Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 256,449 T→C 5.4% intergenic (+14/‑78) gpt → / → frsA xanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913256,4490TC 5.4% 881.6 / 27.0 274intergenic (+14/‑78)gpt/frsAxanthine phosphoribosyltransferase; xanthine‑guanine phosphoribosyltransferase/fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base T (85/164);  new base C (9/5);  total (105/169)
Fisher's exact test for biased strand distribution p-value = 4.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-01

Only 100 of 277 total aligned reads displayed.

CTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTC  >  NC_000913/256300‑256598
                                                                                                                                                     |                                                                                                                                                     
cTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCt                                                                                                                                                       <  2:1931814/150‑1 (MQ=255)
 tttGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTg                                                                                                                                                      >  2:1496438/1‑150 (MQ=255)
 tttGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTg                                                                                                                                                      >  2:359154/1‑150 (MQ=255)
      cACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACt                                                                                                                                                 >  2:885006/1‑150 (MQ=255)
       aCCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTg                                                                                                                                                >  1:1517049/1‑150 (MQ=255)
            cTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCggg                                                                                                                                           <  2:3583069/150‑1 (MQ=255)
             ttCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGc                                                                                                                                          >  2:2032569/1‑150 (MQ=255)
               cGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgt                                                                                                                                        <  1:2291448/150‑1 (MQ=255)
                gCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgtt                                                                                                                                       <  2:454724/150‑1 (MQ=255)
                 cAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCCGGCAGTGCCAGGCgttg                                                                                                                                      <  2:1866021/150‑1 (MQ=255)
                 cAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCGAATCTTTTCAACGCCTGGCACTGCCGGGCggtg                                                                                                                                      >  1:1808693/1‑150 (MQ=255)
                   aaaCCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCgttgtt                                                                                                                                    <  1:2579095/150‑1 (MQ=255)
                      ccGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCtt                                                                                                                                 <  2:1355695/150‑1 (MQ=255)
                       cGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttt                                                                                                                                >  2:757075/1‑150 (MQ=255)
                       cGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttt                                                                                                                                <  1:464360/150‑1 (MQ=255)
                       cGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCttt                                                                                                                                <  1:3772474/150‑1 (MQ=255)
                          cTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGGTGTTCTTTTTa                                                                                                                             >  2:2344131/1‑150 (MQ=255)
                           tGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTaa                                                                                                                            <  2:1633162/150‑1 (MQ=255)
                                  ccGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAgg                                                                                                                     <  1:906718/150‑1 (MQ=255)
                                     cTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCgg                                                                                                                  <  1:2113551/150‑1 (MQ=255)
                                     cTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCgg                                                                                                                  <  2:2090691/150‑1 (MQ=255)
                                      tGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAAAGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCggg                                                                                                                 <  1:3957301/150‑1 (MQ=255)
                                           gatgaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACa                                                                                                            <  2:1808598/150‑1 (MQ=255)
                                            atgaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCAGGCACTGCCGGGCGTTGTTCTTTTTAACATCAGGCGGGTTACaa                                                                                                           >  2:236446/1‑150 (MQ=255)
                                              gaCTCTGTTGTTGATATCCCGCAAGATACCTGGATGGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCACTCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATa                                                                                                         <  2:1818340/150‑1 (MQ=255)
                                              gaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATa                                                                                                         <  1:1994069/150‑1 (MQ=255)
                                              gaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATa                                                                                                         <  2:1254531/150‑1 (MQ=255)
                                              gaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTAGCTAATCTTTTCAACGCCTGGCACTGCCTGGCTTTGTTCTTTTTAACTTCAGGCGGGTTACAATa                                                                                                         <  2:2620690/150‑1 (MQ=255)
                                              gaCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCATGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCCAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATa                                                                                                         >  1:3847563/1‑150 (MQ=255)
                                               aCTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAAATTCAGGCGGGTTACAATAg                                                                                                        >  1:137349/1‑150 (MQ=255)
                                                cTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGt                                                                                                       <  2:3881031/150‑1 (MQ=255)
                                                cTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGt                                                                                                       <  2:2207068/150‑1 (MQ=255)
                                                cTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGt                                                                                                       <  2:1305714/150‑1 (MQ=255)
                                                 tATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGtt                                                                                                      >  1:3252087/1‑150 (MQ=255)
                                                 tATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGtt                                                                                                      >  1:3042601/1‑150 (MQ=255)
                                                   tgttgtTGATATCCCGCAAGATTCCTGGATTGTAGAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATGTTTTCATCGCGTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTc                                                                                                    <  2:408674/150‑1 (MQ=255)
                                                   tgttgtTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTc                                                                                                    <  2:727786/150‑1 (MQ=255)
                                                    gttgttGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTcc                                                                                                   <  1:1016815/150‑1 (MQ=255)
                                                    gttgttGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTcc                                                                                                   <  1:3278741/150‑1 (MQ=255)
                                                     ttgttgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCa                                                                                                  <  1:2684477/150‑1 (MQ=255)
                                                     ttgtggATCTCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCCATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCa                                                                                                  <  2:2837296/150‑1 (MQ=255)
                                                        ttgATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCagta                                                                                               >  1:552696/1‑150 (MQ=255)
                                                          gATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGGTGTTATTTTTAACTTCAGGCGGGTTACAATAGTTTCCagtaag                                                                                             >  1:3447996/1‑150 (MQ=255)
                                                            tatCCCGCAAGATACCGGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTATTTTTAAATTCAGGCGGGTTTCAAAAGTTTACagtaagta                                                                                           >  1:1861078/1‑150 (MQ=255)
                                                             atCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTAt                                                                                          <  1:3136649/150‑1 (MQ=255)
                                                              tCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTAtt                                                                                         <  2:740050/150‑1 (MQ=255)
                                                                  gCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTgg                                                                                     <  2:3729451/150‑1 (MQ=255)
                                                                        tACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTg                                                                               <  1:204153/150‑1 (MQ=255)
                                                                           cTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCGAATCTTTTCAACGCCTGGCCCTGCCGGGCGGTGTTCTTTTTAACTTCAGGCGGGGTACAAAAGTTTACAGGAAGTATTCGGGAggcggcct                                                                            >  1:3479177/1‑148 (MQ=255)
                                                                            tGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCGAATCTTTTAAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACGTCAGGCGGGTTACAATAGTTTGCAGTAAGTATTACGGGGGCGGCagc                                                                           >  1:2769979/1‑148 (MQ=255)
                                                                             ggATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATcc                                                                          <  1:1418171/150‑1 (MQ=255)
                                                                             ggATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCGAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGGTTCCAGGAAGTATTCTGGAGGCTGCATcc                                                                          >  2:2962190/1‑150 (MQ=255)
                                                                               aTTGAACAGCCGAGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCGAAAATTTTAAACGCCTTGCACTGCCGGGCGTTGTTATTTTTAAAATCAGGCGGTTTACAAAATGTTACAGTAAGTATTCTGGAGGATGCATACAt                                                                        >  2:2695696/1‑150 (MQ=255)
                                                                                 tGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGa                                                                      <  1:719671/150‑1 (MQ=255)
                                                                                    aCAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGacac                                                                   <  1:2528164/150‑1 (MQ=255)
                                                                                    aCAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACAGCCGGGCGTTGTTCTTTTTTAATTCAGGCGGGTTAAAAAAGTTTACAGTAAGTATTATGGGGGCTGCATCCATGacac                                                                   >  1:3528317/1‑150 (MQ=255)
                                                                                    aCAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGACACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGacac                                                                   <  1:2171024/150‑1 (MQ=255)
                                                                                     cAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGAcaca                                                                  <  1:3304573/150‑1 (MQ=255)
                                                                                     cAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGAcaca                                                                  <  1:3373091/150‑1 (MQ=255)
                                                                                     cAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGAcaca                                                                  <  1:2497954/150‑1 (MQ=255)
                                                                                      aGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAg                                                                 <  1:158131/150‑1 (MQ=255)
                                                                                      aGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAg                                                                 <  2:2787956/150‑1 (MQ=255)
                                                                                        ccGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGc                                                               >  2:1102180/1‑150 (MQ=255)
                                                                                           tGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCaaa                                                            <  2:2254745/150‑1 (MQ=255)
                                                                                           tGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCAAGACACAGGCaaa                                                            >  2:3696071/1‑150 (MQ=255)
                                                                                            gggATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAAc                                                           <  1:1252139/150‑1 (MQ=255)
                                                                                              gATATGGGCGTCGCATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCt                                                         <  1:1350232/150‑1 (MQ=255)
                                                                                                  tGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGc                                                     <  2:1517049/150‑1 (MQ=255)
                                                                                                      cgtcgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGaaa                                                 <  2:2945196/150‑1 (MQ=255)
                                                                                                      cgtcgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGaaa                                                 <  1:1042236/150‑1 (MQ=255)
                                                                                                       gtcgtATTCGTCCCGCCAATCTCCGGTCGATAATCTTTTCCACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGTGGCTGCATCCATGACACAGGCAAACCGGAGCGAAAc                                                <  1:569087/150‑1 (MQ=255)
                                                                                                       gtcgtATTCGTCCCGCAAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTATTTTTAACTTCAGGCGGGTTACATTTGTTTCCTGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAAc                                                <  1:790918/150‑1 (MQ=255)
                                                                                                        tcgtATTCGTCCCGCCCATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGGTGTTTTTAACTTCAGGCGGGTTACAATAGATTCCCTTAAGTATTCTGGCGGCGGCATCCAGGACACAGGCAAACCTGAGCgtaccc                                               >  2:2746518/1‑145 (MQ=255)
                                                                                                         cgtATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAAccc                                              <  2:1056610/150‑1 (MQ=255)
                                                                                                           tATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTg                                            <  2:100416/150‑1 (MQ=255)
                                                                                                           tATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTg                                            <  2:3486087/150‑1 (MQ=255)
                                                                                                            aTTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGt                                           <  1:3223334/150‑1 (MQ=255)
                                                                                                             ttCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGtt                                          <  2:2559008/150‑1 (MQ=255)
                                                                                                             ttCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGtt                                          <  2:3239290/150‑1 (MQ=255)
                                                                                                                 tCCCGCCACTCTCCGGTCGCTAATCGTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCaaa                                      >  2:566566/1‑150 (MQ=255)
                                                                                                                   ccGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAAcc                                    <  1:2721547/150‑1 (MQ=255)
                                                                                                                          tctcCGGTCGCTAATCTTTTCAACGCCAGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCgtt                             >  2:1551700/1‑150 (MQ=255)
                                                                                                                          tctcCGGTCGCTAAACTTTTAAAAGCCTGGCACTGCCGGGCGGTGTTATTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCAATGACACAGGCAAACCTGAGCGAAACCCAGTTAAAACCCCGCgtt                             >  2:3424052/1‑150 (MQ=255)
                                                                                                                            tcCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTaa                           <  2:3098266/150‑1 (MQ=255)
                                                                                                                             ccGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTaaa                          <  2:1194867/150‑1 (MQ=255)
                                                                                                                              cGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAAc                         >  2:3300313/1‑150 (MQ=255)
                                                                                                                                     tAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGa                  >  2:1465100/1‑150 (MQ=255)
                                                                                                                                     tAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGa                  >  2:849673/1‑150 (MQ=255)
                                                                                                                                       aTCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGGTACAATAGTTTCCAGTAAGTATTATGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTGTAAACATCCTGaaa                >  2:2353062/1‑150 (MQ=255)
                                                                                                                                           tttCAACGCCTGGCACTGCCGGGCGTTGTTATTTTTAACTTCAGGCGGGTTACAAAAGTTTACAGTAAGTATTATGGCGGCTGCAACCAAGAAACACGCAAACCAGAGCGAAACCCAGTTCAAACCCCCCTGTAAACATACAGAAAccac            >  1:2411164/1‑148 (MQ=255)
                                                                                                                                            aacAACGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAAATTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATACATGAAACAGGCAAACCTGAGCGAAACCCAGTTCAAACCCCGCTTTAAACATCCTGAAACCTCg           >  1:165665/3‑150 (MQ=255)
                                                                                                                                            aacAACGCCCGGCAGTGCCAGGCGTTGTTATTTTTAAATTCAGGCGGGTTACAATATTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCg           >  1:3691834/3‑150 (MQ=255)
                                                                                                                                             tCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTTACTTCTGGCGGGTTTCAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGTCACAGGCTAAGCTGAGCGAAACCCTGTTCAAACCCCGCTTTAATCATCCTGAAACCTCGa          <  2:3628061/150‑1 (MQ=255)
                                                                                                                                             tCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGa          <  1:3086607/150‑1 (MQ=255)
                                                                                                                                               aaCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACg        <  2:3174559/150‑1 (MQ=255)
                                                                                                                                               aaCGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATCGTTTCCAGTAAGGATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAATCCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGCCg        >  2:2505557/1‑150 (MQ=255)
                                                                                                                                                aCGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGc       >  2:102479/1‑150 (MQ=255)
                                                                                                                                                 cGCCCGGCAGTGCCAGGCGTTGTTCTTTTTAAATTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTAAAACCCCGCGTTAAACATCCTGAAACCACGACGCt      >  1:1202181/1‑150 (MQ=255)
                                                                                                                                                  gCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTa     <  1:2943326/150‑1 (MQ=255)
                                                                                                                                                     tGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTc  >  2:1766819/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAATCTTTTCAACGCCTGGCACTGCCGGGCGTTGTTCTTTTTAACTTCAGGCGGGTTACAATAGTTTCCAGTAAGTATTCTGGAGGCTGCATCCATGACACAGGCAAACCTGAGCGAAACCCTGTTCAAACCCCGCTTTAAACATCCTGAAACCTCGACGCTAGTC  >  NC_000913/256300‑256598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: