Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,762 T→C 7.3% intergenic (+369/+346) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,7620TC 7.3% 295.8 / 8.9 110intergenic (+369/+346)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (59/40);  new base C (3/5);  total (64/46)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

CCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACG  >  NC_000913/2304639‑2304910
                                                                                                                           |                                                                                                                                                    
ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCATGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                            <  1:1849185/150‑1 (MQ=14)
ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                            <  1:1059683/150‑1 (MQ=31)
ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                            >  1:3919844/1‑150 (MQ=14)
 cccGTAGGTCCGATAAGGCGGTCACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGAc                                                                                                                           <  1:2768063/150‑1 (MQ=12)
     gaGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATcc                                                                                                                       <  2:2708391/149‑1 (MQ=17)
      aGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCa                                                                                                                      <  1:1476862/150‑1 (MQ=18)
        gTCGGATAAGGCGGTTACGCCGCCTCCGACATCTCACGCCCACGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                                                                                                    <  2:3806844/150‑1 (MQ=255)
            gagaaGGCGGTTACGCCGCATCCGACATCGAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTcc                                                                                                                <  2:1675210/147‑1 (MQ=255)
            gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTcc                                                                                                                <  2:3636221/150‑1 (MQ=37)
            gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTcc                                                                                                                >  2:1947581/1‑150 (MQ=37)
              caaGGCGGTAACGCCGCATCCGACATCGAACGCCCGAGCCGGTTGCCTGTTGCGACGCTGGCGCTTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGAAAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                                                                                              <  1:2867923/149‑1 (MQ=11)
                  gCGGTTCCGCCGAATCCGACATCTAACGCGCTTGCGGGTTGCGTTATTGTAATGTGGCGCGTCTTTTGTTGCCTACACGGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                          <  1:3017866/150‑1 (MQ=11)
                  gCGGTTACGCCGCATCCGACATCTCACGCCCAAGCCGGGTGCCTGATGCGACGCTGGCGCGTCTTCTCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTTGGATACGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                          <  1:1704161/150‑1 (MQ=11)
                  gCGGTGACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                          <  2:2674329/150‑1 (MQ=37)
                  gCGGTCCCGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCGTGATGCTACTCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTTGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                          <  1:1704140/150‑1 (MQ=11)
                  gCGGTCACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                          <  1:947937/150‑1 (MQ=255)
                      ttACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTg                                                                                                      >  2:3024443/1‑150 (MQ=17)
                         cgccgcATCCGACATCAAACGGCCCAGCCTGTTGGCTTCTGCTTCGCTGGCGCGTCTTATCAGGCCGACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                                                                                                   <  1:3527403/150‑1 (MQ=11)
                           ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTg                                                                                                  <  1:658019/149‑1 (MQ=37)
                           ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTg                                                                                                  >  2:658019/1‑149 (MQ=37)
                           ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                                                                                                 >  2:1648182/1‑150 (MQ=37)
                             ccATCCGACTTCTTCCTCCGCAGCCGGGTGAGTGATTCTACGCTGTCGAGTCTCTTGAGGACCACCACGCTGTTAAGTGCTGCACCCCGTAGGTTTGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATg                                                                                               <  2:3767914/149‑1 (MQ=2)
                                  cGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACg                                                                                          >  1:1250231/1‑150 (MQ=37)
                                  cGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACg                                                                                          >  2:2815676/1‑150 (MQ=37)
                                   gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGc                                                                                         >  2:2968161/1‑150 (MQ=37)
                                   gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATACAACGTCCGAGCCGGTTGCCTGATGCGACGc                                                                                         >  2:2420793/1‑150 (MQ=37)
                                         tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                   >  2:3188439/1‑150 (MQ=37)
                                         tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAGGCGACGCTGgcgc                                                                                   >  2:2659507/1‑150 (MQ=37)
                                         tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGGAGGGGGGATAAGGCGGTTACGCCGCATCCGACATCCACCGGCCGAGCCGGTTGCCTGCTGCGACGCGGgcgc                                                                                   >  2:2257084/1‑150 (MQ=11)
                                           aCGCCCTAGCCGGTTGCCTGCTGCGACTCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                 <  2:2569193/150‑1 (MQ=11)
                                           aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGACGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGGCCGAGCCGGTTGCCAGATGCGAAGATGGcgcgg                                                                                 >  2:3803324/1‑149 (MQ=11)
                                            cGCCCTAGCGGGTTGCCTGATGCGACGCTGGCGAGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                <  1:167112/150‑1 (MQ=255)
                                            cGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                <  1:771311/150‑1 (MQ=37)
                                             gCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCAACCGACAGCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                               >  1:2497934/1‑150 (MQ=255)
                                              cccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                              >  2:882279/1‑150 (MQ=37)
                                              cccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                              >  2:882327/1‑150 (MQ=21)
                                               cccaGCCGGGTGCCCGATGCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCCGTGAAGTGCGCCACCCCGTAGGTCCGAGAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTa                                                                             <  2:863593/147‑1 (MQ=11)
                                               ccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTa                                                                             <  1:2815676/150‑1 (MQ=37)
                                                   gCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                         <  1:2944800/150‑1 (MQ=37)
                                                   gCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                         <  1:1845773/150‑1 (MQ=37)
                                                    ccGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAgg                                                                        <  1:2968161/150‑1 (MQ=38)
                                                    ccGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATACAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAgg                                                                        <  1:2420793/150‑1 (MQ=38)
                                                     cGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                                       <  1:3188439/150‑1 (MQ=38)
                                                     cGGGTGCCCGATGCGACGCTGGCGCGTCTGATGAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                                       <  2:225814/150‑1 (MQ=11)
                                                       gTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCt                                                                     >  1:487826/1‑150 (MQ=39)
                                                        ttGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAgcatac                                                                    >  1:975051/1‑145 (MQ=17)
                                                            cTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTgg                                                                                      >  1:1481095/1‑128 (MQ=25)
                                                             tGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCTCCACCCCGTAGGGAGGAGAGGGCGGTTACGCCGCATCCGACAACGAAAGACCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTGCAGCg                                                               >  2:2227972/1‑150 (MQ=11)
                                                              gATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGc                                                              <  1:2289446/150‑1 (MQ=39)
                                                              gATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCCTCTTATCAGGCCTACACCga                                                              >  2:3398575/1‑149 (MQ=255)
                                                                tGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtg                                                            >  2:2044442/1‑150 (MQ=39)
                                                                tGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGACATACAACGCtg                                                            >  2:2044441/1‑150 (MQ=11)
                                                                   gACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                                     >  1:3666686/1‑122 (MQ=38)
                                                                   gACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTCCTCCACCCCGTAGGTTGGAAAAGGCGGTTACGCCGCATCCGACATCCAAAGTCCGAGCCGGTTGACTGATGCGACGCTGGCGCGTATTATCAGGCCTACACCGCTGTGa                                                         <  1:1174247/150‑1 (MQ=14)
                                                                        tGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGc                                                    <  2:286648/150‑1 (MQ=39)
                                                                          gcgcGTCCTCTCCGGCCCACACCGCGGTGAAGTGCTCCACCCCCTAGGTTGGATAAGGCGGTTACGCGGCATCCGACATACAACGTCGGTGCAGGTTGCCGGATGCGACTCTGGCGCGTCTTATCAGGCCTACAGCGCTGTGAAGTGCTc                                                  <  1:470451/150‑1 (MQ=14)
                                                                            gcgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGATGAGACGTGCTcaa                                                >  2:1470817/1‑148 (MQ=255)
                                                                            gcgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCACAGCTCGGGAGTGCAccc                                                >  2:1765197/1‑149 (MQ=11)
                                                                              gTCTTATCAGGGCTCGAGCGCTGTGAAGTGCTCCACCCCGTAGGTGGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAcc                                              <  2:540143/150‑1 (MQ=11)
                                                                               tCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCGccc                                             >  2:2230725/1‑150 (MQ=255)
                                                                                 ttATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCAACCCCg                                           >  2:535527/1‑150 (MQ=255)
                                                                                  tATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                          <  1:947925/86‑1 (MQ=255)
                                                                                   aTCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTACACACCTCAGACGTGCTCCACCCCGTa                                         >  2:2142785/1‑150 (MQ=11)
                                                                                    tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCACCGCTGTGACGTGCTCCACCCCGTAg                                        >  2:1384928/1‑150 (MQ=255)
                                                                                    tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGtcg                                        >  2:1385044/1‑148 (MQ=39)
                                                                                    tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGGAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCGGTGACGTGCTCCACCCCGTAg                                        >  2:1384962/1‑150 (MQ=255)
                                                                                      aGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGt                                      >  2:932976/1‑150 (MQ=39)
                                                                                       ggCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTc                                     <  1:1412925/150‑1 (MQ=40)
                                                                                       ggCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTc                                     >  1:3324693/1‑150 (MQ=38)
                                                                                        gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCACCGCTGTGAAGTGCTCCACCCCGAAGGTCg                                    >  2:2794411/1‑150 (MQ=255)
                                                                                         ccTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                            >  1:2216486/1‑61 (MQ=33)
                                                                                          cTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                    <  2:1593753/116‑1 (MQ=255)
                                                                                          cTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGa                                  >  1:1888468/1‑150 (MQ=255)
                                                                                          cTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCGAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                    >  1:1593753/1‑116 (MQ=12)
                                                                                           tACACCGCTGTGAAGTGCTCCCCCCCGTAGGTTGGATCAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAt                                 <  1:1662897/150‑1 (MQ=255)
                                                                                           tACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTACACCGCTCTGACGTGCTCCACCCCGTGGGGCGGAt                                 >  2:2930289/1‑150 (MQ=14)
                                                                                            acacCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCAAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATa                                <  2:1691684/150‑1 (MQ=255)
                                                                                               ccGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                             >  1:43798/1‑150 (MQ=255)
                                                                                                   tgtgAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                >  2:167010/1‑95 (MQ=37)
                                                                                                      gAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAc                      >  2:1577365/1‑150 (MQ=255)
                                                                                                         gTGCTCCACCCCGTAGGTCGGATAAGGCGGTCCAGCCGCCTCCGACAACCACCGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTATTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgcc                   <  2:2094893/150‑1 (MQ=11)
                                                                                                            cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCa                >  1:3262576/1‑150 (MQ=255)
                                                                                                            cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCa                >  2:90966/1‑150 (MQ=255)
                                                                                                            cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCa                >  1:2357632/1‑150 (MQ=255)
                                                                                                            cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCa                >  1:3859053/1‑150 (MQ=255)
                                                                                                            cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCa                >  1:3859085/1‑150 (MQ=255)
                                                                                                            cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTCCGCCGCa                >  2:705596/1‑150 (MQ=255)
                                                                                                            cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAAGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCa                >  1:2357629/1‑150 (MQ=255)
                                                                                                             tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCAt               >  2:21917/1‑150 (MQ=255)
                                                                                                             tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCAt               >  2:51164/1‑150 (MQ=255)
                                                                                                             tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCAGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGATGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCAt               >  2:1587889/1‑150 (MQ=255)
                                                                                                               cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc             >  1:2687094/1‑150 (MQ=255)
                                                                                                               cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAGACGGCGGTTACGCCGCAGcc             >  2:709078/1‑150 (MQ=255)
                                                                                                                   ccGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACa         >  1:3180151/1‑150 (MQ=255)
                                                                                                                   ccGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACa         >  2:3654904/1‑150 (MQ=255)
                                                                                                                    cGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAt        <  1:1648182/150‑1 (MQ=255)
                                                                                                                    cGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAt        >  2:2239901/1‑150 (MQ=255)
                                                                                                                        ggTTGGATACGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGGCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGTCATCTaa    <  1:3722253/150‑1 (MQ=255)
                                                                                                                          ttGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg  <  1:3368570/150‑1 (MQ=39)
                                                                                                                          ttGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg  >  2:3368570/1‑150 (MQ=39)
                                                                                                                           |                                                                                                                                                    
CCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACG  >  NC_000913/2304639‑2304910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: