Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,022 T→G 8.4% intergenic (+5/‑193) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0220TG 8.4% 1325.5 / 31.1 464intergenic (+5/‑193)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (120/293);  new base G (47/3);  total (168/296)
Fisher's exact test for biased strand distribution p-value = 9.50e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 481 total aligned reads displayed.

TCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCAC  >  NC_000913/3780873‑3781168
                                                                                                                                                     |                                                                                                                                                  
 cTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGttt                                                                                                                                                   <  2:5092365/150‑1 (MQ=255)
  tGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTg                                                                                                                                                  >  2:1835519/1‑150 (MQ=255)
    aTCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTtcg                                                                                                                                                >  2:896201/1‑150 (MQ=255)
        aaaaaGAGATGACAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGcccc                                                                                                                                            <  1:8343152/150‑1 (MQ=255)
        aaaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGTGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGcccc                                                                                                                                            >  1:5957576/1‑150 (MQ=255)
        aaaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGcccc                                                                                                                                            >  1:5395250/1‑150 (MQ=255)
         aaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGccccc                                                                                                                                           <  1:9154465/150‑1 (MQ=255)
                 tGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCTTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCAacccc                                                                                                                                   >  2:3570935/1‑148 (MQ=255)
                 tGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGATAGGGAATGCGGCATAGTCGTGTGCGCccccccccccga                                                                                                                                   >  1:6581817/1‑148 (MQ=255)
                  gAAAGGGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTaa                                                                                                                                  <  1:8672783/150‑1 (MQ=255)
                   aaaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAAc                                                                                                                                 >  2:5665634/1‑150 (MQ=255)
                         gCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCATGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCATCACCCTAACCCtctc                                                                                                                           <  1:4905988/150‑1 (MQ=255)
                         gCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCtctc                                                                                                                           <  2:543999/150‑1 (MQ=255)
                          cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTcc                                                                                                                          <  2:1130040/150‑1 (MQ=255)
                           gATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCAAACCCACAccc                                                                                                                         >  1:1514042/1‑150 (MQ=255)
                               aCGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                     <  2:4730298/150‑1 (MQ=255)
                                cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                                                    <  2:7909000/150‑1 (MQ=255)
                                cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCCAACCCGCACCCCCAgg                                                                                                                    >  2:6745616/1‑150 (MQ=255)
                                 gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCAAACCCACACCCGCAggg                                                                                                                   >  2:607703/1‑150 (MQ=255)
                                 gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTCGCCCCCCCCCCCCAAACCACAACCCCAggg                                                                                                                   >  2:6607098/1‑150 (MQ=255)
                                    gACTGGCGCGAAATCGATCAGCGAAATGACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGgaga                                                                                                                <  2:6385474/150‑1 (MQ=255)
                                     aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                               <  1:7227810/150‑1 (MQ=255)
                                     aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                               <  1:6170460/150‑1 (MQ=255)
                                     aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCTCGCCCGCAGGGggag                                                                                                               >  2:9677435/1‑150 (MQ=255)
                                      cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                              <  1:3341161/150‑1 (MQ=255)
                                      cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGATGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                              <  2:9265704/150‑1 (MQ=255)
                                       tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGTGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                             <  1:2446516/150‑1 (MQ=255)
                                       tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGTGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                             <  1:6897070/150‑1 (MQ=255)
                                       tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAggg                                                                                                             <  2:5183499/150‑1 (MQ=255)
                                       tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCCCCCCCCCCCCCAAAACCACACCCAAAGgggggggg                                                                                                             >  2:1958803/1‑150 (MQ=255)
                                       gggCGCGAAATCGATCAGCGACATTCCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGGTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                             <  2:1423206/149‑1 (MQ=255)
                                         gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCCAACCCACAACCACAGGGCggggggg                                                                                                           >  1:1931259/1‑149 (MQ=255)
                                           gcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACAACCCAAACCACTCCCACACGGGGGGGGGccc                                                                                                         >  2:160707/1‑150 (MQ=255)
                                            cgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCGAACCCACACCCACAGGGAGAGGGGACCg                                                                                                        >  1:7065963/1‑150 (MQ=255)
                                               aaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTc                                                                                                     <  1:6842951/150‑1 (MQ=255)
                                               aaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCGAAACCACTCCCACAGGGGGCGGGGGcccgta                                                                                                     >  2:3204940/1‑146 (MQ=255)
                                               aaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACTCCCACAGGGCGTGGGGACCGGTc                                                                                                     >  1:3965351/1‑150 (MQ=255)
                                                aTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCg                                                                                                    <  2:7400147/150‑1 (MQ=255)
                                                aTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAAACCACACCCGCACGGCGAGGGGACCGGTCg                                                                                                    >  1:8817247/1‑150 (MQ=255)
                                                 tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCAAACACACACACACACTGAGAGGGGTcccgtagg                                                                                                   >  1:5809521/1‑144 (MQ=255)
                                                  cGATCAGCGAACTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGGTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                                                  <  2:6850075/150‑1 (MQ=255)
                                                  cGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                                                  <  2:8442361/150‑1 (MQ=255)
                                                   gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                                                 <  2:1280290/150‑1 (MQ=255)
                                                     tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACTCCCACAGGGAGAGGGGACCCGTAGgcgcg                                                                                               >  1:2986611/1‑149 (MQ=255)
                                                      cAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                                              <  2:2371492/150‑1 (MQ=255)
                                                        gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACTCCCACAGGGAGAGGGGGCCGTTCGGCGCggta                                                                                            >  1:9637185/1‑150 (MQ=255)
                                                         cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtat                                                                                           <  2:8028571/150‑1 (MQ=255)
                                                            aaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCcgtatgta                                                                                        <  2:4977343/150‑1 (MQ=255)
                                                               tACGCAAGATTCGCTGGTGCAGGGGCTGGTTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGACGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTc                                                                                     <  1:6345677/150‑1 (MQ=255)
                                                               tACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCCAACCCACGCCCACACGGCGAGGGGACCCGTCGGCGCGGTATGTACTc                                                                                     >  1:6079837/1‑150 (MQ=255)
                                                                 cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTccc                                                                                   <  1:2055436/150‑1 (MQ=255)
                                                                  gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                                                  <  2:609410/150‑1 (MQ=255)
                                                                  gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTCGCCCCCCCCCCCCAACCCACACCCCCCGGGAGGGGGGTCCCGTCGGCGCggtatgtaagcccc                                                                                  >  1:5172428/1‑144 (MQ=255)
                                                                    aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCa                                                                                <  1:572597/150‑1 (MQ=255)
                                                                    aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCa                                                                                <  1:3609007/150‑1 (MQ=255)
                                                                     aGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCAc                                                                               <  2:2240931/150‑1 (MQ=255)
                                                                       aTTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCActc                                                                             <  1:3725984/150‑1 (MQ=255)
                                                                        ttCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACTACCACAGGGAGAGGGGCCCCTTAGGCGCAGTATGTACTCCCACAcact                                                                            >  2:5386813/1‑150 (MQ=255)
                                                                          cGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGa                                                                          <  2:3365450/150‑1 (MQ=255)
                                                                            cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                        <  2:3791389/150‑1 (MQ=255)
                                                                            cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                        <  1:3399576/150‑1 (MQ=255)
                                                                             tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCGCTCCCACAGGGGGAGGGGCCCCTTAGGCGCGGTTAGTACACCCCCACGCAGAAAc                                                                       >  2:1319615/1‑150 (MQ=255)
                                                                               gTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCCAAACCCCACCCAAAGGGCGGGGGGCCCCGGCGGCGCGGTTTGTACTCCCGCACTCAGAAACGa                                                                     >  2:946799/1‑150 (MQ=255)
                                                                                      ggCTGGGTAAAGTGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGTAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa                                                              <  1:1162231/150‑1 (MQ=255)
                                                                                       gCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCGAAACCACACCCACAGGGGGTCGGGACCCGGCGGCGCGGTATGGTCACCCACACACAGAAACGACAcccccc                                                             >  2:4107676/1‑146 (MQ=255)
                                                                                       gCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACGCCCCCACGGGGGGGGGGCCGGTCGGCGCGGGATGGTCGCCCGCACACGGAAACCCCCCCGCAc                                                             >  2:4840129/1‑150 (MQ=255)
                                                                                        cTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCTAACCCACTCCCTCCGGGGGAGGGGGCCGGTCGGCGGTGGATGTACTGCCGCACACTGTAACGACACCGCCct                                                            >  2:7140335/1‑150 (MQ=255)
                                                                                                agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGTAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTTAAACGACACCGCACTCttttttt                                                    <  1:1080719/150‑1 (MQ=255)
                                                                                                agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                    <  1:804166/150‑1 (MQ=255)
                                                                                                  agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCCCCCAAACCCACACCCCCACGGCGAGGGGGCCCGTGGGCGCGGGATTTTCGCCCACACACAGAAACCACACCGCCCGCattttttat                                                  >  1:8431529/1‑148 (MQ=255)
                                                                                                   gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                 <  2:432255/150‑1 (MQ=255)
                                                                                                    ttGCCTGCGGCACTGGCTCCCATGGCTAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                <  1:8563288/150‑1 (MQ=255)
                                                                                                    ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAACCTCTCCCACAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCACACACTGAAACGACACCGCACACtttttttatac                                                >  2:6333199/1‑146 (MQ=255)
                                                                                                    ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCTAAACCACACCCACAGGGCGTGGGGTCCGGTCGGCGCGGTATGTACACCCACACACAGAAACCACAACCCACGAGTTTTTTATcc                                                >  2:5976834/1‑150 (MQ=255)
                                                                                                        cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                            <  2:3071128/150‑1 (MQ=255)
                                                                                                        cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                            <  2:3519213/150‑1 (MQ=255)
                                                                                                        cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAAACCACGACCACAGGGGGAGGGGGCCCTTGGGCGCGGGGTGTAATCCCCCCCGCAGAAACGACACCCCCCACTTTTTTTATcccgcc                                            >  2:3909720/1‑147 (MQ=255)
                                                                                                          gCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc                                          <  2:2525255/150‑1 (MQ=255)
                                                                                                           cGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAAACCACACCCGCCGGGGGAGGGGGCCGTTCGGCGCGGTTTGTACTCCCACACTCTGAAAAGACACCGCACACTTTTTTTCTCCCGCGccc                                         >  2:1513401/1‑150 (MQ=255)
                                                                                                             gCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCCCCCCCACACCCAAAACCAAACCCACAGGGTGAGGGGGCCGTTCGGCGCGGGTTTTAAAACCACACACAGAAAACACACCGCACAAATTTTTTAACCCGCGcccca                                       >  2:813615/1‑149 (MQ=255)
                                                                                                              cACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTc                                      <  1:2188487/150‑1 (MQ=255)
                                                                                                              cACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAAACCACAACCGCAGGGAGAGGGGCCCCTTCGGCGCGGGATGTACTCCCACACTCGGAAACGACACCCCCCACGTTTTTTATCCCACGcccccc                                      >  2:7838218/1‑148 (MQ=255)
                                                                                                                 tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                   <  1:2844995/150‑1 (MQ=255)
                                                                                                                 tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACAGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                   <  2:9046453/150‑1 (MQ=255)
                                                                                                                  ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                  <  2:8686079/150‑1 (MQ=255)
                                                                                                                    cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                                <  1:1234714/150‑1 (MQ=255)
                                                                                                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                              <  1:4370165/150‑1 (MQ=255)
                                                                                                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                              <  1:9279838/150‑1 (MQ=255)
                                                                                                                      cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                              <  1:4792007/150‑1 (MQ=255)
                                                                                                                        cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                            <  2:2754180/150‑1 (MQ=255)
                                                                                                                         agggCGAACGGGCATGCGGCCGAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggg                           <  2:1029065/148‑1 (MQ=255)
                                                                                                                            gCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCAAACCAAAACCAAAACCCCACGGCGAGGGGACCCGTCGGCGCGGGGTGTACAACCACACACAGAAAACACACCCCCCAAATTTTTTATCACACCCCCCACCCGGGGGAggggcc                        >  2:2801781/1‑147 (MQ=255)
                                                                                                                             cGAATGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGTGGGGACCGTTCGGCGCTTTATGTACTCCCTAACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                       <  1:8178317/150‑1 (MQ=255)
                                                                                                                             cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                       <  1:7533109/150‑1 (MQ=255)
                                                                                                                              gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                      <  1:3107254/150‑1 (MQ=255)
                                                                                                                              gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGGACCCAAAACCTCTCCCACAGGGTGGGGGGGCCGGTGGGGGGGGTTTGTTGTGCCTGAAACTGTAAAGGAACCCCACAGTTTTTTTGTGACACGCCCCGCCGGGGGGAGGGGCggg                      >  2:325045/1‑150 (MQ=255)
                                                                                                                                   ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCATCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                 <  1:4225739/150‑1 (MQ=255)
                                                                                                                                          ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa          <  1:9411595/150‑1 (MQ=255)
                                                                                                                                           gCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAg         <  2:4597598/150‑1 (MQ=255)
                                                                                                                                                  cGTTTGCGCCCCCTCACCCTAACCCTCTACCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACACTGAAACGACACCGCACTCTTTTTTTATCCCTCGCCCCACCGGGGAGAGGGCCGGGGTGTGGGGAAAAGGCCGCAc  >  2:6998825/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCAC  >  NC_000913/3780873‑3781168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑