 (MQ=255)
                         tagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATAATTTTTTTTTAATATAgat                                                                                                                          >  1:2705259/2‑149 (MQ=255)
                           gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                        <  2:242921/150‑1 (MQ=255)
                           gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                        <  2:2005729/150‑1 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                  <  2:1527869/150‑1 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAgataacgataac                                                                                                               >  2:117228/1‑150 (MQ=255)
                                        gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACaaaa                                                                                                           >  2:1251467/1‑150 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                          <  2:3622028/150‑1 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATaaaaaaaa                                                                                                          >  1:811478/1‑150 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGGGTTAGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATGTGGATAACGAAACCaaaaa                                                                                                          >  1:2797238/1‑150 (MQ=255)
                                          aTGCCGGAACCTCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                         <  1:1248577/150‑1 (MQ=255)
                                            gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                       <  2:2695097/150‑1 (MQ=255)
                                            gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                       <  2:3359093/150‑1 (MQ=255)
                                            gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                       <  2:3451896/150‑1 (MQ=255)
                                             ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAATTATTTTTTTTTAAATATGGAGGTAAAGATaaaaaaaaacac                                                                                                      >  1:3023840/1‑146 (MQ=255)
                                             ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTCGAGTTCCAAAAATAATGAAAGTATTTGTTTTTAAATATCGCGATAACGATCACAAAAACGAc                                                                                                      >  2:2787061/1‑150 (MQ=255)
                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAATAATTAAAATATTTTTTTTTAAATATAGAGATAACGATCACAAAAACcaca                                                                                                     >  1:885218/1‑150 (MQ=255)
                                                gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAAAAAAGAAAATATTTTTTTTTTAAAATAGAGATAAAGATAAAAAAAAAGACaag                                                                                                   >  1:3335250/1‑149 (MQ=255)
                                                 aaCCGCATAACCCCCGCGCTGTGCGTTTTTCAGCATCTGCTTTGACGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATCAAATTATTTGTTTTTAAATATCTAGATAACGATCACAAAAACGACAata                                                                                                  <  2:1058113/150‑1 (MQ=255)
                                                    cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAAATATTTTTTTTTAAATATAGATATAAAGATCACAAAAAAGAAAATATGa                                                                                               >  2:3864163/1‑150 (MQ=255)
                                                      aaTAACCCCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATAACCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                             <  2:3665682/149‑1 (MQ=255)
                                                        tAACCGCCGCGCTGTGCGTTGTTCAGCATCTACTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAat                                                                                           >  2:1966099/1‑150 (MQ=255)
                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                                      <  2:282670/150‑1 (MQ=255)
                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAATATAAATAAAATATTTTTTTTTAAATATGGAGATAAAGATAAAAAAAAAGACAATATTAAAaatatta                                                                                      >  1:2201095/1‑149 (MQ=255)
                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAATTTAATTATTTTTTTTTTAATATAGAGATAAAGATCACAAAAAACAAAAAAATAAAattatta                                                                                      >  1:1877045/1‑149 (MQ=255)
                                                             gccgcGCTGTGCGTTGTTCAGAATCTGCTTTGTCGATACCACGTAAATTTTCATATCCTGGCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                                      <  2:1761493/150‑1 (MQ=255)
                                                               ggcgcTGTGGGTTTTTCTGTTTCTGTTTTTTCGATACCACGTAATTTTTCATACCGTGTGGTTTTTTTTCGATTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACTATCACAAACACGACAATATGAAAATTATTCga                                                                                    <  1:3393448/149‑1 (MQ=255)
                                                                gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                                   <  2:2072911/150‑1 (MQ=255)
                                                                   cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATATTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATATGTTTTTAAATATAGAGATAACGATCACAAAAACGACAAAATGAAAATTAATCgaggag                                                                                >  2:2246753/1‑150 (MQ=255)
                                                                    tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAACGACAATATGAAAATTATTAGAGGAGt                                                                               >  2:1432127/1‑150 (MQ=255)
                                                                    tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGt                                                                               >  2:3872470/1‑150 (MQ=255)
                                                                    tgtgCGTTGTTCAGCATCTGCTTTGTCGATACAACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAAAATAAATAAAATATTTGTTTTTAAATAACGAGAAAAACATCACAAAAACAACAAAATGAAAAATATTAGATGAGt                                                                               >  2:3367874/1‑150 (MQ=255)
                                                                      tgCGTTGTTCAGAATCGGCTTTGTCGACCCCACTCAATTTTTCATATCCTGCCGTTTTTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCTAGATACCGATCAAAAACACGACAATATGAAAATTATTCGAGGAGTGa                                                                             <  1:292106/150‑1 (MQ=255)
                                                                        cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                           <  2:3119296/150‑1 (MQ=255)
                                                                         ggtgttCCGCATCGGCTTTGTCGATCCCCCGTACATTTTCATATCCGGTCGTTTGTTTTCGATTTCAAAATATACTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                          <  2:776139/148‑1 (MQ=255)
                                                                             ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTTAATATAGAGATAAAAAAAAAAAAAAACACAAAAATTAAAATATTGGAGGGGGGAAATGCaa                                                                      >  1:1971937/1‑150 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                <  2:1485630/150‑1 (MQ=255)
                                                                                   aTCTGATTTGTCGATACCACTTACTTTTTCATATCCTGTCGTTTGTTTTCTATTACAAAATTTAATGAATTTTTTTTTTTTTAAATATCGTGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                <  1:460304/150‑1 (MQ=255)
                                                                                    tCTGCTTTTTCGATACCACGTAAATTTTCATATCCTTTCTTTTGTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAATTATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                               <  2:2705913/150‑1 (MQ=255)
                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                               >  2:2646900/1‑150 (MQ=255)
                                                                                         tttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTCAAAATATAATTAAAATATTTGTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAAAATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGccac                                                          >  2:2441406/1‑148 (MQ=255)
                                                                                               gATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                    <  2:2258478/150‑1 (MQ=255)
                                                                                                 tACCACTTACATTTTAATTTCCTTTCTTTTTTTTTCTATTTAAAAATATATTTAAATTTTTTTTTTTTAATTATCTATATAACTAAAAAAAAAACGAAAATATTAAAATTATTCGTGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                  <  1:1303844/150‑1 (MQ=255)
                                                                                                 tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGAGGAGTGAAAGGCAAAAAAACGGCCTACCCCTAgg                                                  >  2:2820918/1‑150 (MQ=255)
                                                                                                    cccGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                               <  2:305559/148‑1 (MQ=255)
                                                                                                    cACGTACATTTTCATCTCCGGTCGTTGGTTTTCGATTTCAAATTTTAATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAACAACGGCCTCCCGATAGGGaa                                               <  2:2472567/150‑1 (MQ=255)
                                                                                                      attACTTTTACTTATCCTGTGTTTTTTTTTTTTTTAAAAAATATATTTAATTTATTTTTTTTTTATTATCGATATAACTATCAAAAAAACGACATTATGAAAATTATTCGAGGAGTTAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                             <  1:3649477/148‑1 (MQ=255)
                                                                                                          cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                         <  2:3542347/150‑1 (MQ=255)
                                                                                                          cATTTTCATAGCCTGCCGTTTGTTTTCGATTTCAAAATATACTGAAATTATTTGTTTTTAAATATCGAGATACCGATCCCAAAACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                         <  2:131730/150‑1 (MQ=255)
                                                                                                          cATTTACATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                         <  2:1706345/150‑1 (MQ=255)
                                                                                                          atttttCATATCCTGTGTTTTTTTTTCTATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCTATATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                         <  1:726388/148‑1 (MQ=255)
                                                                                                           aTTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGttt                                        <  2:1800770/150‑3 (MQ=255)
                                                                                                             tttCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGAGGGGAGCCGTAGCa                                      >  2:2179108/1‑150 (MQ=255)
                                                                                                               tCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                    <  2:222949/150‑1 (MQ=255)
                                                                                                                   atCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTAAATTATTTTTTTTTAAATAACGAGATAACGATCACAAAAAAGAAAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAACGCCACCCGATAGGGAAGCCCTAGCAAAGTgc                                >  2:848135/1‑150 (MQ=255)
                                                                                                                   atCCTGTCGTGTGTTTTCGATTTCAAAATAAAATGAAATTATTTTTTTTTAAAAATAGAGATAACCATAACAAAAACGAAAAAATGTAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGCCACCCCATAGGGAACCCCTAGCAAAGTgc                                >  2:526731/1‑150 (MQ=255)
                                                                                                                    tCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                               <  2:2334568/150‑1 (MQ=255)
                                                                                                                      cgggcGTTTGTTTTCGATTTCAACAGATCATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                             <  2:796632/146‑1 (MQ=255)
                                                                                                                      cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                             <  2:2577298/150‑1 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                        <  2:1877346/150‑1 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                        <  2:2201095/150‑1 (MQ=255)
                                                                                                                           gtttgtttTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                        <  2:1877045/150‑1 (MQ=255)
                                                                                                                            tttgtttTCTATTTCAAAATATAATGAACTTATTTGTTTTTAAATAGCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAACACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                       <  1:2338660/150‑1 (MQ=255)
                                                                                                                               gtttTCTATTACAAAATATAATGAAATTATTTTTTTTTAAATATCGATAAAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                    <  1:2699732/150‑1 (MQ=255)
                                                                                                                                ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                   >  2:3143340/1‑150 (MQ=255)
                                                                                                                                 tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                  >  2:1273930/1‑150 (MQ=255)
                                                                                                                                   tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                >  1:879954/1‑150 (MQ=255)
                                                                                                                                   tCGATTAAAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                <  1:2010746/150‑1 (MQ=255)
                                                                                                                                     gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa              <  1:1055758/150‑1 (MQ=255)
                                                                                                                                      aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATTCCGGAt             >  1:3312238/1‑150 (MQ=255)
                                                                                                                                        ttCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc           <  2:3335273/150‑1 (MQ=255)
                                                                                                                                        ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc           <  2:3335169/150‑1 (MQ=255)
                                                                                                                                        tacAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc           <  2:811641/148‑1 (MQ=255)
                                                                                                                                         tCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATgtg          <  1:2787061/150‑3 (MQ=255)
                                                                                                                                         tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg          <  2:3023840/150‑1 (MQ=255)
                                                                                                                                         tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg          >  2:73326/1‑150 (MQ=255)
                                                                                                                                          cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg         >  1:2390094/1‑150 (MQ=255)
                                                                                                                                          cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg         >  2:3031995/1‑150 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGCGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt        >  1:2149455/1‑150 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt        <  1:1091825/150‑1 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt        >  2:3212586/1‑150 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt        <  1:3192576/150‑1 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTGTTAAATATCGCGAGAACGATCACAAAAACGACAATATGAAAATTCTTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt        >  1:184268/1‑150 (MQ=255)
                                                                                                                                            aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg       <  1:2590384/150‑1 (MQ=255)
                                                                                                                                             aaTATAATGAAATTATTTGTTTTTAACTATCGAGATAACGATCACAAACACGACAATCTGAAAATTATTCGAGGAGTGAAAGGCAAAAACACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt      <  2:3506697/150‑1 (MQ=255)
                                                                                                                                                 taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc  >  2:404594/1‑150 (MQ=255)
                                                                                                                                                 taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc  <  1:947354/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAC  >  NC_000913/2177096‑2177390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: