Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,880 A→C 5.8% intergenic (+97/+55) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8800AC 5.8% 604.8 / 3.0 197intergenic (+97/+55)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (57/122);  new base C (13/3);  total (70/127)
Fisher's exact test for biased strand distribution p-value = 1.50e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

CTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGT  >  NC_000913/3504732‑3505028
                                                                                                                                                    |                                                                                                                                                    
 ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                                    <  1:1719345/150‑1 (MQ=255)
 ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                                    <  2:1472462/150‑1 (MQ=255)
  tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                                                   <  2:2259066/150‑1 (MQ=255)
  tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                                                   <  2:1027012/150‑1 (MQ=255)
  tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                                                   <  2:3403459/150‑1 (MQ=255)
   ctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAgg                                                                                                                                                  <  2:1768371/150‑1 (MQ=255)
     gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCGCACCCCCAAgggg                                                                                                                                                >  1:3554078/1‑150 (MQ=255)
     gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGATGGCGCGTTGAATTTTACATCCCGTACGTTCACCACACCCAAACCCTCTCCCCCAAgggg                                                                                                                                                >  1:3927733/1‑150 (MQ=39)
          gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCTTTGGGGAGAgg                                                                                                                                           <  2:3400341/150‑1 (MQ=255)
           ccAATCGCATCACCTTAACTATTGATAATAAAAGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                                          <  1:1724944/150‑1 (MQ=255)
             aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGAAACCAAACCCACACCCCAAAAGGGCGAGGGGa                                                                                                                                        >  2:467180/1‑150 (MQ=38)
                  cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                                                   <  1:812239/150‑1 (MQ=255)
                       ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                                              <  1:2458518/150‑1 (MQ=255)
                       ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                                              <  1:2085597/150‑1 (MQ=255)
                        cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                                             <  2:1459429/150‑1 (MQ=255)
                        cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCCAACGGGCGCGGGGCCCGGTCACTAc                                                                                                                             >  1:3383680/1‑150 (MQ=38)
                        cTTAACTAGTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                                             <  1:622783/150‑1 (MQ=255)
                         ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                                            <  1:1909767/150‑1 (MQ=255)
                            aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                                         <  2:45711/150‑1 (MQ=255)
                            aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                                         <  1:1415354/150‑1 (MQ=255)
                            aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                                         <  1:2640332/150‑1 (MQ=255)
                              tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAACCCACAACCCAAACGGGGGAGGGGGCCGGTCAGTACGGACtt                                                                                                                       >  2:3752528/1‑150 (MQ=37)
                              tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCACACCCCAAAGGGGCGAGGGGACCGTTCAGTAAGGaaat                                                                                                                       >  2:1960951/1‑148 (MQ=255)
                                  gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGc                                                                                                                   <  2:2802092/150‑1 (MQ=255)
                                  gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGc                                                                                                                   <  2:2332252/150‑1 (MQ=255)
                                   ataataAATGACATTAACCGTAGGCCGGTTAAGAGGCGCCAGCATCGCATCCGGCGATGCTGGCGAGTTGAATTTTTCTTCCCGTACGTTCCCCTCACCCTATCCCTCTCCCCAAATGGGCGAGGGGACCGTTCAGTTCTGAATTTAGCt                                                                                                                  <  2:161011/150‑1 (MQ=255)
                                   ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                                                  <  1:1568131/150‑1 (MQ=255)
                                   ataataAAAGACATTAACCGGAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                                                  <  1:2033427/150‑1 (MQ=255)
                                      ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                                               <  1:3115182/150‑1 (MQ=255)
                                       taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                                              >  2:2903352/1‑150 (MQ=255)
                                          aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGt                                                                                                           <  2:2051106/150‑1 (MQ=255)
                                          aaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGt                                                                                                           <  2:3656415/150‑1 (MQ=255)
                                           aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                                          <  2:1515232/150‑1 (MQ=255)
                                           aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                                          <  1:1799793/150‑1 (MQ=255)
                                            gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCAACCAAACCCACAACCCCAAAGGGCGGGGGGACCCGTCCGTAACGAAATTATCGTGTCGGTGc                                                                                                         >  2:1049679/1‑150 (MQ=21)
                                            gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCACCACACCCAAAACCACACCCCCAACCGGCGAGGGGACCGGTCACAAAGGAAATTAGCTTGACGGTGc                                                                                                         >  1:2885687/1‑150 (MQ=25)
                                            gACATTAACCGTAGGCCGGATAAGATGCGCCAGCAACGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACAACCCAAAGGGGCGAGGGGACCCTTCAGTACGGAAATTATCTTTAAGGTga                                                                                                         >  1:3274163/1‑149 (MQ=37)
                                                  caCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                                   <  2:1714873/149‑1 (MQ=255)
                                                  aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                                                   <  2:768159/150‑1 (MQ=255)
                                                     cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCTGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTATCCCTCTCCCCATAGGGGGGAGGGGACCGTTCATTACGGTATTTATATTGACGGTTCTATCCAGct                                                                                                <  1:3253734/150‑2 (MQ=38)
                                                     cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAAACCACACCCCAAACGGGCGCGGGGACCCTTCAGTAACGAAATTATCTTGACCGTGCAAACCAGCa                                                                                                >  2:1188478/1‑150 (MQ=25)
                                                          gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                           <  1:2933885/150‑1 (MQ=255)
                                                          gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                                           <  1:454276/150‑1 (MQ=255)
                                                           ccGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCTAAACCTCAACCCCAAGGGGGGAGGGGACCGTTAAGTACGGGAGTTATCGTGAAGGTTCTATCCCTCATTAAAt                                                                                          >  2:90034/1‑150 (MQ=255)
                                                              gATAAGAGGCGCCAGCATCGCATCCGGCGAGGCTGGCGCGTTGAATTGTACATCCCGTACGGTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                                       <  2:68330/150‑1 (MQ=39)
                                                               aaaaGATGCGCCACCATCGCAACCGGCCATGCACCCGCCATCAAAATTACACCCCCCACCTTCCCCACACCCTAACCCACACCCCAAACGGGCGAGGGGACCGTTCAGTACGGAAATTAGCACCACGGTGCTATCCACCAATAAATACTg                                                                                      <  2:1810291/148‑1 (MQ=255)
                                                                   gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGATTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                                                  <  1:1661677/150‑1 (MQ=255)
                                                                   gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                                                  <  2:1542571/150‑1 (MQ=255)
                                                                   gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                                                  <  1:438024/150‑1 (MQ=255)
                                                                       cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAct                                                                              <  2:2228881/150‑1 (MQ=255)
                                                                           aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCa                                                                          <  1:3659023/150‑1 (MQ=255)
                                                                            gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                                         <  1:699245/150‑1 (MQ=255)
                                                                            gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                                         <  1:2352388/150‑1 (MQ=255)
                                                                             catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAcc                                                                        >  2:2503359/1‑149 (MQ=255)
                                                                             catcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCACACCCCAAAGGGGCGAGGGGACCGGTCAGTACCGAATTTAGCTGGACGGGGCGATCCAGCATTAAAGACTGGCAATACTCTCACg                                                                        >  2:2209963/1‑150 (MQ=38)
                                                                                 gccggatgCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGGCCGTTCAGTACGGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                                                    >  1:3740622/8‑150 (MQ=255)
                                                                                     ccGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATcc                                                                >  2:3951237/1‑150 (MQ=255)
                                                                                     ccGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATcc                                                                <  1:2534131/150‑1 (MQ=255)
                                                                                      cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                                               <  2:1960655/150‑1 (MQ=255)
                                                                                      cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                                               <  2:1637397/150‑1 (MQ=255)
                                                                                      cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                                               >  2:648708/1‑150 (MQ=255)
                                                                                      cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                                               <  2:2523378/150‑1 (MQ=255)
                                                                                      cGGCGATGCTGGCGCGTGGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCGTGACGGTGCTCTCCAGCAGTAAAAACTGGCAATACTCTCACGCACAATCCg                                                               >  2:2639695/1‑150 (MQ=38)
                                                                                       ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTAACCCCAAAGGGGCCAGGGGACCGTTCAGGACGGAAATTAGCTTTAAGGGGCTAACCCGCCTTAAATACATGCCAAACTCTCACCCAAAAGCCgc                                                              >  2:3134943/1‑150 (MQ=255)
                                                                                          gATGCTGGCGCGTTGAATTATACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACCGAATATAGCTTGACGGTGCTATCCAGCATTAAAAACTGGCAATACTCTCACGCAAAATCCgcagc                                                           >  2:2547650/1‑150 (MQ=39)
                                                                                                 gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtct                                                    <  1:789959/150‑1 (MQ=255)
                                                                                                 gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtct                                                    >  2:2615189/1‑150 (MQ=255)
                                                                                                 gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACACTCACGCAAAATCCGCAGCAACGtct                                                    >  2:2557120/1‑150 (MQ=255)
                                                                                                 gcgcGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCTCTCCCCCAAGGGGCGCGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAATAAATAAGGGCAATAAAATCAAGCAAAATCCGCAGCACCGtct                                                    >  2:3823018/1‑150 (MQ=255)
                                                                                                 gcgcGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACAACCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAGTTAGCTTGACGGTGCTAACCAGCATTAAATAATGGCAAAACAATAACGCAAAATCCGCAGCACCGtct                                                    >  2:1951158/1‑150 (MQ=255)
                                                                                                     gTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTc                                                <  2:3726081/150‑1 (MQ=255)
                                                                                                      ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa                                               <  1:1052784/150‑1 (MQ=255)
                                                                                                      ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACACTCACGCAAAATCCGCAGCACCGTCTCTTCa                                               >  2:3302616/1‑150 (MQ=255)
                                                                                                           ttttACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGAACGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCGTCACCGCt                                          >  2:3236790/1‑150 (MQ=255)
                                                                                                              tACATCCCGTACGTTCCCCACACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCTAACCAGCATTAAAAACTGGCAAAACTCTCACGCAAAAACCGCAGCACCGTCTCTTCACCgcggcg                                       >  2:613950/1‑150 (MQ=38)
                                                                                                                    ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg                                 <  1:2686166/150‑1 (MQ=255)
                                                                                                                    ccGTACGTTCCCCTCACCCGAACCCTCTCCCCAAATGGGCGAGGGGACCCTTCAGTACCGAATTTAGCTTGACGGGGCTATCCAGCATTAAATACTGGCCATACTCTCACGCAAAAACCGCAGCACCGTCTCGTCACCGCTGCGATTCgg                                 >  2:1965714/1‑150 (MQ=255)
                                                                                                                      gTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt                               <  1:574877/150‑1 (MQ=255)
                                                                                                                       tACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGtt                              >  2:835312/1‑150 (MQ=255)
                                                                                                                          ggtCCGCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGTCGGTGCTATCAATCATTATATACTGGCAATACTCTCACGCAATCTCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt                           <  2:2078064/148‑1 (MQ=38)
                                                                                                                                cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt                     >  1:1946162/1‑150 (MQ=255)
                                                                                                                                cTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAAt                     <  2:716175/150‑1 (MQ=255)
                                                                                                                                       tAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACaa              >  1:686897/1‑150 (MQ=255)
                                                                                                                                       tAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACaa              >  1:1184680/1‑150 (MQ=255)
                                                                                                                                       tAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCATCACCGCTGCGATTCGGGTTAATGCGAATCGCACaa              >  2:3914921/1‑150 (MQ=255)
                                                                                                                                       tAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAGTAAATACTGGCAATACTCTCACGCTAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACaa              >  2:2393925/1‑150 (MQ=255)
                                                                                                                                        aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAAt             <  2:3672849/150‑1 (MQ=255)
                                                                                                                                        aaCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAAt             <  1:2961182/150‑1 (MQ=255)
                                                                                                                                           ccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGtt          >  2:1708924/1‑150 (MQ=255)
                                                                                                                                           ccTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGGTAATGCGAATCGCACACTGtt          >  1:976146/1‑150 (MQ=255)
                                                                                                                                            ctctCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTc         <  2:1071785/150‑1 (MQ=255)
                                                                                                                                             tctcCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCt        <  1:3182414/150‑1 (MQ=255)
                                                                                                                                               tcCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGa      <  1:3450225/150‑1 (MQ=255)
                                                                                                                                               tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGTACAATGTTCTGa      >  2:1377224/1‑150 (MQ=255)
                                                                                                                                               tcCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGa      <  1:3572208/150‑1 (MQ=255)
                                                                                                                                               gccccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGa      <  1:3467472/149‑1 (MQ=255)
                                                                                                                                                 cccAAAGGGGCGAGGGGACCGTTCAGTACGGACTTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCACAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTGAATGCGAATCGCACAATGTTCTGAtg    >  2:592155/1‑149 (MQ=255)
                                                                                                                                                  ccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtg   >  2:3855987/1‑150 (MQ=255)
                                                                                                                                                  ccAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtg   >  2:3439158/1‑150 (MQ=255)
                                                                                                                                                   cAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATtgt  <  2:582312/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCGAATCGCACAATGTTCTGATTGT  >  NC_000913/3504732‑3505028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: