Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 66,534 T→G 6.8% K6T (AAA→ACA)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091366,5340TG 6.8% 2065.1 / 37.3 654K6T (AAA→ACA) araDL‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (222/374);  new base G (56/2);  total (278/376)
Fisher's exact test for biased strand distribution p-value = 3.07e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 677 total aligned reads displayed.

TATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAAT  >  NC_000913/66385‑66683
                                                                                                                                                      |                                                                                                                                                     
 atCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑ttt                                                                                                                                                      <  2:1561355/150‑1 (MQ=255)
  tCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTg                                                                                                                                                     <  2:7386808/150‑1 (MQ=255)
  tCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTg                                                                                                                                                     >  1:2453333/1‑150 (MQ=255)
   cGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTga                                                                                                                                                    <  1:9321082/150‑1 (MQ=255)
    gTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTgag                                                                                                                                                   >  1:2004047/1‑150 (MQ=255)
      cAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGAt                                                                                                                                                 <  1:8385852/150‑1 (MQ=255)
      cAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGAt                                                                                                                                                 <  2:8107796/150‑1 (MQ=255)
      cAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGAt                                                                                                                                                 <  1:4081667/150‑1 (MQ=255)
        gCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGAtct                                                                                                                                               >  2:1053441/1‑150 (MQ=255)
         cGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGAtctt                                                                                                                                              >  1:7532096/1‑150 (MQ=255)
         cGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCACCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGAtctt                                                                                                                                              <  1:8691296/150‑1 (MQ=255)
          ggTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCACATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TGGGAGAtcttc                                                                                                                                             >  1:8561948/1‑150 (MQ=255)
           gTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCCTGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGAtcttct                                                                                                                                            <  2:7895701/150‑1 (MQ=255)
             cATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTaa                                                                                                                                          <  1:5556958/150‑1 (MQ=255)
             cATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTaa                                                                                                                                          <  1:733616/150‑1 (MQ=255)
               tGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCCGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTCATACCTGGCG‑GGGGAGATCTTCTAACa                                                                                                                                        >  1:4832408/1‑150 (MQ=255)
                   gCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGtt                                                                                                                                    <  2:5658224/150‑1 (MQ=255)
                    cTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTg                                                                                                                                   >  1:7604933/1‑150 (MQ=255)
                     tGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGa                                                                                                                                  <  2:1196338/150‑1 (MQ=255)
                      gTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGAc                                                                                                                                 <  2:2157792/150‑1 (MQ=255)
                           cGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCtt                                                                                                                            <  2:4846070/150‑1 (MQ=255)
                            gACGCCGGAAGGTTTGATCACAACGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTc                                                                                                                           <  1:915315/150‑1 (MQ=255)
                                ccGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGc                                                                                                                       <  2:8888271/150‑1 (MQ=255)
                                 cGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGcc                                                                                                                      >  2:5221074/1‑150 (MQ=255)
                                  ggAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCg                                                                                                                     <  2:7093321/150‑1 (MQ=255)
                                  ggAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCg                                                                                                                     <  1:2905911/150‑1 (MQ=255)
                                  ggAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TGGGAGATCTTCTAACATGTTGACTCCTTCGTGCCg                                                                                                                     >  1:6305036/1‑150 (MQ=255)
                                   gAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCgg                                                                                                                    >  1:620431/1‑150 (MQ=255)
                                    aaGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCgg                                                                                                                    <  2:3203775/150‑1 (MQ=255)
                                       gTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATgc                                                                                                                >  2:4651403/1‑150 (MQ=255)
                                        tttGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGGTGTGTTTTGGCAGCGCCAGGGTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATgcg                                                                                                               >  2:5424563/1‑150 (MQ=255)
                                         ttGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATgcgc                                                                                                              <  1:7934750/150‑1 (MQ=255)
                                         ttGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCAGGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCATCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGACGGATgcgc                                                                                                              >  2:1798944/1‑150 (MQ=255)
                                           gATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTGGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCtt                                                                                                            >  1:100896/1‑150 (MQ=255)
                                            aTCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCttt                                                                                                           <  1:6526395/150‑1 (MQ=255)
                                            aTCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCttt                                                                                                           <  1:710722/150‑1 (MQ=255)
                                             tCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTg                                                                                                          <  1:5167487/150‑1 (MQ=255)
                                                  aaGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTAt                                                                                                     >  2:2796547/1‑150 (MQ=255)
                                                     aCGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCg                                                                                                  <  2:856034/150‑1 (MQ=255)
                                                       gccgcGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGc                                                                                                >  2:9604798/1‑150 (MQ=255)
                                                       gccgcGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TGTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGc                                                                                                >  1:215761/1‑150 (MQ=255)
                                                         cgcgCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCt                                                                                              <  1:9638287/150‑1 (MQ=255)
                                                             cTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACaa                                                                                          <  1:5969154/150‑1 (MQ=255)
                                                              tCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGtt                                                                                                                                    >  2:3485420/1‑107 (MQ=255)
                                                               cgcgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACaaaa                                                                                        <  2:6252025/150‑1 (MQ=255)
                                                               cgcgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACaaaa                                                                                        <  1:1523134/150‑1 (MQ=255)
                                                               cgcgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TGTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACaaaa                                                                                        >  1:3828198/1‑150 (MQ=255)
                                                                gcgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAAt                                                                                       <  2:3038393/150‑1 (MQ=255)
                                                                 cgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATc                                                                                      >  2:5989103/1‑150 (MQ=255)
                                                                 cgATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATc                                                                                      <  1:1893885/150‑1 (MQ=255)
                                                                  gATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCg                                                                                     >  2:8620218/1‑150 (MQ=255)
                                                                      aaCGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGc                                                                                 <  2:6049651/150‑1 (MQ=255)
                                                                        cGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCgt                                                                               <  2:6948228/150‑1 (MQ=255)
                                                                          gcgcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCgtgt                                                                             <  2:446879/150‑1 (MQ=255)
                                                                            gcTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTATTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAg                                                                           <  1:2776822/150‑1 (MQ=255)
                                                                                aCGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCt                                                                       <  2:4049095/150‑1 (MQ=255)
                                                                                      ccccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAg                                                                 <  2:4136096/150‑1 (MQ=255)
                                                                                       cccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGa                                                                <  1:2684985/150‑1 (MQ=255)
                                                                                       cccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGa                                                                >  2:2992202/1‑150 (MQ=255)
                                                                                       cccATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGa                                                                <  2:1897546/150‑1 (MQ=255)
                                                                                            gtgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAACTCGCAGCGTGTAGGCCTGATAAGACgcgc                                                           <  2:255429/150‑1 (MQ=255)
                                                                                             tgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAATATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGAcgcgac                                                          >  2:1318182/1‑148 (MQ=255)
                                                                                             tgAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACACAATCGCAGCGTGTAGGCCTGATAAGACGCGcc                                                          >  2:6406729/1‑150 (MQ=255)
                                                                                                  gTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGt                                                     <  2:8299342/150‑1 (MQ=255)
                                                                                                   tGACCATGTTGTGTTTTGGCAGCGCCAGGTTGTCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTc                                                    <  1:3226628/150‑1 (MQ=255)
                                                                                                   tGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCACAGCGTGTAGGCCTGATAAGACGCGCCAGCGTc                                                    <  2:3883466/150‑1 (MQ=255)
                                                                                                    gACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCg                                                   <  1:8299262/150‑1 (MQ=255)
                                                                                                       cAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTGGAGATCTTCTAACAAGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCAt                                                >  1:5071955/1‑150 (MQ=255)
                                                                                                           ttGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg                                            >  2:2435333/1‑150 (MQ=255)
                                                                                                            tgtgTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCAACAAAATCGCAGCGTGTAGGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                           <  2:784412/150‑1 (MQ=37)
                                                                                                              tgtTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGt                                         <  1:4612983/150‑1 (MQ=255)
                                                                                                              tgtTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACAAGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAAGACGCGCCAGCGTCGCATCAGGCGt                                         >  1:8890146/1‑150 (MQ=255)
                                                                                                                ttttGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTg                                       >  2:9326134/1‑150 (MQ=255)
                                                                                                                ttttGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTg                                       >  2:8816289/1‑150 (MQ=255)
                                                                                                                ttttGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TGTGAGATCTTCTAACATTTTGACTCCGTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATAGCAGCGTGTAGGCCAGATAATACGCGCCAGCGTCGCATCAGGCGGTg                                       >  1:664494/1‑150 (MQ=25)
                                                                                                                   gggCCGCGCCAGGGTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGTCTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGTTAAGACGCGCCAGCGTCGCATCAGGCGTTGAAt                                    <  2:6669660/149‑1 (MQ=255)
                                                                                                                     gCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAAAAAGTTGAATCCATAGTGCCGGAGGCGCTTTGCTTATCCCGCCTACAAAAACGCAGCGGGGAGGCCGGATAAGACGCGCCAGCGGCGCATCAGGCGGTGAATGc                                  >  1:2334134/1‑150 (MQ=255)
                                                                                                                     gCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TGTGAGATATTCTAACATGTTGAATACATAGTGCCGGATGCGCGTTGCTTATACGGCCTACAAAATCGCAGCGGGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGGTGAATGc                                  >  1:6050893/1‑150 (MQ=18)
                                                                                                                      cAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGTTGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGcc                                 <  1:6871798/150‑1 (MQ=255)
                                                                                                                       aGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCg                                <  2:7604044/150‑1 (MQ=255)
                                                                                                                        gcgcCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCgg                               <  1:1188074/150‑1 (MQ=255)
                                                                                                                         cgcCAGGTTGGCTTCTAATACCTGGCG‑TGGGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGGTGCGCTTTGCATATCCGGCCTAACAAATCGCAGCGGGTTGGCCGGTGAATACGCGCCCGCGGCGCATCAGGCGTTGGATGCCggg                              >  1:5823826/1‑149 (MQ=255)
                                                                                                                          gcCAGGTTGGCTTCTAATACCTGGCG‑TGTGAGATCTTATAAAAAGTTTAAAACATAGTTCCCGATGCGCTTTGCTTATCCGGCCTAAAAAAACCCAGCGTGGAGGCCGGATAAGAAGCGCCATCGGCGCAGAAGGCGTTGAATTCCGGAt                             >  1:7786314/1‑150 (MQ=12)
                                                                                                                           ccAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATg                            <  1:1052634/150‑1 (MQ=255)
                                                                                                                                 tGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGACTGCCGGATGCGCttt                      <  2:4234112/150‑1 (MQ=255)
                                                                                                                                   gCTTCTAATCCCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGc                    <  2:9436046/150‑1 (MQ=255)
                                                                                                                                     ttCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCtt                  >  2:9086433/1‑150 (MQ=255)
                                                                                                                                         aaTACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATcc              <  2:3652818/150‑1 (MQ=255)
                                                                                                                                           tcccTGGCG‑TTTGAGATCTTCTAACATGTGGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCgg            <  2:5024540/148‑1 (MQ=255)
                                                                                                                                            aCCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGc           <  2:7967774/150‑1 (MQ=255)
                                                                                                                                            aCCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGc           <  1:5325068/150‑1 (MQ=255)
                                                                                                                                            aCCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGc           <  2:9129643/150‑1 (MQ=255)
                                                                                                                                            aCCTGGCG‑TTAGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGc           <  1:4361427/150‑1 (MQ=255)
                                                                                                                                             ccTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGcc          <  1:5732727/150‑1 (MQ=255)
                                                                                                                                               tGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTa        >  2:7283095/1‑150 (MQ=255)
                                                                                                                                               tGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGGGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCGTTGCTTATCCGGCCTa        >  2:3304694/1‑150 (MQ=255)
                                                                                                                                                   g‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACaaa    >  1:3697899/1‑150 (MQ=255)
                                                                                                                                                   g‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACaaa    >  2:5792880/1‑150 (MQ=255)
                                                                                                                                                     tttGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACaaaa   >  1:4175835/1‑150 (MQ=255)
                                                                                                                                                     tttGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACaaaa   <  2:4194183/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCG‑TTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAAT  >  NC_000913/66385‑66683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑