Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,383,959 A→T 5.4% intergenic (+35/+36) elaD → / ← yfbK protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,383,9590AT 5.4% 726.7 / 24.1 203intergenic (+35/+36)elaD/yfbKprotease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (98/94);  new base T (8/3);  total (106/97)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01

Only 100 of 210 total aligned reads displayed.

CAGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATCTGCTG  >  NC_000913/2383810‑2384108
                                                                                                                                                       |                                                                                                                                                     
cAGAAAGTGTTATTTAGATAAATATGGCGATGCAAATATCACTCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAata                                                                                                                                                       <  1:963298/150‑1 (MQ=255)
    aaTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGCGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCt                                                                                                                                                   <  1:2215039/150‑1 (MQ=255)
    aaTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCt                                                                                                                                                   <  1:549880/150‑1 (MQ=255)
    aaTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCt                                                                                                                                                   <  1:1698363/150‑1 (MQ=255)
    aaTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCt                                                                                                                                                   >  2:3933988/1‑150 (MQ=255)
      ttGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGa                                                                                                                                                 <  1:527208/150‑1 (MQ=255)
      ttGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGa                                                                                                                                                 <  1:2016030/150‑1 (MQ=255)
      ttGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGa                                                                                                                                                 <  1:1133490/150‑1 (MQ=255)
         ttATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTg                                                                                                                                              >  2:1493208/1‑150 (MQ=255)
           aTTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACTGGt                                                                                                                                            <  1:1860211/150‑1 (MQ=255)
            tttAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGCTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTc                                                                                                                                           <  2:2512006/150‑1 (MQ=255)
                 aTAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACa                                                                                                                                      >  2:1693613/1‑150 (MQ=255)
                          gCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCt                                                                                                                             <  2:907343/150‑1 (MQ=255)
                            gATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTaa                                                                                                                           >  1:1056672/1‑150 (MQ=255)
                            gATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTaa                                                                                                                           >  1:2329916/1‑150 (MQ=255)
                             aTGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTaaa                                                                                                                          >  1:1010598/1‑150 (MQ=255)
                             aTGAAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACACACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTaaa                                                                                                                          <  1:2994308/150‑1 (MQ=255)
                               gCAAATATCAATCATTATTATAGAACCTGAGAAATAAAACACTCACAACCCAAAAATAGCGCATCCGGCAAAAGAGTGAGTTAACATCTTTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACa                                                                                                                        <  1:1452570/150‑1 (MQ=255)
                               gCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACACACAACCCAAAAATAGAGCACCCGGCAAAAGAGTGAGTTAACATCATTTTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACa                                                                                                                        <  1:1078344/150‑1 (MQ=255)
                                cAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACa                                                                                                                        <  2:341078/150‑1 (MQ=255)
                                aaattatctatcATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAACATATAGCATCAGTAAAAAGAGTGAGTTAACATCATTGTTATTTAAATCGACCAGTAATCAAATATGCTGAGTGGTCGAAAATAGTTTGCTAAACa                                                                                                                        <  2:1166584/146‑1 (MQ=255)
                                 aaaTATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGt                                                                                                                      >  2:2525404/1‑150 (MQ=255)
                                    tatCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGtcat                                                                                                                   >  2:3652521/1‑150 (MQ=255)
                                    tatCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGtcat                                                                                                                   <  2:1641899/150‑1 (MQ=255)
                                      tcaatcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATAATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGtcatca                                                                                                                 <  2:3827633/150‑1 (MQ=255)
                                         atcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactg                                                                                                              >  1:808681/1‑150 (MQ=255)
                                         atcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactg                                                                                                              >  2:3328395/1‑150 (MQ=255)
                                         atcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactg                                                                                                              >  2:1444560/1‑150 (MQ=255)
                                           caTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactgac                                                                                                            <  1:2978172/150‑1 (MQ=255)
                                            attattATAGAACCTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactgact                                                                                                           <  2:2188391/150‑1 (MQ=255)
                                              tattatAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCActgactga                                                                                                         <  1:2305761/150‑1 (MQ=255)
                                               attatATAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGAt                                                                                                        <  1:2457754/150‑1 (MQ=255)
                                                ttatAGAAACTTAGAAATAAAACACTCCCCACCCAACAATAGAGCATCCGGCAAAAGAGTGAGTTATCATCATTGTTATTTAATTCGACCAGTCTGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATg                                                                                                       <  2:2097746/150‑1 (MQ=255)
                                                ttatAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATg                                                                                                       <  2:2097903/150‑1 (MQ=255)
                                                 tataGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCCTCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgt                                                                                                      <  1:2192877/150‑1 (MQ=255)
                                                 tataGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgt                                                                                                      <  1:1485530/150‑1 (MQ=255)
                                                   taGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtca                                                                                                    <  2:808681/150‑1 (MQ=255)
                                                   taGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtca                                                                                                    >  2:35771/1‑150 (MQ=255)
                                                   taGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtca                                                                                                    >  1:768131/1‑150 (MQ=255)
                                                      aaaCTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagtc                                                                                                 >  2:3180588/1‑150 (MQ=255)
                                                            aGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCa                                                                                           >  2:490058/1‑150 (MQ=255)
                                                                 tAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGGTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGc                                                                                      >  2:3211440/1‑150 (MQ=255)
                                                                    aaCACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTGTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAg                                                                                   >  2:2852005/1‑150 (MQ=255)
                                                                     acacTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGt                                                                                  >  2:2737744/1‑150 (MQ=255)
                                                                     acacTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGt                                                                                  >  1:589672/1‑150 (MQ=255)
                                                                       acTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTc                                                                                >  1:273174/1‑150 (MQ=255)
                                                                       acTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTc                                                                                >  1:2597473/1‑150 (MQ=255)
                                                                         tCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCaa                                                                              <  1:3358770/150‑1 (MQ=255)
                                                                           acaACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATc                                                                            >  2:1032864/1‑150 (MQ=255)
                                                                             aaCCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAg                                                                          <  1:2580591/150‑1 (MQ=255)
                                                                              aCCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAgg                                                                         >  1:2072534/1‑150 (MQ=255)
                                                                                ccAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCg                                                                       <  2:3119692/150‑1 (MQ=255)
                                                                                   aaaaaTAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCAACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGaa                                                                     >  1:384856/1‑150 (MQ=255)
                                                                                    aaaaTAGAGCCTCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAAt                                                                    <  1:70027/150‑1 (MQ=255)
                                                                                      aaTAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATaa                                                                  >  1:38677/1‑150 (MQ=255)
                                                                                      aaTAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATaa                                                                  >  1:2660272/1‑150 (MQ=255)
                                                                                         tAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCGCACCATCCGCCAGTTCAATCAGGCGAATAAAt                                                                >  1:480844/1‑150 (MQ=255)
                                                                                         tAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAAt                                                                >  1:3539941/1‑150 (MQ=255)
                                                                                            agCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTcc                                                             <  1:1262838/150‑1 (MQ=255)
                                                                                             gCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCg                                                            >  2:3402660/1‑150 (MQ=255)
                                                                                              cATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGc                                                           <  2:3779808/150‑1 (MQ=255)
                                                                                               aTCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGcc                                                          <  2:423157/150‑1 (MQ=255)
                                                                                                 ccGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCt                                                        <  2:2373448/150‑1 (MQ=255)
                                                                                                 ccGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCt                                                        >  1:1048521/1‑150 (MQ=255)
                                                                                                 ccGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCt                                                        <  1:3282302/150‑1 (MQ=255)
                                                                                                     cAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTaa                                                    >  1:1895733/1‑150 (MQ=255)
                                                                                                      aaaaGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAAc                                                   >  1:1292662/1‑150 (MQ=255)
                                                                                                       aaaGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAAcc                                                  <  1:730663/150‑1 (MQ=255)
                                                                                                       aaaGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAAcc                                                  <  2:1112900/150‑1 (MQ=255)
                                                                                                           agtgagtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCtgt                                              <  1:3231125/150‑1 (MQ=255)
                                                                                                           agtgagaTAACATCATTGTTAATTAATTCGACCAGTCACCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCtgt                                              <  1:3720478/150‑1 (MQ=255)
                                                                                                            gtgagtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCtgtg                                             >  2:2769075/1‑150 (MQ=255)
                                                                                                               agtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGAt                                          <  2:1399791/150‑1 (MQ=255)
                                                                                                                gtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATc                                         >  1:3084389/1‑150 (MQ=255)
                                                                                                                 ttAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCt                                        >  1:1677137/1‑150 (MQ=255)
                                                                                                                 ttAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCt                                        >  2:2961002/1‑150 (MQ=255)
                                                                                                                 ttAACATCATTGTGAATTAATTCGACCAGTCAGCACATATGCTGACTGGTCGAACATAGGTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACAATCCGCCAGTTCAATCAGGCGAATAAATTCCGCACTGTAACCCTGTGGATCt                                        >  2:225758/1‑150 (MQ=255)
                                                                                                                   aaCATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGAATGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCATGTAACCCTGTGGATCTTc                                      <  2:1308793/150‑1 (MQ=255)
                                                                                                                         atTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCttt                                >  1:2834333/1‑150 (MQ=255)
                                                                                                                         atTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCttt                                >  2:662235/1‑150 (MQ=255)
                                                                                                                            gTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGc                             <  2:2329916/150‑1 (MQ=255)
                                                                                                                                attaattaGACCAGTCAGCACAGATTCTGACTGGTCGAACATAGTTTGCTAAACAGGCATCACTGACTGATGTCAGTCACACCATCCGCCAGTGCAATCAGGCGAATAGATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTGTGCctgc                         >  2:2932212/1‑150 (MQ=255)
                                                                                                                                 ttaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATATTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctgct                        >  1:1546803/1‑150 (MQ=255)
                                                                                                                                 ttaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGACTAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCGTTTGCctgct                        >  2:1856130/1‑150 (MQ=255)
                                                                                                                                 ttaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACAGACAGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctgct                        >  1:31354/1‑150 (MQ=255)
                                                                                                                                    attCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAg                     <  2:3122254/150‑1 (MQ=255)
                                                                                                                                       cGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTCCCCCTTTTGCCTGCTGAGccc                  <  2:2696596/150‑1 (MQ=255)
                                                                                                                                            aGTCAGCATATTTGCTGACTGGTCGAATATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGt             >  1:598234/1‑150 (MQ=255)
                                                                                                                                             gTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGACCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGtt            >  1:360022/1‑150 (MQ=255)
                                                                                                                                              tCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGttt           >  2:1198899/1‑150 (MQ=255)
                                                                                                                                                aGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGa         <  1:490058/150‑1 (MQ=255)
                                                                                                                                                 gCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGAt        >  2:1394232/1‑150 (MQ=255)
                                                                                                                                                 gCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGAt        >  1:3728042/1‑150 (MQ=255)
                                                                                                                                                 gCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGAt        <  2:2978955/150‑1 (MQ=255)
                                                                                                                                                 gCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGACCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGAt        >  1:871294/1‑150 (MQ=255)
                                                                                                                                                  cAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATc       >  2:1785490/1‑150 (MQ=255)
                                                                                                                                                   aaaTATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATct      >  2:2104080/1‑150 (MQ=255)
                                                                                                                                                   aaaTATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATct      >  1:1251824/1‑150 (MQ=255)
                                                                                                                                                       atGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATctgctg  <  1:3652521/150‑1 (MQ=255)
                                                                                                                                                       atGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATctgctg  >  1:1778392/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
CAGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATCTGCTG  >  NC_000913/2383810‑2384108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: